| 2716 | | |
| 2717 | | > hide #!3 models |
| 2718 | | |
| 2719 | | > hide #!2 models |
| 2720 | | |
| 2721 | | > hide #!1 models |
| 2722 | | |
| 2723 | | > show #!5 models |
| 2724 | | |
| 2725 | | > hide #!5 models |
| 2726 | | |
| 2727 | | > show #!4 models |
| 2728 | | |
| 2729 | | > volume #4 level 0.2842 |
| 2730 | | |
| 2731 | | > show #!3 models |
| 2732 | | |
| 2733 | | > hide #!4 models |
| 2734 | | |
| 2735 | | > volume #3 level 0.3546 |
| 2736 | | |
| 2737 | | > volume #3 level 0.356 |
| 2738 | | |
| 2739 | | > hide #!3 models |
| 2740 | | |
| 2741 | | > show #!1 models |
| 2742 | | |
| 2743 | | > show #!2 models |
| 2744 | | |
| 2745 | | > hide #!2 models |
| 2746 | | |
| 2747 | | > show #!5 models |
| 2748 | | |
| 2749 | | > hide #!1 models |
| 2750 | | |
| 2751 | | > color #1 #FCEDFF |
| 2752 | | |
| 2753 | | > hide #!5 models |
| 2754 | | |
| 2755 | | > show #!1 models |
| 2756 | | |
| 2757 | | > show #!2 models |
| 2758 | | |
| 2759 | | > hide #!1 models |
| 2760 | | |
| 2761 | | > show #!3 models |
| 2762 | | |
| 2763 | | > hide #!3 models |
| 2764 | | |
| 2765 | | > show #!3 models |
| 2766 | | |
| 2767 | | > hide #!2 models |
| 2768 | | |
| 2769 | | > show #!4 models |
| 2770 | | |
| 2771 | | > hide #!3 models |
| 2772 | | |
| 2773 | | > hide #!4 models |
| 2774 | | |
| 2775 | | > show #!5 models |
| 2776 | | |
| 2777 | | > hide #!5 models |
| 2778 | | |
| 2779 | | > show #!1 models |
| 2780 | | |
| 2781 | | > volume flip #1 |
| 2782 | | |
| 2783 | | Opened cryosparc_P4_J216_class_00_00122_volume_sharp.mrc z flip as #6, grid |
| 2784 | | size 128,128,128, pixel 2.08, shown at step 1, values float32 |
| 2785 | | |
| 2786 | | > hide #!6 models |
| 2787 | | |
| 2788 | | > show #!2 models |
| 2789 | | |
| 2790 | | > volume flip #2 |
| 2791 | | |
| 2792 | | Opened cryosparc_P4_J216_class_01_00122_volume_sharp.mrc z flip as #7, grid |
| 2793 | | size 128,128,128, pixel 2.08, shown at step 1, values float32 |
| 2794 | | |
| 2795 | | > hide #!7 models |
| 2796 | | |
| 2797 | | > show #!1 models |
| 2798 | | |
| 2799 | | > hide #!1 models |
| 2800 | | |
| 2801 | | > show #!2 models |
| 2802 | | |
| 2803 | | > hide #!2 models |
| 2804 | | |
| 2805 | | > show #!3 models |
| 2806 | | |
| 2807 | | > hide #!3 models |
| 2808 | | |
| 2809 | | > volume flip #3 |
| 2810 | | |
| 2811 | | Opened cryosparc_P4_J216_class_02_00122_volume_sharp.mrc z flip as #8, grid |
| 2812 | | size 128,128,128, pixel 2.08, shown at step 1, values float32 |
| 2813 | | |
| 2814 | | > show #!4 models |
| 2815 | | |
| 2816 | | > hide #!4 models |
| 2817 | | |
| 2818 | | > show #!5 models |
| 2819 | | |
| 2820 | | > hide #!8 models |
| 2821 | | |
| 2822 | | > hide #!5 models |
| 2823 | | |
| 2824 | | > close #1 |
| 2825 | | |
| 2826 | | > close #2 |
| 2827 | | |
| 2828 | | > close #3 |
| 2829 | | |
| 2830 | | > rename #6 CS_P4_J216_class_0_sharp_flip id #1 |
| 2831 | | |
| 2832 | | > save #1 CS_P4_J216_class_0_sharp_flip.mrc |
| 2833 | | |
| 2834 | | Cannot determine format for '#1' |
| 2835 | | |
| 2836 | | > save CS_P4_J216_class_0_sharp_flip.mrc #1 |
| 2837 | | |
| 2838 | | > rename #7 CS_P4_J216_class_1_sharp_flip id #1_sharp_flip.mrc #2 |
| 2839 | | |
| 2840 | | Invalid "id" argument: Expected an integer |
| 2841 | | |
| 2842 | | > rename #7 CS_P4_J216_class_1_sharp_flip id #1_sharp_flip id #2 |
| 2843 | | |
| 2844 | | Invalid "id" argument: Expected an integer |
| 2845 | | |
| 2846 | | > rename #7 CS_P4_J216_class_2_sharp_flip id #2 |
| 2847 | | |
| 2848 | | > rename #2 CS_P4_J216_class_1_sharp_flip |
| 2849 | | |
| 2850 | | > rename #8 CS_P4_J216_class_2_sharp_flip id #3 |
| 2851 | | |
| 2852 | | > save CS_P4_J216_class_1_sharp_flip.mrc #2 |
| 2853 | | |
| 2854 | | > save CS_P4_J216_class_2_sharp_flip.mrc #3 |
| 2855 | | |
| 2856 | | > save "/Users/peiyuyu/Documents/Goethe |
| 2857 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 2858 | | > F_B1/20260430_SA-F_B1_workflow.cxs" |
| 2859 | | |
| 2860 | | > show #!1 models |
| 2861 | | |
| 2862 | | > show #!2 models |
| 2863 | | |
| 2864 | | > show #!3 models |
| 2865 | | |
| 2866 | | > show #!4 models |
| 2867 | | |
| 2868 | | > show #!5 models |
| 2869 | | |
| 2870 | | > hide #!4 models |
| 2871 | | |
| 2872 | | > hide #!3 models |
| 2873 | | |
| 2874 | | > hide #!2 models |
| 2875 | | |
| 2876 | | > hide #!1 models |
| 2877 | | |
| 2878 | | > show #!3 models |
| 2879 | | |
| 2880 | | > hide #!3 models |
| 2881 | | |
| 2882 | | > show #!4 models |
| 2883 | | |
| 2884 | | > hide #!5 models |
| 2885 | | |
| 2886 | | > hide #!4 models |
| 2887 | | |
| 2888 | | > show #!3 models |
| 2889 | | |
| 2890 | | > hide #!3 models |
| 2891 | | |
| 2892 | | > show #!2 models |
| 2893 | | |
| 2894 | | > hide #!2 models |
| 2895 | | |
| 2896 | | > show #!5 models |
| 2897 | | |
| 2898 | | > show #!3 models |
| 2899 | | |
| 2900 | | > hide #!5 models |
| 2901 | | |
| 2902 | | > show #!1 models |
| 2903 | | |
| 2904 | | > select add #1 |
| 2905 | | |
| 2906 | | 2 models selected |
| 2907 | | |
| 2908 | | > ui mousemode right "translate selected models" |
| 2909 | | |
| 2910 | | > view matrix models #1,1,0,0,-2.4868,0,1,0,6.8233,0,0,1,22.302 |
| 2911 | | |
| 2912 | | > ui mousemode right "rotate selected models" |
| 2913 | | |
| 2914 | | > view matrix models |
| 2915 | | > #1,0.85834,-0.51214,-0.03115,83.967,-0.50924,-0.85776,0.070198,298.29,-0.06267,-0.044391,-0.99705,270.86 |
| 2916 | | |
| 2917 | | Must specify one map, got 0 |
| 2918 | | |
| 2919 | | > fitmap #1 inMap #3 |
| 2920 | | |
| 2921 | | Fit map CS_P4_J216_class_0_sharp_flip.mrc in map |
| 2922 | | CS_P4_J216_class_2_sharp_flip.mrc using 20952 points |
| 2923 | | correlation = 0.8163, correlation about mean = 0.111, overlap = 1135 |
| 2924 | | steps = 116, shift = 13.1, angle = 14.8 degrees |
| 2925 | | |
| 2926 | | Position of CS_P4_J216_class_0_sharp_flip.mrc (#1) relative to |
| 2927 | | CS_P4_J216_class_2_sharp_flip.mrc (#3) coordinates: |
| 2928 | | Matrix rotation and translation |
| 2929 | | 0.94377261 -0.32509802 0.06003771 31.40521096 |
| 2930 | | -0.32212557 -0.94514622 -0.05416389 308.66342237 |
| 2931 | | 0.07435299 0.03177871 -0.99672551 251.62777867 |
| 2932 | | Axis 0.98583616 -0.16420866 0.03409658 |
| 2933 | | Axis point 0.00000000 154.16030965 128.66405805 |
| 2934 | | Rotation angle (degrees) 177.50176084 |
| 2935 | | Shift along axis -11.14516800 |
| 2936 | | |
| 2937 | | |
| 2938 | | > select subtract #1 |
| 2939 | | |
| 2940 | | Nothing selected |
| 2941 | | |
| 2942 | | > hide #!3 models |
| 2943 | | |
| 2944 | | > hide #!1 models |
| 2945 | | |
| 2946 | | > show #!2 models |
| 2947 | | |
| 2948 | | > show #!3 models |
| 2949 | | |
| 2950 | | > fitmap #2 inMap #3 |
| 2951 | | |
| 2952 | | Fit map CS_P4_J216_class_1_sharp_flip.mrc in map |
| 2953 | | CS_P4_J216_class_2_sharp_flip.mrc using 20964 points |
| 2954 | | correlation = 0.8775, correlation about mean = 0.2387, overlap = 1535 |
| 2955 | | steps = 72, shift = 7.04, angle = 9.35 degrees |
| 2956 | | |
| 2957 | | Position of CS_P4_J216_class_1_sharp_flip.mrc (#2) relative to |
| 2958 | | CS_P4_J216_class_2_sharp_flip.mrc (#3) coordinates: |
| 2959 | | Matrix rotation and translation |
| 2960 | | 0.99017956 0.04949629 -0.13074615 12.42570756 |
| 2961 | | -0.06022022 0.99502365 -0.07938169 18.18421737 |
| 2962 | | 0.12616642 0.08647569 0.98823276 -19.36289944 |
| 2963 | | Axis 0.51051058 -0.79077932 -0.33770846 |
| 2964 | | Axis point 165.72686650 0.00000000 88.43510724 |
| 2965 | | Rotation angle (degrees) 9.34870453 |
| 2966 | | Shift along axis -1.49723285 |
| 2967 | | |
| 2968 | | |
| 2969 | | > hide #!2 models |
| 2970 | | |
| 2971 | | > show #!4 models |
| 2972 | | |
| 2973 | | > fitmap #4 inMap #3 |
| 2974 | | |
| 2975 | | Fit map cryosparc_P4_J216_class_03_00122_volume_sharp.mrc in map |
| 2976 | | CS_P4_J216_class_2_sharp_flip.mrc using 5887 points |
| 2977 | | correlation = 0.7186, correlation about mean = 0.04573, overlap = 331.9 |
| 2978 | | steps = 104, shift = 11.1, angle = 13.1 degrees |
| 2979 | | |
| 2980 | | Position of cryosparc_P4_J216_class_03_00122_volume_sharp.mrc (#4) relative to |
| 2981 | | CS_P4_J216_class_2_sharp_flip.mrc (#3) coordinates: |
| 2982 | | Matrix rotation and translation |
| 2983 | | 0.97455290 -0.13512397 0.17885231 -1.53434267 |
| 2984 | | 0.14053006 0.98991548 -0.01785084 -17.74958210 |
| 2985 | | -0.17463660 0.04253071 0.98371398 30.92421506 |
| 2986 | | Axis 0.13349556 0.78151692 0.60943437 |
| 2987 | | Axis point 157.21397711 0.00000000 34.50744362 |
| 2988 | | Rotation angle (degrees) 13.07084305 |
| 2989 | | Shift along axis 4.76985279 |
| 2990 | | |
| 2991 | | |
| 2992 | | > hide #!4 models |
| 2993 | | |
| 2994 | | > show #!5 models |
| 2995 | | |
| 2996 | | > select add #5 |
| 2997 | | |
| 2998 | | 2 models selected |
| 2999 | | |
| 3000 | | > view matrix models |
| 3001 | | > #5,0.70232,-0.70945,-0.058532,142.48,-0.70661,-0.70474,0.063551,312.49,-0.086337,-0.0032735,-0.99626,292.78 |
| 3002 | | |
| 3003 | | > fitmap #5 inMap #3 |
| 3004 | | |
| 3005 | | Fit map cryosparc_P4_J216_class_04_00122_volume_sharp.mrc in map |
| 3006 | | CS_P4_J216_class_2_sharp_flip.mrc using 20952 points |
| 3007 | | correlation = 0.896, correlation about mean = 0.3255, overlap = 1903 |
| 3008 | | steps = 100, shift = 4.43, angle = 13 degrees |
| 3009 | | |
| 3010 | | Position of cryosparc_P4_J216_class_04_00122_volume_sharp.mrc (#5) relative to |
| 3011 | | CS_P4_J216_class_2_sharp_flip.mrc (#3) coordinates: |
| 3012 | | Matrix rotation and translation |
| 3013 | | 0.72688934 -0.68457986 0.05461051 119.19907428 |
| 3014 | | -0.67457364 -0.72664609 -0.13013783 337.91149541 |
| 3015 | | 0.12877226 0.05775699 -0.98999082 252.38058253 |
| 3016 | | Axis 0.92902840 -0.36668580 0.04947481 |
| 3017 | | Axis point 0.00000000 185.59312596 132.22855036 |
| 3018 | | Rotation angle (degrees) 174.19607958 |
| 3019 | | Shift along axis -0.68154259 |
| 3020 | | |
| 3021 | | |
| 3022 | | > select subtract #5 |
| 3023 | | |
| 3024 | | Nothing selected |
| 3025 | | |
| 3026 | | > save "/Users/peiyuyu/Documents/Goethe |
| 3027 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 3028 | | > F_B1/20260430_SA-F_B1_workflow.cxs" |
| 3029 | | |
| 3030 | | > hide #!5 models |
| 3031 | | |
| 3032 | | > show #!5 models |
| 3033 | | |
| 3034 | | > hide #!3 models |
| 3035 | | |
| 3036 | | > show #!1 models |
| 3037 | | |
| 3038 | | > hide #!5 models |
| 3039 | | |
| 3040 | | > hide #!1 models |
| 3041 | | |
| 3042 | | > show #!2 models |
| 3043 | | |
| 3044 | | > color #2 #FCEDFF |
| 3045 | | |
| 3046 | | > hide #!2 models |
| 3047 | | |
| 3048 | | > show #!4 models |
| 3049 | | |
| 3050 | | > color #4 #FCEDFF |
| 3051 | | |
| 3052 | | > hide #!4 models |
| 3053 | | |
| 3054 | | > show #!5 models |
| 3055 | | |
| 3056 | | > color #5 #FCEDFF |
| 3057 | | |
| 3058 | | > hide #!5 models |
| 3059 | | |
| 3060 | | > show #!3 models |
| 3061 | | |
| 3062 | | > color #5 #E9C29B |
| 3063 | | |
| 3064 | | > color #5 #FCEDFF |
| 3065 | | |
| 3066 | | > color #3 #E9C29B |
| 3067 | | |
| 3068 | | > ui mousemode right translate |
| 3069 | | |
| 3070 | | > volume #2 level 0.356 |
| 3071 | | |
| 3072 | | > volume #1 level 0.356 |
| 3073 | | |
| 3074 | | > volume #4 level 0.356 |
| 3075 | | |
| 3076 | | > volume #5 level 0.356 |
| 3077 | | |
| 3078 | | > hide #!3 models |
| 3079 | | |
| 3080 | | > show #!1 models |
| 3081 | | |
| 3082 | | > undo |
| 3083 | | |
| 3084 | | [Repeated 1 time(s)] |
| 3085 | | |
| 3086 | | > hide #!3 models |
| 3087 | | |
| 3088 | | > show #!1 models |
| 3089 | | |
| 3090 | | > hide #!1 models |
| 3091 | | |
| 3092 | | > show #!1 models |
| 3093 | | |
| 3094 | | > hide #!1 models |
| 3095 | | |
| 3096 | | > show #!1 models |
| 3097 | | |
| 3098 | | > hide #!1 models |
| 3099 | | |
| 3100 | | > show #!1 models |
| 3101 | | |
| 3102 | | > hide #!1 models |
| 3103 | | |
| 3104 | | > show #!1 models |
| 3105 | | |
| 3106 | | > volume #1 level 0.1727 |
| 3107 | | |
| 3108 | | > volume #1 level 0.1851 |
| 3109 | | |
| 3110 | | > show #!2 models |
| 3111 | | |
| 3112 | | > hide #!1 models |
| 3113 | | |
| 3114 | | > volume #2 level 0.203 |
| 3115 | | |
| 3116 | | > show #!3 models |
| 3117 | | |
| 3118 | | > hide #!2 models |
| 3119 | | |
| 3120 | | > hide #!3 models |
| 3121 | | |
| 3122 | | > show #!4 models |
| 3123 | | |
| 3124 | | > volume #4 level 0.1532 |
| 3125 | | |
| 3126 | | > show #!3 models |
| 3127 | | |
| 3128 | | > select add #4 |
| 3129 | | |
| 3130 | | 2 models selected |
| 3131 | | |
| 3132 | | > ui mousemode right "translate selected models" |
| 3133 | | |
| 3134 | | > view matrix models |
| 3135 | | > #4,0.97455,-0.13512,0.17885,1.3208,0.14053,0.98992,-0.017851,-10.868,-0.17464,0.042531,0.98371,31.337 |
| 3136 | | |
| 3137 | | > ui mousemode right "rotate selected models" |
| 3138 | | |
| 3139 | | > view matrix models |
| 3140 | | > #4,-0.085498,-0.64922,0.75578,120.23,0.99605,-0.074063,0.049058,8.8958,0.024125,0.75698,0.65299,-39.323 |
| 3141 | | |
| 3142 | | > ui mousemode right "rotate selected models" |
| 3143 | | |
| 3144 | | > ui mousemode right "translate selected models" |
| 3145 | | |
| 3146 | | > view matrix models |
| 3147 | | > #4,-0.085498,-0.64922,0.75578,134.23,0.99605,-0.074063,0.049058,6.1939,0.024125,0.75698,0.65299,-87.477 |
| 3148 | | |
| 3149 | | > view matrix models |
| 3150 | | > #4,-0.085498,-0.64922,0.75578,134.33,0.99605,-0.074063,0.049058,6.22,0.024125,0.75698,0.65299,-87.742 |
| 3151 | | |
| 3152 | | > view matrix models |
| 3153 | | > #4,-0.085498,-0.64922,0.75578,130.4,0.99605,-0.074063,0.049058,5.0583,0.024125,0.75698,0.65299,-76.883 |
| 3154 | | |
| 3155 | | > fitmap #4 inMap #3 |
| 3156 | | |
| 3157 | | Fit map cryosparc_P4_J216_class_03_00122_volume_sharp.mrc in map |
| 3158 | | CS_P4_J216_class_2_sharp_flip.mrc using 42435 points |
| 3159 | | correlation = 0.7796, correlation about mean = 0.1614, overlap = 1558 |
| 3160 | | steps = 228, shift = 2.07, angle = 23.4 degrees |
| 3161 | | |
| 3162 | | Position of cryosparc_P4_J216_class_03_00122_volume_sharp.mrc (#4) relative to |
| 3163 | | CS_P4_J216_class_2_sharp_flip.mrc (#3) coordinates: |
| 3164 | | Matrix rotation and translation |
| 3165 | | -0.31733316 -0.35582789 0.87902570 104.58434579 |
| 3166 | | 0.93180439 -0.28920783 0.21931579 18.83015631 |
| 3167 | | 0.17618244 0.88867618 0.42333722 -81.48445272 |
| 3168 | | Axis 0.41511738 0.43588247 0.79855121 |
| 3169 | | Axis point 65.64014873 66.59534740 0.00000000 |
| 3170 | | Rotation angle (degrees) 126.27076538 |
| 3171 | | Shift along axis -13.44699307 |
| 3172 | | |
| 3173 | | |
| 3174 | | > ui mousemode right "rotate selected models" |
| 3175 | | |
| 3176 | | > view matrix models |
| 3177 | | > #4,-0.85396,0.083596,-0.51358,313.46,-0.52034,-0.13698,0.8429,94.578,0.00011363,0.98704,0.16047,-34.579 |
| 3178 | | |
| 3179 | | > fitmap #4 inMap #3 |
| 3180 | | |
| 3181 | | Fit map cryosparc_P4_J216_class_03_00122_volume_sharp.mrc in map |
| 3182 | | CS_P4_J216_class_2_sharp_flip.mrc using 42435 points |
| 3183 | | correlation = 0.7628, correlation about mean = 0.1138, overlap = 1507 |
| 3184 | | steps = 160, shift = 8.1, angle = 11.6 degrees |
| 3185 | | |
| 3186 | | Position of cryosparc_P4_J216_class_03_00122_volume_sharp.mrc (#4) relative to |
| 3187 | | CS_P4_J216_class_2_sharp_flip.mrc (#3) coordinates: |
| 3188 | | Matrix rotation and translation |
| 3189 | | -0.75756109 0.20637267 -0.61928307 295.50473254 |
| 3190 | | -0.63935005 -0.04324082 0.76769900 115.47474727 |
| 3191 | | 0.13165378 0.97751755 0.16470194 -51.62941296 |
| 3192 | | Axis 0.18240424 -0.65282152 -0.73522293 |
| 3193 | | Axis point 168.50534676 17.73009752 0.00000000 |
| 3194 | | Rotation angle (degrees) 144.89006320 |
| 3195 | | Shift along axis 16.47604383 |
| 3196 | | |
| 3197 | | |
| 3198 | | > select subtract #4 |
| 3199 | | |
| 3200 | | Nothing selected |
| 3201 | | |
| 3202 | | > volume #4 level 0.3211 |
| 3203 | | |
| 3204 | | > hide #!3 models |
| 3205 | | |
| 3206 | | > volume #4 level 0.2347 |
| 3207 | | |
| 3208 | | > volume #4 level 0.2031 |
| 3209 | | |
| 3210 | | > hide #!4 models |
| 3211 | | |
| 3212 | | > show #!5 models |
| 3213 | | |
| 3214 | | > volume #5 level 0.1924 |
| 3215 | | |
| 3216 | | > volume #5 level 0.2064 |
| 3217 | | |
| 3218 | | > ui mousemode right translate |
| 3219 | | |
| 3220 | | > show #!3 models |
| 3221 | | |
| 3222 | | > hide #!5 models |
| 3223 | | |
| 3224 | | > color #3 #B9DBD5 |
| 3225 | | |
| 3226 | | > hide #!3 models |
| 3227 | | |
| 3228 | | > show #!1 models |
| 3229 | | |
| 3230 | | > lighting shadows true intensity 0.5 |
| 3231 | | |
| 3232 | | > lighting flat |
| 3233 | | |
| 3234 | | > graphics silhouettes false |
| 3235 | | |
| 3236 | | > graphics silhouettes true |
| 3237 | | |
| 3238 | | > lighting soft |
| 3239 | | |
| 3240 | | > save P4_J216_class_0_sharp.png supersample 3 transparentBackground true |
| 3241 | | |
| 3242 | | > hide #!1 models |
| 3243 | | |
| 3244 | | > show #!2 models |
| 3245 | | |
| 3246 | | > save P4_J216_class_1_sharp.png supersample 3 transparentBackground true |
| 3247 | | |
| 3248 | | > hide #!2 models |
| 3249 | | |
| 3250 | | > show #!3 models |
| 3251 | | |
| 3252 | | > save P4_J216_class_2_sharp.png supersample 3 transparentBackground true |
| 3253 | | |
| 3254 | | > hide #!3 models |
| 3255 | | |
| 3256 | | > show #!4 models |
| 3257 | | |
| 3258 | | > save P4_J216_class_3_sharp.png supersample 3 transparentBackground true |
| 3259 | | |
| 3260 | | > hide #!4 models |
| 3261 | | |
| 3262 | | > show #!5 models |
| 3263 | | |
| 3264 | | > show #!1 models |
| 3265 | | |
| 3266 | | > show #!2 models |
| 3267 | | |
| 3268 | | > show #!3 models |
| 3269 | | |
| 3270 | | > show #!4 models |
| 3271 | | |
| 3272 | | > hide #!4 models |
| 3273 | | |
| 3274 | | > hide #!5 models |
| 3275 | | |
| 3276 | | > show #!5 models |
| 3277 | | |
| 3278 | | > hide #!5 models |
| 3279 | | |
| 3280 | | > hide #!3 models |
| 3281 | | |
| 3282 | | > hide #!2 models |
| 3283 | | |
| 3284 | | > hide #!1 models |
| 3285 | | |
| 3286 | | > show #!1 models |
| 3287 | | |
| 3288 | | > view name P1 |
| 3289 | | |
| 3290 | | [Repeated 1 time(s)] |
| 3291 | | |
| 3292 | | > save P4_J216_class_0_sharp.png supersample 3 transparentBackground true |
| 3293 | | |
| 3294 | | > hide #!1 models |
| 3295 | | |
| 3296 | | > show #!2 models |
| 3297 | | |
| 3298 | | > save P4_J216_class_1_sharp.png supersample 3 transparentBackground true |
| 3299 | | |
| 3300 | | > hide #!2 models |
| 3301 | | |
| 3302 | | > show #!3 models |
| 3303 | | |
| 3304 | | > hide #!3 models |
| 3305 | | |
| 3306 | | > show #!2 models |
| 3307 | | |
| 3308 | | > hide #!2 models |
| 3309 | | |
| 3310 | | > show #!1 models |
| 3311 | | |
| 3312 | | > hide #!1 models |
| 3313 | | |
| 3314 | | > show #!2 models |
| 3315 | | |
| 3316 | | > hide #!2 models |
| 3317 | | |
| 3318 | | > show #!3 models |
| 3319 | | |
| 3320 | | > save P4_J216_class_2_sharp.png supersample 3 transparentBackground true |
| 3321 | | |
| 3322 | | > hide #!3 models |
| 3323 | | |
| 3324 | | > show #!4 models |
| 3325 | | |
| 3326 | | > hide #!4 models |
| 3327 | | |
| 3328 | | > show #!5 models |
| 3329 | | |
| 3330 | | > hide #!5 models |
| 3331 | | |
| 3332 | | > show #!4 models |
| 3333 | | |
| 3334 | | > save P4_J216_class_3_sharp.png supersample 3 transparentBackground true |
| 3335 | | |
| 3336 | | > hide #!4 models |
| 3337 | | |
| 3338 | | > show #!5 models |
| 3339 | | |
| 3340 | | > vieww P1 |
| 3341 | | |
| 3342 | | Unknown command: vieww P1 |
| 3343 | | |
| 3344 | | > view P1 |
| 3345 | | |
| 3346 | | [Repeated 1 time(s)] |
| 3347 | | |
| 3348 | | > save P4_J216_class_4_sharp.png supersample 3 transparentBackground true |
| 3349 | | |
| 3350 | | > save "/Users/peiyuyu/Documents/Goethe |
| 3351 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 3352 | | > F_B1/20260430_SA-F_B1_workflow.cxs" |
| 3353 | | |
| 3354 | | > open "/Users/peiyuyu/Documents/Goethe |
| 3355 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 3356 | | > F_B1/cryosparc_P4_J219_002_volume_map_sharp.mrc" |
| 3357 | | |
| 3358 | | Opened cryosparc_P4_J219_002_volume_map_sharp.mrc as #6, grid size 80,80,80, |
| 3359 | | pixel 3.32, shown at level 0.854, step 1, values float32 |
| 3360 | | |
| 3361 | | > hide #!5 models |
| 3362 | | |
| 3363 | | > show #!3 models |
| 3364 | | |
| 3365 | | > select add #6 |
| 3366 | | |
| 3367 | | 2 models selected |
| 3368 | | |
| 3369 | | > ui mousemode right "rotate selected models" |
| 3370 | | |
| 3371 | | > view matrix models |
| 3372 | | > #6,0.50968,-0.8597,0.033732,182.49,-0.85693,-0.50376,0.10903,313.05,-0.076743,-0.084479,-0.99347,263.76 |
| 3373 | | |
| 3374 | | > ui mousemode right "translate selected models" |
| 3375 | | |
| 3376 | | > view matrix models |
| 3377 | | > #6,0.50968,-0.8597,0.033732,181.37,-0.85693,-0.50376,0.10903,307.2,-0.076743,-0.084479,-0.99347,286.3 |
| 3378 | | |
| 3379 | | > fitmap #6 inMap #3 |
| 3380 | | |
| 3381 | | Fit map cryosparc_P4_J219_002_volume_map_sharp.mrc in map |
| 3382 | | CS_P4_J216_class_2_sharp_flip.mrc using 5120 points |
| 3383 | | correlation = 0.7368, correlation about mean = 0.07448, overlap = 1741 |
| 3384 | | steps = 88, shift = 8.42, angle = 12.4 degrees |
| 3385 | | |
| 3386 | | Position of cryosparc_P4_J219_002_volume_map_sharp.mrc (#6) relative to |
| 3387 | | CS_P4_J216_class_2_sharp_flip.mrc (#3) coordinates: |
| 3388 | | Matrix rotation and translation |
| 3389 | | 0.68179907 -0.73031354 0.04233404 134.55628275 |
| 3390 | | -0.72995927 -0.67538450 0.10495351 308.10402853 |
| 3391 | | -0.04805721 -0.10245933 -0.99357566 283.32032359 |
| 3392 | | Axis -0.91672636 0.39951261 0.00156579 |
| 3393 | | Axis point 0.00000000 192.14085888 132.24148806 |
| 3394 | | Rotation angle (degrees) 173.50439997 |
| 3395 | | Shift along axis 0.18377410 |
| 3396 | | |
| 3397 | | |
| 3398 | | > volume #6 level 1.828 |
| 3399 | | |
| 3400 | | > select subtract #6 |
| 3401 | | |
| 3402 | | Nothing selected |
| 3403 | | |
| 3404 | | > hide #!3 models |
| 3405 | | |
| 3406 | | > volume #6 level 1.172 |
| 3407 | | |
| 3408 | | > show #!3 models |
| 3409 | | |
| 3410 | | > select add #6 |
| 3411 | | |
| 3412 | | 2 models selected |
| 3413 | | |
| 3414 | | > view matrix models |
| 3415 | | > #6,0.6818,-0.73031,0.042334,145.51,-0.72996,-0.67538,0.10495,294.75,-0.048057,-0.10246,-0.99358,285.84 |
| 3416 | | |
| 3417 | | > view matrix models |
| 3418 | | > #6,0.6818,-0.73031,0.042334,133.79,-0.72996,-0.67538,0.10495,309.04,-0.048057,-0.10246,-0.99358,282.76 |
| 3419 | | |
| 3420 | | > ui mousemode right "rotate selected models" |
| 3421 | | |
| 3422 | | > view matrix models |
| 3423 | | > #6,0.47143,0.87224,0.13024,-67.58,0.87592,-0.44591,-0.18423,88.863,-0.10262,0.20093,-0.97422,246.36 |
| 3424 | | |
| 3425 | | > fitmap #6 inMap #3 |
| 3426 | | |
| 3427 | | Fit map cryosparc_P4_J219_002_volume_map_sharp.mrc in map |
| 3428 | | CS_P4_J216_class_2_sharp_flip.mrc using 3507 points |
| 3429 | | correlation = 0.7523, correlation about mean = 0.053, overlap = 1436 |
| 3430 | | steps = 84, shift = 11, angle = 9.96 degrees |
| 3431 | | |
| 3432 | | Position of cryosparc_P4_J219_002_volume_map_sharp.mrc (#6) relative to |
| 3433 | | CS_P4_J216_class_2_sharp_flip.mrc (#3) coordinates: |
| 3434 | | Matrix rotation and translation |
| 3435 | | 0.31271016 0.94213099 0.12083688 -48.05138587 |
| 3436 | | 0.94223385 -0.29160641 -0.16480619 65.68302492 |
| 3437 | | -0.12003221 0.16539317 -0.97889599 255.74245569 |
| 3438 | | Axis 0.80789250 0.58932983 0.00025166 |
| 3439 | | Axis point 0.00000000 34.02227920 132.07845223 |
| 3440 | | Rotation angle (degrees) 168.20805262 |
| 3441 | | Shift along axis -0.04702893 |
| 3442 | | |
| 3443 | | |
| 3444 | | > select subtract #6 |
| 3445 | | |
| 3446 | | Nothing selected |
| 3447 | | |
| 3448 | | > hide #!6 models |
| 3449 | | |
| 3450 | | > show #!6 models |
| 3451 | | |
| 3452 | | > select add #6 |
| 3453 | | |
| 3454 | | 2 models selected |
| 3455 | | |
| 3456 | | > view matrix models |
| 3457 | | > #6,-0.96774,-0.18791,0.16785,278.55,-0.18769,0.98208,0.017294,25.636,-0.16809,-0.014768,-0.98566,287.91 |
| 3458 | | |
| 3459 | | > ui mousemode right "translate selected models" |
| 3460 | | |
| 3461 | | > view matrix models |
| 3462 | | > #6,-0.96774,-0.18791,0.16785,272.6,-0.18769,0.98208,0.017294,32.876,-0.16809,-0.014768,-0.98566,284.26 |
| 3463 | | |
| 3464 | | > fitmap #6 inMap #3 |
| 3465 | | |
| 3466 | | Fit map cryosparc_P4_J219_002_volume_map_sharp.mrc in map |
| 3467 | | CS_P4_J216_class_2_sharp_flip.mrc using 3507 points |
| 3468 | | correlation = 0.7248, correlation about mean = 0.05418, overlap = 1264 |
| 3469 | | steps = 60, shift = 9.72, angle = 10.6 degrees |
| 3470 | | |
| 3471 | | Position of cryosparc_P4_J219_002_volume_map_sharp.mrc (#6) relative to |
| 3472 | | CS_P4_J216_class_2_sharp_flip.mrc (#3) coordinates: |
| 3473 | | Matrix rotation and translation |
| 3474 | | -0.92647017 -0.30027989 0.22690311 266.55888821 |
| 3475 | | -0.26721766 0.94935570 0.16528304 28.41095713 |
| 3476 | | -0.26504293 0.09249729 -0.95978982 282.00607809 |
| 3477 | | Axis -0.14603906 0.98705219 0.06633684 |
| 3478 | | Axis point 153.43261550 0.00000000 122.88099835 |
| 3479 | | Rotation angle (degrees) 165.56984584 |
| 3480 | | Shift along axis 7.82248126 |
| 3481 | | |
| 3482 | | |
| 3483 | | > ui mousemode right "rotate selected models" |
| 3484 | | |
| 3485 | | > view matrix models |
| 3486 | | > #6,-0.92785,-0.005043,0.37292,209.61,0.027742,0.99621,0.082497,-9.3167,-0.37192,0.086891,-0.92419,293.48 |
| 3487 | | |
| 3488 | | > fitmap #6 inMap #3 |
| 3489 | | |
| 3490 | | Fit map cryosparc_P4_J219_002_volume_map_sharp.mrc in map |
| 3491 | | CS_P4_J216_class_2_sharp_flip.mrc using 3507 points |
| 3492 | | correlation = 0.7519, correlation about mean = 0.05711, overlap = 1436 |
| 3493 | | steps = 100, shift = 5.47, angle = 14.5 degrees |
| 3494 | | |
| 3495 | | Position of cryosparc_P4_J219_002_volume_map_sharp.mrc (#6) relative to |
| 3496 | | CS_P4_J216_class_2_sharp_flip.mrc (#3) coordinates: |
| 3497 | | Matrix rotation and translation |
| 3498 | | -0.92643320 -0.21423055 0.30955905 249.03084383 |
| 3499 | | -0.21368345 0.97623560 0.03610312 27.35482068 |
| 3500 | | -0.30993696 -0.03270052 -0.95019459 303.95121642 |
| 3501 | | Axis -0.11038513 0.99388850 0.00087774 |
| 3502 | | Axis point 150.47041621 0.00000000 131.94319456 |
| 3503 | | Rotation angle (degrees) 161.84114382 |
| 3504 | | Shift along axis -0.03486954 |
| 3505 | | |
| 3506 | | |
| 3507 | | > select subtract #6 |
| 3508 | | |
| 3509 | | Nothing selected |
| 3510 | | |
| 3511 | | > hide #!3 models |
| 3512 | | |
| 3513 | | > hide #!6 models |
| 3514 | | |
| 3515 | | > show #!3 models |
| 3516 | | |
| 3517 | | > hide #!3 models |
| 3518 | | |
| 3519 | | > show #!6 models |
| 3520 | | |
| 3521 | | > volume flip #6 |
| 3522 | | |
| 3523 | | Opened cryosparc_P4_J219_002_volume_map_sharp.mrc z flip as #7, grid size |
| 3524 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 3525 | | |
| 3526 | | > close #6 |
| 3527 | | |
| 3528 | | > rename CS_P4_J219_sharp_flip id #6 |
| 3529 | | |
| 3530 | | Missing or invalid "models" argument: invalid models specifier |
| 3531 | | |
| 3532 | | > rename #7 CS_P4_J219_sharp_flip id #6 |
| 3533 | | |
| 3534 | | > save CS_P4_J219_sharp_flip.mrc #6 |
| 3535 | | |
| 3536 | | > show #!3 models |
| 3537 | | |
| 3538 | | > select add #6 |
| 3539 | | |
| 3540 | | 2 models selected |
| 3541 | | |
| 3542 | | > view matrix models |
| 3543 | | > #6,0.55385,-0.83087,-0.054,184.1,0.82508,0.55638,-0.098423,-37.993,0.11182,0.009957,0.99368,-50.391 |
| 3544 | | |
| 3545 | | > ui mousemode right "translate selected models" |
| 3546 | | |
| 3547 | | > view matrix models |
| 3548 | | > #6,0.55385,-0.83087,-0.054,173.54,0.82508,0.55638,-0.098423,-43.189,0.11182,0.009957,0.99368,-15.893 |
| 3549 | | |
| 3550 | | > ui mousemode right "rotate selected models" |
| 3551 | | |
| 3552 | | > view matrix models |
| 3553 | | > #6,-0.43591,-0.88398,-0.16899,334.46,0.87276,-0.46105,0.1604,51.515,-0.2197,-0.077569,0.97248,45.011 |
| 3554 | | |
| 3555 | | > fitmap #6 inMap #3 |
| 3556 | | |
| 3557 | | Fit map CS_P4_J219_sharp_flip.mrc in map CS_P4_J216_class_2_sharp_flip.mrc |
| 3558 | | using 3507 points |
| 3559 | | correlation = 0.9414, correlation about mean = 0.4694, overlap = 2583 |
| 3560 | | steps = 72, shift = 3.53, angle = 4.05 degrees |
| 3561 | | |
| 3562 | | Position of CS_P4_J219_sharp_flip.mrc (#6) relative to |
| 3563 | | CS_P4_J216_class_2_sharp_flip.mrc (#3) coordinates: |
| 3564 | | Matrix rotation and translation |
| 3565 | | -0.45821390 -0.85833568 -0.23086770 342.48607306 |
| 3566 | | 0.85407817 -0.49711214 0.15306861 63.33623820 |
| 3567 | | -0.24615139 -0.12704090 0.96086946 56.91191433 |
| 3568 | | Axis -0.16142412 0.00880783 0.98684582 |
| 3569 | | Axis point 157.30567711 132.03749632 0.00000000 |
| 3570 | | Rotation angle (degrees) 119.81679395 |
| 3571 | | Shift along axis 1.43562700 |
| 3572 | | |
| 3573 | | |
| 3574 | | > select subtract #6 |
| 3575 | | |
| 3576 | | Nothing selected |
| 3577 | | |
| 3578 | | > hide #!3 models |
| 3579 | | |
| 3580 | | Unsupported scale factor (0.000000) detected on Display1 |
| 3581 | | |
| 3582 | | [Repeated 2 time(s)] |
| 3583 | | |
| 3584 | | > hide #!6 models |
| 3585 | | |
| 3586 | | > show #!3 models |
| 3587 | | |
| 3588 | | > show #!6 models |
| 3589 | | |
| 3590 | | > hide #!6 models |
| 3591 | | |
| 3592 | | > color #3 #B1DCFC |
| 3593 | | |
| 3594 | | > save P4_J216_class_2_sharp.png supersample 3 transparentBackground true |
| 3595 | | |
| 3596 | | > hide #!3 models |
| 3597 | | |
| 3598 | | > show #!3 models |
| 3599 | | |
| 3600 | | > hide #!3 models |
| 3601 | | |
| 3602 | | > show #!6 models |
| 3603 | | |
| 3604 | | > hide #!6 models |
| 3605 | | |
| 3606 | | > show #!3 models |
| 3607 | | |
| 3608 | | > show #!6 models |
| 3609 | | |
| 3610 | | > hide #!3 models |
| 3611 | | |
| 3612 | | > hide #!6 models |
| 3613 | | |
| 3614 | | > show #!3 models |
| 3615 | | |
| 3616 | | > color #3 #AFDBFF |
| 3617 | | |
| 3618 | | > hide #!3 models |
| 3619 | | |
| 3620 | | > show #!6 models |
| 3621 | | |
| 3622 | | > show #!3 models |
| 3623 | | |
| 3624 | | > hide #!3 models |
| 3625 | | |
| 3626 | | > show #!3 models |
| 3627 | | |
| 3628 | | > hide #!6 models |
| 3629 | | |
| 3630 | | > show #!6 models |
| 3631 | | |
| 3632 | | > color #3 #99BFE5 |
| 3633 | | |
| 3634 | | > hide #!6 models |
| 3635 | | |
| 3636 | | > view P1 |
| 3637 | | |
| 3638 | | > save P4_J216_class_2_sharp.png supersample 3 transparentBackground true |
| 3639 | | |
| 3640 | | > hide #!3 models |
| 3641 | | |
| 3642 | | > show #!6 models |
| 3643 | | |
| 3644 | | > ui mousemode right translate |
| 3645 | | |
| 3646 | | > save P4_J219_NU_sharp_flip.png supersample 3 transparentBackground true |
| 3647 | | |
| 3648 | | > save "/Users/peiyuyu/Documents/Goethe |
| 3649 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 3650 | | > F_B1/20260430_SA-F_B1_workflow.cxs" |
| 3651 | | |
| 3652 | | > open "/Users/peiyuyu/Documents/Goethe |
| 3653 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 3654 | | > F_B1/cryosparc_P4_J243_003_volume_map_sharp.mrc" |
| 3655 | | |
| 3656 | | Opened cryosparc_P4_J243_003_volume_map_sharp.mrc as #7, grid size 80,80,80, |
| 3657 | | pixel 3.32, shown at level 1.1, step 1, values float32 |
| 3658 | | |
| 3659 | | > select add #7 |
| 3660 | | |
| 3661 | | 2 models selected |
| 3662 | | |
| 3663 | | > ui mousemode right "rotate selected models" |
| 3664 | | |
| 3665 | | > view matrix models |
| 3666 | | > #7,0.34351,0.88804,0.30558,-69.154,0.93106,-0.36463,0.013018,61.425,0.12299,0.28005,-0.95208,168.53 |
| 3667 | | |
| 3668 | | > view matrix models |
| 3669 | | > #7,-0.72211,0.66266,0.19859,108.59,0.68148,0.73076,0.039546,-55.441,-0.11892,0.16389,-0.97928,217.85 |
| 3670 | | |
| 3671 | | > ui mousemode right "translate selected models" |
| 3672 | | |
| 3673 | | > view matrix models |
| 3674 | | > #7,-0.72211,0.66266,0.19859,107.8,0.68148,0.73076,0.039546,-63.119,-0.11892,0.16389,-0.97928,255.33 |
| 3675 | | |
| 3676 | | > view matrix models |
| 3677 | | > #7,-0.72211,0.66266,0.19859,116.79,0.68148,0.73076,0.039546,-56.849,-0.11892,0.16389,-0.97928,258.31 |
| 3678 | | |
| 3679 | | > fitmap #7 inMap #6 |
| 3680 | | |
| 3681 | | Fit map cryosparc_P4_J243_003_volume_map_sharp.mrc in map |
| 3682 | | CS_P4_J219_sharp_flip.mrc using 5116 points |
| 3683 | | correlation = 0.9377, correlation about mean = 0.7097, overlap = 1.519e+04 |
| 3684 | | steps = 76, shift = 2.16, angle = 10.6 degrees |
| 3685 | | |
| 3686 | | Position of cryosparc_P4_J243_003_volume_map_sharp.mrc (#7) relative to |
| 3687 | | CS_P4_J219_sharp_flip.mrc (#6) coordinates: |
| 3688 | | Matrix rotation and translation |
| 3689 | | 0.96909604 0.16642828 0.18208375 -39.18937125 |
| 3690 | | 0.15922720 -0.98578684 0.05358170 236.77616183 |
| 3691 | | 0.18841328 -0.02293313 -0.98182203 241.04145354 |
| 3692 | | Axis -0.99224103 -0.08208102 -0.09338329 |
| 3693 | | Axis point 0.00000000 122.34870623 120.06626952 |
| 3694 | | Rotation angle (degrees) 177.79032322 |
| 3695 | | Shift along axis -3.05877231 |
| 3696 | | |
| 3697 | | |
| 3698 | | > select subtract #7 |
| 3699 | | |
| 3700 | | Nothing selected |
| 3701 | | |
| 3702 | | > select add #7 |
| 3703 | | |
| 3704 | | 2 models selected |
| 3705 | | |
| 3706 | | > view matrix models |
| 3707 | | > #7,-0.62422,0.77517,0.097247,102.65,0.77737,0.62868,-0.021408,-52.876,-0.077732,0.062233,-0.99503,269.09 |
| 3708 | | |
| 3709 | | > ui mousemode right "rotate selected models" |
| 3710 | | |
| 3711 | | > view matrix models |
| 3712 | | > #7,-0.32669,-0.93485,0.13901,287.37,-0.94032,0.30667,-0.14745,222.88,0.095218,-0.17888,-0.97925,277.34 |
| 3713 | | |
| 3714 | | > ui mousemode right "translate selected models" |
| 3715 | | |
| 3716 | | > view matrix models |
| 3717 | | > #7,-0.32669,-0.93485,0.13901,281.61,-0.94032,0.30667,-0.14745,233.21,0.095218,-0.17888,-0.97925,273.65 |
| 3718 | | |
| 3719 | | > fitmap #7 inMap #6 |
| 3720 | | |
| 3721 | | Fit map cryosparc_P4_J243_003_volume_map_sharp.mrc in map |
| 3722 | | CS_P4_J219_sharp_flip.mrc using 5116 points |
| 3723 | | correlation = 0.9419, correlation about mean = 0.7222, overlap = 1.551e+04 |
| 3724 | | steps = 56, shift = 3.52, angle = 7.25 degrees |
| 3725 | | |
| 3726 | | Position of cryosparc_P4_J243_003_volume_map_sharp.mrc (#7) relative to |
| 3727 | | CS_P4_J219_sharp_flip.mrc (#6) coordinates: |
| 3728 | | Matrix rotation and translation |
| 3729 | | -0.62645631 0.77281267 0.10155321 101.66723571 |
| 3730 | | 0.77509514 0.63140454 -0.02357570 -50.90352474 |
| 3731 | | -0.08234076 0.06394425 -0.99455072 266.97848709 |
| 3732 | | Axis 0.42971205 0.90289643 0.01120665 |
| 3733 | | Axis point 70.49413903 0.00000000 130.93970837 |
| 3734 | | Rotation angle (degrees) 174.15511884 |
| 3735 | | Shift along axis 0.71895896 |
| 3736 | | |
| 3737 | | |
| 3738 | | > select subtract #7 |
| 3739 | | |
| 3740 | | Nothing selected |
| 3741 | | |
| 3742 | | > hide #!6 models |
| 3743 | | |
| 3744 | | > volume #7 level 1.41 |
| 3745 | | |
| 3746 | | > color #7 #99BFE5 |
| 3747 | | |
| 3748 | | > view P1 |
| 3749 | | |
| 3750 | | > close #7 |
| 3751 | | |
| 3752 | | > open "/Users/peiyuyu/Documents/Goethe |
| 3753 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 3754 | | > F_B1/cryosparc_P4_J244_003_volume_map_sharp.mrc" |
| 3755 | | |
| 3756 | | Opened cryosparc_P4_J244_003_volume_map_sharp.mrc as #7, grid size 80,80,80, |
| 3757 | | pixel 3.32, shown at level 1.05, step 1, values float32 |
| 3758 | | |
| 3759 | | > show #!6 models |
| 3760 | | |
| 3761 | | > select add #7 |
| 3762 | | |
| 3763 | | 2 models selected |
| 3764 | | |
| 3765 | | > ui mousemode right "rotate selected models" |
| 3766 | | |
| 3767 | | > view matrix models |
| 3768 | | > #7,0.71966,-0.6805,-0.13788,149.49,-0.69285,-0.71678,-0.078638,342.79,-0.045318,0.15212,-0.98732,223.99 |
