# Sparky Tutorial and Reference Manual

Version 3.100, last revised June 18, 2001.

## Topics

- Introduction
- Spectrum Views
- Peaks and Assignments
- Extensions in Python
- Automatic Assignment
- Miscellaneous
- Files
- Getting Sparky
- Installation
- Acknowledgement
- New Features
- Command Index

## Overview

Sparky displays processed NMR spectra. You pick, assign, and integrate peaks using a graphical interface. You can work with any number of 2, 3 or 4 dimensional spectra simultaneously. The program has been developped to assist in structure determination of proteins, DNA and RNA. Spectra for input to Sparky can be produced with processing programs NMRPipe, Felix, VNMR, XWinNMR or UXNMR. Output consists of text peak lists showing assignments, chemical shifts, volumes, linewidths, .... Output suitable for structure determination with DYANA or XPLOR, or distance restraint calculation with MARDIGRAS can be generated.

You can get the latest html pages, images, and Postscript in tar or zip format from the Sparky web site. To search for specific words you may prefer the single file html manual. The manual is part of all Sparky distributions. It can be found in /usr/local/sparky/manual under Unix or Linux and in c:\Program Files\sparky\manual under Windows.