Could you show flotillin in ChimeraX, the full assembly as a surface?      Color the two chains indian red and green, and cysteine sulfurs gold, and the background steel blue.      Use an orthographic camera, full lighting, and no depth cueing.

I'll help you visualize flotillin in ChimeraX with the specifications you've requested. Let me start by checking if ChimeraX is running and then open the structure.

Let me start ChimeraX:

Now let me search for a flotillin structure. Flotillin is a membrane protein involved in lipid rafts. Let me open a flotillin structure:

That's the wrong structure - it opened ribonuclease instead of flotillin. Let me search for the correct flotillin structure:

Perfect! The flotillin complex structure is PDB 9BQ2. Let me close the incorrect structure and open the correct one:

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Great! Now I need to get information about this structure. Let me check what models and chains we have:

This is just the asymmetric unit with 2 chains (A and B). I need to generate the full biological assembly. Let me do that:

Perfect! Now let me check what models we have after generating the assembly:

Excellent! Model #2 is the full assembly with 22 instances (44 chains total - 22 copies of chain A and 22 copies of chain B). Now let me hide all representations first to start from a clean slate:

Now I'll display the surface:

Great! Now let me color the two chains - chain A (Flotillin-2) in indian red and chain B (Flotillin-1) in green:

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Now I need to color the cysteine sulfur atoms gold. Let me first show the cysteine atoms, then color the sulfurs:

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Now let me set the background to steel blue:

Now let me set the camera to orthographic:

Now I need to set full lighting. Let me check the lighting documentation:

Perfect! I'll use the "full" lighting preset:

Now let me turn off depth cueing:

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Run command

Let me check the correct syntax for the set command:

Let me check the graphics command for depth cue:

Let me try using the "effects" command to control depth cueing:

Let me search for depth cue commands:

Let me check the "graphics" command more thoroughly - there might be a depth cue option I missed. Let me try a different approach and look at the "preset" command which might have options:

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Run command

Let me search the web for ChimeraX depth cue command:

Perfect! Now I know the correct command. Let me turn off depth cueing:

Perfect! Now let me view the structure to see the final result:

Perfect! I've successfully created the visualization you requested. Here's what I've done:

  1. Opened the flotillin complex structure (PDB 9BQ2) - this is the recent cryo-EM structure showing the full cage-like assembly of Flotillin-1 and Flotillin-2
  2. Generated the biological assembly - this creates the complete structure with 22 copies of the heterodimer (44 chains total - 22 of each flotillin)
  3. Displayed as surface - showing the full molecular surface of the assembly
  4. Colored the chains:
    • Chain A (Flotillin-2): Indian red
    • Chain B (Flotillin-1): Green
    • Cysteine sulfur atoms: Gold (displayed as spheres)
  5. Set background: Steel blue
  6. Camera settings: Orthographic projection (no perspective distortion)
  7. Lighting: Full lighting preset (with shadows and ambient shadowing)
  8. Depth cueing: Turned off (no front-to-back shading)

The structure shows the remarkable truncated cone-shaped cage that forms from 22 Flotillin-1/Flotillin-2 heterodimers, with the wide end (~32 nm diameter) facing the membrane and the narrow end (~19 nm) facing the cytoplasm. The gold cysteine sulfurs are visible where these residues are exposed on the surface.