| 3769 | | |
| 3770 | | > hide #!6 models |
| 3771 | | |
| 3772 | | > show #!6 models |
| 3773 | | |
| 3774 | | > ui mousemode right "translate selected models" |
| 3775 | | |
| 3776 | | > view matrix models |
| 3777 | | > #7,0.71966,-0.6805,-0.13788,149.4,-0.69285,-0.71678,-0.078638,338.98,-0.045318,0.15212,-0.98732,258.19 |
| 3778 | | |
| 3779 | | > select subtract #7 |
| 3780 | | |
| 3781 | | Nothing selected |
| 3782 | | |
| 3783 | | > hide #!6 models |
| 3784 | | |
| 3785 | | > volume flip #7 |
| 3786 | | |
| 3787 | | Opened cryosparc_P4_J244_003_volume_map_sharp.mrc z flip as #8, grid size |
| 3788 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 3789 | | |
| 3790 | | > close #7 |
| 3791 | | |
| 3792 | | > rename #8 CS_P4_J244_sharp_flip id #7 |
| 3793 | | |
| 3794 | | > save CS_P4_J244_sharp_flip.mrc #7 |
| 3795 | | |
| 3796 | | > color #7 #99BFE5 |
| 3797 | | |
| 3798 | | > volume #7 level 1.156 |
| 3799 | | |
| 3800 | | > save CS_P4_J244_sharp_flip.mrc #7 |
| 3801 | | |
| 3802 | | > save P4_J244_NU_sharp_flip.png supersample 3 transparentBackground true |
| 3803 | | |
| 3804 | | > save "/Users/peiyuyu/Documents/Goethe |
| 3805 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 3806 | | > F_B1/20260501_SA-F_B1_workflow.cxs" |
| 3807 | | |
| 3808 | | > open "/Users/peiyuyu/Documents/Goethe |
| 3809 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 3810 | | > F_B1/cryosparc_P4_J242_class_00_00122_volume_sharp.mrc" |
| 3811 | | |
| 3812 | | Opened cryosparc_P4_J242_class_00_00122_volume_sharp.mrc as #8, grid size |
| 3813 | | 128,128,128, pixel 2.08, shown at level 0.192, step 1, values float32 |
| 3814 | | |
| 3815 | | > hide #!7 models |
| 3816 | | |
| 3817 | | > volume #8 level 0.2208 |
| 3818 | | |
| 3819 | | > show #!7 models |
| 3820 | | |
| 3821 | | > select add #8 |
| 3822 | | |
| 3823 | | 2 models selected |
| 3824 | | |
| 3825 | | > view matrix models #8,1,0,0,1.7912,0,1,0,4.218,0,0,1,1.8536 |
| 3826 | | |
| 3827 | | > ui mousemode right "rotate selected models" |
| 3828 | | |
| 3829 | | > view matrix models |
| 3830 | | > #8,0.019063,-0.87425,-0.4851,301.31,0.055421,0.48537,-0.87255,194.56,0.99828,-0.010251,0.057705,27.748 |
| 3831 | | |
| 3832 | | > ui mousemode right "translate selected models" |
| 3833 | | |
| 3834 | | > view matrix models |
| 3835 | | > #8,0.019063,-0.87425,-0.4851,303.09,0.055421,0.48537,-0.87255,199.03,0.99828,-0.010251,0.057705,19.845 |
| 3836 | | |
| 3837 | | > view matrix models |
| 3838 | | > #8,0.019063,-0.87425,-0.4851,323.73,0.055421,0.48537,-0.87255,193.31,0.99828,-0.010251,0.057705,17.439 |
| 3839 | | |
| 3840 | | > view matrix models |
| 3841 | | > #8,0.019063,-0.87425,-0.4851,322.89,0.055421,0.48537,-0.87255,188.18,0.99828,-0.010251,0.057705,19.28 |
| 3842 | | |
| 3843 | | > fitmap #8 inMap #7 |
| 3844 | | |
| 3845 | | Fit map cryosparc_P4_J242_class_00_00122_volume_sharp.mrc in map |
| 3846 | | CS_P4_J244_sharp_flip.mrc using 14754 points |
| 3847 | | correlation = 0.481, correlation about mean = 0.07756, overlap = 1756 |
| 3848 | | steps = 112, shift = 13.3, angle = 15.3 degrees |
| 3849 | | |
| 3850 | | Position of cryosparc_P4_J242_class_00_00122_volume_sharp.mrc (#8) relative to |
| 3851 | | CS_P4_J244_sharp_flip.mrc (#7) coordinates: |
| 3852 | | Matrix rotation and translation |
| 3853 | | 0.05423844 -0.87969653 0.47243223 170.87663448 |
| 3854 | | 0.10002936 0.47553549 0.87399092 -59.90285646 |
| 3855 | | -0.99350507 -0.00014682 0.11378778 232.31099922 |
| 3856 | | Axis -0.44417982 0.74489379 0.49783284 |
| 3857 | | Axis point 199.16697689 -0.00000000 41.59458970 |
| 3858 | | Rotation angle (degrees) 100.26604714 |
| 3859 | | Shift along axis -4.86917491 |
| 3860 | | |
| 3861 | | |
| 3862 | | > select subtract #8 |
| 3863 | | |
| 3864 | | Nothing selected |
| 3865 | | |
| 3866 | | > hide #!7 models |
| 3867 | | |
| 3868 | | > open "/Users/peiyuyu/Documents/Goethe |
| 3869 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 3870 | | > F_B1/cryosparc_P4_J242_class_01_00122_volume_sharp.mrc" |
| 3871 | | |
| 3872 | | Opened cryosparc_P4_J242_class_01_00122_volume_sharp.mrc as #9, grid size |
| 3873 | | 128,128,128, pixel 2.08, shown at level 0.178, step 1, values float32 |
| 3874 | | |
| 3875 | | > hide #!8 models |
| 3876 | | |
| 3877 | | > volume #9 level 0.1464 |
| 3878 | | |
| 3879 | | > show #!8 models |
| 3880 | | |
| 3881 | | > select add #9 |
| 3882 | | |
| 3883 | | 2 models selected |
| 3884 | | |
| 3885 | | > ui mousemode right "rotate selected models" |
| 3886 | | |
| 3887 | | > view matrix models |
| 3888 | | > #9,0.01099,0.99675,0.079835,4.9565,0.1314,-0.080587,0.98805,-14.68,0.99127,-0.00036851,-0.13186,9.8 |
| 3889 | | |
| 3890 | | > ui mousemode right "translate selected models" |
| 3891 | | |
| 3892 | | > view matrix models |
| 3893 | | > #9,0.01099,0.99675,0.079835,10.903,0.1314,-0.080587,0.98805,5.6709,0.99127,-0.00036851,-0.13186,51.786 |
| 3894 | | |
| 3895 | | > view matrix models |
| 3896 | | > #9,0.01099,0.99675,0.079835,4.7224,0.1314,-0.080587,0.98805,-1.1935,0.99127,-0.00036851,-0.13186,45.788 |
| 3897 | | |
| 3898 | | > fitmap #9 inMap #8 |
| 3899 | | |
| 3900 | | Fit map cryosparc_P4_J242_class_01_00122_volume_sharp.mrc in map |
| 3901 | | cryosparc_P4_J242_class_00_00122_volume_sharp.mrc using 29766 points |
| 3902 | | correlation = 0.8496, correlation about mean = 0.3082, overlap = 1328 |
| 3903 | | steps = 284, shift = 18.3, angle = 53.9 degrees |
| 3904 | | |
| 3905 | | Position of cryosparc_P4_J242_class_01_00122_volume_sharp.mrc (#9) relative to |
| 3906 | | cryosparc_P4_J242_class_00_00122_volume_sharp.mrc (#8) coordinates: |
| 3907 | | Matrix rotation and translation |
| 3908 | | 0.99575722 -0.02668322 0.08806571 -8.52134797 |
| 3909 | | -0.09034177 -0.46538978 0.88048323 89.22150736 |
| 3910 | | 0.01749075 -0.88470354 -0.46582584 307.69453028 |
| 3911 | | Axis -0.99855360 0.03992375 -0.03601119 |
| 3912 | | Axis point 0.00000000 137.23273941 127.10917488 |
| 3913 | | Rotation angle (degrees) 117.88699495 |
| 3914 | | Shift along axis 0.99063260 |
| 3915 | | |
| 3916 | | |
| 3917 | | > select subtract #9 |
| 3918 | | |
| 3919 | | Nothing selected |
| 3920 | | |
| 3921 | | > hide #!8 models |
| 3922 | | |
| 3923 | | > hide #!9 models |
| 3924 | | |
| 3925 | | > open "/Users/peiyuyu/Documents/Goethe |
| 3926 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 3927 | | > F_B1/cryosparc_P4_J242_class_02_00122_volume_sharp.mrc" |
| 3928 | | |
| 3929 | | Opened cryosparc_P4_J242_class_02_00122_volume_sharp.mrc as #10, grid size |
| 3930 | | 128,128,128, pixel 2.08, shown at level 0.195, step 1, values float32 |
| 3931 | | |
| 3932 | | > select add #10 |
| 3933 | | |
| 3934 | | 2 models selected |
| 3935 | | |
| 3936 | | > show #!9 models |
| 3937 | | |
| 3938 | | > ui mousemode right "rotate selected models" |
| 3939 | | |
| 3940 | | > view matrix models |
| 3941 | | > #10,-0.17462,0.93232,0.31669,19.869,0.21917,0.35036,-0.91061,163.76,-0.95993,-0.089597,-0.26552,316.03 |
| 3942 | | |
| 3943 | | > ui mousemode right "translate selected models" |
| 3944 | | |
| 3945 | | > view matrix models |
| 3946 | | > #10,-0.17462,0.93232,0.31669,8.7975,0.21917,0.35036,-0.91061,193.69,-0.95993,-0.089597,-0.26552,324.3 |
| 3947 | | |
| 3948 | | > view matrix models |
| 3949 | | > #10,-0.17462,0.93232,0.31669,5.0809,0.21917,0.35036,-0.91061,186.44,-0.95993,-0.089597,-0.26552,324.52 |
| 3950 | | |
| 3951 | | > fitmap #10 inMap #9 |
| 3952 | | |
| 3953 | | Fit map cryosparc_P4_J242_class_02_00122_volume_sharp.mrc in map |
| 3954 | | cryosparc_P4_J242_class_01_00122_volume_sharp.mrc using 20970 points |
| 3955 | | correlation = 0.8182, correlation about mean = 0.3079, overlap = 1048 |
| 3956 | | steps = 212, shift = 18, angle = 29.3 degrees |
| 3957 | | |
| 3958 | | Position of cryosparc_P4_J242_class_02_00122_volume_sharp.mrc (#10) relative |
| 3959 | | to cryosparc_P4_J242_class_01_00122_volume_sharp.mrc (#9) coordinates: |
| 3960 | | Matrix rotation and translation |
| 3961 | | -0.92160797 -0.37293352 0.10751436 286.70013052 |
| 3962 | | -0.37621404 0.79028143 -0.48365097 141.61699812 |
| 3963 | | 0.09540306 -0.48618500 -0.86863249 299.79876520 |
| 3964 | | Axis -0.19795503 0.94611818 -0.25626978 |
| 3965 | | Axis point 158.67244900 0.00000000 168.59386356 |
| 3966 | | Rotation angle (degrees) 179.63327476 |
| 3967 | | Shift along axis 0.40332117 |
| 3968 | | |
| 3969 | | |
| 3970 | | > select subtract #10 |
| 3971 | | |
| 3972 | | Nothing selected |
| 3973 | | |
| 3974 | | > hide #!9 models |
| 3975 | | |
| 3976 | | > hide #!10 models |
| 3977 | | |
| 3978 | | > open "/Users/peiyuyu/Documents/Goethe |
| 3979 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 3980 | | > F_B1/cryosparc_P4_J242_class_03_00122_volume_sharp.mrc" |
| 3981 | | |
| 3982 | | Opened cryosparc_P4_J242_class_03_00122_volume_sharp.mrc as #11, grid size |
| 3983 | | 128,128,128, pixel 2.08, shown at level 0.218, step 1, values float32 |
| 3984 | | |
| 3985 | | > volume #11 level 0.1502 |
| 3986 | | |
| 3987 | | > show #!10 models |
| 3988 | | |
| 3989 | | > select add #11 |
| 3990 | | |
| 3991 | | 2 models selected |
| 3992 | | |
| 3993 | | > ui mousemode right "rotate selected models" |
| 3994 | | |
| 3995 | | > view matrix models |
| 3996 | | > #11,0.73904,-0.47164,0.48101,26.135,-0.11204,0.61802,0.77814,-47.481,-0.66427,-0.62897,0.4039,253.49 |
| 3997 | | |
| 3998 | | > ui mousemode right "translate selected models" |
| 3999 | | |
| 4000 | | > view matrix models |
| 4001 | | > #11,0.73904,-0.47164,0.48101,5.5926,-0.11204,0.61802,0.77814,-27.642,-0.66427,-0.62897,0.4039,266.06 |
| 4002 | | |
| 4003 | | > ui mousemode right "rotate selected models" |
| 4004 | | |
| 4005 | | > view matrix models |
| 4006 | | > #11,-0.56676,0.0073102,0.82385,78.335,0.19153,-0.97139,0.14038,216.5,0.80131,0.23736,0.54915,-63.542 |
| 4007 | | |
| 4008 | | > select subtract #11 |
| 4009 | | |
| 4010 | | Nothing selected |
| 4011 | | |
| 4012 | | > fitmap #11 inMap #10 |
| 4013 | | |
| 4014 | | Fit map cryosparc_P4_J242_class_03_00122_volume_sharp.mrc in map |
| 4015 | | cryosparc_P4_J242_class_02_00122_volume_sharp.mrc using 45754 points |
| 4016 | | correlation = 0.7333, correlation about mean = -0.04196, overlap = 1268 |
| 4017 | | steps = 132, shift = 23.8, angle = 15.3 degrees |
| 4018 | | |
| 4019 | | Position of cryosparc_P4_J242_class_03_00122_volume_sharp.mrc (#11) relative |
| 4020 | | to cryosparc_P4_J242_class_02_00122_volume_sharp.mrc (#10) coordinates: |
| 4021 | | Matrix rotation and translation |
| 4022 | | -0.26691235 0.13508665 -0.95420616 300.80664915 |
| 4023 | | -0.90776913 -0.36770197 0.20186745 274.07355373 |
| 4024 | | -0.32359389 0.92007981 0.22077169 18.93443182 |
| 4025 | | Axis 0.50771970 -0.44579332 -0.73721708 |
| 4026 | | Axis point 236.02557416 49.14154001 0.00000000 |
| 4027 | | Rotation angle (degrees) 134.98497404 |
| 4028 | | Shift along axis 16.58651491 |
| 4029 | | |
| 4030 | | |
| 4031 | | > hide #!10 models |
| 4032 | | |
| 4033 | | > open "/Users/peiyuyu/Documents/Goethe |
| 4034 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 4035 | | > F_B1/cryosparc_P4_J242_class_04_00122_volume_sharp.mrc" |
| 4036 | | |
| 4037 | | Opened cryosparc_P4_J242_class_04_00122_volume_sharp.mrc as #12, grid size |
| 4038 | | 128,128,128, pixel 2.08, shown at level 0.261, step 1, values float32 |
| 4039 | | |
| 4040 | | > show #!10 models |
| 4041 | | |
| 4042 | | > hide #!10 models |
| 4043 | | |
| 4044 | | > select add #12 |
| 4045 | | |
| 4046 | | 2 models selected |
| 4047 | | |
| 4048 | | > ui mousemode right "translate selected models" |
| 4049 | | |
| 4050 | | > view matrix models #12,1,0,0,-3.7479,0,1,0,3.9168,0,0,1,20.991 |
| 4051 | | |
| 4052 | | > ui mousemode right "rotate selected models" |
| 4053 | | |
| 4054 | | > view matrix models |
| 4055 | | > #12,0.79132,-0.13023,0.59737,-27.645,0.22144,0.97176,-0.081485,-12.263,-0.56989,0.19676,0.79781,93.603 |
| 4056 | | |
| 4057 | | > ui mousemode right "translate selected models" |
| 4058 | | |
| 4059 | | > view matrix models |
| 4060 | | > #12,0.79132,-0.13023,0.59737,-50.124,0.22144,0.97176,-0.081485,3.5285,-0.56989,0.19676,0.79781,102.68 |
| 4061 | | |
| 4062 | | > view matrix models |
| 4063 | | > #12,0.79132,-0.13023,0.59737,-48.335,0.22144,0.97176,-0.081485,2.277,-0.56989,0.19676,0.79781,101.98 |
| 4064 | | |
| 4065 | | > view matrix models |
| 4066 | | > #12,0.79132,-0.13023,0.59737,-41.566,0.22144,0.97176,-0.081485,-1.9637,-0.56989,0.19676,0.79781,101.41 |
| 4067 | | |
| 4068 | | > fitmap #12 inMap #11 |
| 4069 | | |
| 4070 | | Fit map cryosparc_P4_J242_class_04_00122_volume_sharp.mrc in map |
| 4071 | | cryosparc_P4_J242_class_03_00122_volume_sharp.mrc using 20956 points |
| 4072 | | correlation = 0.8514, correlation about mean = 0.04285, overlap = 1430 |
| 4073 | | steps = 80, shift = 7.16, angle = 11.7 degrees |
| 4074 | | |
| 4075 | | Position of cryosparc_P4_J242_class_04_00122_volume_sharp.mrc (#12) relative |
| 4076 | | to cryosparc_P4_J242_class_03_00122_volume_sharp.mrc (#11) coordinates: |
| 4077 | | Matrix rotation and translation |
| 4078 | | -0.93263188 0.26201641 0.24808299 197.07280134 |
| 4079 | | -0.29117819 -0.95255879 -0.08858337 308.05329962 |
| 4080 | | 0.21310334 -0.15485204 0.96468016 17.95641726 |
| 4081 | | Axis -0.11870856 0.06265985 -0.99095006 |
| 4082 | | Axis point 119.69175871 140.62215054 0.00000000 |
| 4083 | | Rotation angle (degrees) 163.79210928 |
| 4084 | | Shift along axis -21.88556717 |
| 4085 | | |
| 4086 | | |
| 4087 | | > select subtract #12 |
| 4088 | | |
| 4089 | | Nothing selected |
| 4090 | | |
| 4091 | | > hide #!11 models |
| 4092 | | |
| 4093 | | > volume #12 level 0.4033 |
| 4094 | | |
| 4095 | | > volume #12 level 0.3453 |
| 4096 | | |
| 4097 | | > color #8 #FCEDFF |
| 4098 | | |
| 4099 | | > hide #!12 models |
| 4100 | | |
| 4101 | | > show #!8 models |
| 4102 | | |
| 4103 | | > hide #!8 models |
| 4104 | | |
| 4105 | | > show #!9 models |
| 4106 | | |
| 4107 | | > color #9 #FCEDFF |
| 4108 | | |
| 4109 | | > hide #!9 models |
| 4110 | | |
| 4111 | | > show #!10 models |
| 4112 | | |
| 4113 | | > color #10 #FCEDFF |
| 4114 | | |
| 4115 | | > hide #!10 models |
| 4116 | | |
| 4117 | | > show #!11 models |
| 4118 | | |
| 4119 | | > color #11 #FCEDFF |
| 4120 | | |
| 4121 | | > hide #!11 models |
| 4122 | | |
| 4123 | | > show #!12 models |
| 4124 | | |
| 4125 | | > color #12 #99BFE5 |
| 4126 | | |
| 4127 | | > view P1 |
| 4128 | | |
| 4129 | | > show #!2 models |
| 4130 | | |
| 4131 | | > hide #!2 models |
| 4132 | | |
| 4133 | | > show #!3 models |
| 4134 | | |
| 4135 | | > hide #!3 models |
| 4136 | | |
| 4137 | | > show #!4 models |
| 4138 | | |
| 4139 | | > hide #!4 models |
| 4140 | | |
| 4141 | | > show #!1 models |
| 4142 | | |
| 4143 | | > hide #!1 models |
| 4144 | | |
| 4145 | | > show #!6 models |
| 4146 | | |
| 4147 | | > hide #!6 models |
| 4148 | | |
| 4149 | | > show #!3 models |
| 4150 | | |
| 4151 | | > select add #12 |
| 4152 | | |
| 4153 | | 2 models selected |
| 4154 | | |
| 4155 | | > ui mousemode right "rotate selected models" |
| 4156 | | |
| 4157 | | > view matrix models |
| 4158 | | > #12,0.95369,-0.29732,-0.045496,35.025,0.29889,0.95373,0.032695,-17.906,0.03367,-0.044779,0.99843,26.075 |
| 4159 | | |
| 4160 | | > view matrix models |
| 4161 | | > #12,-0.8879,0.32078,-0.32974,233.6,0.30063,0.94715,0.1119,-26.299,0.34821,0.00022498,-0.93742,198.42 |
| 4162 | | |
| 4163 | | > ui mousemode right "translate selected models" |
| 4164 | | |
| 4165 | | > view matrix models |
| 4166 | | > #12,-0.8879,0.32078,-0.32974,232.89,0.30063,0.94715,0.1119,-34.317,0.34821,0.00022498,-0.93742,213.72 |
| 4167 | | |
| 4168 | | > view matrix models |
| 4169 | | > #12,-0.8879,0.32078,-0.32974,243.25,0.30063,0.94715,0.1119,-55.273,0.34821,0.00022498,-0.93742,214.76 |
| 4170 | | |
| 4171 | | > ui mousemode right "rotate selected models" |
| 4172 | | |
| 4173 | | > view matrix models |
| 4174 | | > #12,-0.88731,0.39919,0.23093,169.34,0.40717,0.91324,-0.014156,-50.796,-0.21654,0.081466,-0.97287,283.71 |
| 4175 | | |
| 4176 | | > fitmap #12 inMap #3 |
| 4177 | | |
| 4178 | | Fit map cryosparc_P4_J242_class_04_00122_volume_sharp.mrc in map |
| 4179 | | CS_P4_J216_class_2_sharp_flip.mrc using 12546 points |
| 4180 | | correlation = 0.9893, correlation about mean = 0.7632, overlap = 2433 |
| 4181 | | steps = 124, shift = 11, angle = 27.9 degrees |
| 4182 | | |
| 4183 | | Position of cryosparc_P4_J242_class_04_00122_volume_sharp.mrc (#12) relative |
| 4184 | | to CS_P4_J216_class_2_sharp_flip.mrc (#3) coordinates: |
| 4185 | | Matrix rotation and translation |
| 4186 | | -0.62359754 0.77535022 0.09979057 101.14085164 |
| 4187 | | 0.77719680 0.62864768 -0.02769904 -50.42984630 |
| 4188 | | -0.08420957 0.06028386 -0.99462285 268.88184712 |
| 4189 | | Axis 0.43136931 0.90213001 0.00905357 |
| 4190 | | Axis point 70.24601107 0.00000000 131.83510298 |
| 4191 | | Rotation angle (degrees) 174.14674629 |
| 4192 | | Shift along axis 0.56912319 |
| 4193 | | |
| 4194 | | |
| 4195 | | > select subtract #12 |
| 4196 | | |
| 4197 | | Nothing selected |
| 4198 | | |
| 4199 | | > hide #!12 models |
| 4200 | | |
| 4201 | | > show #!12 models |
| 4202 | | |
| 4203 | | > show #!11 models |
| 4204 | | |
| 4205 | | > select add #11 |
| 4206 | | |
| 4207 | | 2 models selected |
| 4208 | | |
| 4209 | | > view matrix models |
| 4210 | | > #11,-0.37852,0.89247,0.24538,28.817,0.88041,0.42898,-0.20213,16.101,-0.28566,0.13952,-0.94812,300.03 |
| 4211 | | |
| 4212 | | > ui mousemode right "translate selected models" |
| 4213 | | |
| 4214 | | > view matrix models |
| 4215 | | > #11,-0.37852,0.89247,0.24538,46.8,0.88041,0.42898,-0.20213,-5.6117,-0.28566,0.13952,-0.94812,300.85 |
| 4216 | | |
| 4217 | | > view matrix models |
| 4218 | | > #11,-0.37852,0.89247,0.24538,36.63,0.88041,0.42898,-0.20213,-16.953,-0.28566,0.13952,-0.94812,296.6 |
| 4219 | | |
| 4220 | | > fitmap #11 inMap #12 |
| 4221 | | |
| 4222 | | Fit map cryosparc_P4_J242_class_03_00122_volume_sharp.mrc in map |
| 4223 | | cryosparc_P4_J242_class_04_00122_volume_sharp.mrc using 45754 points |
| 4224 | | correlation = 0.7192, correlation about mean = 0.1299, overlap = 1714 |
| 4225 | | steps = 96, shift = 6.98, angle = 12.7 degrees |
| 4226 | | |
| 4227 | | Position of cryosparc_P4_J242_class_03_00122_volume_sharp.mrc (#11) relative |
| 4228 | | to cryosparc_P4_J242_class_04_00122_volume_sharp.mrc (#12) coordinates: |
| 4229 | | Matrix rotation and translation |
| 4230 | | 0.88557701 -0.25567312 -0.38779454 99.27082409 |
| 4231 | | 0.32552043 0.93717068 0.12548928 -53.44589150 |
| 4232 | | 0.33134544 -0.23736546 0.91316364 -22.95393091 |
| 4233 | | Axis -0.36530839 -0.72400286 0.58512361 |
| 4234 | | Axis point 106.02252826 0.00000000 214.34257569 |
| 4235 | | Rotation angle (degrees) 29.77806268 |
| 4236 | | Shift along axis -11.00037360 |
| 4237 | | |
| 4238 | | |
| 4239 | | > select subtract #11 |
| 4240 | | |
| 4241 | | Nothing selected |
| 4242 | | |
| 4243 | | > hide #!12 models |
| 4244 | | |
| 4245 | | > show #!12 models |
| 4246 | | |
| 4247 | | > hide #!12 models |
| 4248 | | |
| 4249 | | > hide #!3 models |
| 4250 | | |
| 4251 | | > show #!12 models |
| 4252 | | |
| 4253 | | > hide #!11 models |
| 4254 | | |
| 4255 | | > show #!10 models |
| 4256 | | |
| 4257 | | > select add #10 |
| 4258 | | |
| 4259 | | 2 models selected |
| 4260 | | |
| 4261 | | > view matrix models |
| 4262 | | > #10,-0.49822,0.81176,0.30466,37.524,-0.14214,0.27015,-0.95227,247.33,-0.85532,-0.51775,-0.019207,343.36 |
| 4263 | | |
| 4264 | | > ui mousemode right "rotate selected models" |
| 4265 | | |
| 4266 | | > view matrix models |
| 4267 | | > #10,-0.013344,0.85199,0.52339,-68.961,-0.1627,-0.51831,0.83957,128.11,0.98659,-0.073951,0.14553,-15.381 |
| 4268 | | |
| 4269 | | > ui mousemode right "translate selected models" |
| 4270 | | |
| 4271 | | > view matrix models |
| 4272 | | > #10,-0.013344,0.85199,0.52339,-45.161,-0.1627,-0.51831,0.83957,119.13,0.98659,-0.073951,0.14553,-13.578 |
| 4273 | | |
| 4274 | | > fitmap #10 inMap #12 |
| 4275 | | |
| 4276 | | Fit map cryosparc_P4_J242_class_02_00122_volume_sharp.mrc in map |
| 4277 | | cryosparc_P4_J242_class_04_00122_volume_sharp.mrc using 20970 points |
| 4278 | | correlation = 0.7101, correlation about mean = 0.02358, overlap = 908.6 |
| 4279 | | steps = 80, shift = 7.98, angle = 9.33 degrees |
| 4280 | | |
| 4281 | | Position of cryosparc_P4_J242_class_02_00122_volume_sharp.mrc (#10) relative |
| 4282 | | to cryosparc_P4_J242_class_04_00122_volume_sharp.mrc (#12) coordinates: |
| 4283 | | Matrix rotation and translation |
| 4284 | | -0.35385865 -0.88893073 0.29083707 267.80089120 |
| 4285 | | -0.02210060 0.31881642 0.94755878 -31.84809054 |
| 4286 | | -0.93503776 0.32887420 -0.13246184 232.75133151 |
| 4287 | | Axis -0.38099533 0.75491227 0.53380711 |
| 4288 | | Axis point 205.22642342 0.00000000 36.94010088 |
| 4289 | | Rotation angle (degrees) 125.71487620 |
| 4290 | | Shift along axis -1.82908632 |
| 4291 | | |
| 4292 | | |
| 4293 | | > ui mousemode right "rotate selected models" |
| 4294 | | |
| 4295 | | > view matrix models |
| 4296 | | > #10,-0.12287,-0.47662,0.87048,95.543,0.19038,-0.87215,-0.45067,269.26,0.97399,0.11035,0.1979,-37.789 |
| 4297 | | |
| 4298 | | > select subtract #10 |
| 4299 | | |
| 4300 | | Nothing selected |
| 4301 | | |
| 4302 | | > hide #!12 models |
| 4303 | | |
| 4304 | | > show #!11 models |
| 4305 | | |
| 4306 | | > show #!12 models |
| 4307 | | |
| 4308 | | > hide #!10 models |
| 4309 | | |
| 4310 | | > hide #!12 models |
| 4311 | | |
| 4312 | | > show #!10 models |
| 4313 | | |
| 4314 | | > select add #10 |
| 4315 | | |
| 4316 | | 2 models selected |
| 4317 | | |
| 4318 | | > view matrix models |
| 4319 | | > #10,-0.38685,-0.21971,0.89558,99.669,-0.053858,-0.96416,-0.2598,294.54,0.92057,-0.14874,0.36115,-16.709 |
| 4320 | | |
| 4321 | | > fitmap #10 inMap #11 |
| 4322 | | |
| 4323 | | Fit map cryosparc_P4_J242_class_02_00122_volume_sharp.mrc in map |
| 4324 | | cryosparc_P4_J242_class_03_00122_volume_sharp.mrc using 20970 points |
| 4325 | | correlation = 0.8482, correlation about mean = 0.1221, overlap = 870.1 |
| 4326 | | steps = 88, shift = 3.31, angle = 12.5 degrees |
| 4327 | | |
| 4328 | | Position of cryosparc_P4_J242_class_02_00122_volume_sharp.mrc (#10) relative |
| 4329 | | to cryosparc_P4_J242_class_03_00122_volume_sharp.mrc (#11) coordinates: |
| 4330 | | Matrix rotation and translation |
| 4331 | | -0.32110732 -0.67353914 -0.66575904 357.49560201 |
| 4332 | | 0.09667773 -0.72262784 0.68444314 122.97291760 |
| 4333 | | -0.94209527 0.15541563 0.29715733 219.46626263 |
| 4334 | | Axis -0.54292343 0.28359472 0.79044809 |
| 4335 | | Axis point 234.55310205 82.20310947 0.00000000 |
| 4336 | | Rotation angle (degrees) 150.84311237 |
| 4337 | | Shift along axis 14.25842210 |
| 4338 | | |
| 4339 | | |
| 4340 | | > view matrix models |
| 4341 | | > #10,-0.30509,0.38504,-0.87101,233.77,0.18733,0.92101,0.34153,-54.422,0.93372,-0.058973,-0.35312,61.617 |
| 4342 | | |
| 4343 | | > ui mousemode right "translate selected models" |
| 4344 | | |
| 4345 | | > view matrix models |
| 4346 | | > #10,-0.30509,0.38504,-0.87101,240.35,0.18733,0.92101,0.34153,-58.712,0.93372,-0.058973,-0.35312,49.559 |
| 4347 | | |
| 4348 | | > view matrix models |
| 4349 | | > #10,-0.30509,0.38504,-0.87101,238.16,0.18733,0.92101,0.34153,-58.03,0.93372,-0.058973,-0.35312,51.895 |
| 4350 | | |
| 4351 | | > ui mousemode right "rotate selected models" |
| 4352 | | |
| 4353 | | > view matrix models |
| 4354 | | > #10,-0.36524,0.52001,-0.77213,217.65,0.08187,0.84416,0.5298,-55.56,0.9273,0.13029,-0.3509,28.264 |
| 4355 | | |
| 4356 | | > fitmap #10 inMap #11 |
| 4357 | | |
| 4358 | | Fit map cryosparc_P4_J242_class_02_00122_volume_sharp.mrc in map |
| 4359 | | cryosparc_P4_J242_class_03_00122_volume_sharp.mrc using 20970 points |
| 4360 | | correlation = 0.8485, correlation about mean = 0.1929, overlap = 854.7 |
| 4361 | | steps = 140, shift = 18.2, angle = 22.5 degrees |
| 4362 | | |
| 4363 | | Position of cryosparc_P4_J242_class_02_00122_volume_sharp.mrc (#10) relative |
| 4364 | | to cryosparc_P4_J242_class_03_00122_volume_sharp.mrc (#11) coordinates: |
| 4365 | | Matrix rotation and translation |
| 4366 | | -0.33967352 0.22689098 0.91276633 25.87088295 |
| 4367 | | 0.00914596 0.97121791 -0.23801704 28.63290177 |
| 4368 | | -0.94049893 -0.07249996 -0.33197214 335.97765353 |
| 4369 | | Axis 0.08835402 0.98928424 -0.11623361 |
| 4370 | | Axis point 129.11230783 0.00000000 160.33867701 |
| 4371 | | Rotation angle (degrees) 110.50039702 |
| 4372 | | Shift along axis -8.44002041 |
| 4373 | | |
| 4374 | | |
| 4375 | | > view matrix models |
| 4376 | | > #10,-0.23006,0.48061,-0.84622,212.13,0.22993,0.87177,0.43261,-89.291,0.94562,-0.095042,-0.31107,58.162 |
| 4377 | | |
| 4378 | | > ui mousemode right "translate selected models" |
| 4379 | | |
| 4380 | | > view matrix models |
| 4381 | | > #10,-0.23006,0.48061,-0.84622,206.51,0.22993,0.87177,0.43261,-81.849,0.94562,-0.095042,-0.31107,43.385 |
| 4382 | | |
| 4383 | | > view matrix models |
| 4384 | | > #10,-0.23006,0.48061,-0.84622,209.88,0.22993,0.87177,0.43261,-83.853,0.94562,-0.095042,-0.31107,44.292 |
| 4385 | | |
| 4386 | | > select subtract #10 |
| 4387 | | |
| 4388 | | Nothing selected |
| 4389 | | |
| 4390 | | > hide #!10 models |
| 4391 | | |
| 4392 | | > hide #!11 models |
| 4393 | | |
| 4394 | | > show #!10 models |
| 4395 | | |
| 4396 | | > hide #!10 models |
| 4397 | | |
| 4398 | | > show #!9 models |
| 4399 | | |
| 4400 | | > show #!10 models |
| 4401 | | |
| 4402 | | > select add #9 |
| 4403 | | |
| 4404 | | 2 models selected |
| 4405 | | |
| 4406 | | > view matrix models |
| 4407 | | > #9,0.18919,0.68161,-0.70683,127.56,-0.072128,0.72753,0.68227,-34.663,0.97929,-0.078095,0.1868,-5.196 |
| 4408 | | |
| 4409 | | > ui mousemode right "rotate selected models" |
| 4410 | | |
| 4411 | | > view matrix models |
| 4412 | | > #9,0.094504,0.99539,-0.016524,13.65,-0.46578,0.02954,-0.88441,305.71,-0.87984,0.091276,0.46642,193.56 |
| 4413 | | |
| 4414 | | > ui mousemode right "translate selected models" |
| 4415 | | |
| 4416 | | > view matrix models |
| 4417 | | > #9,0.094504,0.99539,-0.016524,15.801,-0.46578,0.02954,-0.88441,306.87,-0.87984,0.091276,0.46642,176.78 |
| 4418 | | |
| 4419 | | > view matrix models |
| 4420 | | > #9,0.094504,0.99539,-0.016524,12.042,-0.46578,0.02954,-0.88441,307.73,-0.87984,0.091276,0.46642,167.03 |
| 4421 | | |
| 4422 | | > ui mousemode right "rotate selected models" |
| 4423 | | |
| 4424 | | > view matrix models |
| 4425 | | > #9,0.21351,-0.97318,0.085687,220.62,-0.11603,0.061829,0.99132,14.069,-0.97003,-0.2216,-0.099713,290.21 |
| 4426 | | |
| 4427 | | > ui mousemode right "translate selected models" |
| 4428 | | |
| 4429 | | > view matrix models |
| 4430 | | > #9,0.21351,-0.97318,0.085687,223.99,-0.11603,0.061829,0.99132,9.6501,-0.97003,-0.2216,-0.099713,279.71 |
| 4431 | | |
| 4432 | | > fitmap #9 inMap #10 |
| 4433 | | |
| 4434 | | Fit map cryosparc_P4_J242_class_01_00122_volume_sharp.mrc in map |
| 4435 | | cryosparc_P4_J242_class_02_00122_volume_sharp.mrc using 29766 points |
| 4436 | | correlation = 0.8261, correlation about mean = 0.3518, overlap = 1268 |
| 4437 | | steps = 228, shift = 10.5, angle = 43.8 degrees |
| 4438 | | |
| 4439 | | Position of cryosparc_P4_J242_class_01_00122_volume_sharp.mrc (#9) relative to |
| 4440 | | cryosparc_P4_J242_class_02_00122_volume_sharp.mrc (#10) coordinates: |
| 4441 | | Matrix rotation and translation |
| 4442 | | -0.79231406 -0.50183310 0.34698987 259.09489231 |
| 4443 | | 0.60835065 -0.60661249 0.51179172 70.83831660 |
| 4444 | | -0.04634564 0.61659129 0.78591810 -47.09681091 |
| 4445 | | Axis 0.08862868 0.33264264 0.93887903 |
| 4446 | | Axis point 119.55771099 88.88471782 0.00000000 |
| 4447 | | Rotation angle (degrees) 143.75577737 |
| 4448 | | Shift along axis 2.30887434 |
| 4449 | | |
| 4450 | | |
| 4451 | | > select subtract #9 |
| 4452 | | |
| 4453 | | Nothing selected |
| 4454 | | |
| 4455 | | > hide #!9 models |
| 4456 | | |
| 4457 | | > hide #!10 models |
| 4458 | | |
| 4459 | | > show #!9 models |
| 4460 | | |
| 4461 | | > show #!10 models |
| 4462 | | |
| 4463 | | > hide #!10 models |
| 4464 | | |
| 4465 | | > show #!8 models |
| 4466 | | |
| 4467 | | > hide #!9 models |
| 4468 | | |
| 4469 | | > show #!9 models |
| 4470 | | |
| 4471 | | > select add #8 |
| 4472 | | |
| 4473 | | 2 models selected |
| 4474 | | |
| 4475 | | > ui mousemode right "rotate selected models" |
| 4476 | | |
| 4477 | | > view matrix models |
| 4478 | | > #8,0.57009,0.77789,0.26434,-76.801,0.087184,0.26265,-0.96094,232.01,-0.81694,0.57087,0.081917,150.81 |
| 4479 | | |
| 4480 | | > ui mousemode right "translate selected models" |
| 4481 | | |
| 4482 | | > view matrix models |
| 4483 | | > #8,0.57009,0.77789,0.26434,-65.422,0.087184,0.26265,-0.96094,221.32,-0.81694,0.57087,0.081917,140.75 |
| 4484 | | |
| 4485 | | > view matrix models |
| 4486 | | > #8,0.57009,0.77789,0.26434,-76.259,0.087184,0.26265,-0.96094,220.33,-0.81694,0.57087,0.081917,139.28 |
| 4487 | | |
| 4488 | | > ui mousemode right "rotate selected models" |
| 4489 | | |
| 4490 | | > view matrix models |
| 4491 | | > #8,0.35163,-0.43011,0.83148,20.753,0.096433,0.90012,0.42483,-70.009,-0.93116,-0.069204,0.35799,194.15 |
| 4492 | | |
| 4493 | | > ui mousemode right "translate selected models" |
| 4494 | | |
| 4495 | | > view matrix models |
| 4496 | | > #8,0.35163,-0.43011,0.83148,32.249,0.096433,0.90012,0.42483,-69.075,-0.93116,-0.069204,0.35799,199.19 |
| 4497 | | |
| 4498 | | > fitmap #8 inMap #9 |
| 4499 | | |
| 4500 | | Fit map cryosparc_P4_J242_class_00_00122_volume_sharp.mrc in map |
| 4501 | | cryosparc_P4_J242_class_01_00122_volume_sharp.mrc using 14754 points |
| 4502 | | correlation = 0.886, correlation about mean = 0.3322, overlap = 930.7 |
| 4503 | | steps = 164, shift = 11.7, angle = 31.5 degrees |
| 4504 | | |
| 4505 | | Position of cryosparc_P4_J242_class_00_00122_volume_sharp.mrc (#8) relative to |
| 4506 | | cryosparc_P4_J242_class_01_00122_volume_sharp.mrc (#9) coordinates: |
| 4507 | | Matrix rotation and translation |
| 4508 | | 0.99392076 -0.10814474 0.02064566 12.78899705 |
| 4509 | | -0.02517844 -0.40582068 -0.91360584 308.93876287 |
| 4510 | | 0.10718010 0.90753198 -0.40607651 52.31471926 |
| 4511 | | Axis 0.99784036 -0.04741407 0.04545901 |
| 4512 | | Axis point 0.00000000 137.80284162 126.13315110 |
| 4513 | | Rotation angle (degrees) 114.14129203 |
| 4514 | | Shift along axis 0.49150880 |
| 4515 | | |
| 4516 | | |
| 4517 | | > select subtract #8 |
| 4518 | | |
| 4519 | | Nothing selected |
| 4520 | | |
| 4521 | | > hide #!9 models |
| 4522 | | |
| 4523 | | > show #!9 models |
| 4524 | | |
| 4525 | | > hide #!8 models |
| 4526 | | |
| 4527 | | > show #!8 models |
| 4528 | | |
| 4529 | | > hide #!9 models |
| 4530 | | |
| 4531 | | > view P1 |
| 4532 | | |
| 4533 | | > show #!9 models |
| 4534 | | |
| 4535 | | > hide #!8 models |
| 4536 | | |
| 4537 | | > hide #!9 models |
| 4538 | | |
| 4539 | | > show #!10 models |
| 4540 | | |
| 4541 | | > hide #!10 models |
| 4542 | | |
| 4543 | | > show #!11 models |
| 4544 | | |
| 4545 | | > hide #!11 models |
| 4546 | | |
| 4547 | | > show #!12 models |
| 4548 | | |
| 4549 | | > hide #!12 models |
| 4550 | | |
| 4551 | | > show #!8 models |
| 4552 | | |
| 4553 | | > show #!12 models |
| 4554 | | |
| 4555 | | > view P1 |
| 4556 | | |
| 4557 | | > select add #8 |
| 4558 | | |
| 4559 | | 2 models selected |
| 4560 | | |
| 4561 | | > ui mousemode right "rotate selected models" |
| 4562 | | |
| 4563 | | > view matrix models |
| 4564 | | > #8,0.17056,0.37082,0.91291,-61.936,0.033776,0.92374,-0.38153,50.195,-0.98477,0.095906,0.14502,217.51 |
| 4565 | | |
| 4566 | | > select subtract #8 |
| 4567 | | |
| 4568 | | Nothing selected |
| 4569 | | |
| 4570 | | > hide #!12 models |
| 4571 | | |
| 4572 | | > show #!9 models |
| 4573 | | |
| 4574 | | > select add #9 |
| 4575 | | |
| 4576 | | 2 models selected |
| 4577 | | |
| 4578 | | > view matrix models |
| 4579 | | > #9,0.22431,-0.86548,-0.44791,277.63,0.10958,-0.43431,0.89407,50.356,-0.96834,-0.24963,-0.0025846,282.76 |
| 4580 | | |
| 4581 | | > fitmap #9 inMap #8 |
| 4582 | | |
| 4583 | | Fit map cryosparc_P4_J242_class_01_00122_volume_sharp.mrc in map |
| 4584 | | cryosparc_P4_J242_class_00_00122_volume_sharp.mrc using 29766 points |
| 4585 | | correlation = 0.8496, correlation about mean = 0.3079, overlap = 1328 |
| 4586 | | steps = 196, shift = 4.92, angle = 23.1 degrees |
| 4587 | | |
| 4588 | | Position of cryosparc_P4_J242_class_01_00122_volume_sharp.mrc (#9) relative to |
| 4589 | | cryosparc_P4_J242_class_00_00122_volume_sharp.mrc (#8) coordinates: |
| 4590 | | Matrix rotation and translation |
| 4591 | | 0.99577299 -0.02696715 0.08780046 -8.42294333 |
| 4592 | | -0.09023986 -0.46536786 0.88050527 89.20116331 |
| 4593 | | 0.01711480 -0.88470646 -0.46583426 307.74421532 |
| 4594 | | Axis -0.99855897 0.03998603 -0.03579261 |
| 4595 | | Axis point 0.00000000 137.24012810 127.13677639 |
| 4596 | | Rotation angle (degrees) 117.88604570 |
| 4597 | | Shift along axis 0.96263749 |
| 4598 | | |
| 4599 | | |
| 4600 | | > select subtract #9 |
| 4601 | | |
| 4602 | | Nothing selected |
| 4603 | | |
| 4604 | | > select add #9 |
| 4605 | | |
| 4606 | | 2 models selected |
| 4607 | | |
| 4608 | | > select subtract #9 |
| 4609 | | |
| 4610 | | Nothing selected |
| 4611 | | |
| 4612 | | > hide #!8 models |
| 4613 | | |
| 4614 | | > hide #!9 models |
| 4615 | | |
| 4616 | | > show #!9 models |
| 4617 | | |
| 4618 | | > show #!10 models |
| 4619 | | |
| 4620 | | > show #!8 models |
| 4621 | | |
| 4622 | | > hide #!9 models |
| 4623 | | |
| 4624 | | > hide #!8 models |
| 4625 | | |
| 4626 | | > show #!9 models |
| 4627 | | |
| 4628 | | > select add #10 |
| 4629 | | |
| 4630 | | 2 models selected |
| 4631 | | |
| 4632 | | > view matrix models |
| 4633 | | > #10,-0.15057,0.45945,-0.87535,204.11,-0.21614,0.84872,0.48265,-19.059,0.96468,0.26187,-0.028487,-39.785 |
| 4634 | | |
| 4635 | | > fitmap #10 inMap #8 |
| 4636 | | |
| 4637 | | Fit map cryosparc_P4_J242_class_02_00122_volume_sharp.mrc in map |
| 4638 | | cryosparc_P4_J242_class_00_00122_volume_sharp.mrc using 20970 points |
| 4639 | | correlation = 0.8656, correlation about mean = 0.3963, overlap = 1232 |
| 4640 | | steps = 148, shift = 6.34, angle = 30.8 degrees |
| 4641 | | |
| 4642 | | Position of cryosparc_P4_J242_class_02_00122_volume_sharp.mrc (#10) relative |
| 4643 | | to cryosparc_P4_J242_class_00_00122_volume_sharp.mrc (#8) coordinates: |
| 4644 | | Matrix rotation and translation |
| 4645 | | -0.99459983 0.06769619 -0.07866640 263.08124995 |
| 4646 | | 0.09074118 0.93510189 -0.34256458 42.75279763 |
| 4647 | | 0.05037079 -0.34785296 -0.93619501 298.73878944 |
| 4648 | | Axis -0.04031220 -0.98362390 0.17566718 |
| 4649 | | Axis point 125.67832095 0.00000000 157.57753081 |
| 4650 | | Rotation angle (degrees) 176.23911220 |
| 4651 | | Shift along axis -0.17945663 |
| 4652 | | |
| 4653 | | |
| 4654 | | > show #!8 models |
| 4655 | | |
| 4656 | | > hide #!9 models |
| 4657 | | |
| 4658 | | > select subtract #10 |
| 4659 | | |
| 4660 | | Nothing selected |
| 4661 | | |
| 4662 | | > hide #!10 models |
| 4663 | | |
| 4664 | | > show #!10 models |
| 4665 | | |
| 4666 | | > hide #!8 models |
| 4667 | | |
| 4668 | | > show #!8 models |
| 4669 | | |
| 4670 | | > hide #!8 models |
| 4671 | | |
| 4672 | | > show #!11 models |
| 4673 | | |
| 4674 | | > hide #!10 models |
| 4675 | | |
| 4676 | | > show #!10 models |
| 4677 | | |
| 4678 | | > select add #11 |
| 4679 | | |
| 4680 | | 2 models selected |
| 4681 | | |
| 4682 | | > view matrix models |
| 4683 | | > #11,0.83789,-0.53148,-0.12435,93.216,-0.53452,-0.84509,0.010318,306.1,-0.11057,0.05782,-0.99219,291.46 |
| 4684 | | |
| 4685 | | > view matrix models |
