ChimeraX cryoEM Visualization Tutorial: Bacterial ATP Synthase

Tom Goddard
January 15, 2020

This ChimeraX tutorial will look at how to visualize atomic models and maps of three conformations of a bacterial ATP synthase determined by cryoEM at 3.0 and 3.2 Anstrom resolution. We will create views of the data similar to those found in

      Structure of a bacterial ATP synthase.
      Guo H, Suzuki T, Rubinstein JL.
      Elife. 2019 Feb 6;8. pii: e43128. doi: 10.7554/eLife.43128.

Atomic models PDB 6n2y, 6n2z, 6n30. EMDB maps 9333, 9334, 9335. ChimeraX can fetch these data files directly from the PDB and EMDB or you can download the files for offline use.

Command links in this page will execute in ChimeraX if this web page is displayed within ChimeraX. To show this tutorial in ChimeraX use menu entry Help / Documentation Index, choose Tutorials, and select the January 2020 cryoEM tutorial.


Opening PDB models

Display styles

Morphing between 3 conformations

Viewing cryoEM map near ADP

Inspecting side-chain density using zones

Fitting atomic model into map

Look at proton channels

Look at unidirectional rotation