| 4686 | | > #11,0.87567,0.38049,0.29737,-84.014,0.47018,-0.53126,-0.70477,229.85,-0.11018,0.75696,-0.64411,156.03 |
| 4687 | | |
| 4688 | | > view matrix models |
| 4689 | | > #11,0.99273,0.070823,-0.097356,-6.1982,0.016369,-0.88055,-0.47367,302.86,-0.11927,0.46863,-0.87531,225.51 |
| 4690 | | |
| 4691 | | > fitmap #11 inMap #10 |
| 4692 | | |
| 4693 | | Fit map cryosparc_P4_J242_class_03_00122_volume_sharp.mrc in map |
| 4694 | | cryosparc_P4_J242_class_02_00122_volume_sharp.mrc using 45754 points |
| 4695 | | correlation = 0.7386, correlation about mean = -0.02581, overlap = 1355 |
| 4696 | | steps = 168, shift = 17.3, angle = 31.8 degrees |
| 4697 | | |
| 4698 | | Position of cryosparc_P4_J242_class_03_00122_volume_sharp.mrc (#11) relative |
| 4699 | | to cryosparc_P4_J242_class_02_00122_volume_sharp.mrc (#10) coordinates: |
| 4700 | | Matrix rotation and translation |
| 4701 | | -0.38917965 0.05095816 -0.91975131 323.90582539 |
| 4702 | | -0.15837916 -0.98730156 0.01231516 285.52055130 |
| 4703 | | -0.90744434 0.15046225 0.39230839 175.68159882 |
| 4704 | | Axis 0.55013716 -0.04900950 -0.83363492 |
| 4705 | | Axis point 230.74773970 125.72052254 0.00000000 |
| 4706 | | Rotation angle (degrees) 172.78707980 |
| 4707 | | Shift along axis 17.74509610 |
| 4708 | | |
| 4709 | | |
| 4710 | | > select subtract #11 |
| 4711 | | |
| 4712 | | Nothing selected |
| 4713 | | |
| 4714 | | > hide #!11 models |
| 4715 | | |
| 4716 | | > hide #!10 models |
| 4717 | | |
| 4718 | | > show #!11 models |
| 4719 | | |
| 4720 | | > hide #!11 models |
| 4721 | | |
| 4722 | | > show #!10 models |
| 4723 | | |
| 4724 | | > hide #!10 models |
| 4725 | | |
| 4726 | | > show #!9 models |
| 4727 | | |
| 4728 | | > hide #!9 models |
| 4729 | | |
| 4730 | | > view P1 |
| 4731 | | |
| 4732 | | > show #!12 models |
| 4733 | | |
| 4734 | | > ui mousemode right translate |
| 4735 | | |
| 4736 | | > save P4_J242_class_4_sharp.png supersample 3 transparentBackground true |
| 4737 | | |
| 4738 | | > hide #!12 models |
| 4739 | | |
| 4740 | | > show #!11 models |
| 4741 | | |
| 4742 | | > save P4_J242_class_3_sharp.png supersample 3 transparentBackground true |
| 4743 | | |
| 4744 | | > hide #!11 models |
| 4745 | | |
| 4746 | | > show #!10 models |
| 4747 | | |
| 4748 | | > save P4_J242_class_2_sharp.png supersample 3 transparentBackground true |
| 4749 | | |
| 4750 | | > hide #!10 models |
| 4751 | | |
| 4752 | | > show #!9 models |
| 4753 | | |
| 4754 | | > view P1 |
| 4755 | | |
| 4756 | | [Repeated 1 time(s)] |
| 4757 | | |
| 4758 | | > save P4_J242_class_1_sharp.png supersample 3 transparentBackground true |
| 4759 | | |
| 4760 | | > hide #!9 models |
| 4761 | | |
| 4762 | | > show #!8 models |
| 4763 | | |
| 4764 | | > save P4_J242_class_0_sharp.png supersample 3 transparentBackground true |
| 4765 | | |
| 4766 | | > save "/Users/peiyuyu/Documents/Goethe |
| 4767 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 4768 | | > F_B1/20260501_SA-F_B1_workflow.cxs" |
| 4769 | | |
| 4770 | | Unsupported scale factor (0.000000) detected on Display1 |
| 4771 | | |
| 4772 | | [Repeated 2 time(s)] |
| 4773 | | |
| 4774 | | > open "/Users/peiyuyu/Documents/Goethe |
| 4775 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 4776 | | > F_B1/cryosparc_P4_J117_class_00_00162_volume_sharp.mrc" |
| 4777 | | |
| 4778 | | Opened cryosparc_P4_J117_class_00_00162_volume_sharp.mrc as #13, grid size |
| 4779 | | 128,128,128, pixel 2.08, shown at level 0.163, step 1, values float32 |
| 4780 | | |
| 4781 | | > hide #!8 models |
| 4782 | | |
| 4783 | | > select add #13 |
| 4784 | | |
| 4785 | | 2 models selected |
| 4786 | | |
| 4787 | | > ui mousemode right "translate selected models" |
| 4788 | | |
| 4789 | | > view matrix models #13,1,0,0,6.2015,0,1,0,0.69431,0,0,1,-17.516 |
| 4790 | | |
| 4791 | | > ui mousemode right "rotate selected models" |
| 4792 | | |
| 4793 | | > view matrix models |
| 4794 | | > #13,-0.13264,-0.68512,-0.71625,337.99,0.0019176,-0.72281,0.69104,120.39,-0.99116,0.090284,0.097186,224.71 |
| 4795 | | |
| 4796 | | > view matrix models |
| 4797 | | > #13,0.0040424,0.12352,-0.99233,257.27,0.14964,-0.98124,-0.12153,245.92,-0.98873,-0.148,-0.02245,270.75 |
| 4798 | | |
| 4799 | | > select subtract #13 |
| 4800 | | |
| 4801 | | Nothing selected |
| 4802 | | |
| 4803 | | > show #!12 models |
| 4804 | | |
| 4805 | | > hide #!12 models |
| 4806 | | |
| 4807 | | > show #!11 models |
| 4808 | | |
| 4809 | | > hide #!11 models |
| 4810 | | |
| 4811 | | > show #!10 models |
| 4812 | | |
| 4813 | | > fitmap #13 inMap #10 |
| 4814 | | |
| 4815 | | Fit map cryosparc_P4_J117_class_00_00162_volume_sharp.mrc in map |
| 4816 | | cryosparc_P4_J242_class_02_00122_volume_sharp.mrc using 20961 points |
| 4817 | | correlation = 0.8109, correlation about mean = 0.2077, overlap = 1054 |
| 4818 | | steps = 92, shift = 12.7, angle = 18.1 degrees |
| 4819 | | |
| 4820 | | Position of cryosparc_P4_J117_class_00_00162_volume_sharp.mrc (#13) relative |
| 4821 | | to cryosparc_P4_J242_class_02_00122_volume_sharp.mrc (#10) coordinates: |
| 4822 | | Matrix rotation and translation |
| 4823 | | -0.98193657 -0.07856914 0.17212633 243.32837219 |
| 4824 | | 0.00195692 -0.91387700 -0.40598645 309.66348462 |
| 4825 | | 0.18920029 -0.39831610 0.89752300 41.87114917 |
| 4826 | | Axis 0.09277951 -0.20652448 0.97403265 |
| 4827 | | Axis point 116.38449758 161.85402651 0.00000000 |
| 4828 | | Rotation angle (degrees) 177.63092236 |
| 4829 | | Shift along axis -0.59333628 |
| 4830 | | |
| 4831 | | |
| 4832 | | > hide #!10 models |
| 4833 | | |
| 4834 | | > hide #!13 models |
| 4835 | | |
| 4836 | | > open "/Users/peiyuyu/Documents/Goethe |
| 4837 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 4838 | | > F_B1/cryosparc_P4_J117_class_01_00162_volume_sharp.mrc" |
| 4839 | | |
| 4840 | | Opened cryosparc_P4_J117_class_01_00162_volume_sharp.mrc as #14, grid size |
| 4841 | | 128,128,128, pixel 2.08, shown at level 0.187, step 1, values float32 |
| 4842 | | |
| 4843 | | > select add #14 |
| 4844 | | |
| 4845 | | 2 models selected |
| 4846 | | |
| 4847 | | > view matrix models |
| 4848 | | > #14,-0.39254,-0.77748,0.49135,234.79,-0.04345,-0.51796,-0.8543,320.26,0.91871,-0.3567,0.16954,27.288 |
| 4849 | | |
| 4850 | | > view matrix models |
| 4851 | | > #14,-0.061348,0.89979,0.432,-25.376,-0.288,-0.43036,0.85548,115.2,0.95566,-0.071931,0.28554,-30.976 |
| 4852 | | |
| 4853 | | > view matrix models |
| 4854 | | > #14,-0.046716,0.65995,-0.74985,161.94,-0.070464,0.74662,0.6615,-44.914,0.99642,0.08374,0.011624,-20.712 |
| 4855 | | |
| 4856 | | > select subtract #14 |
| 4857 | | |
| 4858 | | Nothing selected |
| 4859 | | |
| 4860 | | > show #!13 models |
| 4861 | | |
| 4862 | | > fitmap #14 inMap #13 |
| 4863 | | |
| 4864 | | Fit map cryosparc_P4_J117_class_01_00162_volume_sharp.mrc in map |
| 4865 | | cryosparc_P4_J117_class_00_00162_volume_sharp.mrc using 20968 points |
| 4866 | | correlation = 0.846, correlation about mean = 0.3465, overlap = 1110 |
| 4867 | | steps = 132, shift = 18.5, angle = 19.5 degrees |
| 4868 | | |
| 4869 | | Position of cryosparc_P4_J117_class_01_00162_volume_sharp.mrc (#14) relative |
| 4870 | | to cryosparc_P4_J117_class_00_00162_volume_sharp.mrc (#13) coordinates: |
| 4871 | | Matrix rotation and translation |
| 4872 | | -0.99955515 0.02982234 0.00035424 260.68209471 |
| 4873 | | -0.00628473 -0.19900464 -0.97997839 284.54003350 |
| 4874 | | -0.02915475 -0.97954468 0.19910354 244.04930876 |
| 4875 | | Axis 0.00930049 0.63278447 -0.77427212 |
| 4876 | | Axis point 133.98017190 240.03303746 0.00000000 |
| 4877 | | Rotation angle (degrees) 178.66392593 |
| 4878 | | Shift along axis -6.48359029 |
| 4879 | | |
| 4880 | | |
| 4881 | | > open "/Users/peiyuyu/Documents/Goethe |
| 4882 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 4883 | | > F_B1/cryosparc_P4_J117_class_02_00162_volume_sharp.mrc" |
| 4884 | | |
| 4885 | | Opened cryosparc_P4_J117_class_02_00162_volume_sharp.mrc as #15, grid size |
| 4886 | | 128,128,128, pixel 2.08, shown at level 0.251, step 1, values float32 |
| 4887 | | |
| 4888 | | > hide #!13 models |
| 4889 | | |
| 4890 | | > select add #14 |
| 4891 | | |
| 4892 | | 2 models selected |
| 4893 | | |
| 4894 | | > show #!12 models |
| 4895 | | |
| 4896 | | > select subtract #14 |
| 4897 | | |
| 4898 | | Nothing selected |
| 4899 | | |
| 4900 | | > hide #!14 models |
| 4901 | | |
| 4902 | | > select add #15 |
| 4903 | | |
| 4904 | | 2 models selected |
| 4905 | | |
| 4906 | | > view matrix models |
| 4907 | | > #15,-0.98221,0.18526,0.030552,219.51,0.18651,0.98144,0.044675,-26.394,-0.021708,0.049578,-0.99853,243.73 |
| 4908 | | |
| 4909 | | > ui mousemode right "translate selected models" |
| 4910 | | |
| 4911 | | > view matrix models |
| 4912 | | > #15,-0.98221,0.18526,0.030552,234.62,0.18651,0.98144,0.044675,-36.86,-0.021708,0.049578,-0.99853,271.78 |
| 4913 | | |
| 4914 | | > fitmap #15 inMap #12 |
| 4915 | | |
| 4916 | | Fit map cryosparc_P4_J117_class_02_00162_volume_sharp.mrc in map |
| 4917 | | cryosparc_P4_J242_class_04_00122_volume_sharp.mrc using 20967 points |
| 4918 | | correlation = 0.781, correlation about mean = 0.07908, overlap = 1573 |
| 4919 | | steps = 76, shift = 10.9, angle = 6.65 degrees |
| 4920 | | |
| 4921 | | Position of cryosparc_P4_J117_class_02_00162_volume_sharp.mrc (#15) relative |
| 4922 | | to cryosparc_P4_J242_class_04_00122_volume_sharp.mrc (#12) coordinates: |
| 4923 | | Matrix rotation and translation |
| 4924 | | 0.82536305 0.56130744 0.06090799 -57.36751075 |
| 4925 | | -0.56057744 0.82755504 -0.03009290 101.38226037 |
| 4926 | | -0.06729608 -0.00930608 0.99768964 1.14989876 |
| 4927 | | Axis 0.01840555 0.11351744 -0.99336550 |
| 4928 | | Axis point 134.55599767 144.37243816 0.00000000 |
| 4929 | | Rotation angle (degrees) 34.38068857 |
| 4930 | | Shift along axis 9.31050435 |
| 4931 | | |
| 4932 | | |
| 4933 | | > volume flip #15 |
| 4934 | | |
| 4935 | | Opened cryosparc_P4_J117_class_02_00162_volume_sharp.mrc z flip as #16, grid |
| 4936 | | size 128,128,128, pixel 2.08, shown at step 1, values float32 |
| 4937 | | |
| 4938 | | > close #15 |
| 4939 | | |
| 4940 | | > select add #16 |
| 4941 | | |
| 4942 | | 2 models selected |
| 4943 | | |
| 4944 | | > ui mousemode right "rotate selected models" |
| 4945 | | |
| 4946 | | > view matrix models |
| 4947 | | > #16,-0.81511,-0.57721,0.04929,312.41,0.52972,-0.77708,-0.33992,218.14,0.2345,-0.25096,0.93916,8.6526 |
| 4948 | | |
| 4949 | | > fitmap #15 inMap #12 |
| 4950 | | |
| 4951 | | No atoms or maps for #15 |
| 4952 | | |
| 4953 | | > fitmap #16 inMap #12 |
| 4954 | | |
| 4955 | | Fit map cryosparc_P4_J117_class_02_00162_volume_sharp.mrc z flip in map |
| 4956 | | cryosparc_P4_J242_class_04_00122_volume_sharp.mrc using 20967 points |
| 4957 | | correlation = 0.9777, correlation about mean = 0.7437, overlap = 2747 |
| 4958 | | steps = 108, shift = 14.1, angle = 17.5 degrees |
| 4959 | | |
| 4960 | | Position of cryosparc_P4_J117_class_02_00162_volume_sharp.mrc z flip (#16) |
| 4961 | | relative to cryosparc_P4_J242_class_04_00122_volume_sharp.mrc (#12) |
| 4962 | | coordinates: |
| 4963 | | Matrix rotation and translation |
| 4964 | | 0.94024990 -0.32652551 -0.09649458 65.60674301 |
| 4965 | | -0.33221344 -0.94188358 -0.04989541 307.74881291 |
| 4966 | | -0.07459454 0.07897095 -0.99408211 253.25354142 |
| 4967 | | Axis 0.98493295 -0.16738329 -0.04347315 |
| 4968 | | Axis point 0.00000000 155.23786381 133.19610246 |
| 4969 | | Rotation angle (degrees) 176.24909728 |
| 4970 | | Shift along axis 2.09650544 |
| 4971 | | |
| 4972 | | |
| 4973 | | > select subtract #16 |
| 4974 | | |
| 4975 | | Nothing selected |
| 4976 | | |
| 4977 | | > rename #16 CS_P4_J117_class_02_sharp_flip.mrc id #15 |
| 4978 | | |
| 4979 | | > save CS_P4_J117_class_02_sharp_flip.mrc #15 |
| 4980 | | |
| 4981 | | > open "/Users/peiyuyu/Documents/Goethe |
| 4982 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 4983 | | > F_B1/cryosparc_P4_J117_class_03_00162_volume_sharp.mrc" |
| 4984 | | |
| 4985 | | Opened cryosparc_P4_J117_class_03_00162_volume_sharp.mrc as #16, grid size |
| 4986 | | 128,128,128, pixel 2.08, shown at level 0.179, step 1, values float32 |
| 4987 | | |
| 4988 | | > hide #!12 models |
| 4989 | | |
| 4990 | | > hide #!15 models |
| 4991 | | |
| 4992 | | > select add #16 |
| 4993 | | |
| 4994 | | 2 models selected |
| 4995 | | |
| 4996 | | > show #!14 models |
| 4997 | | |
| 4998 | | > view matrix models |
| 4999 | | > #16,0.00021536,0.34795,0.93751,-37.63,0.50671,0.80821,-0.30007,0.053261,-0.86212,0.47511,-0.17613,183.13 |
| 5000 | | |
| 5001 | | > hide #!14 models |
| 5002 | | |
| 5003 | | > view matrix models |
| 5004 | | > #16,0.63015,0.10301,-0.76961,125.95,-0.74582,0.35606,-0.56301,261.02,0.21603,0.92877,0.3012,-81.743 |
| 5005 | | |
| 5006 | | > view matrix models |
| 5007 | | > #16,0.63277,-0.43368,-0.64151,190.37,0.75188,0.14602,0.64293,-47.242,-0.18515,-0.88916,0.41847,227.83 |
| 5008 | | |
| 5009 | | > show #!13 models |
| 5010 | | |
| 5011 | | > hide #!13 models |
| 5012 | | |
| 5013 | | > show #!12 models |
| 5014 | | |
| 5015 | | > hide #!12 models |
| 5016 | | |
| 5017 | | > show #!11 models |
| 5018 | | |
| 5019 | | > ui mousemode right "translate selected models" |
| 5020 | | |
| 5021 | | > view matrix models |
| 5022 | | > #16,0.63277,-0.43368,-0.64151,206.09,0.75188,0.14602,0.64293,-70.694,-0.18515,-0.88916,0.41847,249.05 |
| 5023 | | |
| 5024 | | > view matrix models |
| 5025 | | > #16,0.63277,-0.43368,-0.64151,204.5,0.75188,0.14602,0.64293,-75.196,-0.18515,-0.88916,0.41847,240.94 |
| 5026 | | |
| 5027 | | > ui mousemode right "rotate selected models" |
| 5028 | | |
| 5029 | | > view matrix models |
| 5030 | | > #16,-0.30976,-0.38252,-0.87047,346.06,0.94397,-0.2334,-0.23335,63.654,-0.1139,-0.89398,0.43339,230.67 |
| 5031 | | |
| 5032 | | > fitmap #16 inMap #11 |
| 5033 | | |
| 5034 | | Fit map cryosparc_P4_J117_class_03_00162_volume_sharp.mrc in map |
| 5035 | | cryosparc_P4_J242_class_03_00122_volume_sharp.mrc using 20958 points |
| 5036 | | correlation = 0.9023, correlation about mean = 0.3197, overlap = 1117 |
| 5037 | | steps = 184, shift = 6.54, angle = 53.8 degrees |
| 5038 | | |
| 5039 | | Position of cryosparc_P4_J117_class_03_00162_volume_sharp.mrc (#16) relative |
| 5040 | | to cryosparc_P4_J242_class_03_00122_volume_sharp.mrc (#11) coordinates: |
| 5041 | | Matrix rotation and translation |
| 5042 | | 0.41743274 -0.19488532 -0.88756388 222.32976103 |
| 5043 | | -0.90862481 -0.10271919 -0.40478358 322.57606660 |
| 5044 | | -0.01228347 0.97543246 -0.21995599 21.59894000 |
| 5045 | | Axis 0.77392148 -0.49079151 -0.40021149 |
| 5046 | | Axis point 0.00000000 222.40854643 197.20887777 |
| 5047 | | Rotation angle (degrees) 116.91198346 |
| 5048 | | Shift along axis 5.10403978 |
| 5049 | | |
| 5050 | | |
| 5051 | | > select subtract #16 |
| 5052 | | |
| 5053 | | Nothing selected |
| 5054 | | |
| 5055 | | > hide #!11 models |
| 5056 | | |
| 5057 | | > open "/Users/peiyuyu/Documents/Goethe |
| 5058 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 5059 | | > F_B1/cryosparc_P4_J117_class_04_00162_volume_sharp.mrc" |
| 5060 | | |
| 5061 | | Opened cryosparc_P4_J117_class_04_00162_volume_sharp.mrc as #17, grid size |
| 5062 | | 128,128,128, pixel 2.08, shown at level 0.206, step 1, values float32 |
| 5063 | | |
| 5064 | | > hide #!16 models |
| 5065 | | |
| 5066 | | > select add #17 |
| 5067 | | |
| 5068 | | 2 models selected |
| 5069 | | |
| 5070 | | > show #!13 models |
| 5071 | | |
| 5072 | | > hide #!13 models |
| 5073 | | |
| 5074 | | > show #!11 models |
| 5075 | | |
| 5076 | | > hide #!11 models |
| 5077 | | |
| 5078 | | > show #!10 models |
| 5079 | | |
| 5080 | | > hide #!17 models |
| 5081 | | |
| 5082 | | > show #!17 models |
| 5083 | | |
| 5084 | | > view matrix models |
| 5085 | | > #17,-0.0034658,-0.079884,-0.9968,294.42,-0.15421,0.98492,-0.078396,35.543,0.98803,0.15344,-0.015732,-30.795 |
| 5086 | | |
| 5087 | | > fitmap #17 inMap #10 |
| 5088 | | |
| 5089 | | Fit map cryosparc_P4_J117_class_04_00162_volume_sharp.mrc in map |
| 5090 | | cryosparc_P4_J242_class_02_00122_volume_sharp.mrc using 20958 points |
| 5091 | | correlation = 0.8416, correlation about mean = 0.3036, overlap = 1170 |
| 5092 | | steps = 148, shift = 13.9, angle = 32.2 degrees |
| 5093 | | |
| 5094 | | Position of cryosparc_P4_J117_class_04_00162_volume_sharp.mrc (#17) relative |
| 5095 | | to cryosparc_P4_J242_class_02_00122_volume_sharp.mrc (#10) coordinates: |
| 5096 | | Matrix rotation and translation |
| 5097 | | 0.73519379 0.67627298 0.04631360 -60.93633283 |
| 5098 | | -0.67522557 0.73664284 -0.03778583 134.15115598 |
| 5099 | | -0.05967011 -0.00349222 0.99821205 3.94076312 |
| 5100 | | Axis 0.02528875 0.07815439 -0.99662047 |
| 5101 | | Axis point 141.29888305 145.62167557 0.00000000 |
| 5102 | | Rotation angle (degrees) 42.69072745 |
| 5103 | | Shift along axis 5.01605257 |
| 5104 | | |
| 5105 | | |
| 5106 | | > select subtract #17 |
| 5107 | | |
| 5108 | | Nothing selected |
| 5109 | | |
| 5110 | | > hide #!10 models |
| 5111 | | |
| 5112 | | > show #!10 models |
| 5113 | | |
| 5114 | | > hide #!17 models |
| 5115 | | |
| 5116 | | > save "/Users/peiyuyu/Documents/Goethe |
| 5117 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 5118 | | > F_B1/20260501_SA-F_B1_workflow.cxs" |
| 5119 | | |
| 5120 | | > show #!13 models |
| 5121 | | |
| 5122 | | > hide #!10 models |
| 5123 | | |
| 5124 | | > color #13 #FCEDFF |
| 5125 | | |
| 5126 | | > hide #!13 models |
| 5127 | | |
| 5128 | | > show #!14 models |
| 5129 | | |
| 5130 | | > color #14 #FCEDFF |
| 5131 | | |
| 5132 | | > show #!15 models |
| 5133 | | |
| 5134 | | > hide #!14 models |
| 5135 | | |
| 5136 | | > hide #!15 models |
| 5137 | | |
| 5138 | | > show #!16 models |
| 5139 | | |
| 5140 | | > color #16 #FCEDFF |
| 5141 | | |
| 5142 | | > hide #!16 models |
| 5143 | | |
| 5144 | | > show #!17 models |
| 5145 | | |
| 5146 | | > color #17 #FCEDFF |
| 5147 | | |
| 5148 | | > show #!15 models |
| 5149 | | |
| 5150 | | > hide #!17 models |
| 5151 | | |
| 5152 | | > color #15 #99BFE5 |
| 5153 | | |
| 5154 | | > volume #15 level 0.3349 |
| 5155 | | |
| 5156 | | > ui mousemode right translate |
| 5157 | | |
| 5158 | | > view P1 |
| 5159 | | |
| 5160 | | [Repeated 1 time(s)] |
| 5161 | | |
| 5162 | | > save P4_J117_class_2_sharp.png supersample 3 transparentBackground true |
| 5163 | | |
| 5164 | | > hide #!15 models |
| 5165 | | |
| 5166 | | > show #!13 models |
| 5167 | | |
| 5168 | | > save P4_J117_class_0_sharp.png supersample 3 transparentBackground true |
| 5169 | | |
| 5170 | | > hide #!13 models |
| 5171 | | |
| 5172 | | > show #!14 models |
| 5173 | | |
| 5174 | | > save P4_J117_class_1_sharp.png supersample 3 transparentBackground true |
| 5175 | | |
| 5176 | | > hide #!14 models |
| 5177 | | |
| 5178 | | > show #!16 models |
| 5179 | | |
| 5180 | | > save P4_J117_class_3_sharp.png supersample 3 transparentBackground true |
| 5181 | | |
| 5182 | | > hide #!16 models |
| 5183 | | |
| 5184 | | > show #!17 models |
| 5185 | | |
| 5186 | | > save P4_J117_class_4_sharp.png supersample 3 transparentBackground true |
| 5187 | | |
| 5188 | | [Repeated 1 time(s)] |
| 5189 | | |
| 5190 | | > save "/Users/peiyuyu/Documents/Goethe |
| 5191 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 5192 | | > F_B1/20260501_SA-F_B1_workflow.cxs" |
| 5193 | | |
| 5194 | | > open "/Users/peiyuyu/Documents/Goethe |
| 5195 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 5196 | | > F_B1/cryosparc_P4_J120_002_volume_map_sharp.mrc" |
| 5197 | | |
| 5198 | | Opened cryosparc_P4_J120_002_volume_map_sharp.mrc as #18, grid size 80,80,80, |
| 5199 | | pixel 3.32, shown at level 1.08, step 1, values float32 |
| 5200 | | |
| 5201 | | > hide #!17 models |
| 5202 | | |
| 5203 | | > show #!15 models |
| 5204 | | |
| 5205 | | > select add #18 |
| 5206 | | |
| 5207 | | 2 models selected |
| 5208 | | |
| 5209 | | > ui mousemode right "rotate selected models" |
| 5210 | | |
| 5211 | | > view matrix models |
| 5212 | | > #18,0.9199,0.35329,0.17023,-57.439,0.37414,-0.92071,-0.11099,219.04,0.11752,0.16579,-0.97913,208.75 |
| 5213 | | |
| 5214 | | > ui mousemode right "translate selected models" |
| 5215 | | |
| 5216 | | > view matrix models |
| 5217 | | > #18,0.9199,0.35329,0.17023,-56.147,0.37414,-0.92071,-0.11099,218.08,0.11752,0.16579,-0.97913,234.23 |
| 5218 | | |
| 5219 | | > ui mousemode right "rotate selected models" |
| 5220 | | |
| 5221 | | > view matrix models |
| 5222 | | > #18,0.47537,0.87487,0.092902,-59.652,0.86989,-0.48319,0.099115,73.008,0.1316,0.033698,-0.99073,251.23 |
| 5223 | | |
| 5224 | | > volume flip #18 |
| 5225 | | |
| 5226 | | Opened cryosparc_P4_J120_002_volume_map_sharp.mrc z flip as #19, grid size |
| 5227 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 5228 | | |
| 5229 | | > select subtract #18 |
| 5230 | | |
| 5231 | | Nothing selected |
| 5232 | | |
| 5233 | | > close #18 |
| 5234 | | |
| 5235 | | > select add #19 |
| 5236 | | |
| 5237 | | 2 models selected |
| 5238 | | |
| 5239 | | > view matrix models |
| 5240 | | > #19,0.011836,0.9987,-0.049536,1.5471,-0.96239,0.024824,0.27053,210.58,0.27141,0.044471,0.96144,-38.44 |
| 5241 | | |
| 5242 | | > ui mousemode right "translate selected models" |
| 5243 | | |
| 5244 | | > view matrix models |
| 5245 | | > #19,0.011836,0.9987,-0.049536,5.1894,-0.96239,0.024824,0.27053,209.75,0.27141,0.044471,0.96144,-23.291 |
| 5246 | | |
| 5247 | | > fitmap #19 inMap #15 |
| 5248 | | |
| 5249 | | Fit map cryosparc_P4_J120_002_volume_map_sharp.mrc z flip in map |
| 5250 | | CS_P4_J117_class_02_sharp_flip.mrc using 5118 points |
| 5251 | | correlation = 0.9182, correlation about mean = 0.533, overlap = 3573 |
| 5252 | | steps = 76, shift = 9.96, angle = 10.7 degrees |
| 5253 | | |
| 5254 | | Position of cryosparc_P4_J120_002_volume_map_sharp.mrc z flip (#19) relative |
| 5255 | | to CS_P4_J117_class_02_sharp_flip.mrc (#15) coordinates: |
| 5256 | | Matrix rotation and translation |
| 5257 | | -0.48058823 -0.86458262 0.14673736 288.72558748 |
| 5258 | | 0.85464108 -0.49926017 -0.14257599 104.76974006 |
| 5259 | | 0.19652884 0.05688743 0.97884638 -28.97188121 |
| 5260 | | Axis 0.11519876 -0.02875674 0.99292613 |
| 5261 | | Axis point 115.59153027 135.80137987 0.00000000 |
| 5262 | | Rotation angle (degrees) 120.03315219 |
| 5263 | | Shift along axis 1.48105620 |
| 5264 | | |
| 5265 | | |
| 5266 | | > ui mousemode right "rotate selected models" |
| 5267 | | |
| 5268 | | > view matrix models |
| 5269 | | > #19,0.89305,-0.32489,-0.3113,106.99,0.34183,0.93976,-0.00013805,-25.175,0.29259,-0.10629,0.95031,-3.3753 |
| 5270 | | |
| 5271 | | > ui mousemode right "translate selected models" |
| 5272 | | |
| 5273 | | > view matrix models |
| 5274 | | > #19,0.89305,-0.32489,-0.3113,96.178,0.34183,0.93976,-0.00013805,-42.263,0.29259,-0.10629,0.95031,-5.1551 |
| 5275 | | |
| 5276 | | > fitmap #19 inMap #15 |
| 5277 | | |
| 5278 | | Fit map cryosparc_P4_J120_002_volume_map_sharp.mrc z flip in map |
| 5279 | | CS_P4_J117_class_02_sharp_flip.mrc using 5118 points |
| 5280 | | correlation = 0.9187, correlation about mean = 0.5356, overlap = 3578 |
| 5281 | | steps = 76, shift = 8.31, angle = 8.52 degrees |
| 5282 | | |
| 5283 | | Position of cryosparc_P4_J120_002_volume_map_sharp.mrc z flip (#19) relative |
| 5284 | | to CS_P4_J117_class_02_sharp_flip.mrc (#15) coordinates: |
| 5285 | | Matrix rotation and translation |
| 5286 | | -0.47913034 0.85546358 0.19651003 54.96243137 |
| 5287 | | -0.86530880 -0.49790427 0.05772364 303.37608678 |
| 5288 | | 0.14722365 -0.14238471 0.97880120 3.73022055 |
| 5289 | | Axis -0.11546470 0.02843878 -0.99290440 |
| 5290 | | Axis point 115.58296139 135.79077530 0.00000000 |
| 5291 | | Rotation angle (degrees) 119.94157891 |
| 5292 | | Shift along axis -1.42232844 |
| 5293 | | |
| 5294 | | |
| 5295 | | > hide #!19 models |
| 5296 | | |
| 5297 | | > hide #!15 models |
| 5298 | | |
| 5299 | | > show #!15 models |
| 5300 | | |
| 5301 | | > hide #!15 models |
| 5302 | | |
| 5303 | | > show #!19 models |
| 5304 | | |
| 5305 | | > select subtract #19 |
| 5306 | | |
| 5307 | | Nothing selected |
| 5308 | | |
| 5309 | | > show #!15 models |
| 5310 | | |
| 5311 | | > hide #!15 models |
| 5312 | | |
| 5313 | | > rename #19 CS_P4_J120_sharp_flip id #18 |
| 5314 | | |
| 5315 | | > save CS_P4_J120_sharp_flip.mrc #18 |
| 5316 | | |
| 5317 | | > volume #18 level 1.185 |
| 5318 | | |
| 5319 | | > volume #18 level 1.639 |
| 5320 | | |
| 5321 | | > volume #18 step 2 |
| 5322 | | |
| 5323 | | > volume #18 step 1 |
| 5324 | | |
| 5325 | | > show #!15 models |
| 5326 | | |
| 5327 | | > volume #18 level 1.559 |
| 5328 | | |
| 5329 | | > hide #!15 models |
| 5330 | | |
| 5331 | | > volume #18 level 1.292 |
| 5332 | | |
| 5333 | | > view P1 |
| 5334 | | |
| 5335 | | > color #18 #99BFE5 |
| 5336 | | |
| 5337 | | > show #!17 models |
| 5338 | | |
| 5339 | | > hide #!17 models |
| 5340 | | |
| 5341 | | > hide #!18 models |
| 5342 | | |
| 5343 | | > show #!18 models |
| 5344 | | |
| 5345 | | > save P4_J120_NU_sharp.png supersample 3 transparentBackground true |
| 5346 | | |
| 5347 | | > save "/Users/peiyuyu/Documents/Goethe |
| 5348 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 5349 | | > F_B1/20260501_SA-F_B1_workflow.cxs" |
| 5350 | | |
| 5351 | | > open "/Users/peiyuyu/Documents/Goethe |
| 5352 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 5353 | | > F_B1/cryosparc_P4_J169_class_00_00182_volume_sharp.mrc" |
| 5354 | | |
| 5355 | | Opened cryosparc_P4_J169_class_00_00182_volume_sharp.mrc as #19, grid size |
| 5356 | | 128,128,128, pixel 2.08, shown at level 0.175, step 1, values float32 |
| 5357 | | |
| 5358 | | > select add #19 |
| 5359 | | |
| 5360 | | 2 models selected |
| 5361 | | |
| 5362 | | > hide #!18 models |
| 5363 | | |
| 5364 | | > show #!18 models |
| 5365 | | |
| 5366 | | > ui mousemode right "rotate selected models" |
| 5367 | | |
| 5368 | | > ui mousemode right "translate selected models" |
| 5369 | | |
| 5370 | | > view matrix models #19,1,0,0,-7.1681,0,1,0,-1.6755,0,0,1,29.407 |
| 5371 | | |
| 5372 | | > fitmap #19 inMap #18 |
| 5373 | | |
| 5374 | | Fit map cryosparc_P4_J169_class_00_00182_volume_sharp.mrc in map |
| 5375 | | CS_P4_J120_sharp_flip.mrc using 20970 points |
| 5376 | | correlation = 0.3797, correlation about mean = 0.05313, overlap = 2374 |
| 5377 | | steps = 192, shift = 6.4, angle = 17.5 degrees |
| 5378 | | |
| 5379 | | Position of cryosparc_P4_J169_class_00_00182_volume_sharp.mrc (#19) relative |
| 5380 | | to CS_P4_J120_sharp_flip.mrc (#18) coordinates: |
| 5381 | | Matrix rotation and translation |
| 5382 | | 0.64594502 0.66965380 0.36649532 -93.21942227 |
| 5383 | | -0.65111130 0.73392063 -0.19342850 149.32350272 |
| 5384 | | -0.39850860 -0.11368507 0.91009153 102.27587247 |
| 5385 | | Axis 0.05217441 0.50052557 -0.86414813 |
| 5386 | | Axis point 153.99219258 219.18137479 0.00000000 |
| 5387 | | Rotation angle (degrees) 49.83592965 |
| 5388 | | Shift along axis -18.50494005 |
| 5389 | | |
| 5390 | | |
| 5391 | | > select subtract #19 |
| 5392 | | |
| 5393 | | Nothing selected |
| 5394 | | |
| 5395 | | > hide #!18 models |
| 5396 | | |
| 5397 | | > open "/Users/peiyuyu/Documents/Goethe |
| 5398 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 5399 | | > F_B1/cryosparc_P4_J169_class_01_00182_volume_sharp.mrc" |
| 5400 | | |
| 5401 | | Opened cryosparc_P4_J169_class_01_00182_volume_sharp.mrc as #20, grid size |
| 5402 | | 128,128,128, pixel 2.08, shown at level 0.189, step 1, values float32 |
| 5403 | | |
| 5404 | | > hide #!19 models |
| 5405 | | |
| 5406 | | > show #!17 models |
| 5407 | | |
| 5408 | | > select add #20 |
| 5409 | | |
| 5410 | | 2 models selected |
| 5411 | | |
| 5412 | | > ui mousemode right "rotate selected models" |
| 5413 | | |
| 5414 | | > view matrix models |
| 5415 | | > #20,0.023382,0.1088,0.99379,-27.517,0.050205,0.99268,-0.10986,9.6432,-0.99847,0.052462,0.017749,251.64 |
| 5416 | | |
| 5417 | | > hide #!17 models |
| 5418 | | |
| 5419 | | > show #!16 models |
| 5420 | | |
| 5421 | | > hide #!16 models |
| 5422 | | |
| 5423 | | > show #!15 models |
| 5424 | | |
| 5425 | | > hide #!15 models |
| 5426 | | |
| 5427 | | > show #!14 models |
| 5428 | | |
| 5429 | | > ui mousemode right "translate selected models" |
| 5430 | | |
| 5431 | | > view matrix models |
| 5432 | | > #20,0.023382,0.1088,0.99379,-22.964,0.050205,0.99268,-0.10986,7.1023,-0.99847,0.052462,0.017749,242.74 |
| 5433 | | |
| 5434 | | > ui mousemode right "rotate selected models" |
| 5435 | | |
| 5436 | | > view matrix models |
| 5437 | | > #20,-0.029978,-0.71994,-0.69339,335.92,-0.21064,-0.67357,0.70847,177,-0.9771,0.16729,-0.13145,243.19 |
| 5438 | | |
| 5439 | | > ui mousemode right "translate selected models" |
| 5440 | | |
| 5441 | | > view matrix models |
| 5442 | | > #20,-0.029978,-0.71994,-0.69339,328.25,-0.21064,-0.67357,0.70847,170.9,-0.9771,0.16729,-0.13145,240.41 |
| 5443 | | |
| 5444 | | > fitmap #20 inMap #14 |
| 5445 | | |
| 5446 | | Fit map cryosparc_P4_J169_class_01_00182_volume_sharp.mrc in map |
| 5447 | | cryosparc_P4_J117_class_01_00162_volume_sharp.mrc using 20954 points |
| 5448 | | correlation = 0.8829, correlation about mean = 0.4124, overlap = 1200 |
| 5449 | | steps = 104, shift = 14, angle = 14 degrees |
| 5450 | | |
| 5451 | | Position of cryosparc_P4_J169_class_01_00182_volume_sharp.mrc (#20) relative |
| 5452 | | to cryosparc_P4_J117_class_01_00162_volume_sharp.mrc (#14) coordinates: |
| 5453 | | Matrix rotation and translation |
| 5454 | | -0.94773816 0.28655181 -0.14028702 243.79600144 |
| 5455 | | -0.24891252 -0.93914826 -0.23673424 326.23627964 |
| 5456 | | -0.19958693 -0.18944287 0.96139298 60.20896934 |
| 5457 | | Axis 0.08744550 0.10965027 -0.99011621 |
| 5458 | | Axis point 147.27291182 149.47535150 0.00000000 |
| 5459 | | Rotation angle (degrees) 164.31164420 |
| 5460 | | Shift along axis -2.52311906 |
| 5461 | | |
| 5462 | | |
| 5463 | | > select subtract #20 |
| 5464 | | |
| 5465 | | Nothing selected |
| 5466 | | |
| 5467 | | > hide #!14 models |
| 5468 | | |
| 5469 | | > open "/Users/peiyuyu/Documents/Goethe |
| 5470 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 5471 | | > F_B1/cryosparc_P4_J169_class_02_00182_volume_sharp.mrc" |
| 5472 | | |
| 5473 | | Opened cryosparc_P4_J169_class_02_00182_volume_sharp.mrc as #21, grid size |
| 5474 | | 128,128,128, pixel 2.08, shown at level 0.216, step 1, values float32 |
| 5475 | | |
| 5476 | | > hide #!20 models |
| 5477 | | |
| 5478 | | > select add #21 |
| 5479 | | |
| 5480 | | 2 models selected |
| 5481 | | |
| 5482 | | > view matrix models #21,1,0,0,-13.073,0,1,0,8.3491,0,0,1,8.6967 |
| 5483 | | |
| 5484 | | > ui mousemode right "rotate selected models" |
| 5485 | | |
| 5486 | | > view matrix models |
| 5487 | | > #21,-0.06365,0.67952,0.73089,-53.58,-0.2928,0.68743,-0.66461,177.89,-0.95405,-0.25631,0.15521,285.48 |
| 5488 | | |
| 5489 | | > view matrix models |
| 5490 | | > #21,0.10048,-0.44353,0.89061,41.096,0.13793,-0.88029,-0.45395,284.46,0.98533,0.16846,-0.027275,-4.3073 |
| 5491 | | |
| 5492 | | > fitmap #21 inMap #20 |
| 5493 | | |
| 5494 | | Fit map cryosparc_P4_J169_class_02_00182_volume_sharp.mrc in map |
| 5495 | | cryosparc_P4_J169_class_01_00182_volume_sharp.mrc using 20962 points |
| 5496 | | correlation = 0.7829, correlation about mean = 0.1539, overlap = 900.6 |
| 5497 | | steps = 188, shift = 21.5, angle = 32 degrees |
| 5498 | | |
| 5499 | | Position of cryosparc_P4_J169_class_02_00182_volume_sharp.mrc (#21) relative |
| 5500 | | to cryosparc_P4_J169_class_01_00182_volume_sharp.mrc (#20) coordinates: |
| 5501 | | Matrix rotation and translation |
| 5502 | | -0.75680258 0.56772680 0.32393846 109.96194933 |
| 5503 | | 0.37589927 0.78345603 -0.49487007 56.85693302 |
| 5504 | | -0.53474254 -0.25275071 -0.80632963 338.09031391 |
| 5505 | | Axis 0.26532092 0.94096581 -0.21020979 |
| 5506 | | Axis point 90.65236627 0.00000000 161.68382083 |
| 5507 | | Rotation angle (degrees) 152.85290905 |
| 5508 | | Shift along axis 11.60574300 |
| 5509 | | |
| 5510 | | |
| 5511 | | > show #!20 models |
| 5512 | | |
| 5513 | | > select subtract #21 |
| 5514 | | |
| 5515 | | Nothing selected |
| 5516 | | |
| 5517 | | > select add #21 |
| 5518 | | |
| 5519 | | 2 models selected |
| 5520 | | |
| 5521 | | > ui mousemode right "translate selected models" |
| 5522 | | |
| 5523 | | > view matrix models |
| 5524 | | > #21,0.31625,-0.43913,0.84092,24.723,-0.35094,-0.87769,-0.32634,336.73,0.88138,-0.19191,-0.43168,94.219 |
| 5525 | | |
| 5526 | | > ui mousemode right "rotate selected models" |
| 5527 | | |
| 5528 | | > view matrix models |
| 5529 | | > #21,0.23561,0.79493,-0.55908,75.399,0.21977,0.51679,0.82742,-71.321,0.94667,-0.31782,-0.052938,48.925 |
| 5530 | | |
| 5531 | | > ui mousemode right "translate selected models" |
| 5532 | | |
| 5533 | | > view matrix models |
| 5534 | | > #21,0.23561,0.79493,-0.55908,69.861,0.21977,0.51679,0.82742,-70.077,0.94667,-0.31782,-0.052938,40.098 |
| 5535 | | |
| 5536 | | > fitmap #21 inMap #20 |
| 5537 | | |
| 5538 | | Fit map cryosparc_P4_J169_class_02_00182_volume_sharp.mrc in map |
| 5539 | | cryosparc_P4_J169_class_01_00182_volume_sharp.mrc using 20962 points |
| 5540 | | correlation = 0.8883, correlation about mean = 0.4082, overlap = 1398 |
| 5541 | | steps = 80, shift = 8.95, angle = 4.79 degrees |
| 5542 | | |
| 5543 | | Position of cryosparc_P4_J169_class_02_00182_volume_sharp.mrc (#21) relative |
| 5544 | | to cryosparc_P4_J169_class_01_00182_volume_sharp.mrc (#20) coordinates: |
| 5545 | | Matrix rotation and translation |
| 5546 | | -0.99875153 -0.02706229 -0.04198798 281.31180443 |
| 5547 | | 0.03395891 -0.98426911 -0.17338138 286.61926959 |
| 5548 | | -0.03663538 -0.17459079 0.98395929 28.58358251 |
| 5549 | | Axis -0.01973976 -0.08736459 0.99598081 |
| 5550 | | Axis point 138.73615561 146.72655614 0.00000000 |
| 5551 | | Rotation angle (degrees) 178.24454217 |
| 5552 | | Shift along axis -2.12470384 |
| 5553 | | |
| 5554 | | |
| 5555 | | > select subtract #21 |
| 5556 | | |
| 5557 | | Nothing selected |
| 5558 | | |
| 5559 | | > hide #!20 models |
| 5560 | | |
| 5561 | | > hide #!21 models |
| 5562 | | |
| 5563 | | > show #!21 models |
| 5564 | | |
| 5565 | | > show #!20 models |
| 5566 | | |
| 5567 | | > hide #!21 models |
| 5568 | | |
| 5569 | | > show #!21 models |
| 5570 | | |
| 5571 | | > open "/Users/peiyuyu/Documents/Goethe |
| 5572 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 5573 | | > F_B1/cryosparc_P4_J169_class_03_00182_volume_sharp.mrc" |
| 5574 | | |
| 5575 | | Opened cryosparc_P4_J169_class_03_00182_volume_sharp.mrc as #22, grid size |
| 5576 | | 128,128,128, pixel 2.08, shown at level 0.248, step 1, values float32 |
| 5577 | | |
| 5578 | | > hide #!20 models |
| 5579 | | |
| 5580 | | > hide #!21 models |
| 5581 | | |
| 5582 | | > select add #22 |
| 5583 | | |
| 5584 | | 2 models selected |
| 5585 | | |
| 5586 | | > show #!18 models |
| 5587 | | |
| 5588 | | > ui mousemode right "rotate selected models" |
| 5589 | | |
| 5590 | | > view matrix models |
| 5591 | | > #22,0.80286,-0.54835,0.23395,74.113,-0.54307,-0.83458,-0.092472,336.96,0.24595,-0.052807,-0.96784,209.56 |
| 5592 | | |
| 5593 | | > view matrix models |
| 5594 | | > #22,0.23802,0.97115,0.01476,-30.605,0.97056,-0.2384,0.03436,32.801,0.036887,0.0061467,-0.9993,233.87 |
| 5595 | | |
| 5596 | | > ui mousemode right "translate selected models" |
| 5597 | | |
| 5598 | | > view matrix models |
| 5599 | | > #22,0.23802,0.97115,0.01476,-35.135,0.97056,-0.2384,0.03436,31.843,0.036887,0.0061467,-0.9993,265.72 |
| 5600 | | |
| 5601 | | > view matrix models |
| 5602 | | > #22,0.23802,0.97115,0.01476,-30.091,0.97056,-0.2384,0.03436,32.303,0.036887,0.0061467,-0.9993,265.71 |
| 5603 | | |
| 5604 | | > select subtract #22 |
| 5605 | | |
| 5606 | | Nothing selected |
| 5607 | | |
| 5608 | | > hide #!18 models |
| 5609 | | |
| 5610 | | > volume flip #22 |
| 5611 | | |
| 5612 | | Opened cryosparc_P4_J169_class_03_00182_volume_sharp.mrc z flip as #23, grid |
| 5613 | | size 128,128,128, pixel 2.08, shown at step 1, values float32 |
| 5614 | | |
| 5615 | | > close #22 |
| 5616 | | |
| 5617 | | > show #!21 models |
| 5618 | | |
| 5619 | | > hide #!21 models |
| 5620 | | |
| 5621 | | > show #!18 models |
| 5622 | | |
| 5623 | | > select add #23 |
| 5624 | | |
| 5625 | | 2 models selected |
| 5626 | | |
| 5627 | | > view matrix models |
| 5628 | | > #23,0.23802,0.97115,0.01476,-34.192,0.97056,-0.2384,0.03436,27.412,0.036887,0.0061467,-0.9993,278.73 |
| 5629 | | |
| 5630 | | > ui mousemode right "rotate selected models" |
| 5631 | | |
| 5632 | | > view matrix models |
| 5633 | | > #23,-0.23315,-0.92003,-0.31494,337.04,0.93397,-0.30205,0.19095,18.61,-0.27081,-0.24962,0.92971,78.293 |
| 5634 | | |
| 5635 | | > ui mousemode right "translate selected models" |
| 5636 | | |
| 5637 | | > view matrix models |
| 5638 | | > #23,-0.23315,-0.92003,-0.31494,335.86,0.93397,-0.30205,0.19095,18.866,-0.27081,-0.24962,0.92971,88.616 |
| 5639 | | |
| 5640 | | > fitmap #23 inMap #18 |
| 5641 | | |
| 5642 | | Fit map cryosparc_P4_J169_class_03_00182_volume_sharp.mrc z flip in map |
| 5643 | | CS_P4_J120_sharp_flip.mrc using 20952 points |
| 5644 | | correlation = 0.9077, correlation about mean = 0.6695, overlap = 1.202e+04 |
| 5645 | | steps = 60, shift = 4.34, angle = 10.2 degrees |
| 5646 | | |
| 5647 | | Position of cryosparc_P4_J169_class_03_00182_volume_sharp.mrc z flip (#23) |
| 5648 | | relative to CS_P4_J120_sharp_flip.mrc (#18) coordinates: |
| 5649 | | Matrix rotation and translation |
| 5650 | | 0.17479617 -0.97573598 0.13185442 219.49728105 |
| 5651 | | 0.98179238 0.18284123 0.05150543 -32.00811477 |
| 5652 | | -0.07436413 0.12045072 0.98993010 -9.54926019 |
| 5653 | | Axis 0.03500529 0.10470244 0.99388733 |
| 5654 | | Axis point 128.78581299 116.22335978 0.00000000 |
| 5655 | | Rotation angle (degrees) 79.99211268 |
| 5656 | | Shift along axis -5.15865059 |
| 5657 | | |
| 5658 | | |
| 5659 | | > select subtract #23 |
| 5660 | | |
| 5661 | | Nothing selected |
| 5662 | | |
| 5663 | | > hide #!18 models |
| 5664 | | |
| 5665 | | > rename #23 CS_P4_J169_class_03_sharp_flip id #22 |
| 5666 | | |
| 5667 | | > save CS_P4_J169_class_03_sharp_flip.mrc #22 |
| 5668 | | |
| 5669 | | > show #!21 models |
| 5670 | | |
| 5671 | | > hide #!21 models |
| 5672 | | |
| 5673 | | > show #!18 models |
| 5674 | | |
| 5675 | | > hide #!18 models |
| 5676 | | |
| 5677 | | > open "/Users/peiyuyu/Documents/Goethe |
| 5678 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 5679 | | > F_B1/cryosparc_P4_J169_class_04_00182_volume_sharp.mrc" |
| 5680 | | |
| 5681 | | Opened cryosparc_P4_J169_class_04_00182_volume_sharp.mrc as #23, grid size |
| 5682 | | 128,128,128, pixel 2.08, shown at level 0.171, step 1, values float32 |
| 5683 | | |
| 5684 | | > hide #!22 models |
| 5685 | | |
| 5686 | | > show #!21 models |
| 5687 | | |
| 5688 | | > select add #23 |
| 5689 | | |
| 5690 | | 2 models selected |
| 5691 | | |
| 5692 | | > view matrix models #23,1,0,0,62.54,0,1,0,-0.93224,0,0,1,-9.0713 |
| 5693 | | |
| 5694 | | > ui mousemode right "rotate selected models" |
| 5695 | | |
| 5696 | | > view matrix models |
| 5697 | | > #23,0.11131,-0.99321,-0.033862,304.62,-0.95598,-0.11633,0.2694,223.09,-0.27151,0.0023841,-0.96243,297.1 |
| 5698 | | |
| 5699 | | > view matrix models |
| 5700 | | > #23,0.14785,0.8725,0.4657,-3.2346,-0.24604,0.48852,-0.83714,210.48,-0.95792,0.0091875,0.2869,210.23 |
| 5701 | | |
| 5702 | | > ui mousemode right "translate selected models" |
| 5703 | | |
| 5704 | | > view matrix models |
| 5705 | | > #23,0.14785,0.8725,0.4657,-72.613,-0.24604,0.48852,-0.83714,211.81,-0.95792,0.0091875,0.2869,205.44 |
| 5706 | | |
| 5707 | | > ui mousemode right "rotate selected models" |
| 5708 | | |
| 5709 | | > view matrix models |
| 5710 | | > #23,-0.12372,0.72067,0.68215,-49.094,0.065146,0.69184,-0.7191,130.45,-0.99018,-0.044529,-0.13254,274.4 |
| 5711 | | |
| 5712 | | > ui mousemode right "translate selected models" |
| 5713 | | |
| 5714 | | > view matrix models |
| 5715 | | > #23,-0.12372,0.72067,0.68215,-52.059,0.065146,0.69184,-0.7191,130.58,-0.99018,-0.044529,-0.13254,270.2 |
| 5716 | | |
| 5717 | | > fitmap #23 inMap #21 |
| 5718 | | |
| 5719 | | Fit map cryosparc_P4_J169_class_04_00182_volume_sharp.mrc in map |
| 5720 | | cryosparc_P4_J169_class_02_00182_volume_sharp.mrc using 20956 points |
| 5721 | | correlation = 0.8996, correlation about mean = 0.4033, overlap = 1373 |
| 5722 | | steps = 168, shift = 14.7, angle = 39.5 degrees |
| 5723 | | |
| 5724 | | Position of cryosparc_P4_J169_class_04_00182_volume_sharp.mrc (#23) relative |
| 5725 | | to cryosparc_P4_J169_class_02_00182_volume_sharp.mrc (#21) coordinates: |
| 5726 | | Matrix rotation and translation |
| 5727 | | -0.99961182 0.01552465 -0.02313457 267.26750037 |
| 5728 | | 0.02397017 0.90245504 -0.43011668 70.06567251 |
| 5729 | | 0.01420050 -0.43050425 -0.90247684 305.57661651 |
| 5730 | | Axis -0.01012464 -0.97530665 0.22062281 |
| 5731 | | Axis point 131.76679627 0.00000000 162.02424501 |
| 5732 | | Rotation angle (degrees) 178.90328205 |
| 5733 | | Shift along axis -3.62433410 |
| 5734 | | |
| 5735 | | |
| 5736 | | > select subtract #23 |
| 5737 | | |
| 5738 | | Nothing selected |
| 5739 | | |
| 5740 | | > hide #!23 models |
| 5741 | | |
| 5742 | | > hide #!21 models |
| 5743 | | |
| 5744 | | > show #!23 models |
| 5745 | | |
| 5746 | | > volume #23 level 0.1996 |
| 5747 | | |
| 5748 | | > hide #!23 models |
| 5749 | | |
| 5750 | | > show #!22 models |
| 5751 | | |
| 5752 | | > volume #22 level 0.2715 |
| 5753 | | |
| 5754 | | > hide #!22 models |
| 5755 | | |
| 5756 | | > show #!21 models |
| 5757 | | |
| 5758 | | > volume #21 level 0.2481 |
| 5759 | | |
| 5760 | | > hide #!21 models |
| 5761 | | |
| 5762 | | > show #!20 models |
| 5763 | | |
| 5764 | | > volume #20 level 0.198 |
| 5765 | | |
| 5766 | | > hide #!20 models |
| 5767 | | |
| 5768 | | > show #!19 models |
| 5769 | | |
| 5770 | | > volume #19 level 0.1357 |
| 5771 | | |
| 5772 | | > volume #19 level 0.1565 |
| 5773 | | |
| 5774 | | > color #9 #FCEDFF |
| 5775 | | |
| 5776 | | > show #!9 models |
| 5777 | | |
| 5778 | | > hide #!9 models |
| 5779 | | |
| 5780 | | > color #19 #FCEDFF |
| 5781 | | |
| 5782 | | > save "/Users/peiyuyu/Documents/Goethe |
| 5783 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 5784 | | > F_B1/20260501_SA-F_B1_workflow.cxs" |
| 5785 | | |
| 5786 | | > hide #!19 models |
| 5787 | | |
| 5788 | | > show #!20 models |
| 5789 | | |
| 5790 | | > color #20 #FCEDFF |
| 5791 | | |
| 5792 | | > hide #!20 models |
| 5793 | | |
| 5794 | | > show #!21 models |
| 5795 | | |
| 5796 | | > show #!20 models |
| 5797 | | |
| 5798 | | > hide #!20 models |
| 5799 | | |
| 5800 | | > color #21 #FCEDFF |
| 5801 | | |
| 5802 | | > hide #!21 models |
| 5803 | | |
| 5804 | | > show #!22 models |
| 5805 | | |
| 5806 | | > show #!23 models |
| 5807 | | |
| 5808 | | > hide #!22 models |
| 5809 | | |
| 5810 | | > color #23 #FCEDFF |
| 5811 | | |
| 5812 | | > color #22 #99BFE5 |
| 5813 | | |
| 5814 | | > show #!22 models |
| 5815 | | |
| 5816 | | > hide #!23 models |
| 5817 | | |
| 5818 | | > hide #!22 models |
| 5819 | | |
| 5820 | | > show #!22 models |
| 5821 | | |
| 5822 | | > save "/Users/peiyuyu/Documents/Goethe |
| 5823 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 5824 | | > F_B1/20260501_SA-F_B1_workflow.cxs" |
| 5825 | | |
| 5826 | | > view P1 |
| 5827 | | |
| 5828 | | > save P4_J169_class_3_sharp.png supersample 3 transparentBackground true |
| 5829 | | |
| 5830 | | > hide #!22 models |
| 5831 | | |
| 5832 | | > show #!19 models |
| 5833 | | |
| 5834 | | > save P4_J169_class_0_sharp.png supersample 3 transparentBackground true |
| 5835 | | |
| 5836 | | > hide #!19 models |
| 5837 | | |
| 5838 | | > show #!20 models |
| 5839 | | |
| 5840 | | > save P4_J169_class_1_sharp.png supersample 3 transparentBackground true |
| 5841 | | |
| 5842 | | > hide #!20 models |
| 5843 | | |
| 5844 | | > show #!21 models |
| 5845 | | |
| 5846 | | > save P4_J169_class_2_sharp.png supersample 3 transparentBackground true |
| 5847 | | |
| 5848 | | > hide #!21 models |
| 5849 | | |
| 5850 | | > show #!22 models |
| 5851 | | |
| 5852 | | > show #!23 models |
| 5853 | | |
| 5854 | | > hide #!22 models |
| 5855 | | |
| 5856 | | > save P4_J169_class_4_sharp.png supersample 3 transparentBackground true |
| 5857 | | |
| 5858 | | > save "/Users/peiyuyu/Documents/Goethe |
| 5859 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 5860 | | > F_B1/20260501_SA-F_B1_workflow.cxs" |
| 5861 | | |
| 5862 | | > open "/Users/peiyuyu/Documents/Goethe |
| 5863 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 5864 | | > F_B1/cryosparc_P4_J176_002_volume_map_sharp.mrc" |
| 5865 | | |
| 5866 | | Opened cryosparc_P4_J176_002_volume_map_sharp.mrc as #24, grid size 80,80,80, |
| 5867 | | pixel 3.32, shown at level 1.05, step 1, values float32 |
| 5868 | | |
| 5869 | | > show #!22 models |
| 5870 | | |
| 5871 | | > hide #!23 models |
| 5872 | | |
| 5873 | | > select add #24 |
| 5874 | | |
| 5875 | | 2 models selected |
| 5876 | | |
| 5877 | | > ui mousemode right "rotate selected models" |
| 5878 | | |
| 5879 | | > view matrix models |
| 5880 | | > #24,0.60544,-0.7944,-0.048687,168.77,-0.79404,-0.60707,0.031193,325.29,-0.054337,0.019774,-0.99833,244.32 |
| 5881 | | |
| 5882 | | > view matrix models |
| 5883 | | > #24,0.59955,-0.80023,-0.012763,166.07,-0.79939,-0.59954,0.03913,324.05,-0.038965,-0.013258,-0.99915,246.8 |
| 5884 | | |
| 5885 | | > view matrix models |
| 5886 | | > #24,0.55506,-0.74882,-0.36218,207.08,-0.8209,-0.56341,-0.093219,337.96,-0.13425,0.34906,-0.92744,201.93 |
| 5887 | | |
| 5888 | | > view matrix models |
| 5889 | | > #24,0.18767,0.98161,-0.034938,-17.516,0.95411,-0.19063,-0.23093,58.314,-0.23335,0.010004,-0.97234,267.28 |
| 5890 | | |
| 5891 | | > volume flip #24 |
| 5892 | | |
| 5893 | | Opened cryosparc_P4_J176_002_volume_map_sharp.mrc z flip as #25, grid size |
| 5894 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 5895 | | |
| 5896 | | > close #24 |
| 5897 | | |
| 5898 | | > select add #25 |
| 5899 | | |
| 5900 | | 2 models selected |
| 5901 | | |
| 5902 | | > view matrix models |
| 5903 | | > #25,-0.63444,0.77032,-0.064025,129.07,-0.77136,-0.63629,-0.011971,326.31,-0.04996,0.041792,0.99788,-43.633 |
| 5904 | | |
| 5905 | | > ui mousemode right "translate selected models" |
| 5906 | | |
| 5907 | | > view matrix models |
| 5908 | | > #25,-0.63444,0.77032,-0.064025,133.77,-0.77136,-0.63629,-0.011971,326.45,-0.04996,0.041792,0.99788,5.2846 |
| 5909 | | |
| 5910 | | > fitmap #25 inMap #22 |
| 5911 | | |
| 5912 | | Fit map cryosparc_P4_J176_002_volume_map_sharp.mrc z flip in map |
| 5913 | | CS_P4_J169_class_03_sharp_flip.mrc using 5115 points |
| 5914 | | correlation = 0.927, correlation about mean = 0.5943, overlap = 3935 |
| 5915 | | steps = 92, shift = 11.4, angle = 9.34 degrees |
| 5916 | | |
| 5917 | | Position of cryosparc_P4_J176_002_volume_map_sharp.mrc z flip (#25) relative |
| 5918 | | to CS_P4_J169_class_03_sharp_flip.mrc (#22) coordinates: |
| 5919 | | Matrix rotation and translation |
| 5920 | | -0.48979714 -0.86290735 -0.12445753 333.02911722 |
| 5921 | | 0.86312907 -0.50006428 0.07031310 74.16708713 |
| 5922 | | -0.12291046 -0.07298376 0.98973047 29.10111268 |
| 5923 | | Axis -0.08273609 -0.00089324 0.99657109 |
| 5924 | | Axis point 146.24021491 133.58927656 0.00000000 |
| 5925 | | Rotation angle (degrees) 120.00433179 |
| 5926 | | Shift along axis 1.38155071 |
| 5927 | | |
| 5928 | | |
| 5929 | | > select subtract #25 |
| 5930 | | |
| 5931 | | Nothing selected |
| 5932 | | |
| 5933 | | > rename #25 CS_P4_J176_NU_sharp_flip id #24 |
| 5934 | | |
| 5935 | | > save CS_P4_J176_NU_sharp_flip.mrc #24 |
| 5936 | | |
| 5937 | | > hide #!22 models |
| 5938 | | |
| 5939 | | > volume #24 level 1.757 |
| 5940 | | |
| 5941 | | > volume #24 level 1.364 |
| 5942 | | |
| 5943 | | > color #24 #99BFE5 |
| 5944 | | |
| 5945 | | > volume #24 level 1.6 |
| 5946 | | |
| 5947 | | > save "/Users/peiyuyu/Documents/Goethe |
| 5948 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 5949 | | > F_B1/20260501_SA-F_B1_workflow.cxs" |
| 5950 | | |
| 5951 | | > view P1 |
| 5952 | | |
| 5953 | | > save CS_P4_J176_NU_sharp.png supersample 3 transparentBackground true |
| 5954 | | |
| 5955 | | > open "/Users/peiyuyu/Documents/Goethe University/19. Model Building/2. AcrF |
| 5956 | | > Project/1. Apo-form/LG-F_Apo/Map/cryosparc_P10_J41_007_volume_map_sharp.mrc" |
| 5957 | | |
| 5958 | | Opened cryosparc_P10_J41_007_volume_map_sharp.mrc as #25, grid size |
| 5959 | | 320,320,320, pixel 0.831, shown at level 0.123, step 2, values float32 |
| 5960 | | |
| 5961 | | > hide #!24 models |
| 5962 | | |
| 5963 | | > show #!22 models |
| 5964 | | |
| 5965 | | > hide #!22 models |
| 5966 | | |
| 5967 | | > volume #25 level 0.2164 |
| 5968 | | |
| 5969 | | > save CS_P10_J41_NU_sharp_flip.mrc #25 |
| 5970 | | |
| 5971 | | > close #25 |
| 5972 | | |
| 5973 | | > show #!24 models |
| 5974 | | |
| 5975 | | > save "/Users/peiyuyu/Documents/Goethe |
| 5976 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 5977 | | > F_B1/20260501_SA-F_B1_workflow1.cxs" |
| 5978 | | |
| 5979 | | > open "/Users/peiyuyu/Documents/Goethe |
| 5980 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 5981 | | > F_B1/cryosparc_P4_J255_002_volume_map_sharp.mrc" |
| 5982 | | |
| 5983 | | Opened cryosparc_P4_J255_002_volume_map_sharp.mrc as #25, grid size 80,80,80, |
| 5984 | | pixel 3.32, shown at level 0.649, step 1, values float32 |
| 5985 | | |
| 5986 | | > hide #!24 models |
| 5987 | | |
| 5988 | | > volume flip #25 |
| 5989 | | |
| 5990 | | Opened cryosparc_P4_J255_002_volume_map_sharp.mrc z flip as #26, grid size |
| 5991 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 5992 | | |
| 5993 | | > close #25 |
| 5994 | | |
| 5995 | | > rename CS_P4_J255_sharp_flip id #25 |
| 5996 | | |
| 5997 | | Missing or invalid "models" argument: invalid models specifier |
| 5998 | | |
| 5999 | | > rename #26 CS_P4_J255_sharp_flip id #25 |
| 6000 | | |
| 6001 | | > save CS_P4_J255_sharp_flip.mrc #25 |
| 6002 | | |
| 6003 | | > show #!21 models |
| 6004 | | |
| 6005 | | > hide #!21 models |
| 6006 | | |
| 6007 | | > show #!24 models |
| 6008 | | |
| 6009 | | > fitmap #25 inMap #24 |
| 6010 | | |
| 6011 | | Fit map CS_P4_J255_sharp_flip.mrc in map CS_P4_J176_NU_sharp_flip.mrc using |
| 6012 | | 5116 points |
| 6013 | | correlation = 0.3392, correlation about mean = 0.06206, overlap = 2168 |
| 6014 | | steps = 64, shift = 8.32, angle = 5.45 degrees |
| 6015 | | |
| 6016 | | Position of CS_P4_J255_sharp_flip.mrc (#25) relative to |
| 6017 | | CS_P4_J176_NU_sharp_flip.mrc (#24) coordinates: |
| 6018 | | Matrix rotation and translation |
| 6019 | | -0.61720035 -0.75989860 -0.20400451 353.05950420 |
| 6020 | | 0.73150715 -0.64968488 0.20689815 96.93395715 |
| 6021 | | -0.28976026 -0.02153315 0.95685700 43.12583281 |
| 6022 | | Axis -0.15115555 0.05674554 0.98687990 |
| 6023 | | Axis point 157.34041998 128.70985965 0.00000000 |
| 6024 | | Rotation angle (degrees) 130.92072050 |
| 6025 | | Shift along axis -5.30631665 |
| 6026 | | |
| 6027 | | |
| 6028 | | > ui mousemode right "rotate selected models" |
| 6029 | | |
| 6030 | | > select add #25 |
| 6031 | | |
| 6032 | | 2 models selected |
| 6033 | | |
| 6034 | | > view matrix models |
| 6035 | | > #25,-0.47042,-0.83357,-0.28961,363.38,0.84754,-0.51818,0.11478,76.044,-0.24575,-0.19146,0.95024,72.926 |
| 6036 | | |
| 6037 | | > ui mousemode right "translate selected models" |
| 6038 | | |
| 6039 | | > view matrix models |
| 6040 | | > #25,-0.47042,-0.83357,-0.28961,355.09,0.84754,-0.51818,0.11478,66.81,-0.24575,-0.19146,0.95024,74.95 |
| 6041 | | |
| 6042 | | > view matrix models |
| 6043 | | > #25,-0.47042,-0.83357,-0.28961,355.15,0.84754,-0.51818,0.11478,66.803,-0.24575,-0.19146,0.95024,74.561 |
| 6044 | | |
| 6045 | | > fitmap #25 inMap #24 |
| 6046 | | |
| 6047 | | Fit map CS_P4_J255_sharp_flip.mrc in map CS_P4_J176_NU_sharp_flip.mrc using |
| 6048 | | 5116 points |
| 6049 | | correlation = 0.8943, correlation about mean = 0.6059, overlap = 8709 |
| 6050 | | steps = 84, shift = 8.95, angle = 14.5 degrees |
| 6051 | | |
| 6052 | | Position of CS_P4_J255_sharp_flip.mrc (#25) relative to |
| 6053 | | CS_P4_J176_NU_sharp_flip.mrc (#24) coordinates: |
| 6054 | | Matrix rotation and translation |
| 6055 | | -0.48869161 0.86324945 -0.12641554 102.63693670 |
| 6056 | | -0.86157381 -0.50031742 -0.08586638 328.42188396 |
| 6057 | | -0.13737200 0.06695414 0.98825405 11.50507804 |
| 6058 | | Axis 0.08825319 0.00632731 -0.99607798 |
| 6059 | | Axis point 146.96136624 134.50878130 0.00000000 |
| 6060 | | Rotation angle (degrees) 120.02497766 |
| 6061 | | Shift along axis -0.32389146 |
| 6062 | | |
| 6063 | | |
| 6064 | | > select subtract #25 |
| 6065 | | |
| 6066 | | Nothing selected |
| 6067 | | |
| 6068 | | > save CS_P4_J255_sharp_flip.mrc #25 |
| 6069 | | |
| 6070 | | > open "/Users/peiyuyu/Documents/Goethe |
| 6071 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 6072 | | > F_B1/cryosparc_P4_J256_003_volume_map_sharp.mrc" |
| 6073 | | |
| 6074 | | Opened cryosparc_P4_J256_003_volume_map_sharp.mrc as #26, grid size 80,80,80, |
| 6075 | | pixel 3.32, shown at level 1.01, step 1, values float32 |
| 6076 | | |
| 6077 | | > hide #!25 models |
| 6078 | | |
| 6079 | | > hide #!24 models |
| 6080 | | |
| 6081 | | > select add #26 |
| 6082 | | |
| 6083 | | 2 models selected |
| 6084 | | |
| 6085 | | > ui mousemode right "rotate selected models" |
| 6086 | | |
| 6087 | | > view matrix models |
| 6088 | | > #26,-0.89385,-0.39986,0.20284,290.33,-0.34063,0.89978,0.2727,28.49,-0.29155,0.17466,-0.94047,242.77 |
| 6089 | | |
| 6090 | | > select subtract #26 |
| 6091 | | |
| 6092 | | Nothing selected |
| 6093 | | |
| 6094 | | > show #!25 models |
| 6095 | | |
| 6096 | | > fitmap #26 inMap #25 |
| 6097 | | |
| 6098 | | Fit map cryosparc_P4_J256_003_volume_map_sharp.mrc in map |
| 6099 | | CS_P4_J255_sharp_flip.mrc using 5120 points |
| 6100 | | correlation = 0.5232, correlation about mean = 0.0553, overlap = 3214 |
| 6101 | | steps = 120, shift = 25.3, angle = 15.3 degrees |
| 6102 | | |
| 6103 | | Position of cryosparc_P4_J256_003_volume_map_sharp.mrc (#26) relative to |
| 6104 | | CS_P4_J255_sharp_flip.mrc (#25) coordinates: |
| 6105 | | Matrix rotation and translation |
| 6106 | | -0.15531756 0.84438274 0.51273214 -19.64184672 |
| 6107 | | 0.98713928 0.15254692 0.04780678 -29.86548711 |
| 6108 | | -0.03784849 0.51356326 -0.85721658 167.46829582 |
| 6109 | | Axis 0.63355180 0.74893504 0.19418658 |
| 6110 | | Axis point 24.16142457 0.00000000 89.91884511 |
| 6111 | | Rotation angle (degrees) 158.43381860 |
| 6112 | | Shift along axis -2.29134077 |
| 6113 | | |
| 6114 | | |
| 6115 | | > select add #26 |
| 6116 | | |
| 6117 | | 2 models selected |
| 6118 | | |
| 6119 | | > ui mousemode right "translate selected models" |
| 6120 | | |
| 6121 | | > view matrix models |
| 6122 | | > #26,-0.87857,-0.47692,-0.025855,314.85,-0.44818,0.80449,0.38978,38.161,-0.16509,0.35404,-0.92054,233.61 |
| 6123 | | |
| 6124 | | > ui mousemode right "rotate selected models" |
| 6125 | | |
| 6126 | | > view matrix models |
| 6127 | | > #26,-0.93629,-0.25688,0.23952,260.92,-0.23661,0.96532,0.11035,19.606,-0.25956,0.046645,-0.9646,294.56 |
| 6128 | | |
| 6129 | | > ui mousemode right "translate selected models" |
| 6130 | | |
| 6131 | | > view matrix models |
| 6132 | | > #26,-0.93629,-0.25688,0.23952,186.31,-0.23661,0.96532,0.11035,26.555,-0.25956,0.046645,-0.9646,285.53 |
| 6133 | | |
| 6134 | | > select subtract #26 |
| 6135 | | |
| 6136 | | Nothing selected |
| 6137 | | |
| 6138 | | > volume flip #26 |
| 6139 | | |
| 6140 | | Opened cryosparc_P4_J256_003_volume_map_sharp.mrc z flip as #27, grid size |
| 6141 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 6142 | | |
| 6143 | | > close #26 |
| 6144 | | |
| 6145 | | > select add #27 |
| 6146 | | |
| 6147 | | 2 models selected |
| 6148 | | |
| 6149 | | > ui mousemode right "rotate selected models" |
| 6150 | | |
| 6151 | | > view matrix models |
| 6152 | | > #27,-0.91261,-0.40844,0.017647,236.5,0.22697,-0.47029,0.85283,55.691,-0.34003,0.78231,0.5219,-22.328 |
| 6153 | | |
| 6154 | | > view matrix models |
| 6155 | | > #27,-0.9144,-0.40478,0.0045882,238.13,0.22235,-0.49276,0.84128,61.136,-0.33827,0.77029,0.54058,-23.608 |
| 6156 | | |
| 6157 | | > view matrix models |
| 6158 | | > #27,-0.50015,0.79795,-0.33634,63.369,-0.80597,-0.57099,-0.15615,357.35,-0.31664,0.19298,0.92871,-2.4372 |
| 6159 | | |
| 6160 | | > ui mousemode right "translate selected models" |
| 6161 | | |
| 6162 | | > view matrix models |
| 6163 | | > #27,-0.50015,0.79795,-0.33634,139.66,-0.80597,-0.57099,-0.15615,346.55,-0.31664,0.19298,0.92871,33.757 |
| 6164 | | |
| 6165 | | > fitmap #27 inMap #25 |
| 6166 | | |
| 6167 | | Fit map cryosparc_P4_J256_003_volume_map_sharp.mrc z flip in map |
| 6168 | | CS_P4_J255_sharp_flip.mrc using 5120 points |
| 6169 | | correlation = 0.5523, correlation about mean = 0.08213, overlap = 3668 |
| 6170 | | steps = 72, shift = 2.85, angle = 5.49 degrees |
| 6171 | | |
| 6172 | | Position of cryosparc_P4_J256_003_volume_map_sharp.mrc z flip (#27) relative |
| 6173 | | to CS_P4_J255_sharp_flip.mrc (#25) coordinates: |
| 6174 | | Matrix rotation and translation |
| 6175 | | -0.50078193 -0.83716343 -0.21994286 353.70374510 |
| 6176 | | 0.81104417 -0.54259583 0.21862553 68.19134780 |
| 6177 | | -0.30236538 -0.06889966 0.95069870 54.62167416 |
| 6178 | | Axis -0.17164375 0.04920372 0.98392958 |
| 6179 | | Axis point 162.79794553 129.91438640 0.00000000 |
| 6180 | | Rotation angle (degrees) 123.11624986 |
| 6181 | | Shift along axis -3.61188964 |
| 6182 | | |
| 6183 | | |
| 6184 | | > view matrix models |
| 6185 | | > #27,-0.56613,0.75777,-0.32448,155.66,-0.74832,-0.63753,-0.18323,351.66,-0.34571,0.13908,0.92798,47.028 |
| 6186 | | |
| 6187 | | > ui mousemode right "rotate selected models" |
| 6188 | | |
| 6189 | | > view matrix models |
| 6190 | | > #27,0.96274,0.27031,-0.0085132,-30.789,-0.27044,0.96237,-0.026732,40.459,0.00096683,0.028039,0.99961,4.8234 |
| 6191 | | |
| 6192 | | > fitmap #27 inMap #25 |
| 6193 | | |
| 6194 | | Fit map cryosparc_P4_J256_003_volume_map_sharp.mrc z flip in map |
| 6195 | | CS_P4_J255_sharp_flip.mrc using 5120 points |
| 6196 | | correlation = 0.9111, correlation about mean = 0.5876, overlap = 9195 |
| 6197 | | steps = 60, shift = 4.49, angle = 4.9 degrees |
| 6198 | | |
| 6199 | | Position of cryosparc_P4_J256_003_volume_map_sharp.mrc z flip (#27) relative |
| 6200 | | to CS_P4_J255_sharp_flip.mrc (#25) coordinates: |
| 6201 | | Matrix rotation and translation |
| 6202 | | -0.48877401 0.86149397 -0.13757950 104.57166396 |
| 6203 | | -0.86119668 -0.50165763 -0.08173068 327.97179743 |
| 6204 | | -0.13942829 0.07853517 0.98711295 10.19538079 |
| 6205 | | Axis 0.09263223 0.00106859 -0.99569982 |
| 6206 | | Axis point 147.63593381 133.73777093 0.00000000 |
| 6207 | | Rotation angle (degrees) 120.10984192 |
| 6208 | | Shift along axis -0.11436514 |
| 6209 | | |
| 6210 | | |
| 6211 | | > select subtract #27 |
| 6212 | | |
| 6213 | | Nothing selected |
| 6214 | | |
| 6215 | | > hide #!25 models |
| 6216 | | |
| 6217 | | > rename #27 CS_P4_J256_sharp_flip id #26 |
| 6218 | | |
| 6219 | | > save CS_P4_J256_sharp_flip.mrc #26 |
| 6220 | | |
| 6221 | | > show #!25 models |
| 6222 | | |
| 6223 | | > hide #!25 models |
| 6224 | | |
| 6225 | | > hide #!26 models |
| 6226 | | |
| 6227 | | > open "/Users/peiyuyu/Documents/Goethe |
| 6228 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 6229 | | > F_B1/cryosparc_P4_J257_002_volume_map_sharp.mrc" |
| 6230 | | |
| 6231 | | Opened cryosparc_P4_J257_002_volume_map_sharp.mrc as #27, grid size 80,80,80, |
| 6232 | | pixel 3.32, shown at level 0.915, step 1, values float32 |
| 6233 | | |
| 6234 | | > select add #27 |
| 6235 | | |
| 6236 | | 2 models selected |
| 6237 | | |
| 6238 | | > show #!26 models |
| 6239 | | |
| 6240 | | > view matrix models |
| 6241 | | > #27,0.43348,0.88304,0.17982,-64.533,0.89699,-0.44198,0.0081618,72.887,0.086685,0.15776,-0.98367,205.49 |
| 6242 | | |
| 6243 | | > hide #!26 models |
| 6244 | | |
| 6245 | | > select subtract #27 |
| 6246 | | |
| 6247 | | Nothing selected |
| 6248 | | |
| 6249 | | > volume flip #27 |
| 6250 | | |
| 6251 | | Opened cryosparc_P4_J257_002_volume_map_sharp.mrc z flip as #28, grid size |
| 6252 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 6253 | | |
| 6254 | | > close #27 |
| 6255 | | |
| 6256 | | > select add #28 |
| 6257 | | |
| 6258 | | 2 models selected |
| 6259 | | |
| 6260 | | > show #!26 models |
| 6261 | | |
| 6262 | | > view matrix models |
| 6263 | | > #28,0.0384,0.0015757,0.99926,-5.8546,0.92609,-0.37568,-0.034996,65.933,0.37535,0.92675,-0.015886,-77.078 |
| 6264 | | |
| 6265 | | > view matrix models |
| 6266 | | > #28,0.75378,0.65514,-0.051086,-44.642,-0.54582,0.58092,-0.60384,218.55,-0.36592,0.48304,0.79547,-29.499 |
| 6267 | | |
| 6268 | | > view matrix models |
| 6269 | | > #28,0.93053,0.32568,-0.16747,-7.3436,-0.34314,0.93516,-0.087963,68.823,0.12796,0.13932,0.98195,-76.969 |
| 6270 | | |
| 6271 | | > ui mousemode right "translate selected models" |
| 6272 | | |
| 6273 | | > view matrix models |
| 6274 | | > #28,0.93053,0.32568,-0.16747,-12.031,-0.34314,0.93516,-0.087963,69.72,0.12796,0.13932,0.98195,-23.264 |
| 6275 | | |
| 6276 | | > select subtract #28 |
| 6277 | | |
| 6278 | | Nothing selected |
| 6279 | | |
| 6280 | | > fitmap #28 inMap #26 |
| 6281 | | |
| 6282 | | Fit map cryosparc_P4_J257_002_volume_map_sharp.mrc z flip in map |
| 6283 | | CS_P4_J256_sharp_flip.mrc using 5113 points |
| 6284 | | correlation = 0.961, correlation about mean = 0.8355, overlap = 1.813e+04 |
| 6285 | | steps = 76, shift = 3.44, angle = 10.7 degrees |
| 6286 | | |
| 6287 | | Position of cryosparc_P4_J257_002_volume_map_sharp.mrc z flip (#28) relative |
| 6288 | | to CS_P4_J256_sharp_flip.mrc (#26) coordinates: |
| 6289 | | Matrix rotation and translation |
| 6290 | | 0.99983973 0.00266136 0.01770477 -3.60430289 |
| 6291 | | -0.00275366 0.99998275 0.00519130 -0.72993632 |
| 6292 | | -0.01769065 -0.00523922 0.99982979 3.00234542 |
| 6293 | | Axis -0.27967225 0.94905354 -0.14519233 |
| 6294 | | Axis point 166.73187411 0.00000000 206.99324341 |
| 6295 | | Rotation angle (degrees) 1.06849905 |
| 6296 | | Shift along axis -0.12064266 |
| 6297 | | |
| 6298 | | |
| 6299 | | > rename #28 CS_P4_J257_sharp_flip id #27 |
| 6300 | | |
| 6301 | | > save CS_P4_J257_sharp_flip.mrc #27 |
| 6302 | | |
| 6303 | | > hide #!26 models |
| 6304 | | |
| 6305 | | > hide #!27 models |
| 6306 | | |
| 6307 | | > open "/Users/peiyuyu/Documents/Goethe |
| 6308 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 6309 | | > F_B1/cryosparc_P4_J258_002_volume_map_sharp.mrc" |
| 6310 | | |
| 6311 | | Opened cryosparc_P4_J258_002_volume_map_sharp.mrc as #28, grid size 80,80,80, |
| 6312 | | pixel 3.32, shown at level 0.533, step 1, values float32 |
| 6313 | | |
| 6314 | | > select add #28 |
| 6315 | | |
| 6316 | | 2 models selected |
| 6317 | | |
| 6318 | | > show #!27 models |
| 6319 | | |
| 6320 | | > view matrix models #28,1,0,0,2.9293,0,1,0,12.799,0,0,1,30.196 |
| 6321 | | |
| 6322 | | > ui mousemode right "rotate selected models" |
| 6323 | | |
| 6324 | | > view matrix models |
| 6325 | | > #28,-0.94564,-0.32466,0.018849,316.84,-0.32175,0.94244,0.090998,54.708,-0.047307,0.079987,-0.99567,265.03 |
| 6326 | | |
| 6327 | | > select up |
| 6328 | | |
| 6329 | | 56 models selected |
| 6330 | | |
| 6331 | | > select up |
| 6332 | | |
| 6333 | | 56 models selected |
| 6334 | | |
| 6335 | | > select down |
| 6336 | | |
| 6337 | | 2 models selected |
| 6338 | | |
| 6339 | | > select down |
| 6340 | | |
| 6341 | | 2 models selected |
| 6342 | | |
| 6343 | | > select subtract #28 |
| 6344 | | |
| 6345 | | Nothing selected |
| 6346 | | |
| 6347 | | > hide #!27 models |
| 6348 | | |
| 6349 | | > volume flip #28 |
| 6350 | | |
| 6351 | | Opened cryosparc_P4_J258_002_volume_map_sharp.mrc z flip as #29, grid size |
| 6352 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 6353 | | |
| 6354 | | > close #28 |
| 6355 | | |
| 6356 | | > select add #29 |
| 6357 | | |
| 6358 | | 2 models selected |
| 6359 | | |
| 6360 | | > view matrix models |
| 6361 | | > #29,0.95822,0.27409,-0.081793,-16.892,-0.27702,0.96049,-0.026814,62.796,0.071212,0.048352,0.99629,-31.428 |
| 6362 | | |
| 6363 | | > show #!27 models |
| 6364 | | |
| 6365 | | > select subtract #29 |
| 6366 | | |
| 6367 | | Nothing selected |
| 6368 | | |
| 6369 | | > fitmap #29 inMap #27 |
| 6370 | | |
| 6371 | | Fit map cryosparc_P4_J258_002_volume_map_sharp.mrc z flip in map |
| 6372 | | CS_P4_J257_sharp_flip.mrc using 5117 points |
| 6373 | | correlation = 0.4043, correlation about mean = 0.1105, overlap = 1145 |
| 6374 | | steps = 104, shift = 9.56, angle = 9.45 degrees |
| 6375 | | |
| 6376 | | Position of cryosparc_P4_J258_002_volume_map_sharp.mrc z flip (#29) relative |
| 6377 | | to CS_P4_J257_sharp_flip.mrc (#27) coordinates: |
| 6378 | | Matrix rotation and translation |
| 6379 | | 0.98218261 0.18784329 -0.00567361 -27.82492616 |
| 6380 | | -0.18685004 0.97932905 0.07747035 28.59524537 |
| 6381 | | 0.02010861 -0.07502992 0.99697851 -24.36927466 |
| 6382 | | Axis -0.37621012 -0.06360338 -0.92434872 |
| 6383 | | Axis point 152.56947582 100.16313614 0.00000000 |
| 6384 | | Rotation angle (degrees) 11.69370116 |
| 6385 | | Shift along axis 31.17497259 |
| 6386 | | |
| 6387 | | |
| 6388 | | > select add #29 |
| 6389 | | |
| 6390 | | 2 models selected |
| 6391 | | |
| 6392 | | > ui mousemode right "translate selected models" |
| 6393 | | |
| 6394 | | > view matrix models |
| 6395 | | > #29,0.91472,0.40193,-0.041674,-52.044,-0.39883,0.91458,0.066882,60.074,0.064997,-0.044558,0.99689,0.70588 |
| 6396 | | |
| 6397 | | > view matrix models |
| 6398 | | > #29,0.91472,0.40193,-0.041674,-39.705,-0.39883,0.91458,0.066882,54.066,0.064997,-0.044558,0.99689,6.2725 |
| 6399 | | |
| 6400 | | > fitmap #29 inMap #27 |
| 6401 | | |
| 6402 | | Fit map cryosparc_P4_J258_002_volume_map_sharp.mrc z flip in map |
| 6403 | | CS_P4_J257_sharp_flip.mrc using 5117 points |
| 6404 | | correlation = 0.8335, correlation about mean = 0.5457, overlap = 5135 |
| 6405 | | steps = 64, shift = 1.52, angle = 12.1 degrees |
| 6406 | | |
| 6407 | | Position of cryosparc_P4_J258_002_volume_map_sharp.mrc z flip (#29) relative |
| 6408 | | to CS_P4_J257_sharp_flip.mrc (#27) coordinates: |
| 6409 | | Matrix rotation and translation |
| 6410 | | 0.99996433 -0.00752131 -0.00383843 1.74823892 |
| 6411 | | 0.00752090 0.99997170 -0.00012121 -0.88867123 |
| 6412 | | 0.00383923 0.00009234 0.99999262 -0.68815928 |
| 6413 | | Axis 0.01264381 -0.45457780 0.89061729 |
| 6414 | | Axis point 130.32470586 232.13443622 0.00000000 |
| 6415 | | Rotation angle (degrees) 0.48385831 |
| 6416 | | Shift along axis -0.18681194 |
| 6417 | | |
| 6418 | | |
| 6419 | | > select subtract #29 |
| 6420 | | |
| 6421 | | Nothing selected |
| 6422 | | |
| 6423 | | > rename #29 CS_P4_J258_sharp_flip id #28 |
| 6424 | | |
| 6425 | | > save CS_P4_J258_sharp_flip.mrc #28 |
| 6426 | | |
| 6427 | | > hide #!27 models |
| 6428 | | |
| 6429 | | > hide #!28 models |
| 6430 | | |
| 6431 | | > open "/Users/peiyuyu/Documents/Goethe |
| 6432 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 6433 | | > F_B1/cryosparc_P4_J259_002_volume_map_sharp.mrc" |
| 6434 | | |
| 6435 | | Opened cryosparc_P4_J259_002_volume_map_sharp.mrc as #29, grid size 80,80,80, |
| 6436 | | pixel 3.32, shown at level 0.567, step 1, values float32 |
| 6437 | | |
| 6438 | | > select add #29 |
| 6439 | | |
| 6440 | | 2 models selected |
| 6441 | | |
| 6442 | | > show #!28 models |
| 6443 | | |
| 6444 | | > view matrix models #29,1,0,0,-4.0284,0,1,0,3.9973,0,0,1,26.374 |
| 6445 | | |
| 6446 | | > ui mousemode right "rotate selected models" |
| 6447 | | |
| 6448 | | > view matrix models |
| 6449 | | > #29,0.37373,0.91624,0.14434,-63.019,0.92743,-0.36674,-0.073343,74.867,-0.014266,0.16128,-0.98681,258.72 |
| 6450 | | |
| 6451 | | > view matrix models |
| 6452 | | > #29,0.2129,0.93735,0.27578,-60.698,0.97539,-0.1873,-0.11636,49.108,-0.057419,0.29377,-0.95415,242.23 |
| 6453 | | |
| 6454 | | > hide #!28 models |
| 6455 | | |
| 6456 | | > select subtract #29 |
| 6457 | | |
| 6458 | | Nothing selected |
| 6459 | | |
| 6460 | | > volume flip #29 |
| 6461 | | |
| 6462 | | Opened cryosparc_P4_J259_002_volume_map_sharp.mrc z flip as #30, grid size |
| 6463 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 6464 | | |
| 6465 | | > close #29 |
| 6466 | | |
| 6467 | | > select add #30 |
| 6468 | | |
| 6469 | | 2 models selected |
| 6470 | | |
| 6471 | | > show #!28 models |
| 6472 | | |
| 6473 | | > view matrix models |
| 6474 | | > #30,0.90176,0.31537,-0.29559,8.3185,-0.32745,0.94483,0.0091223,54.046,0.28216,0.088565,0.95527,-34.506 |
| 6475 | | |
| 6476 | | > select subtract #30 |
| 6477 | | |
| 6478 | | Nothing selected |
| 6479 | | |
| 6480 | | > fitmap #30 inMap #28 |
| 6481 | | |
| 6482 | | Fit map cryosparc_P4_J259_002_volume_map_sharp.mrc z flip in map |
| 6483 | | CS_P4_J258_sharp_flip.mrc using 5112 points |
| 6484 | | correlation = 0.9666, correlation about mean = 0.6628, overlap = 3020 |
| 6485 | | steps = 80, shift = 5.99, angle = 15.1 degrees |
| 6486 | | |
| 6487 | | Position of cryosparc_P4_J259_002_volume_map_sharp.mrc z flip (#30) relative |
| 6488 | | to CS_P4_J258_sharp_flip.mrc (#28) coordinates: |
| 6489 | | Matrix rotation and translation |
| 6490 | | 0.99996699 0.00262374 -0.00768921 0.94371527 |
| 6491 | | -0.00261765 0.99999625 0.00080251 0.08556771 |
| 6492 | | 0.00769129 -0.00078235 0.99997011 -0.47262490 |
| 6493 | | Axis -0.09707497 -0.94207663 -0.32104217 |
| 6494 | | Axis point 62.77947672 0.00000000 122.20423531 |
| 6495 | | Rotation angle (degrees) 0.46771540 |
| 6496 | | Shift along axis -0.02048995 |
| 6497 | | |
| 6498 | | |
| 6499 | | > rename #30 CS_P4_J259_sharp_flip id #29 |
| 6500 | | |
| 6501 | | > save CS_P4_J259_sharp_flip.mrc #29 |
| 6502 | | |
| 6503 | | > save "/Users/peiyuyu/Documents/Goethe |
| 6504 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 6505 | | > F_B1/20260502_SA-F_B1_workflow.cxs" |
| 6506 | | |
| 6507 | | > hide #!29 models |
| 6508 | | |
| 6509 | | > hide #!28 models |
| 6510 | | |
| 6511 | | > show #!25 models |
| 6512 | | |
| 6513 | | > color #25 #FCEDFF |
| 6514 | | |
| 6515 | | > hide #!25 models |
| 6516 | | |
| 6517 | | > show #!28 models |
| 6518 | | |
| 6519 | | > color #28 #FCEDFF |
| 6520 | | |
| 6521 | | > hide #!28 models |
| 6522 | | |
| 6523 | | > show #!29 models |
| 6524 | | |
| 6525 | | > color #29 #FCEDFF |
| 6526 | | |
| 6527 | | > hide #!29 models |
| 6528 | | |
| 6529 | | > show #!29 models |
| 6530 | | |
| 6531 | | > show #!26 models |
| 6532 | | |
| 6533 | | > hide #!29 models |
| 6534 | | |
| 6535 | | > color #26 #99BFE5 |
| 6536 | | |
| 6537 | | > hide #!26 models |
| 6538 | | |
| 6539 | | > show #!27 models |
| 6540 | | |
| 6541 | | > color #27 #99BFE5 |
| 6542 | | |
| 6543 | | > view P1 |
| 6544 | | |
| 6545 | | > hide #!27 models |
| 6546 | | |
| 6547 | | > show #!25 models |
| 6548 | | |
| 6549 | | > ui mousemode right "translate selected models" |
| 6550 | | |
| 6551 | | > ui mousemode right translate |
| 6552 | | |
| 6553 | | > view P1 |
| 6554 | | |
| 6555 | | > save CS_P4_J255_NU_sharp.png supersample 3 transparentBackground true |
| 6556 | | |
| 6557 | | > hide #!25 models |
| 6558 | | |
| 6559 | | > show #!26 models |
| 6560 | | |
| 6561 | | > volume #26 level 1.012 |
| 6562 | | |
| 6563 | | > save CS_P4_J256_NU_sharp.png supersample 3 transparentBackground true |
| 6564 | | |
| 6565 | | > hide #!26 models |
| 6566 | | |
| 6567 | | > show #!27 models |
| 6568 | | |
| 6569 | | > save CS_P4_J257_NU_sharp.png supersample 3 transparentBackground true |
| 6570 | | |
| 6571 | | > hide #!27 models |
| 6572 | | |
| 6573 | | > show #!28 models |
| 6574 | | |
| 6575 | | > save CS_P4_J258_NU_sharp.png supersample 3 transparentBackground true |
| 6576 | | |
| 6577 | | > hide #!28 models |
| 6578 | | |
| 6579 | | > show #!29 models |
| 6580 | | |
| 6581 | | > save CS_P4_J259_NU_sharp.png supersample 3 transparentBackground true |
| 6582 | | |
| 6583 | | > save "/Users/peiyuyu/Documents/Goethe |
| 6584 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 6585 | | > F_B1/20260502_SA-F_B1_workflow.cxs" |
| 6586 | | |
| 6587 | | > open "/Users/peiyuyu/Documents/Goethe |
| 6588 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 6589 | | > F_B1/cryosparc_P4_J285_006_volume_map_sharp.mrc" |
| 6590 | | |
| 6591 | | Opened cryosparc_P4_J285_006_volume_map_sharp.mrc as #30, grid size |
| 6592 | | 320,320,320, pixel 0.831, shown at level 0.0363, step 2, values float32 |
| 6593 | | |
| 6594 | | > open "/Users/peiyuyu/Documents/Goethe |
| 6595 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 6596 | | > F_B1/cryosparc_P4_J267_008_volume_map_sharp.mrc" |
| 6597 | | |
| 6598 | | Opened cryosparc_P4_J267_008_volume_map_sharp.mrc as #31, grid size |
| 6599 | | 320,320,320, pixel 0.831, shown at level 0.0747, step 2, values float32 |
| 6600 | | |
| 6601 | | > open "/Users/peiyuyu/Documents/Goethe |
| 6602 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 6603 | | > F_B1/cryosparc_P4_J277_008_volume_map_sharp.mrc" |
| 6604 | | |
| 6605 | | Opened cryosparc_P4_J277_008_volume_map_sharp.mrc as #32, grid size |
| 6606 | | 320,320,320, pixel 0.831, shown at level 0.0762, step 2, values float32 |
| 6607 | | |
| 6608 | | > hide #!29 models |
| 6609 | | |
| 6610 | | > hide #!30 models |
| 6611 | | |
| 6612 | | > hide #!31 models |
| 6613 | | |
| 6614 | | > show #!31 models |
| 6615 | | |
| 6616 | | > hide #!32 models |
| 6617 | | |
| 6618 | | > show #!30 models |
| 6619 | | |
| 6620 | | > close #30 |
| 6621 | | |
| 6622 | | > show #!27 models |
| 6623 | | |
| 6624 | | > select add #31 |
| 6625 | | |
| 6626 | | 2 models selected |
| 6627 | | |
| 6628 | | > ui mousemode right "translate selected models" |
| 6629 | | |
| 6630 | | > ui mousemode right "rotate selected models" |
| 6631 | | |
| 6632 | | > view matrix models |
| 6633 | | > #31,-0.90396,0.26529,0.33537,196.37,0.29187,0.95597,0.030503,-41.724,-0.31251,0.12546,-0.94159,315.51 |
| 6634 | | |
| 6635 | | > hide #!27 models |
| 6636 | | |
| 6637 | | > view matrix models |
| 6638 | | > #31,-0.95736,-0.095902,0.27253,266.49,-0.06692,0.99125,0.11374,-5.5307,-0.28105,0.090649,-0.9554,317.94 |
| 6639 | | |
| 6640 | | > show #!29 models |
| 6641 | | |
| 6642 | | > hide #!29 models |
| 6643 | | |
| 6644 | | > select subtract #31 |
| 6645 | | |
| 6646 | | Nothing selected |
| 6647 | | |
| 6648 | | > close #32 |
| 6649 | | |
| 6650 | | > volume flip #31 |
| 6651 | | |
| 6652 | | Opened cryosparc_P4_J267_008_volume_map_sharp.mrc z flip as #30, grid size |
| 6653 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 6654 | | |
| 6655 | | > close #30 |
| 6656 | | |
| 6657 | | > show #!31 models |
| 6658 | | |
| 6659 | | > select add #31 |
| 6660 | | |
| 6661 | | 2 models selected |
| 6662 | | |
| 6663 | | > show #!27 models |
| 6664 | | |
| 6665 | | > ui mousemode right "translate selected models" |
| 6666 | | |
| 6667 | | > view matrix models |
| 6668 | | > #31,-0.95736,-0.095902,0.27253,258.1,-0.06692,0.99125,0.11374,14.401,-0.28105,0.090649,-0.9554,295.97 |
| 6669 | | |
| 6670 | | > ui mousemode right "rotate selected models" |
| 6671 | | |
| 6672 | | > view matrix models |
| 6673 | | > #31,0.57983,0.79903,0.15922,-85.975,0.81408,-0.57604,-0.073785,139.64,0.032763,0.1724,-0.98448,241.38 |
| 6674 | | |
| 6675 | | > ui mousemode right "translate selected models" |
| 6676 | | |
| 6677 | | > view matrix models |
| 6678 | | > #31,0.57983,0.79903,0.15922,-74.26,0.81408,-0.57604,-0.073785,114.14,0.032763,0.1724,-0.98448,243.66 |
| 6679 | | |
| 6680 | | > ui mousemode right "rotate selected models" |
| 6681 | | |
| 6682 | | > view matrix models |
| 6683 | | > #31,0.38773,0.91358,0.12259,-56.869,0.92165,-0.3821,-0.067497,68.753,-0.014823,0.13915,-0.99016,256.48 |
| 6684 | | |
| 6685 | | > ui mousemode right "translate selected models" |
| 6686 | | |
| 6687 | | > view matrix models |
| 6688 | | > #31,0.38773,0.91358,0.12259,-59.17,0.92165,-0.3821,-0.067497,73.776,-0.014823,0.13915,-0.99016,255.81 |
| 6689 | | |
| 6690 | | > fitmap #31 inMap #27 |
| 6691 | | |
| 6692 | | Fit map cryosparc_P4_J267_008_volume_map_sharp.mrc in map |
| 6693 | | CS_P4_J257_sharp_flip.mrc using 40948 points |
| 6694 | | correlation = 0.4368, correlation about mean = 0.04808, overlap = 2838 |
| 6695 | | steps = 84, shift = 6.03, angle = 16 degrees |
| 6696 | | |
| 6697 | | Position of cryosparc_P4_J267_008_volume_map_sharp.mrc (#31) relative to |
| 6698 | | CS_P4_J257_sharp_flip.mrc (#27) coordinates: |
| 6699 | | Matrix rotation and translation |
| 6700 | | -0.09805932 0.98945486 0.10659935 2.84911711 |
| 6701 | | 0.99008140 0.10782511 -0.09006967 -2.59568012 |
| 6702 | | -0.10061396 0.09670986 -0.99021412 262.50076525 |
| 6703 | | Axis 0.66953234 0.74277951 0.00224590 |
| 6704 | | Axis point 14.97851108 -0.00000000 131.13786872 |
| 6705 | | Rotation angle (degrees) 171.98194915 |
| 6706 | | Shift along axis 0.56910865 |
| 6707 | | |
| 6708 | | |
| 6709 | | > select subtract #31 |
| 6710 | | |
| 6711 | | Nothing selected |
| 6712 | | |
| 6713 | | > hide #!31 models |
| 6714 | | |
| 6715 | | > hide #!27 models |
| 6716 | | |
| 6717 | | > show #!31 models |
| 6718 | | |
| 6719 | | > close #31 |
| 6720 | | |
| 6721 | | > open "/Users/peiyuyu/Documents/Goethe |
| 6722 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 6723 | | > F_B1/cryosparc_P4_J267_008_volume_map_sharp.mrc" |
| 6724 | | |
| 6725 | | Opened cryosparc_P4_J267_008_volume_map_sharp.mrc as #30, grid size |
| 6726 | | 320,320,320, pixel 0.831, shown at level 0.0747, step 2, values float32 |
| 6727 | | |
| 6728 | | > select add #30 |
| 6729 | | |
| 6730 | | 2 models selected |
| 6731 | | |
| 6732 | | > show #!27 models |
| 6733 | | |
| 6734 | | > ui mousemode right "rotate selected models" |
| 6735 | | |
| 6736 | | > view matrix models |
| 6737 | | > #30,0.59059,-0.80637,-0.031153,183.8,-0.80688,-0.59066,-0.008015,354.56,-0.011937,0.02987,-0.99948,293.1 |
| 6738 | | |
| 6739 | | > ui mousemode right "translate selected models" |
| 6740 | | |
| 6741 | | > view matrix models |
| 6742 | | > #30,0.59059,-0.80637,-0.031153,187.84,-0.80688,-0.59066,-0.008015,342.67,-0.011937,0.02987,-0.99948,267.57 |
| 6743 | | |
| 6744 | | > hide #!27 models |
| 6745 | | |
| 6746 | | > show #!27 models |
| 6747 | | |
| 6748 | | > hide #!30 models |
| 6749 | | |
| 6750 | | > hide #!27 models |
| 6751 | | |
| 6752 | | > show #!30 models |
| 6753 | | |
| 6754 | | > volume flip #30 |
| 6755 | | |
| 6756 | | Opened cryosparc_P4_J267_008_volume_map_sharp.mrc z flip as #31, grid size |
| 6757 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 6758 | | |
| 6759 | | > select subtract #30 |
| 6760 | | |
| 6761 | | Nothing selected |
| 6762 | | |
| 6763 | | > close #30 |
| 6764 | | |
| 6765 | | > select add #31 |
| 6766 | | |
| 6767 | | 2 models selected |
| 6768 | | |
| 6769 | | > show #!27 models |
| 6770 | | |
| 6771 | | > view matrix models |
| 6772 | | > #31,0.59059,-0.80637,-0.031153,181.08,-0.80688,-0.59066,-0.008015,342.68,-0.011937,0.02987,-0.99948,285.6 |
| 6773 | | |
| 6774 | | > ui mousemode right "rotate selected models" |
| 6775 | | |
| 6776 | | > view matrix models |
| 6777 | | > #31,0.49825,-0.85813,0.12394,182.63,-0.032454,0.12438,0.9917,19.314,-0.86642,-0.49814,0.034125,354.11 |
| 6778 | | |
| 6779 | | > view matrix models |
| 6780 | | > #31,-0.47795,-0.87619,-0.061991,343.39,0.85267,-0.47976,0.20686,70.492,-0.21099,0.046011,0.9764,77.316 |
| 6781 | | |
| 6782 | | > ui mousemode right "translate selected models" |
| 6783 | | |
| 6784 | | > view matrix models |
| 6785 | | > #31,-0.47795,-0.87619,-0.061991,322.39,0.85267,-0.47976,0.20686,71.691,-0.21099,0.046011,0.9764,36.515 |
| 6786 | | |
| 6787 | | > view matrix models |
| 6788 | | > #31,-0.47795,-0.87619,-0.061991,329.78,0.85267,-0.47976,0.20686,58.248,-0.21099,0.046011,0.9764,36.244 |
| 6789 | | |
| 6790 | | > fitmap #31 inMap #27 |
| 6791 | | |
| 6792 | | Fit map cryosparc_P4_J267_008_volume_map_sharp.mrc z flip in map |
| 6793 | | CS_P4_J257_sharp_flip.mrc using 327854 points |
| 6794 | | correlation = 0.6967, correlation about mean = 0.2684, overlap = 4.49e+04 |
| 6795 | | steps = 92, shift = 8.63, angle = 16.2 degrees |
| 6796 | | |
| 6797 | | Position of cryosparc_P4_J267_008_volume_map_sharp.mrc z flip (#31) relative |
| 6798 | | to CS_P4_J257_sharp_flip.mrc (#27) coordinates: |
| 6799 | | Matrix rotation and translation |
| 6800 | | -0.48810574 -0.85845883 -0.15748400 338.09346066 |
| 6801 | | 0.86132304 -0.50293557 0.07196129 74.97027819 |
| 6802 | | -0.14098011 -0.10051988 0.98489611 32.48936744 |
| 6803 | | Axis -0.09978729 -0.00954816 0.99496298 |
| 6804 | | Axis point 149.01931346 135.27108975 0.00000000 |
| 6805 | | Rotation angle (degrees) 120.20349080 |
| 6806 | | Shift along axis -2.12754240 |
| 6807 | | |
| 6808 | | |
| 6809 | | > select subtract #31 |
| 6810 | | |
| 6811 | | Nothing selected |
| 6812 | | |
| 6813 | | > volume #27 level 1.164 |
| 6814 | | |
| 6815 | | > volume #31 level 0.1055 |
| 6816 | | |
| 6817 | | > hide #!27 models |
| 6818 | | |
| 6819 | | > volume #31 level 0.1343 |
| 6820 | | |
| 6821 | | > color #31 #99BFE5 |
| 6822 | | |
| 6823 | | > rename #31 CS_P4_J267_sharp_flip id #30 |
| 6824 | | |
| 6825 | | > save CS_P4_J267_sharp_flip.mrc #30 |
| 6826 | | |
| 6827 | | > open "/Users/peiyuyu/Documents/Goethe |
| 6828 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 6829 | | > F_B1/cryosparc_P4_J277_008_volume_map_sharp.mrc" |
| 6830 | | |
| 6831 | | Opened cryosparc_P4_J277_008_volume_map_sharp.mrc as #31, grid size |
| 6832 | | 320,320,320, pixel 0.831, shown at level 0.0762, step 2, values float32 |
| 6833 | | |
| 6834 | | > hide #!30 models |
| 6835 | | |
| 6836 | | > select add #31 |
| 6837 | | |
| 6838 | | 2 models selected |
| 6839 | | |
| 6840 | | > ui mousemode right "rotate selected models" |
| 6841 | | |
| 6842 | | > view matrix models |
| 6843 | | > #31,0.50169,-0.77701,0.38022,112.38,-0.8444,-0.53533,0.020189,301.01,0.18786,-0.33119,-0.92467,273.22 |
| 6844 | | |
| 6845 | | > view matrix models |
| 6846 | | > #31,0.77218,-0.62718,0.10187,94.664,-0.63536,-0.764,0.11236,288.83,0.0073633,-0.15149,-0.98843,283.82 |
| 6847 | | |
| 6848 | | > volume flip #31 |
| 6849 | | |
| 6850 | | Opened cryosparc_P4_J277_008_volume_map_sharp.mrc z flip as #32, grid size |
| 6851 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 6852 | | |
| 6853 | | > close #31 |
| 6854 | | |
| 6855 | | > select add #32 |
| 6856 | | |
| 6857 | | 2 models selected |
| 6858 | | |
| 6859 | | > show #!30 models |
| 6860 | | |
| 6861 | | > view matrix models |
| 6862 | | > #32,0.90608,0.27127,-0.3247,15.596,-0.28323,0.95899,0.010829,28.44,0.31432,0.082155,0.94575,-47.16 |
| 6863 | | |
| 6864 | | > view matrix models |
| 6865 | | > #32,0.99239,-0.018676,-0.12173,15.559,0.019459,0.9998,0.0052468,-15.401,0.1216,-0.0075755,0.99255,-16.612 |
| 6866 | | |
| 6867 | | > ui mousemode right "translate selected models" |
| 6868 | | |
| 6869 | | > view matrix models |
| 6870 | | > #32,0.99239,-0.018676,-0.12173,21.14,0.019459,0.9998,0.0052468,-2.6957,0.1216,-0.0075755,0.99255,-9.4459 |
| 6871 | | |
| 6872 | | > fitmap #32 inMap #30 |
| 6873 | | |
| 6874 | | Fit map cryosparc_P4_J277_008_volume_map_sharp.mrc z flip in map |
| 6875 | | CS_P4_J267_sharp_flip.mrc using 327711 points |
| 6876 | | correlation = 0.7196, correlation about mean = 0.3385, overlap = 5671 |
| 6877 | | steps = 204, shift = 3.68, angle = 13.7 degrees |
| 6878 | | |
| 6879 | | Position of cryosparc_P4_J277_008_volume_map_sharp.mrc z flip (#32) relative |
| 6880 | | to CS_P4_J267_sharp_flip.mrc (#30) coordinates: |
| 6881 | | Matrix rotation and translation |
| 6882 | | -0.48144357 0.86501463 -0.14128627 103.86408789 |
| 6883 | | -0.86432037 -0.49531179 -0.08727288 328.52215562 |
| 6884 | | -0.14547307 0.08009963 0.98611442 10.75546195 |
| 6885 | | Axis 0.09633390 0.00240978 -0.99534616 |
| 6886 | | Axis point 148.32654443 133.96556119 0.00000000 |
| 6887 | | Rotation angle (degrees) 119.69088439 |
| 6888 | | Shift along axis 0.09189113 |
| 6889 | | |
| 6890 | | |
| 6891 | | > select subtract #32 |
| 6892 | | |
| 6893 | | Nothing selected |
| 6894 | | |
| 6895 | | > volume #32 level 0.1306 |
| 6896 | | |
| 6897 | | > hide #!30 models |
| 6898 | | |
| 6899 | | > volume #32 level 0.1432 |
| 6900 | | |
| 6901 | | > color #32 #99BFE5 |
| 6902 | | |
| 6903 | | > rename #32 CS_P4_J277_sharp_flip id #31 |
| 6904 | | |
| 6905 | | > save CS_P4_J277_sharp_flip.mrc #31 |
| 6906 | | |
| 6907 | | > open "/Users/peiyuyu/Documents/Goethe |
| 6908 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 6909 | | > F_B1/cryosparc_P4_J285_006_volume_map_sharp.mrc" |
| 6910 | | |
| 6911 | | Opened cryosparc_P4_J285_006_volume_map_sharp.mrc as #32, grid size |
| 6912 | | 320,320,320, pixel 0.831, shown at level 0.0363, step 2, values float32 |
| 6913 | | |
| 6914 | | > hide #!31 models |
| 6915 | | |
| 6916 | | > select add #32 |
| 6917 | | |
| 6918 | | 2 models selected |
| 6919 | | |
| 6920 | | > ui mousemode right "rotate selected models" |
| 6921 | | |
| 6922 | | > view matrix models |
| 6923 | | > #32,0.30706,0.95043,0.049005,-33.318,0.9497,-0.30268,-0.080397,47.411,-0.061579,0.071226,-0.99556,261.24 |
| 6924 | | |
| 6925 | | > volume flip #32 |
| 6926 | | |
| 6927 | | Opened cryosparc_P4_J285_006_volume_map_sharp.mrc z flip as #33, grid size |
| 6928 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 6929 | | |
| 6930 | | > show #!31 models |
| 6931 | | |
| 6932 | | > show #!32 models |
| 6933 | | |
| 6934 | | > view matrix models |
| 6935 | | > #32,0.19586,0.82677,-0.52734,72.951,0.95192,-0.28947,-0.10029,48.053,-0.23556,-0.48235,-0.84371,334.74 |
| 6936 | | |
| 6937 | | > view matrix models |
| 6938 | | > #32,0.038463,0.98737,0.15371,-15.568,0.97818,-0.0057722,-0.20769,22.74,-0.20418,0.15835,-0.96604,265.55 |
| 6939 | | |
| 6940 | | > close #32 |
| 6941 | | |
| 6942 | | > select add #33 |
| 6943 | | |
| 6944 | | 2 models selected |
| 6945 | | |
| 6946 | | > view matrix models |
| 6947 | | > #33,-0.36526,-0.91739,-0.15805,324.89,0.9276,-0.34439,-0.14472,64.223,0.078335,-0.19947,0.97677,17.619 |
| 6948 | | |
| 6949 | | > view matrix models |
| 6950 | | > #33,-0.3296,-0.92737,-0.17708,323.89,0.91639,-0.35937,0.17633,25.386,-0.22716,-0.10416,0.96827,47.503 |
| 6951 | | |
| 6952 | | > select subtract #33 |
| 6953 | | |
| 6954 | | Nothing selected |
| 6955 | | |
| 6956 | | > fitmap #33 inMap #31 |
| 6957 | | |
| 6958 | | Fit map cryosparc_P4_J285_006_volume_map_sharp.mrc z flip in map |
| 6959 | | CS_P4_J277_sharp_flip.mrc using 327344 points |
| 6960 | | correlation = 0.3754, correlation about mean = 0.04431, overlap = 1099 |
| 6961 | | steps = 64, shift = 4.4, angle = 1.24 degrees |
| 6962 | | |
| 6963 | | Position of cryosparc_P4_J285_006_volume_map_sharp.mrc z flip (#33) relative |
| 6964 | | to CS_P4_J277_sharp_flip.mrc (#31) coordinates: |
| 6965 | | Matrix rotation and translation |
| 6966 | | -0.52307807 -0.83656982 -0.16291178 338.77635518 |
| 6967 | | 0.81824450 -0.54640858 0.17864376 66.60900444 |
| 6968 | | -0.23846438 -0.03985703 0.97033300 35.38091339 |
| 6969 | | Axis -0.13076939 0.04521708 0.99038113 |
| 6970 | | Axis point 153.51682890 124.48577590 0.00000000 |
| 6971 | | Rotation angle (degrees) 123.33798629 |
| 6972 | | Shift along axis -6.24912375 |
| 6973 | | |
| 6974 | | |
| 6975 | | > volume #33 level 0.05666 |
| 6976 | | |
| 6977 | | > select add #33 |
| 6978 | | |
| 6979 | | 2 models selected |
| 6980 | | |
| 6981 | | > ui mousemode right "translate selected models" |
| 6982 | | |
| 6983 | | > view matrix models |
| 6984 | | > #33,-0.32295,-0.92807,-0.18543,326.53,0.9135,-0.3569,0.19529,26.322,-0.24742,-0.10632,0.96306,60.406 |
| 6985 | | |
| 6986 | | > fitmap #33 inMap #31 |
| 6987 | | |
| 6988 | | Fit map cryosparc_P4_J285_006_volume_map_sharp.mrc z flip in map |
| 6989 | | CS_P4_J277_sharp_flip.mrc using 194771 points |
| 6990 | | correlation = 0.7316, correlation about mean = 0.2783, overlap = 2429 |
| 6991 | | steps = 104, shift = 3.72, angle = 6.69 degrees |
| 6992 | | |
| 6993 | | Position of cryosparc_P4_J285_006_volume_map_sharp.mrc z flip (#33) relative |
| 6994 | | to CS_P4_J277_sharp_flip.mrc (#31) coordinates: |
| 6995 | | Matrix rotation and translation |
| 6996 | | -0.48329817 -0.86312458 -0.14641999 335.54076959 |
| 6997 | | 0.86340511 -0.49758655 0.08330200 72.08568886 |
| 6998 | | -0.14475663 -0.08616006 0.98570885 33.43360614 |
| 6999 | | Axis -0.09768239 -0.00095880 0.99521718 |
| 7000 | | Axis point 148.43445219 133.71191357 0.00000000 |
| 7001 | | Rotation angle (degrees) 119.84054702 |
| 7002 | | Shift along axis 0.42815717 |
| 7003 | | |
| 7004 | | |
| 7005 | | > select subtract #33 |
| 7006 | | |
| 7007 | | Nothing selected |
| 7008 | | |
| 7009 | | > select add #33 |
| 7010 | | |
| 7011 | | 2 models selected |
| 7012 | | |
| 7013 | | > ui mousemode right "rotate selected models" |
| 7014 | | |
| 7015 | | > view matrix models |
| 7016 | | > #33,-0.6364,0.75107,-0.17577,140.89,-0.74512,-0.6575,-0.11171,345.32,-0.19948,0.059876,0.97807,29.849 |
| 7017 | | |
| 7018 | | > ui mousemode right "translate selected models" |
| 7019 | | |
| 7020 | | > view matrix models |
| 7021 | | > #33,-0.6364,0.75107,-0.17577,136.82,-0.74512,-0.6575,-0.11171,338.86,-0.19948,0.059876,0.97807,29.687 |
| 7022 | | |
| 7023 | | > ui mousemode right "rotate selected models" |
| 7024 | | |
| 7025 | | > view matrix models |
| 7026 | | > #33,-0.73358,0.64738,-0.20678,168.84,-0.63708,-0.76101,-0.12246,339.46,-0.23664,0.041901,0.97069,38.344 |
| 7027 | | |
| 7028 | | > fitmap #33 inMap #31 |
| 7029 | | |
| 7030 | | Fit map cryosparc_P4_J285_006_volume_map_sharp.mrc z flip in map |
| 7031 | | CS_P4_J277_sharp_flip.mrc using 194771 points |
| 7032 | | correlation = 0.4346, correlation about mean = 0.05784, overlap = 1046 |
| 7033 | | steps = 96, shift = 4.05, angle = 4.16 degrees |
| 7034 | | |
| 7035 | | Position of cryosparc_P4_J285_006_volume_map_sharp.mrc z flip (#33) relative |
| 7036 | | to CS_P4_J277_sharp_flip.mrc (#31) coordinates: |
| 7037 | | Matrix rotation and translation |
| 7038 | | -0.58502424 0.80932802 -0.05229508 107.72395607 |
| 7039 | | -0.80221021 -0.58694418 -0.10934029 332.49911712 |
| 7040 | | -0.11918646 -0.02201508 0.99262777 22.68232922 |
| 7041 | | Axis 0.05406170 0.04141143 -0.99767852 |
| 7042 | | Axis point 139.16943260 139.24984383 0.00000000 |
| 7043 | | Rotation angle (degrees) 126.13361686 |
| 7044 | | Shift along axis -3.03667024 |
| 7045 | | |
| 7046 | | |
| 7047 | | > ui mousemode right "translate selected models" |
| 7048 | | |
| 7049 | | > view matrix models |
| 7050 | | > #33,-0.7311,0.66788,-0.13939,159.58,-0.65889,-0.74418,-0.10984,339.55,-0.17709,0.011538,0.98413,33.11 |
| 7051 | | |
| 7052 | | > fitmap #33 inMap #31 |
| 7053 | | |
| 7054 | | Fit map cryosparc_P4_J285_006_volume_map_sharp.mrc z flip in map |
| 7055 | | CS_P4_J277_sharp_flip.mrc using 194771 points |
| 7056 | | correlation = 0.7261, correlation about mean = 0.2429, overlap = 2435 |
| 7057 | | steps = 148, shift = 3.57, angle = 8.91 degrees |
| 7058 | | |
| 7059 | | Position of cryosparc_P4_J285_006_volume_map_sharp.mrc z flip (#33) relative |
| 7060 | | to CS_P4_J277_sharp_flip.mrc (#31) coordinates: |
| 7061 | | Matrix rotation and translation |
| 7062 | | -0.47691102 0.86730315 -0.14262219 103.16958211 |
| 7063 | | -0.86637561 -0.49120809 -0.09004393 328.47939238 |
| 7064 | | -0.14815256 0.08062145 0.98567285 11.48812128 |
| 7065 | | Axis 0.09796714 0.00317460 -0.99518459 |
| 7066 | | Axis point 148.54824739 133.97305983 0.00000000 |
| 7067 | | Rotation angle (degrees) 119.42100612 |
| 7068 | | Shift along axis -0.28278057 |
| 7069 | | |
| 7070 | | |
| 7071 | | > ui mousemode right "rotate selected models" |
| 7072 | | |
| 7073 | | > view matrix models |
| 7074 | | > #33,0.98687,0.16137,0.0076046,-28.356,-0.15803,0.97406,-0.16197,41.243,-0.033544,0.15864,0.98677,-7.5792 |
| 7075 | | |
| 7076 | | > ui mousemode right "translate selected models" |
| 7077 | | |
| 7078 | | > view matrix models |
| 7079 | | > #33,0.98687,0.16137,0.0076046,-22.398,-0.15803,0.97406,-0.16197,51.235,-0.033544,0.15864,0.98677,-4.7399 |
| 7080 | | |
| 7081 | | > fitmap #33 inMap #31 |
| 7082 | | |
| 7083 | | Fit map cryosparc_P4_J285_006_volume_map_sharp.mrc z flip in map |
| 7084 | | CS_P4_J277_sharp_flip.mrc using 194771 points |
| 7085 | | correlation = 0.9896, correlation about mean = 0.9159, overlap = 4214 |
| 7086 | | steps = 120, shift = 4.17, angle = 9.65 degrees |
| 7087 | | |
| 7088 | | Position of cryosparc_P4_J285_006_volume_map_sharp.mrc z flip (#33) relative |
| 7089 | | to CS_P4_J277_sharp_flip.mrc (#31) coordinates: |
| 7090 | | Matrix rotation and translation |
| 7091 | | 0.99997973 -0.00439362 0.00460748 -0.02530815 |
| 7092 | | 0.00439161 0.99999024 0.00044636 -0.51759472 |
| 7093 | | -0.00460939 -0.00042611 0.99998927 1.01725436 |
| 7094 | | Axis -0.06835965 0.72216012 0.68833983 |
| 7095 | | Axis point 171.68283436 0.00000000 1.37920014 |
| 7096 | | Rotation angle (degrees) 0.36563312 |
| 7097 | | Shift along axis 0.32816049 |
| 7098 | | |
| 7099 | | |
| 7100 | | > select subtract #33 |
| 7101 | | |
| 7102 | | Nothing selected |
| 7103 | | |
| 7104 | | > hide #!31 models |
| 7105 | | |
| 7106 | | > show #!31 models |
| 7107 | | |
| 7108 | | > hide #!31 models |
| 7109 | | |
| 7110 | | > volume #33 level 0.06481 |
| 7111 | | |
| 7112 | | > color #33 #99BFE5 |
| 7113 | | |
| 7114 | | > view P1 |
| 7115 | | |
| 7116 | | > rename #33 CS_P4_J285_sharp_flip id #32 |
| 7117 | | |
| 7118 | | > save CS_P4_J285_sharp_flip.mrc #32 |
| 7119 | | |
| 7120 | | > save "/Users/peiyuyu/Documents/Goethe |
| 7121 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 7122 | | > F_B1/20260502_SA-F_B1_workflow.cxs" |
| 7123 | | |
| 7124 | | > save CS_P4_J285_NU_sharp.png supersample 3 transparentBackground true |
| 7125 | | |
| 7126 | | > hide #!32 models |
| 7127 | | |
| 7128 | | > show #!31 models |
| 7129 | | |
| 7130 | | > save CS_P4_J277_NU_sharp.png supersample 3 transparentBackground true |
| 7131 | | |
| 7132 | | > hide #!31 models |
| 7133 | | |
| 7134 | | > show #!30 models |
| 7135 | | |
| 7136 | | > save CS_P4_J267_NU_sharp.png supersample 3 transparentBackground true |
| 7137 | | |
| 7138 | | > open "/Users/peiyuyu/Documents/Goethe |
| 7139 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 7140 | | > F_B1/cryosparc_P4_J282_005_volume_map_sharp.mrc" |
| 7141 | | |
| 7142 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc as #33, grid size |
| 7143 | | 320,320,320, pixel 0.831, shown at level 0.0392, step 2, values float32 |
| 7144 | | |
| 7145 | | > select add #33 |
| 7146 | | |
| 7147 | | 2 models selected |
| 7148 | | |
| 7149 | | > ui mousemode right "rotate selected models" |
| 7150 | | |
| 7151 | | > view matrix models |
| 7152 | | > #33,0.77382,-0.58736,0.23709,68.017,-0.6199,-0.77915,0.092993,287.71,0.13011,-0.21893,-0.96703,293.89 |
| 7153 | | |
| 7154 | | > volume flip sel |
| 7155 | | |
| 7156 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc z flip as #34, grid size |
| 7157 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 7158 | | |
| 7159 | | > select subtract #33 |
| 7160 | | |
| 7161 | | Nothing selected |
| 7162 | | |
| 7163 | | > select add #34 |
| 7164 | | |
| 7165 | | 2 models selected |
| 7166 | | |
| 7167 | | > view matrix models |
| 7168 | | > #34,0.96024,0.18105,-0.2125,4.9844,-0.18441,0.98284,0.0040821,22.146,0.20959,0.035266,0.97715,4.1522 |
| 7169 | | |
| 7170 | | > volume #34 level 0.06153 |
| 7171 | | |
| 7172 | | > ui mousemode right "translate selected models" |
| 7173 | | |
| 7174 | | > view matrix models |
| 7175 | | > #34,0.96024,0.18105,-0.2125,5.3992,-0.18441,0.98284,0.0040821,24.944,0.20959,0.035266,0.97715,-19.851 |
| 7176 | | |
| 7177 | | > fitmap #34 inMap #30 |
| 7178 | | |
| 7179 | | Fit map cryosparc_P4_J282_005_volume_map_sharp.mrc z flip in map |
| 7180 | | CS_P4_J267_sharp_flip.mrc using 179913 points |
| 7181 | | correlation = 0.7668, correlation about mean = 0.2691, overlap = 2384 |
| 7182 | | steps = 132, shift = 4.76, angle = 10.2 degrees |
| 7183 | | |
| 7184 | | Position of cryosparc_P4_J282_005_volume_map_sharp.mrc z flip (#34) relative |
| 7185 | | to CS_P4_J267_sharp_flip.mrc (#30) coordinates: |
| 7186 | | Matrix rotation and translation |
| 7187 | | -0.47859383 0.86460690 -0.15297990 105.65226772 |
| 7188 | | -0.86128787 -0.49615211 -0.10961884 331.90892973 |
| 7189 | | -0.17067851 0.07929683 0.98213077 14.98614385 |
| 7190 | | Axis 0.10880400 0.01019333 -0.99401096 |
| 7191 | | Axis point 150.51207819 135.19673705 0.00000000 |
| 7192 | | Rotation angle (degrees) 119.75601097 |
| 7193 | | Shift along axis -0.01774616 |
| 7194 | | |
| 7195 | | |
| 7196 | | > select subtract #34 |
| 7197 | | |
| 7198 | | Nothing selected |
| 7199 | | |
| 7200 | | > select add #34 |
| 7201 | | |
| 7202 | | 2 models selected |
| 7203 | | |
| 7204 | | > ui mousemode right "rotate selected models" |
| 7205 | | |
| 7206 | | > view matrix models |
| 7207 | | > #34,-0.29858,-0.92833,-0.22151,340.08,0.93935,-0.32688,0.10377,36.917,-0.16874,-0.17709,0.96962,60.484 |
| 7208 | | |
| 7209 | | > fitmap #34 inMap #30 |
| 7210 | | |
| 7211 | | Fit map cryosparc_P4_J282_005_volume_map_sharp.mrc z flip in map |
| 7212 | | CS_P4_J267_sharp_flip.mrc using 179913 points |
| 7213 | | correlation = 0.4184, correlation about mean = 0.03499, overlap = 815.8 |
| 7214 | | steps = 72, shift = 3.19, angle = 2.56 degrees |
| 7215 | | |
| 7216 | | Position of cryosparc_P4_J282_005_volume_map_sharp.mrc z flip (#34) relative |
| 7217 | | to CS_P4_J267_sharp_flip.mrc (#30) coordinates: |
| 7218 | | Matrix rotation and translation |
| 7219 | | 0.99975679 -0.00914856 -0.02006678 -0.29483780 |
| 7220 | | 0.00937915 0.99989072 0.01142689 -12.23865962 |
| 7221 | | 0.01996005 -0.01161232 0.99973334 -0.12571505 |
| 7222 | | Axis -0.46299067 -0.80436995 0.37232865 |
| 7223 | | Axis point 194.44052306 0.00000000 212.15518455 |
| 7224 | | Rotation angle (degrees) 1.42571524 |
| 7225 | | Shift along axis 9.93410983 |
| 7226 | | |
| 7227 | | |
| 7228 | | > ui mousemode right "translate selected models" |
| 7229 | | |
| 7230 | | > view matrix models |
| 7231 | | > #34,-0.29149,-0.93644,-0.19523,323.34,0.9479,-0.31019,0.07257,35.755,-0.12852,-0.16391,0.97807,48.671 |
| 7232 | | |
| 7233 | | > fitmap #34 inMap #30 |
| 7234 | | |
| 7235 | | Fit map cryosparc_P4_J282_005_volume_map_sharp.mrc z flip in map |
| 7236 | | CS_P4_J267_sharp_flip.mrc using 179913 points |
| 7237 | | correlation = 0.9696, correlation about mean = 0.7683, overlap = 3705 |
| 7238 | | steps = 76, shift = 2.39, angle = 2.68 degrees |
| 7239 | | |
| 7240 | | Position of cryosparc_P4_J282_005_volume_map_sharp.mrc z flip (#34) relative |
| 7241 | | to CS_P4_J267_sharp_flip.mrc (#30) coordinates: |
| 7242 | | Matrix rotation and translation |
| 7243 | | 0.99970034 -0.00006024 0.02447927 -4.11138943 |
| 7244 | | 0.00004629 0.99999984 0.00057062 0.05899118 |
| 7245 | | -0.02447930 -0.00056932 0.99970018 3.40337319 |
| 7246 | | Axis -0.02327752 0.99972667 0.00217546 |
| 7247 | | Axis point 136.94050248 0.00000000 169.47617625 |
| 7248 | | Rotation angle (degrees) 1.40308329 |
| 7249 | | Shift along axis 0.16208189 |
| 7250 | | |
| 7251 | | |
| 7252 | | > select subtract #34 |
| 7253 | | |
| 7254 | | Nothing selected |
| 7255 | | |
| 7256 | | > hide #!30 models |
| 7257 | | |
| 7258 | | > show #!32 models |
| 7259 | | |
| 7260 | | > hide #!31 models |
| 7261 | | |
| 7262 | | > volume #34 level 0.06805 |
| 7263 | | |
| 7264 | | > hide #!32 models |
| 7265 | | |
| 7266 | | > view P1 |
| 7267 | | |
| 7268 | | > close #33 |
| 7269 | | |
| 7270 | | > rename #34 CS_P4_J282_sharp_flip id #33 |
| 7271 | | |
| 7272 | | > save CS_P4_J282_sharp_flip.mrc #33 |
| 7273 | | |
| 7274 | | > view P1 |
| 7275 | | |
| 7276 | | > save CS_P4_J282_NU_sharp.png supersample 3 transparentBackground true |
| 7277 | | |
| 7278 | | > save "/Users/peiyuyu/Documents/Goethe |
| 7279 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 7280 | | > F_B1/20260502_SA-F_B1_workflow1.cxs" |
| 7281 | | |
| 7282 | | > color #33 #99BFE5 |
| 7283 | | |
| 7284 | | > save CS_P4_J282_NU_sharp1.png supersample 3 transparentBackground true |
| 7285 | | |
| 7286 | | > save "/Users/peiyuyu/Documents/Goethe |
| 7287 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 7288 | | > F_B1/20260502_SA-F_B1_workflow1.cxs" |
| 7289 | | |
| 7290 | | > open "/Users/peiyuyu/Documents/Goethe |
| 7291 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 7292 | | > F_B1/cryosparc_P4_J287_map_locres.mrc" |
| 7293 | | |
| 7294 | | Opened cryosparc_P4_J287_map_locres.mrc as #34, grid size 320,320,320, pixel |
| 7295 | | 0.831, shown at level 9.77, step 2, values float32 |
| 7296 | | |
| 7297 | | > rename #34 cryosparc_P4_J287_map_locres_forJ282 |
| 7298 | | |
| 7299 | | > select add #34 |
| 7300 | | |
| 7301 | | 2 models selected |
| 7302 | | |
| 7303 | | > view matrix models #34,1,0,0,-1.0935,0,1,0,0.90366,0,0,1,6.3532 |
| 7304 | | |
| 7305 | | > ui mousemode right "rotate selected models" |
| 7306 | | |
| 7307 | | > view matrix models |
| 7308 | | > #34,0.41458,0.88648,0.20563,-71.892,0.90978,-0.4089,-0.07146,80.866,0.020734,0.2167,-0.97602,247.42 |
| 7309 | | |
| 7310 | | > view matrix models |
| 7311 | | > #34,0.40248,0.89866,0.17442,-67.589,0.91533,-0.39227,-0.091056,80.514,-0.013407,0.1963,-0.98045,255.56 |
| 7312 | | |
| 7313 | | > view matrix models |
| 7314 | | > #34,0.3547,0.92587,0.13022,-58.659,0.93218,-0.33944,-0.12576,75.687,-0.072238,0.166,-0.98348,268.27 |
| 7315 | | |
| 7316 | | > fitmap #34 inMap #33 |
| 7317 | | |
| 7318 | | Fit map cryosparc_P4_J287_map_locres_forJ282 in map CS_P4_J282_sharp_flip.mrc |
| 7319 | | using 40893 points |
| 7320 | | correlation = 0.2997, correlation about mean = 0.02148, overlap = 2955 |
| 7321 | | steps = 140, shift = 9.61, angle = 2.61 degrees |
| 7322 | | |
| 7323 | | Position of cryosparc_P4_J287_map_locres_forJ282 (#34) relative to |
| 7324 | | CS_P4_J282_sharp_flip.mrc (#33) coordinates: |
| 7325 | | Matrix rotation and translation |
| 7326 | | 0.77162333 -0.63598985 0.01069393 118.29154644 |
| 7327 | | -0.63440309 -0.76826072 0.08548800 317.77796206 |
| 7328 | | -0.04615378 -0.07274879 -0.99628182 298.10083492 |
| 7329 | | Axis -0.94106809 0.33808548 0.00943679 |
| 7330 | | Axis point 0.00000000 186.80747823 142.52542945 |
| 7331 | | Rotation angle (degrees) 175.17728056 |
| 7332 | | Shift along axis -1.07117046 |
| 7333 | | |
| 7334 | | |
| 7335 | | > volume #34 level 6.762 |
| 7336 | | |
| 7337 | | > view matrix models |
| 7338 | | > #34,0.42433,0.90126,0.087581,-65.663,0.89997,-0.40908,-0.15069,88.731,-0.099987,0.14276,-0.98469,281 |
| 7339 | | |
| 7340 | | > ui mousemode right "translate selected models" |
| 7341 | | |
| 7342 | | > view matrix models |
| 7343 | | > #34,0.42433,0.90126,0.087581,-62.736,0.89997,-0.40908,-0.15069,86.303,-0.099987,0.14276,-0.98469,272.65 |
| 7344 | | |
| 7345 | | > view matrix models |
| 7346 | | > #34,0.42433,0.90126,0.087581,-62.591,0.89997,-0.40908,-0.15069,86.167,-0.099987,0.14276,-0.98469,272.2 |
| 7347 | | |
| 7348 | | > volume #34 level 6.762 |
| 7349 | | |
| 7350 | | > volume #34 level 6.47 |
| 7351 | | |
| 7352 | | > ui tool show "Surface Color" |
| 7353 | | |
| 7354 | | > color sample #33.1 map #34 palette #ff0000:#ffffff:#0000ff outsideColor |
| 7355 | | > #808080 |
| 7356 | | |
| 7357 | | Map values for surface "surface": minimum 0, mean 4.349, maximum 40.76 |
| 7358 | | |
| 7359 | | > select subtract #34 |
| 7360 | | |
| 7361 | | Nothing selected |
| 7362 | | |
| 7363 | | > hide #!34 models |
| 7364 | | |
| 7365 | | > key red-white-blue :0.0 :20.4 :40.8 showTool true |
| 7366 | | |
| 7367 | | > ui mousemode right "color key" |
| 7368 | | |
| 7369 | | > key red-white-blue :0.0 :20.4 :40.8 white: |
| 7370 | | |
| 7371 | | > key red:0.0 white:20.4 blue:40.8 white: white: |
| 7372 | | |
| 7373 | | > key red:0.0 white:20.4 blue:40.8 white: |
| 7374 | | |
| 7375 | | > key red-white-blue :0.0 :20.4 :40.8 |
| 7376 | | |
| 7377 | | > key red-white-blue :0 :2 :4 showTool true |
| 7378 | | |
| 7379 | | > ui mousemode right "color key" |
| 7380 | | |
| 7381 | | > key red-white-blue :0 :2 :4 white: |
| 7382 | | |
| 7383 | | > key red:0 white:2 blue:4 white: white: |
| 7384 | | |
| 7385 | | > color single #33.1 |
| 7386 | | |
| 7387 | | > color sample #33.1 map #34 palette 0,#ff0000:2,#ffffff:4,#0000ff |
| 7388 | | > outsideColor #808080 |
| 7389 | | |
| 7390 | | Map values for surface "surface": minimum 0, mean 4.349, maximum 40.76 |
| 7391 | | |
| 7392 | | > key red-white-blue :0 :2 :4 showTool true |
| 7393 | | |
| 7394 | | > ui mousemode right "color key" |
| 7395 | | |
| 7396 | | > key red-white-blue :0 :2 :4 showTool true |
| 7397 | | |
| 7398 | | > key red-white-blue :0 :2 :4 white: |
| 7399 | | |
| 7400 | | > key red:0 white:2 blue:4 white: white: |
| 7401 | | |
| 7402 | | > key red:0 white:2 blue:4 white: white:0 |
| 7403 | | |
| 7404 | | > key red:0 white:2 blue:4 white:1 white:0 |
| 7405 | | |
| 7406 | | > key red:0 white:2 blue:2 white:1 white:0 |
| 7407 | | |
| 7408 | | > key red:0 white:3 blue:2 white:1 white:0 |
| 7409 | | |
| 7410 | | > key red:4 white:3 blue:2 white:1 white:0 |
| 7411 | | |
| 7412 | | > key gnbu :4 :3 :2 :1 :0 |
| 7413 | | |
| 7414 | | > key esmfold :4 :3 :2 :1 :0 |
| 7415 | | |
| 7416 | | > key dark2-5 :4 :3 :2 :1 :0 |
| 7417 | | |
| 7418 | | > key pastel1-5 :4 :3 :2 :1 :0 |
| 7419 | | |
| 7420 | | > key blues :4 :3 :2 :1 :0 |
| 7421 | | |
| 7422 | | > key accent-5 :4 :3 :2 :1 :0 |
| 7423 | | |
| 7424 | | > color sample #33.1 map #34 palette 0,#ff0000:2,#ffffff:4,#0000ff |
| 7425 | | > outsideColor #808080 |
| 7426 | | |
| 7427 | | Map values for surface "surface": minimum 0, mean 4.349, maximum 40.76 |
| 7428 | | |
| 7429 | | > color sample #33.1 map #34 palette 0,#ff0000:2,#ffffff:4,#0000ff |
| 7430 | | > outsideColor #808080 |
| 7431 | | |
| 7432 | | Map values for surface "surface": minimum 0, mean 4.349, maximum 40.76 |
| 7433 | | |
| 7434 | | > color sample #33.1 map #34 palette 0,#0000ff:2,#7f7fff:4,#ffffff |
| 7435 | | > outsideColor #808080 |
| 7436 | | |
| 7437 | | Map values for surface "surface": minimum 0, mean 4.349, maximum 40.76 |
| 7438 | | |
| 7439 | | > key blue:0 #7f7fff:2 white:4 showTool true |
| 7440 | | |
| 7441 | | > ui mousemode right "color key" |
| 7442 | | |
| 7443 | | > key blue:0 #7f7fff:2 white:4 showTool true |
| 7444 | | |
| 7445 | | > key blue:0 #7f7fff:2 white:4 white: |
| 7446 | | |
| 7447 | | > key blue:0 #7f7fff:2 white:4 white: white: |
| 7448 | | |
| 7449 | | > color sample #33.1 map #34 palette |
| 7450 | | > 0,#0000ff:1,#7f7fff:2,#ffffff:3,#ff7f7f:4,#ff0000 outsideColor #808080 |
| 7451 | | |
| 7452 | | Map values for surface "surface": minimum 0, mean 4.349, maximum 40.76 |
| 7453 | | |
| 7454 | | > key blue:0 #7f7fff:1 white:2 #ff7f7f:3 red:4 showTool true |
| 7455 | | |
| 7456 | | > ui mousemode right "color key" |
| 7457 | | |
| 7458 | | > color sample #33.1 map #34 palette |
| 7459 | | > 0,#0000ff:1,#7f7fff:2,#ffffff:3,#ff7f7f:4,#ff0000 outsideColor #808080 |
| 7460 | | |
| 7461 | | Map values for surface "surface": minimum 0, mean 4.349, maximum 40.76 |
| 7462 | | |
| 7463 | | > color sample #33.1 map #34 palette |
| 7464 | | > 0,#ff0000:1,#ff7f7f:2,#ffffff:3,#7f7fff:4,#0000ff outsideColor #808080 |
| 7465 | | |
| 7466 | | Map values for surface "surface": minimum 0, mean 4.349, maximum 40.76 |
| 7467 | | |
| 7468 | | > color sample #33.1 map #34 palette |
| 7469 | | > 0,#ff0000:2,#ff7f7f:4,#ffffff:5,#7f7fff:8,#0000ff outsideColor #808080 |
| 7470 | | |
| 7471 | | Map values for surface "surface": minimum 0, mean 4.349, maximum 40.76 |
| 7472 | | |
| 7473 | | > volume #33 level 0.08574 |
| 7474 | | |
| 7475 | | > key red:0 #ff7f7f:2 white:4 #7f7fff:5 blue:8 showTool true |
| 7476 | | |
| 7477 | | > key red:0 #ff7f7f:2 white:4 #7f7fff:6 blue:8 showTool true |
| 7478 | | |
| 7479 | | > color sample #33.1 map #34 palette |
| 7480 | | > 0,#0000ff:2,#7f7fff:4,#ffffff:6,#ff7f7f:8,#ff0000 outsideColor #808080 |
| 7481 | | |
| 7482 | | Map values for surface "surface": minimum 0, mean 4.269, maximum 35.43 |
| 7483 | | |
| 7484 | | > color sample #33.1 map #34 palette |
| 7485 | | > 0,#0000ff:2,#7f7fff:4,#ffffff:6,#ff7f7f:8,#fff6c0 outsideColor #808080 |
| 7486 | | |
| 7487 | | Map values for surface "surface": minimum 0, mean 4.269, maximum 35.43 |
| 7488 | | |
| 7489 | | > key blue:0 #7f7fff:2 white:4 #ff7f7f:6 #fff6c0:8 showTool true |
| 7490 | | |
| 7491 | | > color sample #33.1 map #34 palette |
| 7492 | | > 0,#0000ff:2,#7f7fff:4,#ffffff:6,#ffad7f:8,#fff6c0 outsideColor #808080 |
| 7493 | | |
| 7494 | | Map values for surface "surface": minimum 0, mean 4.269, maximum 35.43 |
| 7495 | | |
| 7496 | | > color sample #33.1 map #34 palette |
| 7497 | | > 0,#0000ff:2,#7f7fff:4,#ffffff:6,#ff967f:8,#fff6c0 outsideColor #808080 |
| 7498 | | |
| 7499 | | Map values for surface "surface": minimum 0, mean 4.269, maximum 35.43 |
| 7500 | | |
| 7501 | | > key blue:0 #7f7fff:2 white:4 #ff967f:6 #fff6c0:8 showTool true |
| 7502 | | |
| 7503 | | > color sample #33.1 map #34 palette |
| 7504 | | > 0,#fff6c0:2,#ff967f:4,#ffffff:6,#7f7fff:8,#0000ff outsideColor #808080 |
| 7505 | | |
| 7506 | | Map values for surface "surface": minimum 0, mean 4.269, maximum 35.43 |
| 7507 | | |
| 7508 | | > color sample #33.1 map #34 palette |
| 7509 | | > 0,#0000ff:2,#7f7fff:4,#ffffff:6,#ff967f:8,#fff6c0 outsideColor #808080 |
| 7510 | | |
| 7511 | | Map values for surface "surface": minimum 0, mean 4.269, maximum 35.43 |
| 7512 | | |
| 7513 | | > key blue:0 #7f7fff:2 white:4 #ff967f:6 #fff6c0:8 showTool true |
| 7514 | | |
| 7515 | | > color sample #33.1 map #34 palette |
| 7516 | | > 0,#00a7ff:2,#7f7fff:4,#ffffff:6,#ff967f:8,#fff6c0 outsideColor #808080 |
| 7517 | | |
| 7518 | | Map values for surface "surface": minimum 0, mean 4.269, maximum 35.43 |
| 7519 | | |
| 7520 | | > key #00a7ff:0 #7f7fff:2 white:4 #ff967f:6 #fff6c0:8 showTool true |
| 7521 | | |
| 7522 | | > color sample #33.1 map #34 palette |
| 7523 | | > 0,#00a7ff:2,#d2a5ff:4,#ffffff:6,#ff967f:8,#fff6c0 outsideColor #808080 |
| 7524 | | |
| 7525 | | Map values for surface "surface": minimum 0, mean 4.269, maximum 35.43 |
| 7526 | | |
| 7527 | | > key #00a7ff:0 #d2a5ff:2 white:4 #ff967f:6 #fff6c0:8 showTool true |
| 7528 | | |
| 7529 | | > save "/Users/peiyuyu/Documents/Goethe |
| 7530 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 7531 | | > F_B1/20260502_SA-F_B1_workflow2.cxs" |
| 7532 | | |
| 7533 | | > open "/Users/peiyuyu/Documents/Goethe |
| 7534 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 7535 | | > F_B1/cryosparc_P4_J288_map_locres.mrc" |
| 7536 | | |
| 7537 | | Opened cryosparc_P4_J288_map_locres.mrc as #36, grid size 320,320,320, pixel |
| 7538 | | 0.831, shown at level 8.93, step 2, values float32 |
| 7539 | | |
| 7540 | | > hide #35 models |
| 7541 | | |
| 7542 | | > show #35 models |
| 7543 | | |
| 7544 | | > hide #!36 models |
| 7545 | | |
| 7546 | | > hide #!33 models |
| 7547 | | |
| 7548 | | > show #!33 models |
| 7549 | | |
| 7550 | | > rename #36 cryosparc_P4_J288_map_locres_forJ285 |
| 7551 | | |
| 7552 | | > hide #!33 models |
| 7553 | | |
| 7554 | | > show #!32 models |
| 7555 | | |
| 7556 | | > hide #35 models |
| 7557 | | |
| 7558 | | > show #!36 models |
| 7559 | | |
| 7560 | | > select add #36 |
| 7561 | | |
| 7562 | | 2 models selected |
| 7563 | | |
| 7564 | | > ui mousemode right "rotate selected models" |
| 7565 | | |
| 7566 | | > view matrix models |
| 7567 | | > #36,0.54984,-0.77769,0.30476,129.28,-0.81459,-0.57994,-0.010221,331.17,0.18469,-0.24264,-0.95237,263.23 |
| 7568 | | |
| 7569 | | > ui mousemode right "translate selected models" |
| 7570 | | |
| 7571 | | > view matrix models |
| 7572 | | > #36,0.54984,-0.77769,0.30476,119.97,-0.81459,-0.57994,-0.010221,318.58,0.18469,-0.24264,-0.95237,280.96 |
| 7573 | | |
| 7574 | | > volume #36 level 6.234 |
| 7575 | | |
| 7576 | | > select subtract #36 |
| 7577 | | |
| 7578 | | Nothing selected |
| 7579 | | |
| 7580 | | > ui tool show "Surface Color" |
| 7581 | | |
| 7582 | | > color sample #32.1 map #36 palette |
| 7583 | | > 0,#00a7ff:2,#d2a5ff:4,#ffffff:6,#ff967f:8,#fff6c0 outsideColor #808080 |
| 7584 | | |
| 7585 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 7586 | | |
| 7587 | | > hide #!36 models |
| 7588 | | |
| 7589 | | > show #!33 models |
| 7590 | | |
| 7591 | | > hide #!33 models |
| 7592 | | |
| 7593 | | > show #!33 models |
| 7594 | | |
| 7595 | | > hide #!32 models |
| 7596 | | |
| 7597 | | > show #!32 models |
| 7598 | | |
| 7599 | | > hide #!33 models |
| 7600 | | |
| 7601 | | > key #00a7ff:0 #d2a5ff:2 white:4 #ff967f:6 #fff6c0:8 showTool true |
| 7602 | | |
| 7603 | | > show #!36 models |
| 7604 | | |
| 7605 | | > hide #!36 models |
| 7606 | | |
| 7607 | | > key #00a7ff:0 #d2a5ff:2 white:4 #ff967f:6 #fff6c0:8 white: |
| 7608 | | |
| 7609 | | > key #00a7ff:0 #d2a5ff:2 white:4 #ff967f:6 #fff6c0:8 |
| 7610 | | |
| 7611 | | Fetching compressed palette custom from |
| 7612 | | https://www.colourlovers.com/api/palettes?keywords=custom&format=json&numResults=100 |
| 7613 | | Could not find palette custom at COLOURlovers.com using keyword search |
| 7614 | | |
| 7615 | | > color sample #32.1 map #36 palette |
| 7616 | | > 0,#00a7ff:2,#d2a5ff:4,#ffffff:6,#ff967f:8,#fff6c0 outsideColor #808080 |
| 7617 | | |
| 7618 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 7619 | | |
| 7620 | | > key #00a7ff:0 #d2a5ff:2 white:4 #ff967f:6 #fff6c0:8 showTool true |
| 7621 | | |
| 7622 | | > hide #!32 models |
| 7623 | | |
| 7624 | | > show #35 models |
| 7625 | | |
| 7626 | | > show #!32 models |
| 7627 | | |
| 7628 | | > select add #35 |
| 7629 | | |
| 7630 | | 1 model selected |
| 7631 | | |
| 7632 | | > view matrix models #35,1,0,0,-1.208,0,1,0,-1.3654,0,0,1,1.1486 |
| 7633 | | |
| 7634 | | > select subtract #35 |
| 7635 | | |
| 7636 | | Nothing selected |
| 7637 | | |
| 7638 | | > select add #35 |
| 7639 | | |
| 7640 | | 1 model selected |
| 7641 | | |
| 7642 | | > select subtract #35 |
| 7643 | | |
| 7644 | | Nothing selected |
| 7645 | | |
| 7646 | | > key #00a7ff:0 #d2a5ff:2 white:4 #ff967f:6 #fff6c0:8 showTool true |
| 7647 | | |
| 7648 | | > ui mousemode right "color key" |
| 7649 | | |
| 7650 | | > select add #35 |
| 7651 | | |
| 7652 | | 1 model selected |
| 7653 | | |
| 7654 | | > show #35 |
| 7655 | | |
| 7656 | | > view W35 |
| 7657 | | |
| 7658 | | Expected an objects specifier or a view name or a keyword |
| 7659 | | |
| 7660 | | > view #35 |
| 7661 | | |
| 7662 | | No displayed objects specified. |
| 7663 | | |
| 7664 | | > close #35 |
| 7665 | | |
| 7666 | | > key #00a7ff:0 #d2a5ff:2 white:4 #ff967f:6 #fff6c0:8 showTool true |
| 7667 | | |
| 7668 | | > ui mousemode right "color key" |
| 7669 | | |
| 7670 | | > hide #!32 models |
| 7671 | | |
| 7672 | | > show #!33 models |
| 7673 | | |
| 7674 | | > hide #!33 models |
| 7675 | | |
| 7676 | | > show #!33 models |
| 7677 | | |
| 7678 | | > hide #!33 models |
| 7679 | | |
| 7680 | | > show #!33 models |
| 7681 | | |
| 7682 | | > volume #33 level 0.06991 |
| 7683 | | |
| 7684 | | > hide #!33 models |
| 7685 | | |
| 7686 | | > show #!32 models |
| 7687 | | |
| 7688 | | > save "/Users/peiyuyu/Documents/Goethe |
| 7689 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 7690 | | > F_B1/20260502_SA-F_B1_workflow2.cxs" |
| 7691 | | |
| 7692 | | > color sample #32.1 map #36 palette |
| 7693 | | > 0,#00a7ff:2,#99bfe5:4,#ffffff:6,#ff967f:8,#fff6c0 outsideColor #808080 |
| 7694 | | |
| 7695 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 7696 | | |
| 7697 | | > key #00a7ff:0 #99bfe5:2 white:4 #fff6c0:6 #ff967f:8 showTool true |
| 7698 | | |
| 7699 | | > ui mousemode right "color key" |
| 7700 | | |
| 7701 | | > color sample #32.1 map #36 palette |
| 7702 | | > 0,#00a7ff:2,#99bfe5:4,#ffffff:6,#fff6c0:8,#ff967f outsideColor #808080 |
| 7703 | | |
| 7704 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 7705 | | |
| 7706 | | > color sample #32.1 map #36 palette |
| 7707 | | > 0,#d2a5ff:2,#99bfe5:4,#ffffff:6,#fff6c0:8,#ff967f outsideColor #808080 |
| 7708 | | |
| 7709 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 7710 | | |
| 7711 | | > key #d2a5ff:0 #99bfe5:2 white:4 #fff6c0:6 #ff967f:8 showTool true |
| 7712 | | |
| 7713 | | > view #35 |
| 7714 | | |
| 7715 | | No objects specified. |
| 7716 | | |
| 7717 | | > color sample #32.1 map #36 palette |
| 7718 | | > 0,#e7c4ff:2,#99bfe5:4,#ffffff:6,#fff6c0:8,#ff967f outsideColor #808080 |
| 7719 | | |
| 7720 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 7721 | | |
| 7722 | | > key #e7c4ff:0 #99bfe5:2 white:4 #fff6c0:6 #ff967f:8 showTool true |
| 7723 | | |
| 7724 | | > save "/Users/peiyuyu/Documents/Goethe |
| 7725 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 7726 | | > F_B1/20260502_SA-F_B1_workflow2.cxs" |
| 7727 | | |
| 7728 | | > color sample #32.1 map #36 palette |
| 7729 | | > 0,#e7c4ff:2,#99bfe5:4,#5da0ff:6,#fff6c0:8,#ff967f outsideColor #808080 |
| 7730 | | |
| 7731 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 7732 | | |
| 7733 | | > color sample #32.1 map #36 palette |
| 7734 | | > 0,#e7c4ff:2,#e0bfe8:4,#99bfe5:6,#fff6c0:8,#ff967f outsideColor #808080 |
| 7735 | | |
| 7736 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 7737 | | |
| 7738 | | > color sample #32.1 map #36 palette |
| 7739 | | > 0,#e7c4ff:2,#e0dee8:4,#99bfe5:6,#fff6c0:8,#ff967f outsideColor #808080 |
| 7740 | | |
| 7741 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 7742 | | |
| 7743 | | > color sample #32.1 map #36 palette |
| 7744 | | > 0,#e7c4ff:2,#e0dee8:4,#99bfe5:6,#fff6c0:8,#ff967f outsideColor #808080 |
| 7745 | | |
| 7746 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 7747 | | |
| 7748 | | > color sample #32.1 map #36 palette |
| 7749 | | > 0,#e7c4ff:2,#6676ed:4,#99bfe5:6,#fff6c0:8,#ff967f outsideColor #808080 |
| 7750 | | |
| 7751 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 7752 | | |
| 7753 | | > color sample #32.1 map #36 palette |
| 7754 | | > 0,#e7c4ff:2,#6676ed:4,#99bfe5:6,#fff6c0:8,#f4fff3 outsideColor #808080 |
| 7755 | | |
| 7756 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 7757 | | |
| 7758 | | > color sample #32.1 map #36 palette |
| 7759 | | > 0,#e7c4ff:2,#99bfe5:4,#99bfe5:6,#fff6c0:8,#f4fff3 outsideColor #808080 |
| 7760 | | |
| 7761 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 7762 | | |
| 7763 | | > color sample #32.1 map #36 palette |
| 7764 | | > 0,#e7c4ff:2,#99bfe5:4,#6676ed:6,#fff6c0:8,#f4fff3 outsideColor #808080 |
| 7765 | | |
| 7766 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 7767 | | |
| 7768 | | > color sample #32.1 map #36 palette |
| 7769 | | > 0,#e7c4ff:2,#99bfe5:4,#99bfe5:6,#fff6c0:8,#f4fff3 outsideColor #808080 |
| 7770 | | |
| 7771 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 7772 | | |
| 7773 | | > color sample #32.1 map #36 palette |
| 7774 | | > 0,#e7c4ff:2,#799bea:4,#99bfe5:6,#fff6c0:8,#f4fff3 outsideColor #808080 |
| 7775 | | |
| 7776 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 7777 | | |
| 7778 | | > color sample #32.1 map #36 palette |
| 7779 | | > 0,#e7c4ff:2,#5756ea:4,#99bfe5:6,#fff6c0:8,#f4fff3 outsideColor #808080 |
| 7780 | | |
| 7781 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 7782 | | |
| 7783 | | > color sample #32.1 map #36 palette |
| 7784 | | > 0,#e7c4ff:2,#527dea:4,#99bfe5:6,#fff6c0:8,#f4fff3 outsideColor #808080 |
| 7785 | | |
| 7786 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 7787 | | |
| 7788 | | > color sample #32.1 map #36 palette |
| 7789 | | > 0,#ffdedd:2,#527dea:4,#99bfe5:6,#fff6c0:8,#f4fff3 outsideColor #808080 |
| 7790 | | |
| 7791 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 7792 | | |
| 7793 | | > color sample #32.1 map #36 palette |
| 7794 | | > 0,#ffc9a3:2,#527dea:4,#99bfe5:6,#fff6c0:8,#f4fff3 outsideColor #808080 |
| 7795 | | |
| 7796 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 7797 | | |
| 7798 | | > color sample #32.1 map #36 palette |
| 7799 | | > 0,#ffb17f:2,#527dea:4,#99bfe5:6,#fff6c0:8,#f4fff3 outsideColor #808080 |
| 7800 | | |
| 7801 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 7802 | | |
| 7803 | | > key #ffb17f:0 #527dea:2 #99bfe5:4 #fff6c0:6 #f4fff3:8 showTool true |
| 7804 | | |
| 7805 | | > ui mousemode right "color key" |
| 7806 | | |
| 7807 | | > save "/Users/peiyuyu/Documents/Goethe |
| 7808 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 7809 | | > F_B1/20260502_SA-F_B1_workflow3.cxs" |
| 7810 | | |
| 7811 | | > hide #!32 models |
| 7812 | | |
| 7813 | | > show #!33 models |
| 7814 | | |
| 7815 | | > hide #!33 models |
| 7816 | | |
| 7817 | | > show #!32 models |
| 7818 | | |
| 7819 | | > hide #!32 models |
| 7820 | | |
| 7821 | | > show #!33 models |
| 7822 | | |
| 7823 | | > hide #!33 models |
| 7824 | | |
| 7825 | | > show #!32 models |
| 7826 | | |
| 7827 | | > volume #34 step 1 |
| 7828 | | |
| 7829 | | > volume #36 step 1 |
| 7830 | | |
| 7831 | | > show #!36 models |
| 7832 | | |
| 7833 | | > volume flip #36 |
| 7834 | | |
| 7835 | | Opened cryosparc_P4_J288_map_locres_forJ285 z flip as #38, grid size |
| 7836 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 7837 | | |
| 7838 | | > hide #37 models |
| 7839 | | |
| 7840 | | > ui mousemode right "rotate selected models" |
| 7841 | | |
| 7842 | | > hide #!32 models |
| 7843 | | |
| 7844 | | > show #!32 models |
| 7845 | | |
| 7846 | | > hide #!38 models |
| 7847 | | |
| 7848 | | > show #!38 models |
| 7849 | | |
| 7850 | | > select add #38 |
| 7851 | | |
| 7852 | | 2 models selected |
| 7853 | | |
| 7854 | | > view matrix models |
| 7855 | | > #38,-0.22965,0.94651,-0.22667,61.099,-0.95753,-0.26145,-0.12161,309.34,-0.17437,0.18912,0.96635,13.289 |
| 7856 | | |
| 7857 | | > view matrix models |
| 7858 | | > #38,-0.47581,-0.84028,-0.25987,345.82,0.86453,-0.50117,0.037622,69.811,-0.16185,-0.20677,0.96491,66.334 |
| 7859 | | |
| 7860 | | > view matrix models |
| 7861 | | > #38,-0.72836,0.6263,-0.27792,180.88,-0.55109,-0.77649,-0.30556,349.01,-0.40718,-0.069403,0.91071,88.496 |
| 7862 | | |
| 7863 | | > view matrix models |
| 7864 | | > #38,-0.422,0.88275,-0.20657,93.707,-0.7937,-0.46985,-0.38638,351.04,-0.43814,0.0009021,0.89891,84.657 |
| 7865 | | |
| 7866 | | > fitmap #38 inMap #32 |
| 7867 | | |
| 7868 | | Fit map cryosparc_P4_J288_map_locres_forJ285 z flip in map |
| 7869 | | CS_P4_J285_sharp_flip.mrc using 1112104 points |
| 7870 | | correlation = 0.2781, correlation about mean = -0.02765, overlap = 7.665e+04 |
| 7871 | | steps = 172, shift = 16.7, angle = 11.9 degrees |
| 7872 | | |
| 7873 | | Position of cryosparc_P4_J288_map_locres_forJ285 z flip (#38) relative to |
| 7874 | | CS_P4_J285_sharp_flip.mrc (#32) coordinates: |
| 7875 | | Matrix rotation and translation |
| 7876 | | -0.15731459 0.96448973 -0.21215945 50.76791567 |
| 7877 | | -0.83839054 -0.24396591 -0.48742375 323.17714111 |
| 7878 | | -0.52187488 0.10119361 0.84699849 86.68679965 |
| 7879 | | Axis 0.30630695 0.16117090 -0.93818974 |
| 7880 | | Axis point 169.99659509 146.99779714 0.00000000 |
| 7881 | | Rotation angle (degrees) 106.08964492 |
| 7882 | | Shift along axis -13.69134858 |
| 7883 | | |
| 7884 | | |
| 7885 | | > ui mousemode right "translate selected models" |
| 7886 | | |
| 7887 | | > view matrix models |
| 7888 | | > #38,-0.29391,0.88572,-0.35933,106.9,-0.78003,-0.43953,-0.4454,363.34,-0.55243,0.14938,0.82006,93.87 |
| 7889 | | |
| 7890 | | > ui mousemode right "rotate selected models" |
| 7891 | | |
| 7892 | | > volume #38 level 4.082 |
| 7893 | | |
| 7894 | | > view matrix models |
| 7895 | | > #38,-0.44764,0.81063,-0.37749,140.89,-0.70715,-0.57929,-0.40543,367.19,-0.54732,0.085454,0.83255,100.3 |
| 7896 | | |
| 7897 | | > ui mousemode right "translate selected models" |
| 7898 | | |
| 7899 | | [Repeated 1 time(s)] |
| 7900 | | |
| 7901 | | > view matrix models |
| 7902 | | > #38,-0.44764,0.81063,-0.37749,135.23,-0.70715,-0.57929,-0.40543,367.93,-0.54732,0.085454,0.83255,97.332 |
| 7903 | | |
| 7904 | | > view matrix models |
| 7905 | | > #38,-0.44764,0.81063,-0.37749,134.86,-0.70715,-0.57929,-0.40543,366.07,-0.54732,0.085454,0.83255,96.949 |
| 7906 | | |
| 7907 | | > select subtract #38 |
| 7908 | | |
| 7909 | | Nothing selected |
| 7910 | | |
| 7911 | | > hide #!38 models |
| 7912 | | |
| 7913 | | > color sample #32.1 map #38 palette |
| 7914 | | > 0,#ffb17f:2,#527dea:4,#99bfe5:6,#fff6c0:8,#f4fff3 outsideColor #808080 |
| 7915 | | |
| 7916 | | Map values for surface "surface": minimum 0, mean 4.286, maximum 42.51 |
| 7917 | | |
| 7918 | | > open "/Users/peiyuyu/Documents/Goethe |
| 7919 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 7920 | | > F_B1/CS_P4_J282_sharp_flip.mrc" |
| 7921 | | |
| 7922 | | Opened CS_P4_J282_sharp_flip.mrc as #39, grid size 320,320,320, pixel 0.831, |
| 7923 | | shown at level 0.0392, step 2, values float32 |
| 7924 | | |
| 7925 | | > hide #!32 models |
| 7926 | | |
| 7927 | | > close #39 |
| 7928 | | |
| 7929 | | > open "/Users/peiyuyu/Documents/Goethe |
| 7930 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 7931 | | > F_B1/cryosparc_P4_J282_005_volume_map_sharp.mrc" |
| 7932 | | |
| 7933 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc as #35, grid size |
| 7934 | | 320,320,320, pixel 0.831, shown at level 0.0392, step 2, values float32 |
| 7935 | | |
| 7936 | | > open "/Users/peiyuyu/Documents/Goethe |
| 7937 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 7938 | | > F_B1/cryosparc_P4_J287_map_locres.mrc" |
| 7939 | | |
| 7940 | | Opened cryosparc_P4_J287_map_locres_forJ282 as #39, grid size 320,320,320, |
| 7941 | | pixel 0.831, shown at level 9.77, step 2, values float32 |
| 7942 | | |
| 7943 | | > volume #39 level 4.139 |
| 7944 | | |
| 7945 | | > open "/Users/peiyuyu/Documents/Goethe |
| 7946 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 7947 | | > F_B1/cryosparc_P4_J287_map_locres.mrc" |
| 7948 | | |
| 7949 | | Opened cryosparc_P4_J287_map_locres_forJ282 as #40, grid size 320,320,320, |
| 7950 | | pixel 0.831, shown at level 9.77, step 2, values float32 |
| 7951 | | |
| 7952 | | > close #40 |
| 7953 | | |
| 7954 | | > color sample #35.1 map #39 palette |
| 7955 | | > 0,#ffb17f:2,#527dea:4,#99bfe5:6,#fff6c0:8,#f4fff3 outsideColor #808080 |
| 7956 | | |
| 7957 | | Map values for surface "surface": minimum 0, mean 4.417, maximum 39.82 |
| 7958 | | |
| 7959 | | > hide #!39 models |
| 7960 | | |
| 7961 | | > volume #35 step 1 |
| 7962 | | |
| 7963 | | > volume #35 level 0.06749 |
| 7964 | | |
| 7965 | | > color sample #35.1 map #39 palette |
| 7966 | | > 0,#ffb17f:1,#527dea:2,#99bfe5:3,#fff6c0:4,#f4fff3 outsideColor #808080 |
| 7967 | | |
| 7968 | | Map values for surface "surface": minimum 3.044, mean 3.567, maximum 40.68 |
| 7969 | | |
| 7970 | | > color sample #35.1 map #39 palette |
| 7971 | | > 0,#ffb17f:2,#527dea:3,#99bfe5:4,#fff6c0:5,#f4fff3 outsideColor #808080 |
| 7972 | | |
| 7973 | | Map values for surface "surface": minimum 3.044, mean 3.567, maximum 40.68 |
| 7974 | | |
| 7975 | | > color sample #35.1 map #39 palette |
| 7976 | | > 2,#ffb17f:3,#527dea:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 7977 | | |
| 7978 | | Map values for surface "surface": minimum 3.044, mean 3.567, maximum 40.68 |
| 7979 | | |
| 7980 | | > close #32 |
| 7981 | | |
| 7982 | | > close #33 |
| 7983 | | |
| 7984 | | > save "/Users/peiyuyu/Documents/Goethe |
| 7985 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 7986 | | > F_B1/20260503_SA-F_B1_workflow.cxs" |
| 7987 | | |
| 7988 | | > close #39 |
| 7989 | | |
| 7990 | | > close #38 |
| 7991 | | |
| 7992 | | > close #36 |
| 7993 | | |
| 7994 | | > close #35 |
| 7995 | | |
| 7996 | | > show #!34 models |
| 7997 | | |
| 7998 | | > hide #!34 models |
| 7999 | | |
| 8000 | | > close #34 |
| 8001 | | |
| 8002 | | > open "/Users/peiyuyu/Documents/Goethe |
| 8003 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 8004 | | > F_B1/cryosparc_P4_J282_005_volume_map_sharp.mrc" |
| 8005 | | |
| 8006 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc as #32, grid size |
| 8007 | | 320,320,320, pixel 0.831, shown at level 0.0392, step 2, values float32 |
| 8008 | | |
| 8009 | | > open "/Users/peiyuyu/Documents/Goethe |
| 8010 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 8011 | | > F_B1/cryosparc_P4_J285_006_volume_map_sharp.mrc" |
| 8012 | | |
| 8013 | | Opened cryosparc_P4_J285_006_volume_map_sharp.mrc as #33, grid size |
| 8014 | | 320,320,320, pixel 0.831, shown at level 0.0363, step 2, values float32 |
| 8015 | | |
| 8016 | | > open "/Users/peiyuyu/Documents/Goethe |
| 8017 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 8018 | | > F_B1/cryosparc_P4_J287_map_locres.mrc" |
| 8019 | | |
| 8020 | | Opened cryosparc_P4_J287_map_locres.mrc as #34, grid size 320,320,320, pixel |
| 8021 | | 0.831, shown at level 9.77, step 2, values float32 |
| 8022 | | |
| 8023 | | > open "/Users/peiyuyu/Documents/Goethe |
| 8024 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 8025 | | > F_B1/cryosparc_P4_J288_map_locres.mrc" |
| 8026 | | |
| 8027 | | Opened cryosparc_P4_J288_map_locres.mrc as #35, grid size 320,320,320, pixel |
| 8028 | | 0.831, shown at level 8.93, step 2, values float32 |
| 8029 | | |
| 8030 | | > rename #37 Color key id #36 |
| 8031 | | |
| 8032 | | Expected a keyword |
| 8033 | | |
| 8034 | | > rename #37 Color_key id #36 |
| 8035 | | |
| 8036 | | Cannot change id of 2D overlay models (Color_key #37) |
| 8037 | | |
| 8038 | | > hide #!33 models |
| 8039 | | |
| 8040 | | > hide #!35 models |
| 8041 | | |
| 8042 | | > volume #34 level 6.298 |
| 8043 | | |
| 8044 | | > volume #34 level 3.171 |
| 8045 | | |
| 8046 | | > volume #34 level 3.779 |
| 8047 | | |
| 8048 | | > hide #!34 models |
| 8049 | | |
| 8050 | | > ui tool show "Surface Color" |
| 8051 | | |
| 8052 | | No surface chosen for coloring |
| 8053 | | |
| 8054 | | > show #!34 models |
| 8055 | | |
| 8056 | | > hide #!34 models |
| 8057 | | |
| 8058 | | > color sample #32.1 map #34 palette |
| 8059 | | > 2,#ffb17f:3,#527dea:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 8060 | | |
| 8061 | | Map values for surface "surface": minimum 0, mean 4.417, maximum 39.82 |
| 8062 | | |
| 8063 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 8064 | | |
| 8065 | | > ui mousemode right "color key" |
| 8066 | | |
| 8067 | | Could not find palette custom at COLOURlovers.com using keyword search |
| 8068 | | |
| 8069 | | > show #37 models |
| 8070 | | |
| 8071 | | > key pos 0.558738,0.385722 size 0.0255738,0.0868715 |
| 8072 | | |
| 8073 | | > key pos 0.723062,0.0751149 |
| 8074 | | |
| 8075 | | > key pos 0.862303,0.0795068 |
| 8076 | | |
| 8077 | | > key pos 0.846838,0.0779833 |
| 8078 | | |
| 8079 | | > select add #37 |
| 8080 | | |
| 8081 | | 1 model selected |
| 8082 | | |
| 8083 | | > key pos 0.819995,0.0796645 |
| 8084 | | |
| 8085 | | > key pos 0.812107,0.0767711 |
| 8086 | | |
| 8087 | | > key pos 0.825223,0.13099 |
| 8088 | | |
| 8089 | | > select subtract #37 |
| 8090 | | |
| 8091 | | Nothing selected |
| 8092 | | |
| 8093 | | > undo |
| 8094 | | |
| 8095 | | [Repeated 3 time(s)] |
| 8096 | | |
| 8097 | | > color sample #32.1 map #34 palette |
| 8098 | | > 2,#ffb17f:3,#527dea:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 8099 | | |
| 8100 | | Map values for surface "surface": minimum 0, mean 4.417, maximum 39.82 |
| 8101 | | |
| 8102 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 8103 | | |
| 8104 | | > show #37 models |
| 8105 | | |
| 8106 | | > select add #37 |
| 8107 | | |
| 8108 | | 1 model selected |
| 8109 | | |
| 8110 | | > view matrix models |
| 8111 | | > #37,0.70959,0.62592,0.32358,0.61324,-0.29635,0.68175,-0.66888,-0.0021786,-0.63926,0.37874,0.66925,0.69431 |
| 8112 | | |
| 8113 | | > close #37 |
| 8114 | | |
| 8115 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 8116 | | |
| 8117 | | > ui mousemode right "color key" |
| 8118 | | |
| 8119 | | > hide #!32 models |
| 8120 | | |
| 8121 | | > show #!32 models |
| 8122 | | |
| 8123 | | > volume #32 level 0.04587 |
| 8124 | | |
| 8125 | | > volume #32 level 0.04884 |
| 8126 | | |
| 8127 | | > key pos 0.437353,0.539212 size 0.000454748,0.0481037 |
| 8128 | | |
| 8129 | | > hide #36 models |
| 8130 | | |
| 8131 | | > show #36 models |
| 8132 | | |
| 8133 | | > close #36 |
| 8134 | | |
| 8135 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 8136 | | |
| 8137 | | > ui mousemode right "color key" |
| 8138 | | |
| 8139 | | > ui mousemode right translate |
| 8140 | | |
| 8141 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 8142 | | |
| 8143 | | [Repeated 1 time(s)] |
| 8144 | | |
| 8145 | | > ui mousemode right "color key" |
| 8146 | | |
| 8147 | | > ui mousemode right translate |
| 8148 | | |
| 8149 | | > ui mousemode middle 'color key' |
| 8150 | | |
| 8151 | | > key border false |
| 8152 | | |
| 8153 | | > key border true |
| 8154 | | |
| 8155 | | > key borderWidth 1.0 |
| 8156 | | |
| 8157 | | > key borderWidth 0.0 |
| 8158 | | |
| 8159 | | > key borderWidth 1.0 |
| 8160 | | |
| 8161 | | > key colorTreatment distinct |
| 8162 | | |
| 8163 | | > key colorTreatment blended |
| 8164 | | |
| 8165 | | > ui mousemode middle translate |
| 8166 | | |
| 8167 | | > ui mousemode middle "color key" |
| 8168 | | |
| 8169 | | > key borderColor #bfbfbf |
| 8170 | | |
| 8171 | | > key borderColor default |
| 8172 | | |
| 8173 | | > key borderColor #bfbfbf |
| 8174 | | |
| 8175 | | > key borderColor #030303 |
| 8176 | | |
| 8177 | | > key borderColor #020202 |
| 8178 | | |
| 8179 | | > key borderColor #010202 |
| 8180 | | |
| 8181 | | > key borderColor black |
| 8182 | | |
| 8183 | | > key labelColor #bfbfbf |
| 8184 | | |
| 8185 | | > key labelColor default |
| 8186 | | |
| 8187 | | > key fontSize 23 |
| 8188 | | |
| 8189 | | > key fontSize 22 |
| 8190 | | |
| 8191 | | > key fontSize 21 |
| 8192 | | |
| 8193 | | > key fontSize 20 |
| 8194 | | |
| 8195 | | > key fontSize 21 |
| 8196 | | |
| 8197 | | > key fontSize 22 |
| 8198 | | |
| 8199 | | > key fontSize 23 |
| 8200 | | |
| 8201 | | > key fontSize 24 |
| 8202 | | |
| 8203 | | > key bold true |
| 8204 | | |
| 8205 | | > key italic true |
| 8206 | | |
| 8207 | | > key italic false |
| 8208 | | |
| 8209 | | > key numericLabelSpacing equal |
| 8210 | | |
| 8211 | | > key numericLabelSpacing proportional |
| 8212 | | |
| 8213 | | > key labelSide left/top |
| 8214 | | |
| 8215 | | > key labelSide right/bottom |
| 8216 | | |
| 8217 | | > key justification left |
| 8218 | | |
| 8219 | | > key labelSide right/bottom |
| 8220 | | |
| 8221 | | > key justification decimal |
| 8222 | | |
| 8223 | | > key justification right |
| 8224 | | |
| 8225 | | > key justification left |
| 8226 | | |
| 8227 | | > key labelOffset 1.0 |
| 8228 | | |
| 8229 | | > key labelOffset 2.0 |
| 8230 | | |
| 8231 | | > key labelOffset 3.0 |
| 8232 | | |
| 8233 | | > key labelOffset 4.0 |
| 8234 | | |
| 8235 | | > key labelOffset 5.0 |
| 8236 | | |
| 8237 | | > key labelOffset 4.0 |
| 8238 | | |
| 8239 | | > key labelOffset 3.0 |
| 8240 | | |
| 8241 | | > key labelOffset 2.0 |
| 8242 | | |
| 8243 | | > key ticks true |
| 8244 | | |
| 8245 | | > key ticks false |
| 8246 | | |
| 8247 | | > key size 0.26000,0.05000 |
| 8248 | | |
| 8249 | | > key size 0.27000,0.05000 |
| 8250 | | |
| 8251 | | > key size 0.26000,0.05000 |
| 8252 | | |
| 8253 | | > key size 0.25000,0.05000 |
| 8254 | | |
| 8255 | | > key size 0.24000,0.05000 |
| 8256 | | |
| 8257 | | > key size 0.23000,0.05000 |
| 8258 | | |
| 8259 | | > key size 0.22000,0.05000 |
| 8260 | | |
| 8261 | | > key size 0.22000,0.04000 |
| 8262 | | |
| 8263 | | > key size 0.21000,0.04000 |
| 8264 | | |
| 8265 | | > key size 0.20000,0.04000 |
| 8266 | | |
| 8267 | | > key size 0.19000,0.04000 |
| 8268 | | |
| 8269 | | > key bold false |
| 8270 | | |
| 8271 | | > key borderWidth 2.0 |
| 8272 | | |
| 8273 | | > key border false |
| 8274 | | |
| 8275 | | > key border true |
| 8276 | | |
| 8277 | | > key size 0.18000,0.04000 |
| 8278 | | |
| 8279 | | > key size 0.17000,0.04000 |
| 8280 | | |
| 8281 | | > key size 0.17000,0.03000 |
| 8282 | | |
| 8283 | | > key size 0.17000,0.02000 |
| 8284 | | |
| 8285 | | > key size 0.16000,0.02000 |
| 8286 | | |
| 8287 | | > key size 0.15000,0.02000 |
| 8288 | | |
| 8289 | | > key size 0.14000,0.02000 |
| 8290 | | |
| 8291 | | > key size 0.15000,0.02000 |
| 8292 | | |
| 8293 | | > key size 0.14000,0.02000 |
| 8294 | | |
| 8295 | | > key size 0.14000,0.01000 |
| 8296 | | |
| 8297 | | > key size 0.14000,0.02000 |
| 8298 | | |
| 8299 | | > key size 0.14000,0.01000 |
| 8300 | | |
| 8301 | | > key size 0.14000,0.02000 |
| 8302 | | |
| 8303 | | > key size 0.14000,0.03000 |
| 8304 | | |
| 8305 | | > key size 0.14000,0.04000 |
| 8306 | | |
| 8307 | | > key size 0.14000,0.03000 |
| 8308 | | |
| 8309 | | > key bold true |
| 8310 | | |
| 8311 | | > save CS_P4_J282_locres_sharp.png supersample 3 transparentBackground true |
| 8312 | | |
| 8313 | | > hide #!32 models |
| 8314 | | |
| 8315 | | > show #!33 models |
| 8316 | | |
| 8317 | | > show #!35 models |
| 8318 | | |
| 8319 | | > volume #33 level 0.0299 |
| 8320 | | |
| 8321 | | > undo |
| 8322 | | |
| 8323 | | > show #!35 models |
| 8324 | | |
| 8325 | | > volume #35 level 3.774 |
| 8326 | | |
| 8327 | | > hide #!35 models |
| 8328 | | |
| 8329 | | > color sample #33.1 map #35 palette |
| 8330 | | > 2,#ffb17f:3,#527dea:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 8331 | | |
| 8332 | | Map values for surface "surface": minimum 0, mean 4.383, maximum 41.2 |
| 8333 | | |
| 8334 | | > volume #33 level 0.03991 |
| 8335 | | |
| 8336 | | > volume #33 level 0.049 |
| 8337 | | |
| 8338 | | > volume #33 level 0.06811 |
| 8339 | | |
| 8340 | | > volume #33 level 0.05628 |
| 8341 | | |
| 8342 | | > volume #33 level 0.05719 |
| 8343 | | |
| 8344 | | > ui mousemode right translate |
| 8345 | | |
| 8346 | | [Repeated 1 time(s)] |
| 8347 | | |
| 8348 | | > select add #37 |
| 8349 | | |
| 8350 | | 1 model selected |
| 8351 | | |
| 8352 | | > ui mousemode right translate |
| 8353 | | |
| 8354 | | > select subtract #37 |
| 8355 | | |
| 8356 | | Nothing selected |
| 8357 | | |
| 8358 | | > view #33 |
| 8359 | | |
| 8360 | | > volume #33 level 0.05264 |
| 8361 | | |
| 8362 | | > volume #33 level 0.05082 |
| 8363 | | |
| 8364 | | > save CS_P4_J285_NU_sharp.png supersample 3 transparentBackground true |
| 8365 | | |
| 8366 | | > save CS_P4_J285_locres_sharp.png supersample 3 transparentBackground true |
| 8367 | | |
| 8368 | | > hide #!33 models |
| 8369 | | |
| 8370 | | > show #!32 models |
| 8371 | | |
| 8372 | | > volume #32 level 0.05478 |
| 8373 | | |
| 8374 | | > save CS_P4_J282_locres_sharp.png supersample 3 transparentBackground true |
| 8375 | | |
| 8376 | | > save "/Users/peiyuyu/Documents/Goethe |
| 8377 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 8378 | | > F_B1/20260503_SA-F_B1_workflow1.cxs" |
| 8379 | | |
| 8380 | | > show #!33 models |
| 8381 | | |
| 8382 | | > hide #!33 models |
| 8383 | | |
| 8384 | | > volume flip #32 |
| 8385 | | |
| 8386 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc z flip as #38, grid size |
| 8387 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 8388 | | |
| 8389 | | > color sample #38.1 map #34 palette |
| 8390 | | > 2,#ffb17f:3,#527dea:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 8391 | | |
| 8392 | | Map values for surface "surface": minimum 0, mean 4.643, maximum 46.37 |
| 8393 | | |
| 8394 | | > select add #38 |
| 8395 | | |
| 8396 | | 2 models selected |
| 8397 | | |
| 8398 | | > select subtract #38 |
| 8399 | | |
| 8400 | | Nothing selected |
| 8401 | | |
| 8402 | | > show #!34 models |
| 8403 | | |
| 8404 | | > hide #!34 models |
| 8405 | | |
| 8406 | | > show #!32 models |
| 8407 | | |
| 8408 | | > select add #38 |
| 8409 | | |
| 8410 | | 2 models selected |
| 8411 | | |
| 8412 | | > ui mousemode right "rotate selected models" |
| 8413 | | |
| 8414 | | > view matrix models |
| 8415 | | > #38,0.96218,0.27239,0.0026401,-33.277,0.27218,-0.96095,-0.049909,243.62,-0.011058,0.04874,-0.99875,256.5 |
| 8416 | | |
| 8417 | | > fitmap #38 inMap #32 |
| 8418 | | |
| 8419 | | Fit map cryosparc_P4_J282_005_volume_map_sharp.mrc z flip in map |
| 8420 | | cryosparc_P4_J282_005_volume_map_sharp.mrc using 208382 points |
| 8421 | | correlation = 0.3998, correlation about mean = 0.04433, overlap = 423.6 |
| 8422 | | steps = 132, shift = 4.37, angle = 6.4 degrees |
| 8423 | | |
| 8424 | | Position of cryosparc_P4_J282_005_volume_map_sharp.mrc z flip (#38) relative |
| 8425 | | to cryosparc_P4_J282_005_volume_map_sharp.mrc (#32) coordinates: |
| 8426 | | Matrix rotation and translation |
| 8427 | | 0.98647244 0.16384860 0.00507628 -21.42824077 |
| 8428 | | 0.16385811 -0.98468453 -0.05955579 259.77996198 |
| 8429 | | -0.00475960 0.05958193 -0.99821207 257.16545694 |
| 8430 | | Axis 0.99660933 0.08227896 0.00007951 |
| 8431 | | Axis point 0.00000000 126.91521003 132.48208787 |
| 8432 | | Rotation angle (degrees) 176.57330142 |
| 8433 | | Shift along axis 0.03928788 |
| 8434 | | |
| 8435 | | |
| 8436 | | > select subtract #38 |
| 8437 | | |
| 8438 | | Nothing selected |
| 8439 | | |
| 8440 | | > select add #38 |
| 8441 | | |
| 8442 | | 2 models selected |
| 8443 | | |
| 8444 | | > view matrix models |
| 8445 | | > #38,-0.55814,-0.81354,-0.1632,351.46,-0.79796,0.58019,-0.16319,187.27,0.22745,0.039147,-0.973,224.57 |
| 8446 | | |
| 8447 | | > ui mousemode right "translate selected models" |
| 8448 | | |
| 8449 | | > view matrix models |
| 8450 | | > #38,-0.55814,-0.81354,-0.1632,337.55,-0.79796,0.58019,-0.16319,184.57,0.22745,0.039147,-0.973,224.6 |
| 8451 | | |
| 8452 | | > fitmap #38 inMap #32 |
| 8453 | | |
| 8454 | | Fit map cryosparc_P4_J282_005_volume_map_sharp.mrc z flip in map |
| 8455 | | cryosparc_P4_J282_005_volume_map_sharp.mrc using 208382 points |
| 8456 | | correlation = 0.4208, correlation about mean = 0.04628, overlap = 461.9 |
| 8457 | | steps = 76, shift = 2.33, angle = 4.27 degrees |
| 8458 | | |
| 8459 | | Position of cryosparc_P4_J282_005_volume_map_sharp.mrc z flip (#38) relative |
| 8460 | | to cryosparc_P4_J282_005_volume_map_sharp.mrc (#32) coordinates: |
| 8461 | | Matrix rotation and translation |
| 8462 | | -0.49732082 -0.85447621 -0.15014131 334.86076124 |
| 8463 | | -0.83743504 0.51801846 -0.17423960 201.03033487 |
| 8464 | | 0.22665956 0.03908062 -0.97318968 225.34606158 |
| 8465 | | Axis 0.49228130 -0.86954734 0.03932608 |
| 8466 | | Axis point 210.12674218 0.00000000 138.36301119 |
| 8467 | | Rotation angle (degrees) 167.48677512 |
| 8468 | | Shift along axis -1.09772430 |
| 8469 | | |
| 8470 | | |
| 8471 | | > select subtract #38 |
| 8472 | | |
| 8473 | | Nothing selected |
| 8474 | | |
| 8475 | | > select add #38 |
| 8476 | | |
| 8477 | | 2 models selected |
| 8478 | | |
| 8479 | | > view matrix models |
| 8480 | | > #38,-0.49732,-0.85448,-0.15014,335.05,-0.83744,0.51802,-0.17424,201.05,0.22666,0.039081,-0.97319,225.03 |
| 8481 | | |
| 8482 | | > ui mousemode right "rotate selected models" |
| 8483 | | |
| 8484 | | > view matrix models |
| 8485 | | > #38,-0.016367,0.99633,0.084023,-21.562,0.99914,0.013095,0.039355,-10.502,0.03811,0.084595,-0.99569,247.71 |
| 8486 | | |
| 8487 | | > ui mousemode right "translate selected models" |
| 8488 | | |
| 8489 | | > view matrix models |
| 8490 | | > #38,-0.016367,0.99633,0.084023,-11.414,0.99914,0.013095,0.039355,-8.4507,0.03811,0.084595,-0.99569,248.91 |
| 8491 | | |
| 8492 | | > fitmap #38 inMap #32 |
| 8493 | | |
| 8494 | | Fit map cryosparc_P4_J282_005_volume_map_sharp.mrc z flip in map |
| 8495 | | cryosparc_P4_J282_005_volume_map_sharp.mrc using 208382 points |
| 8496 | | correlation = 0.4638, correlation about mean = 0.04825, overlap = 549.2 |
| 8497 | | steps = 212, shift = 3.48, angle = 14 degrees |
| 8498 | | |
| 8499 | | Position of cryosparc_P4_J282_005_volume_map_sharp.mrc z flip (#38) relative |
| 8500 | | to cryosparc_P4_J282_005_volume_map_sharp.mrc (#32) coordinates: |
| 8501 | | Matrix rotation and translation |
| 8502 | | -0.11733678 0.98529275 -0.12421864 36.78201449 |
| 8503 | | 0.98526620 0.13117004 0.10974943 -32.51895072 |
| 8504 | | 0.12442908 -0.10951079 -0.98616672 260.89985464 |
| 8505 | | Axis -0.66139292 -0.75003960 -0.00008010 |
| 8506 | | Axis point 18.21387939 0.00000000 132.49954907 |
| 8507 | | Rotation angle (degrees) 170.45882216 |
| 8508 | | Shift along axis 0.04223943 |
| 8509 | | |
| 8510 | | |
| 8511 | | > select subtract #38 |
| 8512 | | |
| 8513 | | Nothing selected |
| 8514 | | |
| 8515 | | > hide #!32 models |
| 8516 | | |
| 8517 | | > color sample #38.1 map #34 palette |
| 8518 | | > 2,#ffb17f:3,#527dea:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 8519 | | |
| 8520 | | Map values for surface "surface": minimum 3.043, mean 3.992, maximum 41.6 |
| 8521 | | |
| 8522 | | > show #!34 models |
| 8523 | | |
| 8524 | | > volume flip #34 |
| 8525 | | |
| 8526 | | Opened cryosparc_P4_J287_map_locres.mrc z flip as #39, grid size 320,320,320, |
| 8527 | | pixel 0.831, shown at step 1, values float32 |
| 8528 | | |
| 8529 | | > select add #39 |
| 8530 | | |
| 8531 | | 2 models selected |
| 8532 | | |
| 8533 | | > ui mousemode right "rotate selected models" |
| 8534 | | |
| 8535 | | > view matrix models |
| 8536 | | > #39,0.92903,0.36038,-0.083822,-29.473,0.36808,-0.92323,0.1103,201.34,-0.037639,-0.13332,-0.99036,275.09 |
| 8537 | | |
| 8538 | | > show #!34 models |
| 8539 | | |
| 8540 | | > fitmap #39 imapt #34 |
| 8541 | | |
| 8542 | | Expected a keyword |
| 8543 | | |
| 8544 | | > fitmap #39 inMap #34 |
| 8545 | | |
| 8546 | | Fit map cryosparc_P4_J287_map_locres.mrc z flip in map |
| 8547 | | cryosparc_P4_J287_map_locres.mrc using 2056381 points |
| 8548 | | correlation = 0.8371, correlation about mean = 0.04941, overlap = 1.057e+08 |
| 8549 | | steps = 612, shift = 15.2, angle = 38.8 degrees |
| 8550 | | |
| 8551 | | Position of cryosparc_P4_J287_map_locres.mrc z flip (#39) relative to |
| 8552 | | cryosparc_P4_J287_map_locres.mrc (#34) coordinates: |
| 8553 | | Matrix rotation and translation |
| 8554 | | 0.49975558 0.86379414 -0.06406286 -38.69544656 |
| 8555 | | 0.86380943 -0.49157793 0.11038297 66.86486459 |
| 8556 | | 0.06385627 -0.11050260 -0.99182234 270.06743869 |
| 8557 | | Axis -0.86536171 -0.50114779 0.00005993 |
| 8558 | | Axis point 0.00000000 54.63554700 132.55703766 |
| 8559 | | Rotation angle (degrees) 172.66756182 |
| 8560 | | Shift along axis -0.00743630 |
| 8561 | | |
| 8562 | | |
| 8563 | | > select subtract #39 |
| 8564 | | |
| 8565 | | Nothing selected |
| 8566 | | |
| 8567 | | > hide #!39 models |
| 8568 | | |
| 8569 | | > show #!39 models |
| 8570 | | |
| 8571 | | > select add #39 |
| 8572 | | |
| 8573 | | 2 models selected |
| 8574 | | |
| 8575 | | > hide #37 models |
| 8576 | | |
| 8577 | | > hide #!38 models |
| 8578 | | |
| 8579 | | > view matrix models |
| 8580 | | > #39,0.37237,-0.92808,0.0029165,218.26,-0.92659,-0.37195,-0.055474,318.12,0.052569,0.017955,-0.99846,254.69 |
| 8581 | | |
| 8582 | | > ui mousemode right "translate selected models" |
| 8583 | | |
| 8584 | | > view matrix models |
| 8585 | | > #39,0.37237,-0.92808,0.0029165,208.8,-0.92659,-0.37195,-0.055474,313.9,0.052569,0.017955,-0.99846,255.36 |
| 8586 | | |
| 8587 | | > ui mousemode right "rotate selected models" |
| 8588 | | |
| 8589 | | > view matrix models |
| 8590 | | > #39,0.66947,-0.74091,0.05349,135.56,-0.73453,-0.67101,-0.10103,334.54,0.11074,0.028346,-0.99344,245.27 |
| 8591 | | |
| 8592 | | > ui mousemode right "translate selected models" |
| 8593 | | |
| 8594 | | > view matrix models |
| 8595 | | > #39,0.66947,-0.74091,0.05349,211.58,-0.73453,-0.67101,-0.10103,368.3,0.11074,0.028346,-0.99344,245.76 |
| 8596 | | |
| 8597 | | > view matrix models |
| 8598 | | > #39,0.66947,-0.74091,0.05349,241.5,-0.73453,-0.67101,-0.10103,381.69,0.11074,0.028346,-0.99344,243.08 |
| 8599 | | |
| 8600 | | > view matrix models |
| 8601 | | > #39,0.66947,-0.74091,0.05349,287.43,-0.73453,-0.67101,-0.10103,402.31,0.11074,0.028346,-0.99344,237.25 |
| 8602 | | |
| 8603 | | > ui mousemode right "rotate selected models" |
| 8604 | | |
| 8605 | | > view matrix models |
| 8606 | | > #39,-0.41494,0.89077,-0.18535,241.42,0.8738,0.44692,0.19168,-11.043,0.25358,-0.082424,-0.9638,229.13 |
| 8607 | | |
| 8608 | | > view matrix models |
| 8609 | | > #39,0.99948,-0.019992,0.025148,145.79,-0.021975,-0.99645,0.081215,326.06,0.023435,-0.081726,-0.99638,264.88 |
| 8610 | | |
| 8611 | | > ui mousemode right "translate selected models" |
| 8612 | | |
| 8613 | | > view matrix models |
| 8614 | | > #39,0.99948,-0.019992,0.025148,-4.0949,-0.021975,-0.99645,0.081215,259.26,0.023435,-0.081726,-0.99638,270.42 |
| 8615 | | |
| 8616 | | > select subtract #39 |
| 8617 | | |
| 8618 | | Nothing selected |
| 8619 | | |
| 8620 | | > hide #!34 models |
| 8621 | | |
| 8622 | | > color sample #38.1 map #34 palette |
| 8623 | | > 2,#ffb17f:3,#527dea:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 8624 | | |
| 8625 | | Map values for surface "surface": minimum 3.043, mean 3.992, maximum 41.6 |
| 8626 | | |
| 8627 | | > hide #!39 models |
| 8628 | | |
| 8629 | | > show #!38 models |
| 8630 | | |
| 8631 | | > open "/Users/peiyuyu/Documents/Goethe |
| 8632 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 8633 | | > F_B1/cryosparc_P4_J282_005_volume_map_sharp.mrc" |
| 8634 | | |
| 8635 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc as #40, grid size |
| 8636 | | 320,320,320, pixel 0.831, shown at level 0.0392, step 2, values float32 |
| 8637 | | |
| 8638 | | > hide #!38 models |
| 8639 | | |
| 8640 | | > open "/Users/peiyuyu/Documents/Goethe |
| 8641 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 8642 | | > F_B1/cryosparc_P4_J287_map_locres.mrc" |
| 8643 | | |
| 8644 | | Opened cryosparc_P4_J287_map_locres.mrc as #41, grid size 320,320,320, pixel |
| 8645 | | 0.831, shown at level 9.77, step 2, values float32 |
| 8646 | | |
| 8647 | | > volume flip #40 |
| 8648 | | |
| 8649 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc z flip as #42, grid size |
| 8650 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 8651 | | |
| 8652 | | > volume flip #41 |
| 8653 | | |
| 8654 | | Opened cryosparc_P4_J287_map_locres.mrc z flip as #43, grid size 320,320,320, |
| 8655 | | pixel 0.831, shown at step 1, values float32 |
| 8656 | | |
| 8657 | | > volume #43 level 4.042 |
| 8658 | | |
| 8659 | | > color sample #42.1 map #43 palette |
| 8660 | | > 2,#ffb17f:3,#527dea:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 8661 | | |
| 8662 | | Map values for surface "surface": minimum 0, mean 4.484, maximum 42.57 |
| 8663 | | |
| 8664 | | > hide #!43 models |
| 8665 | | |
| 8666 | | > hide #!42 models |
| 8667 | | |
| 8668 | | > show #!38 models |
| 8669 | | |
| 8670 | | > show #!39 models |
| 8671 | | |
| 8672 | | > show #!40 models |
| 8673 | | |
| 8674 | | > show #!41 models |
| 8675 | | |
| 8676 | | > show #!42 models |
| 8677 | | |
| 8678 | | > show #!43 models |
| 8679 | | |
| 8680 | | > close #43 |
| 8681 | | |
| 8682 | | > close #38 |
| 8683 | | |
| 8684 | | > close #42 |
| 8685 | | |
| 8686 | | > close #41 |
| 8687 | | |
| 8688 | | > close #40 |
| 8689 | | |
| 8690 | | > close #39 |
| 8691 | | |
| 8692 | | > close #37 |
| 8693 | | |
| 8694 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 8695 | | |
| 8696 | | > ui mousemode right "color key" |
| 8697 | | |
| 8698 | | > hide #36 models |
| 8699 | | |
| 8700 | | > volume flip #32 |
| 8701 | | |
| 8702 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc z flip as #37, grid size |
| 8703 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 8704 | | |
| 8705 | | > volume flip #34 |
| 8706 | | |
| 8707 | | Opened cryosparc_P4_J287_map_locres.mrc z flip as #38, grid size 320,320,320, |
| 8708 | | pixel 0.831, shown at step 1, values float32 |
| 8709 | | |
| 8710 | | > volume flip #33 |
| 8711 | | |
| 8712 | | Opened cryosparc_P4_J285_006_volume_map_sharp.mrc z flip as #39, grid size |
| 8713 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 8714 | | |
| 8715 | | > volume flip #35 |
| 8716 | | |
| 8717 | | Opened cryosparc_P4_J288_map_locres.mrc z flip as #40, grid size 320,320,320, |
| 8718 | | pixel 0.831, shown at step 1, values float32 |
| 8719 | | |
| 8720 | | > hide #!39 models |
| 8721 | | |
| 8722 | | > hide #!40 models |
| 8723 | | |
| 8724 | | > hide #!38 models |
| 8725 | | |
| 8726 | | > color sample #37.1 map #38 palette |
| 8727 | | > 2,#ffb17f:3,#527dea:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 8728 | | |
| 8729 | | Map values for surface "surface": minimum 0, mean 3.731, maximum 41.43 |
| 8730 | | |
| 8731 | | > hide #!37 models |
| 8732 | | |
| 8733 | | > show #!37 models |
| 8734 | | |
| 8735 | | > hide #!37 models |
| 8736 | | |
| 8737 | | > show #!39 models |
| 8738 | | |
| 8739 | | > color sample #39.1 map #40 palette |
| 8740 | | > 2,#ffb17f:3,#527dea:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 8741 | | |
| 8742 | | Map values for surface "surface": minimum 0, mean 3.67, maximum 40.73 |
| 8743 | | |
| 8744 | | > volume #39 level 0.05082 |
| 8745 | | |
| 8746 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 8747 | | |
| 8748 | | > show #36 models |
| 8749 | | |
| 8750 | | > select add #36 |
| 8751 | | |
| 8752 | | 1 model selected |
| 8753 | | |
| 8754 | | > key pos 0.366468,0.16715 |
| 8755 | | |
| 8756 | | > key pos 0.360149,0.168599 |
| 8757 | | |
| 8758 | | > key labelColor #bfbfbf |
| 8759 | | |
| 8760 | | > key labelColor default |
| 8761 | | |
| 8762 | | > key labelColor #bfbfbf |
| 8763 | | |
| 8764 | | > key labelColor white |
| 8765 | | |
| 8766 | | > key labelColor default |
| 8767 | | |
| 8768 | | > key ticks true |
| 8769 | | |
| 8770 | | > key tickLength 8.0 |
| 8771 | | |
| 8772 | | > key tickThickness 3.0 |
| 8773 | | |
| 8774 | | > key tickThickness 2.0 |
| 8775 | | |
| 8776 | | > key tickThickness 1.0 |
| 8777 | | |
| 8778 | | > key tickLength 10.0 |
| 8779 | | |
| 8780 | | > key tickLength 8.0 |
| 8781 | | |
| 8782 | | > key tickThickness 2.0 |
| 8783 | | |
| 8784 | | > key tickThickness 1.0 |
| 8785 | | |
| 8786 | | > key tickThickness 0.0 |
| 8787 | | |
| 8788 | | > key tickThickness 1.0 |
| 8789 | | |
| 8790 | | > key tickThickness 2.0 |
| 8791 | | |
| 8792 | | > key ticks false |
| 8793 | | |
| 8794 | | > key labelOffset -1.0 |
| 8795 | | |
| 8796 | | > key labelOffset -2.0 |
| 8797 | | |
| 8798 | | > key labelOffset -1.0 |
| 8799 | | |
| 8800 | | > key labelOffset 0.0 |
| 8801 | | |
| 8802 | | > key labelOffset 1.0 |
| 8803 | | |
| 8804 | | > key labelOffset 2.0 |
| 8805 | | |
| 8806 | | > key labelOffset 3.0 |
| 8807 | | |
| 8808 | | > key labelOffset 4.0 |
| 8809 | | |
| 8810 | | > view matrix models #36,1,0,0,-0.86321,0,1,0,-0.41592,0,0,1,0.9887 |
| 8811 | | |
| 8812 | | > select subtract #36 |
| 8813 | | |
| 8814 | | Nothing selected |
| 8815 | | |
| 8816 | | > select add #36 |
| 8817 | | |
| 8818 | | 1 model selected |
| 8819 | | |
| 8820 | | > view matrix models #36,1,0,0,-99.598,0,1,0,-41.849,0,0,1,63.569 |
| 8821 | | |
| 8822 | | > view matrix models #36,1,0,0,-99.715,0,1,0,-41.895,0,0,1,63.621 |
| 8823 | | |
| 8824 | | > view matrix models #36,1,0,0,-102.61,0,1,0,-42.915,0,0,1,63.831 |
| 8825 | | |
| 8826 | | > view matrix models #36,1,0,0,-126.49,0,1,0,-56.195,0,0,1,105.79 |
| 8827 | | |
| 8828 | | > view matrix models #36,1,0,0,-152.42,0,1,0,-56.013,0,0,1,31.21 |
| 8829 | | |
| 8830 | | > view matrix models #36,1,0,0,-131.29,0,1,0,-47.985,0,0,1,24.738 |
| 8831 | | |
| 8832 | | > view matrix models #36,1,0,0,-121.36,0,1,0,-44.649,0,0,1,25.261 |
| 8833 | | |
| 8834 | | > view matrix models #36,1,0,0,-121.37,0,1,0,-44.69,0,0,1,25.567 |
| 8835 | | |
| 8836 | | > view matrix models #36,1,0,0,-123.18,0,1,0,-45.336,0,0,1,25.776 |
| 8837 | | |
| 8838 | | > close #36 |
| 8839 | | |
| 8840 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 8841 | | |
| 8842 | | > ui mousemode right "color key" |
| 8843 | | |
| 8844 | | > key pos 0.377773,0.195303 |
| 8845 | | |
| 8846 | | > key pos 0.377554,0.185959 |
| 8847 | | |
| 8848 | | > key borderWidth 3.0 |
| 8849 | | |
| 8850 | | > key labelOffset 1.0 |
| 8851 | | |
| 8852 | | > key labelOffset 2.0 |
| 8853 | | |
| 8854 | | > key labelOffset 3.0 |
| 8855 | | |
| 8856 | | > key labelOffset 4.0 |
| 8857 | | |
| 8858 | | > key labelOffset 5.0 |
| 8859 | | |
| 8860 | | > key labelOffset 6.0 |
| 8861 | | |
| 8862 | | > key size 0.24000,0.05000 |
| 8863 | | |
| 8864 | | > key size 0.23000,0.05000 |
| 8865 | | |
| 8866 | | > key size 0.23000,0.04000 |
| 8867 | | |
| 8868 | | > key size 0.23000,0.03000 |
| 8869 | | |
| 8870 | | > key size 0.22000,0.03000 |
| 8871 | | |
| 8872 | | > key size 0.21000,0.03000 |
| 8873 | | |
| 8874 | | > key size 0.20000,0.03000 |
| 8875 | | |
| 8876 | | > key size 0.19000,0.03000 |
| 8877 | | |
| 8878 | | > key size 0.18000,0.03000 |
| 8879 | | |
| 8880 | | > key size 0.17000,0.03000 |
| 8881 | | |
| 8882 | | > key size 0.16000,0.03000 |
| 8883 | | |
| 8884 | | > key size 0.15000,0.03000 |
| 8885 | | |
| 8886 | | > key size 0.16000,0.03000 |
| 8887 | | |
| 8888 | | > key size 0.17000,0.03000 |
| 8889 | | |
| 8890 | | > key size 0.17000,0.04000 |
| 8891 | | |
| 8892 | | > key size 0.17000,0.05000 |
| 8893 | | |
| 8894 | | > key size 0.17000,0.04000 |
| 8895 | | |
| 8896 | | > key pos 0.36134,0.205524 size 0.0323267,0.199875 |
| 8897 | | |
| 8898 | | > key pos 0.375585,0.243532 size 0.000780577,0.00120384 |
| 8899 | | |
| 8900 | | > key pos 0.393081,0.282969 size 0,0 |
| 8901 | | |
| 8902 | | [Repeated 1 time(s)] |
| 8903 | | |
| 8904 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 8905 | | |
| 8906 | | > hide #!39 models |
| 8907 | | |
| 8908 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 8909 | | |
| 8910 | | > ui mousemode right "color key" |
| 8911 | | |
| 8912 | | > key pos 0.654167,0.35194 size 0.0389145,0.208023 |
| 8913 | | |
| 8914 | | > show #!39 models |
| 8915 | | |
| 8916 | | > key pos 0.434243,0.168612 size 0.0379186,0.161115 |
| 8917 | | |
| 8918 | | > hide #41 models |
| 8919 | | |
| 8920 | | > ui mousemode right "translate selected models" |
| 8921 | | |
| 8922 | | > ui mousemode right translate |
| 8923 | | |
| 8924 | | [Repeated 1 time(s)] |
| 8925 | | |
| 8926 | | > select add #41 |
| 8927 | | |
| 8928 | | 1 model selected |
| 8929 | | |
| 8930 | | > select subtract #41 |
| 8931 | | |
| 8932 | | Nothing selected |
| 8933 | | |
| 8934 | | > show #41 models |
| 8935 | | |
| 8936 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 8937 | | |
| 8938 | | > ui mousemode right select |
| 8939 | | |
| 8940 | | > select clear |
| 8941 | | |
| 8942 | | > ui mousemode right rotate |
| 8943 | | |
| 8944 | | > ui mousemode right translate |
| 8945 | | |
| 8946 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 8947 | | |
| 8948 | | [Repeated 1 time(s)] |
| 8949 | | |
| 8950 | | > ui mousemode right zoom |
| 8951 | | |
| 8952 | | > ui mousemode right "translate selected models" |
| 8953 | | |
| 8954 | | > ui mousemode right select |
| 8955 | | |
| 8956 | | > select clear |
| 8957 | | |
| 8958 | | > select add #41 |
| 8959 | | |
| 8960 | | 1 model selected |
| 8961 | | |
| 8962 | | > select clear |
| 8963 | | |
| 8964 | | Drag select of 39 cryosparc_P4_J285_006_volume_map_sharp.mrc z flip |
| 8965 | | |
| 8966 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 8967 | | |
| 8968 | | > select clear |
| 8969 | | |
| 8970 | | Drag select of 39 cryosparc_P4_J285_006_volume_map_sharp.mrc z flip |
| 8971 | | |
| 8972 | | > ui mousemode right translate |
| 8973 | | |
| 8974 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 8975 | | |
| 8976 | | > select subtract #41 |
| 8977 | | |
| 8978 | | 2 models selected |
| 8979 | | |
| 8980 | | > ui mousemode right "translate selected models" |
| 8981 | | |
| 8982 | | > ui mousemode right rotate |
| 8983 | | |
| 8984 | | > ui mousemode right translate |
| 8985 | | |
| 8986 | | > hide #!39 models |
| 8987 | | |
| 8988 | | > show #!39 models |
| 8989 | | |
| 8990 | | > ui mousemode right select |
| 8991 | | |
| 8992 | | > select #39 |
| 8993 | | |
| 8994 | | 2 models selected |
| 8995 | | |
| 8996 | | > ui mousemode right rotate |
| 8997 | | |
| 8998 | | > ui mousemode right translate |
| 8999 | | |
| 9000 | | > select subtract #39 |
| 9001 | | |
| 9002 | | Nothing selected |
| 9003 | | |
| 9004 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 9005 | | |
| 9006 | | > hide #!39 models |
| 9007 | | |
| 9008 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 9009 | | |
| 9010 | | > ui mousemode left "color key" |
| 9011 | | |
| 9012 | | > ui mousemode left rotate |
| 9013 | | |
| 9014 | | > ui mousemode right 'color key' |
| 9015 | | |
| 9016 | | > key pos 0.683937,0.290603 size 0.0366198,0.178803 |
| 9017 | | |
| 9018 | | > show #!39 models |
| 9019 | | |
| 9020 | | > key pos 0.885363,0.663755 size 0.0185522,0.00687018 |
| 9021 | | |
| 9022 | | > ui mousemode right translate |
| 9023 | | |
| 9024 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 9025 | | |
| 9026 | | > ui mousemode right "color key" |
| 9027 | | |
| 9028 | | > key pos 0.768821,0.24433 size 0.0347996,0.266198 |
| 9029 | | |
| 9030 | | > key labelOffset 7.0 |
| 9031 | | |
| 9032 | | > key labelOffset 8.0 |
| 9033 | | |
| 9034 | | > key labelOffset 9.0 |
| 9035 | | |
| 9036 | | > hide #!39 models |
| 9037 | | |
| 9038 | | > show #!37 models |
| 9039 | | |
| 9040 | | > volume #37 level 0.06504 |
| 9041 | | |
| 9042 | | > save "/Users/peiyuyu/Documents/Goethe |
| 9043 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 9044 | | > F_B1/20260503_SA-F_B1_workflow2.cxs" |
| 9045 | | |
| 9046 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 9047 | | |
| 9048 | | > ui mousemode right "color key" |
| 9049 | | |
| 9050 | | > key fontSize 25 |
| 9051 | | |
| 9052 | | > key fontSize 26 |
| 9053 | | |
| 9054 | | > key fontSize 27 |
| 9055 | | |
| 9056 | | > key fontSize 28 |
| 9057 | | |
| 9058 | | > key fontSize 29 |
| 9059 | | |
| 9060 | | > key fontSize 30 |
| 9061 | | |
| 9062 | | > key fontSize 31 |
| 9063 | | |
| 9064 | | > key fontSize 32 |
| 9065 | | |
| 9066 | | > key fontSize 33 |
| 9067 | | |
| 9068 | | > key fontSize 34 |
| 9069 | | |
| 9070 | | > key fontSize 35 |
| 9071 | | |
| 9072 | | > save CS_P4_J282_locres_sharp.png supersample 3 transparentBackground true |
| 9073 | | |
| 9074 | | > hide #!37 models |
| 9075 | | |
| 9076 | | > show #!39 models |
| 9077 | | |
| 9078 | | > volume #39 level 0.06897 |
| 9079 | | |
| 9080 | | > volume #39 level 0.06638 |
| 9081 | | |
| 9082 | | > save CS_P4_J285_locres_sharp.png supersample 3 transparentBackground true |
| 9083 | | |
| 9084 | | > save "/Users/peiyuyu/Documents/Goethe |
| 9085 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 9086 | | > F_B1/20260503_SA-F_B1_workflow2.cxs" |
| 9087 | | |
| 9088 | | > hide #!39 models |
| 9089 | | |
| 9090 | | > hide #41 models |
| 9091 | | |
| 9092 | | > open "/Users/peiyuyu/Documents/Goethe |
| 9093 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 9094 | | > F_B1/CS_P4_J282_sharp_flip.mrc" |
| 9095 | | |
| 9096 | | Opened CS_P4_J282_sharp_flip.mrc as #42, grid size 320,320,320, pixel 0.831, |
| 9097 | | shown at level 0.0392, step 2, values float32 |
| 9098 | | |
| 9099 | | > open "/Users/peiyuyu/Documents/Goethe University/19. Model Building/2. AcrF |
| 9100 | | > Project/2. Bound-form/SA-F_B1/Model/aligned_model_for_J282.pdb" |
| 9101 | | |
| 9102 | | Chain information for aligned_model_for_J282.pdb #43 |
| 9103 | | --- |
| 9104 | | Chain | Description |
| 9105 | | A B C | No description available |
| 9106 | | |
| 9107 | | |
| 9108 | | > hide #!42 models |
| 9109 | | |
| 9110 | | > select #43/BC |
| 9111 | | |
| 9112 | | Nothing selected |
| 9113 | | |
| 9114 | | > select #43/B, C |
| 9115 | | |
| 9116 | | 15672 atoms, 15966 bonds, 2068 residues, 1 model selected |
| 9117 | | |
| 9118 | | > delete sel |
| 9119 | | |
| 9120 | | > show #!42 models |
| 9121 | | |
| 9122 | | > volume zone #42 nearAtoms #43/A |
| 9123 | | |
| 9124 | | > color #43 #FFF6C0 |
| 9125 | | |
| 9126 | | > volume zone #42 nearAtoms #43/A distance 5 |
| 9127 | | |
| 9128 | | Expected a keyword |
| 9129 | | |
| 9130 | | > color zone #42 near #43/A distance 5 |
| 9131 | | |
| 9132 | | > color zone #42 near #43/A distance 5 tran 50 |
| 9133 | | |
| 9134 | | Expected a keyword |
| 9135 | | |
| 9136 | | > color zone #42 near #43/A distance 5 tran 50 |
| 9137 | | |
| 9138 | | Expected a keyword |
| 9139 | | |
| 9140 | | > color #43 #FFF6C0 |
| 9141 | | |
| 9142 | | > color zone #42 near #43/A distance 5 |
| 9143 | | |
| 9144 | | > color #43 #FFF6C0 transparency 50 |
| 9145 | | |
| 9146 | | > color zone #42 near #43/A distance 5 |
| 9147 | | |
| 9148 | | > graphics silhouettes false |
| 9149 | | |
| 9150 | | > lighting full |
| 9151 | | |
| 9152 | | > lighting flat |
| 9153 | | |
| 9154 | | [Repeated 1 time(s)] |
| 9155 | | |
| 9156 | | > lighting shadows true intensity 0.5 |
| 9157 | | |
| 9158 | | > lighting shadows false |
| 9159 | | |
| 9160 | | > lighting soft |
| 9161 | | |
| 9162 | | [Repeated 1 time(s)] |
| 9163 | | |
| 9164 | | > lighting simple |
| 9165 | | |
| 9166 | | > graphics silhouettes false |
| 9167 | | |
| 9168 | | > color #42 #99bfe580 models |
| 9169 | | |
| 9170 | | > hide #43 models |
| 9171 | | |
| 9172 | | > transparency #42 50 |
| 9173 | | |
| 9174 | | > lighting soft |
| 9175 | | |
| 9176 | | > volume #42 level 0.0232 |
| 9177 | | |
| 9178 | | > lighting soft |
| 9179 | | |
| 9180 | | > close #42 |
| 9181 | | |
| 9182 | | > close #43 |
| 9183 | | |
| 9184 | | > open "/Users/peiyuyu/Documents/Goethe |
| 9185 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 9186 | | > F_B1/cryosparc_P4_J242_class_02_00122_volume_sharp.mrc" |
| 9187 | | |
| 9188 | | Opened cryosparc_P4_J242_class_02_00122_volume_sharp.mrc as #36, grid size |
| 9189 | | 128,128,128, pixel 2.08, shown at level 0.195, step 1, values float32 |
| 9190 | | |
| 9191 | | > open "/Users/peiyuyu/Documents/Goethe |
| 9192 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 9193 | | > F_B1/cryosparc_P4_J242_class_01_00122_volume_sharp.mrc" |
| 9194 | | |
| 9195 | | Opened cryosparc_P4_J242_class_01_00122_volume_sharp.mrc as #42, grid size |
| 9196 | | 128,128,128, pixel 2.08, shown at level 0.178, step 1, values float32 |
| 9197 | | |
| 9198 | | > close #36 |
| 9199 | | |
| 9200 | | > show #!9 models |
| 9201 | | |
| 9202 | | > select add #42 |
| 9203 | | |
| 9204 | | 2 models selected |
| 9205 | | |
| 9206 | | > ui mousemode right "rotate selected models" |
| 9207 | | |
| 9208 | | > view matrix models |
| 9209 | | > #42,0.026241,-0.92593,0.37678,198.52,-0.063177,0.37462,0.92502,-34.721,-0.99766,-0.048078,-0.048667,279.22 |
| 9210 | | |
| 9211 | | > ui mousemode right "translate selected models" |
| 9212 | | |
| 9213 | | > view matrix models |
| 9214 | | > #42,0.026241,-0.92593,0.37678,198.7,-0.063177,0.37462,0.92502,-33.267,-0.99766,-0.048078,-0.048667,263 |
| 9215 | | |
| 9216 | | > fitmap #42 inMap #9 |
| 9217 | | |
| 9218 | | Fit map cryosparc_P4_J242_class_01_00122_volume_sharp.mrc in map |
| 9219 | | cryosparc_P4_J242_class_01_00122_volume_sharp.mrc using 20960 points |
| 9220 | | correlation = 1, correlation about mean = 1, overlap = 1571 |
| 9221 | | steps = 128, shift = 5.08, angle = 27.9 degrees |
| 9222 | | |
| 9223 | | Position of cryosparc_P4_J242_class_01_00122_volume_sharp.mrc (#42) relative |
| 9224 | | to cryosparc_P4_J242_class_01_00122_volume_sharp.mrc (#9) coordinates: |
| 9225 | | Matrix rotation and translation |
| 9226 | | 0.99999999 -0.00010909 0.00000609 0.02020954 |
| 9227 | | 0.00010909 0.99999995 0.00031305 -0.06272325 |
| 9228 | | -0.00000612 -0.00031305 0.99999995 0.03217050 |
| 9229 | | Axis -0.94414385 0.01842055 0.32901836 |
| 9230 | | Axis point 0.00000000 112.87704178 199.81299213 |
| 9231 | | Rotation angle (degrees) 0.01899762 |
| 9232 | | Shift along axis -0.00965143 |
| 9233 | | |
| 9234 | | |
| 9235 | | > select subtract #42 |
| 9236 | | |
| 9237 | | Nothing selected |
| 9238 | | |
| 9239 | | > select add #42 |
| 9240 | | |
| 9241 | | 2 models selected |
| 9242 | | |
| 9243 | | > fitmap #42 inMap #9 |
| 9244 | | |
| 9245 | | Fit map cryosparc_P4_J242_class_01_00122_volume_sharp.mrc in map |
| 9246 | | cryosparc_P4_J242_class_01_00122_volume_sharp.mrc using 20960 points |
| 9247 | | correlation = 1, correlation about mean = 1, overlap = 1571 |
| 9248 | | steps = 40, shift = 0.00318, angle = 0.00769 degrees |
| 9249 | | |
| 9250 | | Position of cryosparc_P4_J242_class_01_00122_volume_sharp.mrc (#42) relative |
| 9251 | | to cryosparc_P4_J242_class_01_00122_volume_sharp.mrc (#9) coordinates: |
| 9252 | | Matrix rotation and translation |
| 9253 | | 0.99999999 -0.00011772 0.00012933 0.00283694 |
| 9254 | | 0.00011769 0.99999996 0.00026066 -0.05912201 |
| 9255 | | -0.00012937 -0.00026064 0.99999996 0.04294746 |
| 9256 | | Axis -0.83040251 0.41209275 0.37498166 |
| 9257 | | Axis point 0.00000000 180.01203882 210.06369623 |
| 9258 | | Rotation angle (degrees) 0.01798432 |
| 9259 | | Shift along axis -0.01061505 |
| 9260 | | |
| 9261 | | |
| 9262 | | > select subtract #42 |
| 9263 | | |
| 9264 | | Nothing selected |
| 9265 | | |
| 9266 | | > hide #!9 models |
| 9267 | | |
| 9268 | | > volume #42 level 0.2579 |
| 9269 | | |
| 9270 | | > color #42 #ffffb2ff models |
| 9271 | | |
| 9272 | | > color #42 #ffffb280 models |
| 9273 | | |
| 9274 | | > open "/Users/peiyuyu/Documents/Goethe |
| 9275 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 9276 | | > F_B1/CS_P4_J282_sharp_flip.mrc" |
| 9277 | | |
| 9278 | | Opened CS_P4_J282_sharp_flip.mrc as #36, grid size 320,320,320, pixel 0.831, |
| 9279 | | shown at level 0.0392, step 2, values float32 |
| 9280 | | |
| 9281 | | > volume #42 level 0.1954 |
| 9282 | | |
| 9283 | | > select add #42 |
| 9284 | | |
| 9285 | | 2 models selected |
| 9286 | | |
| 9287 | | > view matrix models |
| 9288 | | > #42,0.15189,-0.98482,-0.084022,248.42,-0.056394,-0.093504,0.99402,24.018,-0.98679,-0.14625,-0.069741,273.86 |
| 9289 | | |
| 9290 | | > volume #42 level 0.2537 |
| 9291 | | |
| 9292 | | > open "/Users/peiyuyu/Documents/Goethe |
| 9293 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 9294 | | > F_B1/cryosparc_P4_J169_class_03_00182_volume_sharp.mrc" |
| 9295 | | |
| 9296 | | Opened cryosparc_P4_J169_class_03_00182_volume_sharp.mrc as #43, grid size |
| 9297 | | 128,128,128, pixel 2.08, shown at level 0.248, step 1, values float32 |
| 9298 | | |
| 9299 | | > close #43 |
| 9300 | | |
| 9301 | | > open "/Users/peiyuyu/Documents/Goethe |
| 9302 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 9303 | | > F_B1/cryosparc_P4_J169_class_02_00182_volume_sharp.mrc" |
| 9304 | | |
| 9305 | | Opened cryosparc_P4_J169_class_02_00182_volume_sharp.mrc as #43, grid size |
| 9306 | | 128,128,128, pixel 2.08, shown at level 0.216, step 1, values float32 |
| 9307 | | |
| 9308 | | > select add #43 |
| 9309 | | |
| 9310 | | 4 models selected |
| 9311 | | |
| 9312 | | > view matrix models |
| 9313 | | > #42,0.15189,-0.98482,-0.084022,246.73,-0.056394,-0.093504,0.99402,-45.124,-0.98679,-0.14625,-0.069741,235.92,#43,1,0,0,-1.6882,0,1,0,-69.141,0,0,1,-37.947 |
| 9314 | | |
| 9315 | | > view matrix models |
| 9316 | | > #42,0.15189,-0.98482,-0.084022,237,-0.056394,-0.093504,0.99402,126.12,-0.98679,-0.14625,-0.069741,312.08,#43,1,0,0,-11.419,0,1,0,102.11,0,0,1,38.221 |
| 9317 | | |
| 9318 | | > select subtract #42 |
| 9319 | | |
| 9320 | | 2 models selected |
| 9321 | | |
| 9322 | | > view matrix models #43,1,0,0,-10.922,0,1,0,105.75,0,0,1,25.23 |
| 9323 | | |
| 9324 | | > ui mousemode right "rotate selected models" |
| 9325 | | |
| 9326 | | > view matrix models |
| 9327 | | > #43,0.053361,-0.96815,-0.24461,270.41,-0.10024,-0.24891,0.96332,141.36,-0.99353,-0.026885,-0.11033,315.47 |
| 9328 | | |
| 9329 | | > ui mousemode right "translate selected models" |
| 9330 | | |
| 9331 | | > view matrix models |
| 9332 | | > #43,0.053361,-0.96815,-0.24461,276.43,-0.10024,-0.24891,0.96332,50.344,-0.99353,-0.026885,-0.11033,257.37 |
| 9333 | | |
| 9334 | | > ui mousemode right "rotate selected models" |
| 9335 | | |
| 9336 | | > view matrix models |
| 9337 | | > #43,0.25016,-0.91768,-0.30868,252.37,-0.0074281,-0.32063,0.94718,48.872,-0.96818,-0.23465,-0.087026,276.42 |
| 9338 | | |
| 9339 | | > ui mousemode right "translate selected models" |
| 9340 | | |
| 9341 | | > view matrix models |
| 9342 | | > #43,0.25016,-0.91768,-0.30868,252.17,-0.0074281,-0.32063,0.94718,49.119,-0.96818,-0.23465,-0.087026,280.28 |
| 9343 | | |
| 9344 | | > view matrix models |
| 9345 | | > #43,0.25016,-0.91768,-0.30868,245.18,-0.0074281,-0.32063,0.94718,41.479,-0.96818,-0.23465,-0.087026,278.46 |
| 9346 | | |
| 9347 | | > view matrix models |
| 9348 | | > #43,0.25016,-0.91768,-0.30868,244.24,-0.0074281,-0.32063,0.94718,46.881,-0.96818,-0.23465,-0.087026,278.05 |
| 9349 | | |
| 9350 | | > select add #42 |
| 9351 | | |
| 9352 | | 4 models selected |
| 9353 | | |
| 9354 | | > select subtract #43 |
| 9355 | | |
| 9356 | | 2 models selected |
| 9357 | | |
| 9358 | | > view matrix models |
| 9359 | | > #42,0.15189,-0.98482,-0.084022,248.26,-0.056394,-0.093504,0.99402,10.768,-0.98679,-0.14625,-0.069741,272.27 |
| 9360 | | |
| 9361 | | > select subtract #42 |
| 9362 | | |
| 9363 | | Nothing selected |
| 9364 | | |
| 9365 | | > color #36 #99BFE5 |
| 9366 | | |
| 9367 | | > hide #!42 models |
| 9368 | | |
| 9369 | | > select add #43 |
| 9370 | | |
| 9371 | | 2 models selected |
| 9372 | | |
| 9373 | | > ui mousemode right "rotate selected models" |
| 9374 | | |
| 9375 | | > view matrix models |
| 9376 | | > #43,0.073784,-0.55439,0.82898,66.918,0.0052521,0.83145,0.55558,-43.45,-0.99726,-0.036639,0.064259,236.72 |
| 9377 | | |
| 9378 | | > ui mousemode right "translate selected models" |
| 9379 | | |
| 9380 | | > view matrix models |
| 9381 | | > #43,0.073784,-0.55439,0.82898,82.905,0.0052521,0.83145,0.55558,-39.115,-0.99726,-0.036639,0.064259,236.78 |
| 9382 | | |
| 9383 | | > select subtract #43 |
| 9384 | | |
| 9385 | | Nothing selected |
| 9386 | | |
| 9387 | | > volume #36 level 0.0602 |
| 9388 | | |
| 9389 | | > select add #43 |
| 9390 | | |
| 9391 | | 2 models selected |
| 9392 | | |
| 9393 | | > view matrix models |
| 9394 | | > #43,0.073784,-0.55439,0.82898,84.941,0.0052521,0.83145,0.55558,-38.546,-0.99726,-0.036639,0.064259,236.53 |
| 9395 | | |
| 9396 | | > view matrix models |
| 9397 | | > #43,0.073784,-0.55439,0.82898,84.833,0.0052521,0.83145,0.55558,-38.074,-0.99726,-0.036639,0.064259,236.34 |
| 9398 | | |
| 9399 | | > volume #43 level 0.1902 |
| 9400 | | |
| 9401 | | > color #43 #b2b2ff80 models |
| 9402 | | |
| 9403 | | > volume #43 level 0.2283 |
| 9404 | | |
| 9405 | | > select add #40 |
| 9406 | | |
| 9407 | | 4 models selected |
| 9408 | | |
| 9409 | | > select subtract #40 |
| 9410 | | |
| 9411 | | 2 models selected |
| 9412 | | |
| 9413 | | > select subtract #43 |
| 9414 | | |
| 9415 | | Nothing selected |
| 9416 | | |
| 9417 | | > color #43 #ffffb280 models |
| 9418 | | |
| 9419 | | > color #43 #b2b2ff80 models |
| 9420 | | |
| 9421 | | > save "/Users/peiyuyu/Documents/Goethe |
| 9422 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 9423 | | > F_B1/20260503_SA-F_B1_workflow3.cxs" |
| 9424 | | |
| 9425 | | > open "/Users/peiyuyu/Documents/Goethe |
| 9426 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 9427 | | > F_B1/cryosparc_P4_cryosparc_P4_J243_003_volume_map_35_TMD_regions_resampled.mrc" |
| 9428 | | |
| 9429 | | Opened |
| 9430 | | cryosparc_P4_cryosparc_P4_J243_003_volume_map_35_TMD_regions_resampled.mrc as |
| 9431 | | #44, grid size 80,80,80, pixel 3.32, shown at level 0.324, step 1, values |
| 9432 | | float32 |
| 9433 | | |
| 9434 | | > close #44 |
| 9435 | | |
| 9436 | | > save CS_P4_J282_show_mask_sharp.png supersample 3 transparentBackground true |
| 9437 | | |
| 9438 | | > save "/Users/peiyuyu/Documents/Goethe |
| 9439 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 9440 | | > F_B1/20260503_SA-F_B1_workflow4.cxs" |
| 9441 | | |
| 9442 | | ——— End of log from Sun May 3 17:29:06 2026 ——— |
| 9443 | | |
| 9444 | | > view name session-start |
| 9445 | | |
| 9446 | | opened ChimeraX session |
| 9447 | | |
| 9448 | | > open "/Users/peiyuyu/Documents/Goethe |
| 9449 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 9450 | | > F_B1/cryosparc_P4_J295_006_volume_map_sharp.mrc" |
| 9451 | | |
| 9452 | | Opened cryosparc_P4_J295_006_volume_map_sharp.mrc as #44, grid size |
| 9453 | | 320,320,320, pixel 0.831, shown at level 0.0361, step 2, values float32 |
| 9454 | | |
| 9455 | | > open "/Users/peiyuyu/Documents/Goethe |
| 9456 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 9457 | | > F_B1/cryosparc_P4_J296_map_locres.mrc" |
| 9458 | | |
| 9459 | | Opened cryosparc_P4_J296_map_locres.mrc as #45, grid size 320,320,320, pixel |
| 9460 | | 0.831, shown at level 8.66, step 2, values float32 |
| 9461 | | |
| 9462 | | > hide #!43 models |
| 9463 | | |
| 9464 | | > hide #!36 models |
| 9465 | | |
| 9466 | | > hide #!45 models |
| 9467 | | |
| 9468 | | > show #!45 models |
| 9469 | | |
| 9470 | | > hide #!45 models |
| 9471 | | |
| 9472 | | > volume flip #44 |
| 9473 | | |
| 9474 | | Opened cryosparc_P4_J295_006_volume_map_sharp.mrc z flip as #46, grid size |
| 9475 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 9476 | | |
| 9477 | | > volume flip #45 |
| 9478 | | |
| 9479 | | Opened cryosparc_P4_J296_map_locres.mrc z flip as #47, grid size 320,320,320, |
| 9480 | | pixel 0.831, shown at step 1, values float32 |
| 9481 | | |
| 9482 | | > close #45 |
| 9483 | | |
| 9484 | | > close #44 |
| 9485 | | |
| 9486 | | > rename #46 CS_P4_J295_sharp_flip id #44 |
| 9487 | | |
| 9488 | | > save CS_P4_J295_sharp_flip.mrc #44 |
| 9489 | | |
| 9490 | | > rename #47 CS_P4_J296_locres_flip id #45 |
| 9491 | | |
| 9492 | | > save CS_P4_J296_locres_flip.mrc #45 |
| 9493 | | |
| 9494 | | > ui tool show "Surface Color" |
| 9495 | | |
| 9496 | | > hide #!45 models |
| 9497 | | |
| 9498 | | > color sample #44.1 map #45 palette #ffb17f:#527dea:#99bfe5:#fff6c0:#f4fff3 |
| 9499 | | > outsideColor #808080 |
| 9500 | | |
| 9501 | | Map values for surface "surface": minimum 0, mean 4.132, maximum 48.35 |
| 9502 | | |
| 9503 | | > show #41 models |
| 9504 | | |
| 9505 | | > volume #44 level 0.0515 |
| 9506 | | |
| 9507 | | > color sample #44.1 map #45 palette |
| 9508 | | > 1,#ffb17f:2,#527dea:3,#99bfe5:4,#fff6c0:5,#f4fff3 outsideColor #808080 |
| 9509 | | |
| 9510 | | Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22 |
| 9511 | | |
| 9512 | | > color sample #44.1 map #45 palette |
| 9513 | | > 2,#ffb17f:3,#527dea:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 9514 | | |
| 9515 | | Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22 |
| 9516 | | |
| 9517 | | > color sample #44.1 map #45 palette |
| 9518 | | > 2,#ffb17f:3,#527dea:4,#ffb17f:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 9519 | | |
| 9520 | | Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22 |
| 9521 | | |
| 9522 | | > color sample #44.1 map #45 palette |
| 9523 | | > 2,#527dea:3,#99bfe5:4,#ffb17f:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 9524 | | |
| 9525 | | Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22 |
| 9526 | | |
| 9527 | | > key #527dea:2 #99bfe5:3 #ffb17f:4 #fff6c0:5 #f4fff3:6 showTool true |
| 9528 | | |
| 9529 | | > ui mousemode right "color key" |
| 9530 | | |
| 9531 | | > color sample #44.1 map #45 palette |
| 9532 | | > 2,#f4fff3:3,#fff6c0:4,#ffb17f:5,#99bfe5:6,#527dea outsideColor #808080 |
| 9533 | | |
| 9534 | | Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22 |
| 9535 | | |
| 9536 | | > key #f4fff3:2 #fff6c0:3 #ffb17f:4 #99bfe5:5 #527dea:6 showTool true |
| 9537 | | |
| 9538 | | > ui mousemode right "color key" |
| 9539 | | |
| 9540 | | > color sample #44.1 map #45 palette |
| 9541 | | > 2,#f4fff3:3,#fff6c0:4,#ffb17f:5,#99bfe5:6,#527dd6 outsideColor #808080 |
| 9542 | | |
| 9543 | | Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22 |
| 9544 | | |
| 9545 | | > color sample #44.1 map #45 palette |
| 9546 | | > 2,#f4fff3:3,#fff6c0:4,#ffb17f:5,#99bfe5:6,#46a2de outsideColor #808080 |
| 9547 | | |
| 9548 | | Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22 |
| 9549 | | |
| 9550 | | > color sample #44.1 map #45 palette |
| 9551 | | > 2,#f4fff3:3,#fff6c0:4,#ffb17f:5,#99bfe5:6,#4d8fde outsideColor #808080 |
| 9552 | | |
| 9553 | | Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22 |
| 9554 | | |
| 9555 | | > color sample #44.1 map #45 palette |
| 9556 | | > 2,#f4fff3:3,#fff6c0:4,#ffb17f:5,#99bfe5:6,#94aec1 outsideColor #808080 |
| 9557 | | |
| 9558 | | Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22 |
| 9559 | | |
| 9560 | | > color sample #44.1 map #45 palette |
| 9561 | | > 2,#f4fff3:3,#fff6c0:4,#ffb17f:5,#99bfe5:6,#a78bc1 outsideColor #808080 |
| 9562 | | |
| 9563 | | Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22 |
| 9564 | | |
| 9565 | | > color sample #44.1 map #45 palette |
| 9566 | | > 2,#f4fff3:3,#fff6c0:4,#ffb17f:5,#99bfe5:6,#c1a77f outsideColor #808080 |
| 9567 | | |
| 9568 | | Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22 |
| 9569 | | |
| 9570 | | > color sample #44.1 map #45 palette |
| 9571 | | > 2,#f4fff3:3,#fff6c0:4,#ffb17f:5,#99bfe5:6,#89b3c1 outsideColor #808080 |
| 9572 | | |
| 9573 | | Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22 |
| 9574 | | |
| 9575 | | > color sample #44.1 map #45 palette |
| 9576 | | > 2,#ffb17f:3,#527dea:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 9577 | | |
| 9578 | | Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22 |
| 9579 | | |
| 9580 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 9581 | | |
| 9582 | | > ui mousemode right "color key" |
| 9583 | | |
| 9584 | | > color sample #44.1 map #45 palette |
| 9585 | | > 2,#ffb17f:3,#2c98ee:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 9586 | | |
| 9587 | | Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22 |
| 9588 | | |
| 9589 | | > color sample #44.1 map #45 palette |
| 9590 | | > 2,#ffb17f:3,#4979d3:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 9591 | | |
| 9592 | | Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22 |
| 9593 | | |
| 9594 | | > save CS_P4_J295_locres_sharp.png supersample 3 transparentBackground true |
| 9595 | | |
| 9596 | | Cell requested for row 0 is out of bounds for table with 6 rows! Resizing |
| 9597 | | table model. |
| 9598 | | |
| 9599 | | > save "/Users/peiyuyu/Documents/Goethe |
| 9600 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 9601 | | > F_B1/20260505_SA-F_B1_workflow.cxs" |
| 9602 | | |
| 9603 | | > open "/Users/peiyuyu/Documents/Goethe |
| 9604 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 9605 | | > F_B1/cryosparc_P4_J300_005_volume_map_sharp.mrc" |
| 9606 | | |
| 9607 | | Opened cryosparc_P4_J300_005_volume_map_sharp.mrc as #46, grid size |
| 9608 | | 320,320,320, pixel 0.831, shown at level 0.0375, step 2, values float32 |
| 9609 | | |
| 9610 | | > open "/Users/peiyuyu/Documents/Goethe |
| 9611 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 9612 | | > F_B1/cryosparc_P4_J301_map_locres.mrc" |
| 9613 | | |
| 9614 | | Opened cryosparc_P4_J301_map_locres.mrc as #47, grid size 320,320,320, pixel |
| 9615 | | 0.831, shown at level 9.68, step 2, values float32 |
| 9616 | | |
| 9617 | | > hide #!44 models |
| 9618 | | |
| 9619 | | > hide #!47 models |
| 9620 | | |
| 9621 | | > volume flip #46 |
| 9622 | | |
| 9623 | | Opened cryosparc_P4_J300_005_volume_map_sharp.mrc z flip as #48, grid size |
| 9624 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 9625 | | |
| 9626 | | > volume flip #47 |
| 9627 | | |
| 9628 | | Opened cryosparc_P4_J301_map_locres.mrc z flip as #49, grid size 320,320,320, |
| 9629 | | pixel 0.831, shown at step 1, values float32 |
| 9630 | | |
| 9631 | | > close #46 |
| 9632 | | |
| 9633 | | > close #47 |
| 9634 | | |
| 9635 | | > rename #48 CS_P4_J300_sharp_flip id #46 |
| 9636 | | |
| 9637 | | > rename #49 CS_P4_J301_locres_flip id #47 |
| 9638 | | |
| 9639 | | > save CS_P4_J301_locres_flip.mrc #47 |
| 9640 | | |
| 9641 | | > save CS_P4_J300_sharp_flip.mrc #46 |
| 9642 | | |
| 9643 | | > color sample #46.1 map #47 palette |
| 9644 | | > 2,#ffb17f:3,#4979d3:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 9645 | | |
| 9646 | | Map values for surface "surface": minimum 0, mean 4.498, maximum 44.88 |
| 9647 | | |
| 9648 | | > hide #!47 models |
| 9649 | | |
| 9650 | | > volume #46 level 0.03399 |
| 9651 | | |
| 9652 | | > volume #46 level 0.06962 |
| 9653 | | |
| 9654 | | > volume #46 level 0.05919 |
| 9655 | | |
| 9656 | | > save CS_P4_J300_locres_sharp.png supersample 3 transparentBackground true |
| 9657 | | |
| 9658 | | > save "/Users/peiyuyu/Documents/Goethe |
| 9659 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 9660 | | > F_B1/20260505_SA-F_B1_workflow.cxs" |
| 9661 | | |
| 9662 | | ——— End of log from Tue May 5 09:31:57 2026 ——— |
| 9663 | | |
| 9664 | | > view name session-start |
| 9665 | | |
| 9666 | | opened ChimeraX session |
| 9667 | | |
| 9668 | | > open "/Users/peiyuyu/Documents/Goethe |
| 9669 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 9670 | | > F_B1/cryosparc_P4_J302_002_volume_map_sharp.mrc" |
| 9671 | | |
| 9672 | | Opened cryosparc_P4_J302_002_volume_map_sharp.mrc as #48, grid size 80,80,80, |
| 9673 | | pixel 3.32, shown at level 0.658, step 1, values float32 |
| 9674 | | |
| 9675 | | > select add #48 |
| 9676 | | |
| 9677 | | 2 models selected |
| 9678 | | |
| 9679 | | > ui mousemode right "rotate selected models" |
| 9680 | | |
| 9681 | | > view matrix models |
| 9682 | | > #48,0.064602,0.97911,0.19278,-26.266,0.98732,-0.034646,-0.1549,22.036,-0.14498,0.20034,-0.96894,229.94 |
| 9683 | | |
| 9684 | | > volume flip #48 |
| 9685 | | |
| 9686 | | Opened cryosparc_P4_J302_002_volume_map_sharp.mrc z flip as #49, grid size |
| 9687 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 9688 | | |
| 9689 | | > close #48 |
| 9690 | | |
| 9691 | | > rename #49 rename |
| 9692 | | |
| 9693 | | > rename #49 CS_P4_J302_sharp_flip id #48 |
| 9694 | | |
| 9695 | | > save CS_P4_J302_sharp_flip.mrc #48 |
| 9696 | | |
| 9697 | | > select add #48 |
| 9698 | | |
| 9699 | | 2 models selected |
| 9700 | | |
| 9701 | | > view matrix models |
| 9702 | | > #48,-0.56486,-0.82475,0.026783,324.58,0.77395,-0.54076,-0.32951,143.72,0.28625,-0.1654,0.94377,-52.222 |
| 9703 | | |
| 9704 | | > hide #41 models |
| 9705 | | |
| 9706 | | > hide #!46 models |
| 9707 | | |
| 9708 | | > show #!47 models |
| 9709 | | |
| 9710 | | > hide #!47 models |
| 9711 | | |
| 9712 | | > show #!45 models |
| 9713 | | |
| 9714 | | > hide #!45 models |
| 9715 | | |
| 9716 | | > show #!43 models |
| 9717 | | |
| 9718 | | > hide #!43 models |
| 9719 | | |
| 9720 | | > show #!39 models |
| 9721 | | |
| 9722 | | > ui mousemode right "translate selected models" |
| 9723 | | |
| 9724 | | > view matrix models |
| 9725 | | > #48,-0.56486,-0.82475,0.026783,319.51,0.77395,-0.54076,-0.32951,139.04,0.28625,-0.1654,0.94377,-3.8614 |
| 9726 | | |
| 9727 | | > view matrix models |
| 9728 | | > #48,-0.56486,-0.82475,0.026783,321.32,0.77395,-0.54076,-0.32951,140.03,0.28625,-0.1654,0.94377,-10.821 |
| 9729 | | |
| 9730 | | > fitmap $48 inmap #39 |
| 9731 | | |
| 9732 | | Missing or invalid "atomsOrMap" argument: invalid objects specifier |
| 9733 | | |
| 9734 | | > fitmap #48 inMap #39 |
| 9735 | | |
| 9736 | | Fit map CS_P4_J302_sharp_flip.mrc in map |
| 9737 | | cryosparc_P4_J285_006_volume_map_sharp.mrc z flip using 5112 points |
| 9738 | | correlation = 0.3608, correlation about mean = 0.02111, overlap = 103.8 |
| 9739 | | steps = 76, shift = 4.71, angle = 3.71 degrees |
| 9740 | | |
| 9741 | | Position of CS_P4_J302_sharp_flip.mrc (#48) relative to |
| 9742 | | cryosparc_P4_J285_006_volume_map_sharp.mrc z flip (#39) coordinates: |
| 9743 | | Matrix rotation and translation |
| 9744 | | -0.54436883 -0.83749052 0.04766738 317.13056079 |
| 9745 | | 0.79848148 -0.53475206 -0.27652762 129.04232256 |
| 9746 | | 0.25707950 -0.11247150 0.95982304 -11.58663120 |
| 9747 | | Axis 0.09898047 -0.12634523 0.98703584 |
| 9748 | | Axis point 124.28808276 149.04371086 0.00000000 |
| 9749 | | Rotation angle (degrees) 124.03152255 |
| 9750 | | Shift along axis 3.64942894 |
| 9751 | | |
| 9752 | | |
| 9753 | | > ui mousemode right "rotate selected models" |
| 9754 | | |
| 9755 | | > view matrix models |
| 9756 | | > #48,-0.38435,0.91427,-0.12797,86.018,-0.92117,-0.38898,-0.012326,307,-0.061046,0.11314,0.9917,-2.8083 |
| 9757 | | |
| 9758 | | > fitmap #48 inMap #39 |
| 9759 | | |
| 9760 | | Fit map CS_P4_J302_sharp_flip.mrc in map |
| 9761 | | cryosparc_P4_J285_006_volume_map_sharp.mrc z flip using 5112 points |
| 9762 | | correlation = 0.623, correlation about mean = 0.1973, overlap = 254 |
| 9763 | | steps = 104, shift = 5.55, angle = 8.62 degrees |
| 9764 | | |
| 9765 | | Position of CS_P4_J302_sharp_flip.mrc (#48) relative to |
| 9766 | | cryosparc_P4_J285_006_volume_map_sharp.mrc z flip (#39) coordinates: |
| 9767 | | Matrix rotation and translation |
| 9768 | | -0.49090927 0.85957769 -0.14189522 105.36638005 |
| 9769 | | -0.85910240 -0.50468247 -0.08508012 328.20339808 |
| 9770 | | -0.14474501 0.08013591 0.98621858 12.70754555 |
| 9771 | | Axis 0.09568836 0.00165051 -0.99540997 |
| 9772 | | Axis point 147.88121154 133.69042072 0.00000000 |
| 9773 | | Rotation angle (degrees) 120.31054935 |
| 9774 | | Shift along axis -2.02517664 |
| 9775 | | |
| 9776 | | |
| 9777 | | > select subtract #48 |
| 9778 | | |
| 9779 | | Nothing selected |
| 9780 | | |
| 9781 | | > volume #48 level 0.8066 |
| 9782 | | |
| 9783 | | > hide #!48 models |
| 9784 | | |
| 9785 | | > show #!48 models |
| 9786 | | |
| 9787 | | > hide #!39 models |
| 9788 | | |
| 9789 | | > show #!40 models |
| 9790 | | |
| 9791 | | > hide #!40 models |
| 9792 | | |
| 9793 | | > show #!46 models |
| 9794 | | |
| 9795 | | > hide #!46 models |
| 9796 | | |
| 9797 | | > show #!46 models |
| 9798 | | |
| 9799 | | > hide #!46 models |
| 9800 | | |
| 9801 | | > volume #48 level 0.6121 |
| 9802 | | |
| 9803 | | > show #!24 models |
| 9804 | | |
| 9805 | | > hide #!24 models |
| 9806 | | |
| 9807 | | > color #48 #99BFE5 |
| 9808 | | |
| 9809 | | > hide #!48 models |
| 9810 | | |
| 9811 | | > show #!48 models |
| 9812 | | |
| 9813 | | > open "/Users/peiyuyu/Documents/Goethe |
| 9814 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 9815 | | > F_B1/cryosparc_P4_J303_003_volume_map_sharp.mrc" |
| 9816 | | |
| 9817 | | Opened cryosparc_P4_J303_003_volume_map_sharp.mrc as #49, grid size 80,80,80, |
| 9818 | | pixel 3.32, shown at level 0.57, step 1, values float32 |
| 9819 | | |
| 9820 | | > select add #49 |
| 9821 | | |
| 9822 | | 2 models selected |
| 9823 | | |
| 9824 | | > view matrix models |
| 9825 | | > #49,0.53874,0.84077,0.053573,-61.438,0.83514,-0.54134,0.097405,87.116,0.1109,-0.0077344,-0.9938,260.56 |
| 9826 | | |
| 9827 | | > view matrix models |
| 9828 | | > #49,0.53215,0.84494,0.053856,-61.154,0.83957,-0.53484,0.095262,85.896,0.10929,-0.0054773,-0.99399,260.49 |
| 9829 | | |
| 9830 | | > view matrix models |
| 9831 | | > #49,-0.8763,-0.44891,0.17485,296.18,-0.45964,0.88777,-0.024369,82.131,-0.14428,-0.10172,-0.98429,307.41 |
| 9832 | | |
| 9833 | | > volume flip #49 |
| 9834 | | |
| 9835 | | Opened cryosparc_P4_J303_003_volume_map_sharp.mrc z flip as #50, grid size |
| 9836 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 9837 | | |
| 9838 | | > close #49 |
| 9839 | | |
| 9840 | | > rename #50 CS_P4_J303_sharp_flip id #49 |
| 9841 | | |
| 9842 | | > save CS_P4_J303_sharp_flip.mrc #49 |
| 9843 | | |
| 9844 | | > select add #49 |
| 9845 | | |
| 9846 | | 2 models selected |
| 9847 | | |
| 9848 | | > view matrix models |
| 9849 | | > #49,-0.44927,0.88595,-0.11509,87.376,-0.86051,-0.46376,-0.21085,349.13,-0.24018,0.0043123,0.97072,61.749 |
| 9850 | | |
| 9851 | | > ui mousemode right "translate selected models" |
| 9852 | | |
| 9853 | | > view matrix models |
| 9854 | | > #49,-0.44927,0.88595,-0.11509,93.572,-0.86051,-0.46376,-0.21085,352.06,-0.24018,0.0043123,0.97072,37.843 |
| 9855 | | |
| 9856 | | > fitmap #49 inMap 348 |
| 9857 | | |
| 9858 | | Invalid "inMap" argument: invalid density map specifier |
| 9859 | | |
| 9860 | | > fitmap #49 inMap #48 |
| 9861 | | |
| 9862 | | Fit map CS_P4_J303_sharp_flip.mrc in map CS_P4_J302_sharp_flip.mrc using 5118 |
| 9863 | | points |
| 9864 | | correlation = 0.8483, correlation about mean = 0.4386, overlap = 3011 |
| 9865 | | steps = 68, shift = 10.7, angle = 7.19 degrees |
| 9866 | | |
| 9867 | | Position of CS_P4_J303_sharp_flip.mrc (#49) relative to |
| 9868 | | CS_P4_J302_sharp_flip.mrc (#48) coordinates: |
| 9869 | | Matrix rotation and translation |
| 9870 | | 0.99996126 -0.00401528 0.00783497 -1.22617333 |
| 9871 | | 0.00399514 0.99998870 0.00258400 -1.26181412 |
| 9872 | | -0.00784526 -0.00255260 0.99996598 1.34185977 |
| 9873 | | Axis -0.28004899 0.85489100 0.43673098 |
| 9874 | | Axis point 178.64594040 0.00000000 162.72002408 |
| 9875 | | Rotation angle (degrees) 0.52546068 |
| 9876 | | Shift along axis -0.14929320 |
| 9877 | | |
| 9878 | | |
| 9879 | | > select subtract #49 |
| 9880 | | |
| 9881 | | Nothing selected |
| 9882 | | |
| 9883 | | > hide #!48 models |
| 9884 | | |
| 9885 | | > color #49 #FCEDFF |
| 9886 | | |
| 9887 | | > hide #!49 models |
| 9888 | | |
| 9889 | | > show #!49 models |
| 9890 | | |
| 9891 | | > volume #49 level 0.5753 |
| 9892 | | |
| 9893 | | > open "/Users/peiyuyu/Documents/Goethe |
| 9894 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 9895 | | > F_B1/cryosparc_P4_J304_002_volume_map_sharp.mrc" |
| 9896 | | |
| 9897 | | Opened cryosparc_P4_J304_002_volume_map_sharp.mrc as #50, grid size 80,80,80, |
| 9898 | | pixel 3.32, shown at level 1.66, step 1, values float32 |
| 9899 | | |
| 9900 | | > hide #!49 models |
| 9901 | | |
| 9902 | | > select add #50 |
| 9903 | | |
| 9904 | | 2 models selected |
| 9905 | | |
| 9906 | | > ui mousemode right "rotate selected models" |
| 9907 | | |
| 9908 | | > view matrix models |
| 9909 | | > #50,0.25626,0.96633,0.023099,-33.544,0.9662,-0.25539,-0.035012,44.222,-0.027934,0.03129,-0.99912,256.23 |
| 9910 | | |
| 9911 | | > volume #50 level 2.579 |
| 9912 | | |
| 9913 | | > select subtract #50 |
| 9914 | | |
| 9915 | | Nothing selected |
| 9916 | | |
| 9917 | | > volume #50 level 1.975 |
| 9918 | | |
| 9919 | | > volume flip #50 |
| 9920 | | |
| 9921 | | Opened cryosparc_P4_J304_002_volume_map_sharp.mrc z flip as #51, grid size |
| 9922 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 9923 | | |
| 9924 | | > close #50 |
| 9925 | | |
| 9926 | | > rename #51 CS_P4_J304_sharp_flip id #50 |
| 9927 | | |
| 9928 | | > save CS_P4_J304_sharp_flip.mrc #50 |
| 9929 | | |
| 9930 | | > select add #50 |
| 9931 | | |
| 9932 | | 2 models selected |
| 9933 | | |
| 9934 | | > show #!49 models |
| 9935 | | |
| 9936 | | > view matrix models |
| 9937 | | > #50,0.25432,0.96619,0.042346,-35.759,0.96711,-0.25431,-0.0057645,40.158,0.0051995,0.042419,-0.99909,250.22 |
| 9938 | | |
| 9939 | | > view matrix models |
| 9940 | | > #50,-0.77557,0.61543,-0.14049,175.22,-0.61485,-0.78688,-0.052719,333.03,-0.143,0.045495,0.98868,11.993 |
| 9941 | | |
| 9942 | | > color #50 #FCEDFF |
| 9943 | | |
| 9944 | | > select subtract #50 |
| 9945 | | |
| 9946 | | Nothing selected |
| 9947 | | |
| 9948 | | > hide #!49 models |
| 9949 | | |
| 9950 | | > hide #!50 models |
| 9951 | | |
| 9952 | | > show #!50 models |
| 9953 | | |
| 9954 | | > volume #50 level 1.954 |
| 9955 | | |
| 9956 | | > open "/Users/peiyuyu/Documents/Goethe |
| 9957 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 9958 | | > F_B1/cryosparc_P4_J305_003_volume_map_sharp.mrc" |
| 9959 | | |
| 9960 | | Opened cryosparc_P4_J305_003_volume_map_sharp.mrc as #51, grid size 80,80,80, |
| 9961 | | pixel 3.32, shown at level 0.569, step 1, values float32 |
| 9962 | | |
| 9963 | | > hide #!50 models |
| 9964 | | |
| 9965 | | > select add #51 |
| 9966 | | |
| 9967 | | 2 models selected |
| 9968 | | |
| 9969 | | > view matrix models |
| 9970 | | > #51,-0.9391,-0.22026,0.26379,268.58,-0.1463,0.9508,0.27307,-8.1662,-0.31096,0.21785,-0.92512,264.01 |
| 9971 | | |
| 9972 | | > volume flip #51 |
| 9973 | | |
| 9974 | | Opened cryosparc_P4_J305_003_volume_map_sharp.mrc z flip as #52, grid size |
| 9975 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 9976 | | |
| 9977 | | > close #51 |
| 9978 | | |
| 9979 | | > rename #52 CS_P4_J305_sharp_flip id #51 |
| 9980 | | |
| 9981 | | > save CS_P4_J305_sharp_flip.mrc #51 |
| 9982 | | |
| 9983 | | > color #51 #FCEDFF |
| 9984 | | |
| 9985 | | > show #!50 models |
| 9986 | | |
| 9987 | | > select add #51 |
| 9988 | | |
| 9989 | | 2 models selected |
| 9990 | | |
| 9991 | | > view matrix models |
| 9992 | | > #51,0.99548,-0.078687,0.053201,5.068,0.073705,0.99322,0.089881,-20.578,-0.059913,-0.085553,0.99453,15.159 |
| 9993 | | |
| 9994 | | > fitmap #51 inMap #50 |
| 9995 | | |
| 9996 | | Fit map CS_P4_J305_sharp_flip.mrc in map CS_P4_J304_sharp_flip.mrc using 5112 |
| 9997 | | points |
| 9998 | | correlation = 0.7769, correlation about mean = 0.3462, overlap = 4325 |
| 9999 | | steps = 92, shift = 6.39, angle = 27.1 degrees |
| 10000 | | |
| 10001 | | Position of CS_P4_J305_sharp_flip.mrc (#51) relative to |
| 10002 | | CS_P4_J304_sharp_flip.mrc (#50) coordinates: |
| 10003 | | Matrix rotation and translation |
| 10004 | | -0.48191930 -0.86211205 -0.15657780 337.25915719 |
| 10005 | | 0.86174903 -0.49867166 0.09335524 70.84333299 |
| 10006 | | -0.15856359 -0.08994107 0.98324371 36.25215794 |
| 10007 | | Axis -0.10573285 0.00114549 0.99439391 |
| 10008 | | Axis point 149.95468753 133.49561797 0.00000000 |
| 10009 | | Rotation angle (degrees) 119.91228641 |
| 10010 | | Shift along axis 0.47070508 |
| 10011 | | |
| 10012 | | |
| 10013 | | > select subtract #51 |
| 10014 | | |
| 10015 | | Nothing selected |
| 10016 | | |
| 10017 | | > hide #!50 models |
| 10018 | | |
| 10019 | | > hide #!51 models |
| 10020 | | |
| 10021 | | > open "/Users/peiyuyu/Documents/Goethe |
| 10022 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10023 | | > F_B1/cryosparc_P4_J306_002_volume_map_sharp.mrc" |
| 10024 | | |
| 10025 | | Opened cryosparc_P4_J306_002_volume_map_sharp.mrc as #52, grid size 80,80,80, |
| 10026 | | pixel 3.32, shown at level 0.89, step 1, values float32 |
| 10027 | | |
| 10028 | | > select add #52 |
| 10029 | | |
| 10030 | | 2 models selected |
| 10031 | | |
| 10032 | | > view matrix models |
| 10033 | | > #52,0.64736,0.71151,-0.27328,-15.881,0.69766,-0.69754,-0.16345,155.65,-0.30692,-0.084848,-0.94795,287.9 |
| 10034 | | |
| 10035 | | > volume flip #52 |
| 10036 | | |
| 10037 | | Opened cryosparc_P4_J306_002_volume_map_sharp.mrc z flip as #53, grid size |
| 10038 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 10039 | | |
| 10040 | | > close #52 |
| 10041 | | |
| 10042 | | > select add #53 |
| 10043 | | |
| 10044 | | 2 models selected |
| 10045 | | |
| 10046 | | > show #!51 models |
| 10047 | | |
| 10048 | | > view matrix models |
| 10049 | | > #53,-0.54969,0.82414,-0.13652,114.28,-0.83504,-0.54665,0.062273,314.18,-0.023306,0.14823,0.98868,-59.023 |
| 10050 | | |
| 10051 | | > ui mousemode right "translate selected models" |
| 10052 | | |
| 10053 | | > view matrix models |
| 10054 | | > #53,-0.54969,0.82414,-0.13652,112.71,-0.83504,-0.54665,0.062273,313.11,-0.023306,0.14823,0.98868,-22.329 |
| 10055 | | |
| 10056 | | > fitmap #53 inMap #51 |
| 10057 | | |
| 10058 | | Fit map cryosparc_P4_J306_002_volume_map_sharp.mrc z flip in map |
| 10059 | | CS_P4_J305_sharp_flip.mrc using 5118 points |
| 10060 | | correlation = 0.8696, correlation about mean = 0.2872, overlap = 4918 |
| 10061 | | steps = 72, shift = 6.47, angle = 20.6 degrees |
| 10062 | | |
| 10063 | | Position of cryosparc_P4_J306_002_volume_map_sharp.mrc z flip (#53) relative |
| 10064 | | to CS_P4_J305_sharp_flip.mrc (#51) coordinates: |
| 10065 | | Matrix rotation and translation |
| 10066 | | -0.48951817 0.85648171 -0.16374082 109.67271887 |
| 10067 | | -0.85572951 -0.50794021 -0.09860917 331.28158536 |
| 10068 | | -0.16762749 0.09184687 0.98156263 12.77250071 |
| 10069 | | Axis 0.11055185 0.00225605 -0.99386780 |
| 10070 | | Axis point 150.74970822 134.14339059 0.00000000 |
| 10071 | | Rotation angle (degrees) 120.52723515 |
| 10072 | | Shift along axis 0.17773418 |
| 10073 | | |
| 10074 | | |
| 10075 | | > select subtract #53 |
| 10076 | | |
| 10077 | | Nothing selected |
| 10078 | | |
| 10079 | | > hide #!51 models |
| 10080 | | |
| 10081 | | > rename #53 CS_P4_J306_sharp_flip id #52 |
| 10082 | | |
| 10083 | | > save CS_P4_J306_sharp_flip.mrc #52 |
| 10084 | | |
| 10085 | | > open "/Users/peiyuyu/Documents/Goethe |
| 10086 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10087 | | > F_B1/cryosparc_P4_J307_002_volume_map_sharp.mrc" |
| 10088 | | |
| 10089 | | Opened cryosparc_P4_J307_002_volume_map_sharp.mrc as #53, grid size 80,80,80, |
| 10090 | | pixel 3.32, shown at level 0.417, step 1, values float32 |
| 10091 | | |
| 10092 | | > hide #!52 models |
| 10093 | | |
| 10094 | | > select add #53 |
| 10095 | | |
| 10096 | | 2 models selected |
| 10097 | | |
| 10098 | | > ui mousemode right "rotate selected models" |
| 10099 | | |
| 10100 | | > view matrix models |
| 10101 | | > #53,-0.97802,-0.15767,0.13647,275.15,-0.15325,0.98728,0.042392,17.478,-0.14141,0.020547,-0.98974,260.92 |
| 10102 | | |
| 10103 | | > volume flip #53 |
| 10104 | | |
| 10105 | | Opened cryosparc_P4_J307_002_volume_map_sharp.mrc z flip as #54, grid size |
| 10106 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 10107 | | |
| 10108 | | > close #53 |
| 10109 | | |
| 10110 | | > select add #54 |
| 10111 | | |
| 10112 | | 2 models selected |
| 10113 | | |
| 10114 | | > show #!51 models |
| 10115 | | |
| 10116 | | > view matrix models |
| 10117 | | > #54,-0.83454,0.54308,0.09272,165.84,-0.53719,-0.83947,0.081916,314.21,0.12232,0.018554,0.99232,-51.868 |
| 10118 | | |
| 10119 | | > ui mousemode right "translate selected models" |
| 10120 | | |
| 10121 | | > view matrix models |
| 10122 | | > #54,-0.83454,0.54308,0.09272,166.01,-0.53719,-0.83947,0.081916,312.6,0.12232,0.018554,0.99232,-21.112 |
| 10123 | | |
| 10124 | | > fitmap #54 inMap #51 |
| 10125 | | |
| 10126 | | Fit map cryosparc_P4_J307_002_volume_map_sharp.mrc z flip in map |
| 10127 | | CS_P4_J305_sharp_flip.mrc using 5118 points |
| 10128 | | correlation = 0.9157, correlation about mean = 0.3086, overlap = 1346 |
| 10129 | | steps = 92, shift = 8.03, angle = 16.7 degrees |
| 10130 | | |
| 10131 | | Position of cryosparc_P4_J307_002_volume_map_sharp.mrc z flip (#54) relative |
| 10132 | | to CS_P4_J305_sharp_flip.mrc (#51) coordinates: |
| 10133 | | Matrix rotation and translation |
| 10134 | | -0.49196091 0.85478647 -0.16527113 110.59805296 |
| 10135 | | -0.85414546 -0.51062861 -0.09845785 330.98838770 |
| 10136 | | -0.16855261 0.09272817 0.98132130 13.11747971 |
| 10137 | | Axis 0.11118076 0.00190829 -0.99379837 |
| 10138 | | Axis point 150.81392912 133.83273648 0.00000000 |
| 10139 | | Rotation angle (degrees) 120.70607669 |
| 10140 | | Shift along axis -0.10813355 |
| 10141 | | |
| 10142 | | |
| 10143 | | > select subtract #54 |
| 10144 | | |
| 10145 | | Nothing selected |
| 10146 | | |
| 10147 | | > rename #54 CS_P4_J307_sharp_flip id #53 |
| 10148 | | |
| 10149 | | > save CS_P4_J307_sharp_flip.mrc #53 |
| 10150 | | |
| 10151 | | > open "/Users/peiyuyu/Documents/Goethe |
| 10152 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10153 | | > F_B1/cryosparc_P4_J308_003_volume_map_sharp.mrc" |
| 10154 | | |
| 10155 | | Opened cryosparc_P4_J308_003_volume_map_sharp.mrc as #54, grid size 80,80,80, |
| 10156 | | pixel 3.32, shown at level 0.487, step 1, values float32 |
| 10157 | | |
| 10158 | | > hide #!51 models |
| 10159 | | |
| 10160 | | > hide #!53 models |
| 10161 | | |
| 10162 | | > select add #54 |
| 10163 | | |
| 10164 | | 2 models selected |
| 10165 | | |
| 10166 | | > view matrix models #54,1,0,0,-2.2085,0,1,0,5.106,0,0,1,17.329 |
| 10167 | | |
| 10168 | | > ui mousemode right "rotate selected models" |
| 10169 | | |
| 10170 | | > view matrix models |
| 10171 | | > #54,-0.97907,-0.098429,0.17813,263.99,-0.078474,0.99017,0.11581,3.2524,-0.18778,0.099408,-0.97717,270.12 |
| 10172 | | |
| 10173 | | > volume flip #54 |
| 10174 | | |
| 10175 | | Opened cryosparc_P4_J308_003_volume_map_sharp.mrc z flip as #55, grid size |
| 10176 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 10177 | | |
| 10178 | | > close #54 |
| 10179 | | |
| 10180 | | > select add #55 |
| 10181 | | |
| 10182 | | 2 models selected |
| 10183 | | |
| 10184 | | > show #!53 models |
| 10185 | | |
| 10186 | | > view matrix models |
| 10187 | | > #55,-0.032009,-0.99944,0.0094019,279.03,0.99475,-0.030941,0.097574,-4.0943,-0.097229,0.012476,0.99518,-8.8511 |
| 10188 | | |
| 10189 | | > ui mousemode right "translate selected models" |
| 10190 | | |
| 10191 | | > view matrix models |
| 10192 | | > #55,-0.032009,-0.99944,0.0094019,275.5,0.99475,-0.030941,0.097574,4.3503,-0.097229,0.012476,0.99518,10.879 |
| 10193 | | |
| 10194 | | > fitmap #55 inMap #53 |
| 10195 | | |
| 10196 | | Fit map cryosparc_P4_J308_003_volume_map_sharp.mrc z flip in map |
| 10197 | | CS_P4_J307_sharp_flip.mrc using 5116 points |
| 10198 | | correlation = 0.9635, correlation about mean = 0.3444, overlap = 1309 |
| 10199 | | steps = 80, shift = 11, angle = 8.02 degrees |
| 10200 | | |
| 10201 | | Position of cryosparc_P4_J308_003_volume_map_sharp.mrc z flip (#55) relative |
| 10202 | | to CS_P4_J307_sharp_flip.mrc (#53) coordinates: |
| 10203 | | Matrix rotation and translation |
| 10204 | | -0.47079667 0.87008681 -0.14594324 102.09450866 |
| 10205 | | -0.87101131 -0.48471046 -0.07996914 326.37268834 |
| 10206 | | -0.14032031 0.08946901 0.98605553 9.07494900 |
| 10207 | | Axis 0.09685875 -0.00321433 -0.99529295 |
| 10208 | | Axis point 148.07793015 132.95152312 0.00000000 |
| 10209 | | Rotation angle (degrees) 118.99450917 |
| 10210 | | Shift along axis -0.19255539 |
| 10211 | | |
| 10212 | | |
| 10213 | | > select subtract #55 |
| 10214 | | |
| 10215 | | Nothing selected |
| 10216 | | |
| 10217 | | > hide #!53 models |
| 10218 | | |
| 10219 | | > color #55 #fcedffff models |
| 10220 | | |
| 10221 | | > rename #55 CS_P4_J308_sharp_flip id #54 |
| 10222 | | |
| 10223 | | > save CS_P4_J308_sharp_flip.mrc #54 |
| 10224 | | |
| 10225 | | > open "/Users/peiyuyu/Documents/Goethe |
| 10226 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10227 | | > F_B1/cryosparc_P4_J309_002_volume_map_sharp.mrc" |
| 10228 | | |
| 10229 | | Opened cryosparc_P4_J309_002_volume_map_sharp.mrc as #55, grid size 80,80,80, |
| 10230 | | pixel 3.32, shown at level 0.427, step 1, values float32 |
| 10231 | | |
| 10232 | | > hide #!54 models |
| 10233 | | |
| 10234 | | > select add #55 |
| 10235 | | |
| 10236 | | 2 models selected |
| 10237 | | |
| 10238 | | > show #!54 models |
| 10239 | | |
| 10240 | | > ui mousemode right "rotate selected models" |
| 10241 | | |
| 10242 | | > view matrix models |
| 10243 | | > #55,0.72482,-0.68817,-0.032661,133.9,-0.68882,-0.72298,-0.053201,331.74,0.012998,0.061058,-0.99805,234.36 |
| 10244 | | |
| 10245 | | > hide #!54 models |
| 10246 | | |
| 10247 | | > view matrix models |
| 10248 | | > #55,-0.98503,0.14104,0.099091,242.98,0.14443,0.98912,0.027904,-21.89,-0.094078,0.041798,-0.99469,251.29 |
| 10249 | | |
| 10250 | | > volume flip #55 |
| 10251 | | |
| 10252 | | Opened cryosparc_P4_J309_002_volume_map_sharp.mrc z flip as #56, grid size |
| 10253 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 10254 | | |
| 10255 | | > close #55 |
| 10256 | | |
| 10257 | | > select add #56 |
| 10258 | | |
| 10259 | | 2 models selected |
| 10260 | | |
| 10261 | | > show #!54 models |
| 10262 | | |
| 10263 | | > view matrix models |
| 10264 | | > #56,-0.63923,0.76709,0.054278,117.91,-0.76391,-0.64153,0.069883,315.42,0.088428,0.0032076,0.99608,-48.086 |
| 10265 | | |
| 10266 | | > ui mousemode right "translate selected models" |
| 10267 | | |
| 10268 | | > view matrix models |
| 10269 | | > #56,-0.63923,0.76709,0.054278,118.17,-0.76391,-0.64153,0.069883,313.6,0.088428,0.0032076,0.99608,-18.356 |
| 10270 | | |
| 10271 | | > fitmap #56 inMap #54 |
| 10272 | | |
| 10273 | | Fit map cryosparc_P4_J309_002_volume_map_sharp.mrc z flip in map |
| 10274 | | CS_P4_J308_sharp_flip.mrc using 5111 points |
| 10275 | | correlation = 0.9585, correlation about mean = 0.3571, overlap = 1283 |
| 10276 | | steps = 104, shift = 10.8, angle = 17.1 degrees |
| 10277 | | |
| 10278 | | Position of cryosparc_P4_J309_002_volume_map_sharp.mrc z flip (#56) relative |
| 10279 | | to CS_P4_J308_sharp_flip.mrc (#54) coordinates: |
| 10280 | | Matrix rotation and translation |
| 10281 | | -0.48831434 -0.86312376 -0.12871093 333.56502617 |
| 10282 | | 0.86459679 -0.49852007 0.06285020 75.43057430 |
| 10283 | | -0.11841248 -0.08059241 0.98968852 28.18171624 |
| 10284 | | Axis -0.08273806 -0.00594017 0.99655362 |
| 10285 | | Axis point 146.06051902 134.60897835 0.00000000 |
| 10286 | | Rotation angle (degrees) 119.90563147 |
| 10287 | | Shift along axis 0.03799683 |
| 10288 | | |
| 10289 | | |
| 10290 | | > select subtract #56 |
| 10291 | | |
| 10292 | | Nothing selected |
| 10293 | | |
| 10294 | | > hide #!54 models |
| 10295 | | |
| 10296 | | > hide #!56 models |
| 10297 | | |
| 10298 | | > show #!56 models |
| 10299 | | |
| 10300 | | > color #56 #fcedffff models |
| 10301 | | |
| 10302 | | > rename #56 CS_P4_J309_sharp_flip id #55 |
| 10303 | | |
| 10304 | | > save CS_P4_J309_sharp_flip.mrc #55 |
| 10305 | | |
| 10306 | | > open "/Users/peiyuyu/Documents/Goethe |
| 10307 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10308 | | > F_B1/cryosparc_P4_J310_003_volume_map_sharp.mrc" |
| 10309 | | |
| 10310 | | Opened cryosparc_P4_J310_003_volume_map_sharp.mrc as #56, grid size 80,80,80, |
| 10311 | | pixel 3.32, shown at level 0.683, step 1, values float32 |
| 10312 | | |
| 10313 | | > volume flip #56 |
| 10314 | | |
| 10315 | | Opened cryosparc_P4_J310_003_volume_map_sharp.mrc z flip as #57, grid size |
| 10316 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 10317 | | |
| 10318 | | > close #56 |
| 10319 | | |
| 10320 | | > fitmap #56 inMap #52 |
| 10321 | | |
| 10322 | | No atoms or maps for #56 |
| 10323 | | |
| 10324 | | > fitmap #57 inMap #52 |
| 10325 | | |
| 10326 | | Fit map cryosparc_P4_J310_003_volume_map_sharp.mrc z flip in map |
| 10327 | | CS_P4_J306_sharp_flip.mrc using 5115 points |
| 10328 | | correlation = 0.769, correlation about mean = 0.3701, overlap = 4288 |
| 10329 | | steps = 88, shift = 5.42, angle = 22.7 degrees |
| 10330 | | |
| 10331 | | Position of cryosparc_P4_J310_003_volume_map_sharp.mrc z flip (#57) relative |
| 10332 | | to CS_P4_J306_sharp_flip.mrc (#52) coordinates: |
| 10333 | | Matrix rotation and translation |
| 10334 | | -0.48166864 -0.85899522 -0.17355843 339.66736714 |
| 10335 | | 0.86194402 -0.50013127 0.08319372 72.72621082 |
| 10336 | | -0.15826501 -0.10952585 0.98130335 38.95738038 |
| 10337 | | Axis -0.11128512 -0.00883113 0.99374928 |
| 10338 | | Axis point 150.89500052 135.18260516 0.00000000 |
| 10339 | | Rotation angle (degrees) 120.01642768 |
| 10340 | | Shift along axis 0.27169032 |
| 10341 | | |
| 10342 | | |
| 10343 | | > hide #!55 models |
| 10344 | | |
| 10345 | | > show #!52 models |
| 10346 | | |
| 10347 | | > color #57 #fcedffff models |
| 10348 | | |
| 10349 | | > hide #!52 models |
| 10350 | | |
| 10351 | | > save #57 CS_P4_J310_sharp_flip id #56 |
| 10352 | | |
| 10353 | | Cannot determine format for '#57' |
| 10354 | | |
| 10355 | | > rename #57 CS_P4_J310_sharp_flip id #56 |
| 10356 | | |
| 10357 | | > save CS_P4_J310_sharp_flip.mrc #56 |
| 10358 | | |
| 10359 | | > open "/Users/peiyuyu/Documents/Goethe |
| 10360 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10361 | | > F_B1/cryosparc_P4_J311_004_volume_map_sharp.mrc" |
| 10362 | | |
| 10363 | | Opened cryosparc_P4_J311_004_volume_map_sharp.mrc as #57, grid size 80,80,80, |
| 10364 | | pixel 3.32, shown at level 0.525, step 1, values float32 |
| 10365 | | |
| 10366 | | > volume flip #57 |
| 10367 | | |
| 10368 | | Opened cryosparc_P4_J311_004_volume_map_sharp.mrc z flip as #58, grid size |
| 10369 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 10370 | | |
| 10371 | | > close #57 |
| 10372 | | |
| 10373 | | > color #58 #fcedffff models |
| 10374 | | |
| 10375 | | > rename #58 CS_P4_J311_sharp_flip id #57 |
| 10376 | | |
| 10377 | | > save CS_P4_J311_sharp_flip.mrc #57 |
| 10378 | | |
| 10379 | | > save "/Users/peiyuyu/Documents/Goethe |
| 10380 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10381 | | > F_B1/20260506_SA-F_B1_workflow.cxs" |
| 10382 | | |
| 10383 | | > view P1 |
| 10384 | | |
| 10385 | | > hide #!57 models |
| 10386 | | |
| 10387 | | > hide #!56 models |
| 10388 | | |
| 10389 | | > show #!47 models |
| 10390 | | |
| 10391 | | > hide #!47 models |
| 10392 | | |
| 10393 | | > show #!48 models |
| 10394 | | |
| 10395 | | > save CS_P4_J302_NU_sharp.png supersample 3 transparentBackground true |
| 10396 | | |
| 10397 | | > hide #!48 models |
| 10398 | | |
| 10399 | | > show #!49 models |
| 10400 | | |
| 10401 | | > save CS_P4_J303_NU_sharp.png supersample 3 transparentBackground true |
| 10402 | | |
| 10403 | | > hide #!49 models |
| 10404 | | |
| 10405 | | > show #!50 models |
| 10406 | | |
| 10407 | | > save CS_P4_J304_NU_sharp.png supersample 3 transparentBackground true |
| 10408 | | |
| 10409 | | > hide #!50 models |
| 10410 | | |
| 10411 | | > show #!51 models |
| 10412 | | |
| 10413 | | > save CS_P4_J305_NU_sharp.png supersample 3 transparentBackground true |
| 10414 | | |
| 10415 | | > hide #!51 models |
| 10416 | | |
| 10417 | | > show #!52 models |
| 10418 | | |
| 10419 | | > volume #52 level 1.034 |
| 10420 | | |
| 10421 | | > save CS_P4_J306_NU_sharp.png supersample 3 transparentBackground true |
| 10422 | | |
| 10423 | | > hide #!52 models |
| 10424 | | |
| 10425 | | > show #!53 models |
| 10426 | | |
| 10427 | | > color #53 #fcedffff models |
| 10428 | | |
| 10429 | | > save CS_P4_J307_NU_sharp.png supersample 3 transparentBackground true |
| 10430 | | |
| 10431 | | > hide #!53 models |
| 10432 | | |
| 10433 | | > show #!54 models |
| 10434 | | |
| 10435 | | > save CS_P4_J308_NU_sharp.png supersample 3 transparentBackground true |
| 10436 | | |
| 10437 | | > hide #!54 models |
| 10438 | | |
| 10439 | | > show #!55 models |
| 10440 | | |
| 10441 | | > save CS_P4_J309_NU_sharp.png supersample 3 transparentBackground true |
| 10442 | | |
| 10443 | | > hide #!55 models |
| 10444 | | |
| 10445 | | > show #!56 models |
| 10446 | | |
| 10447 | | > volume #56 level 0.7192 |
| 10448 | | |
| 10449 | | > save CS_P4_J310_NU_sharp.png supersample 3 transparentBackground true |
| 10450 | | |
| 10451 | | > hide #!56 models |
| 10452 | | |
| 10453 | | > show #!57 models |
| 10454 | | |
| 10455 | | > save CS_P4_J311_NU_sharp.png supersample 3 transparentBackground true |
| 10456 | | |
| 10457 | | > save "/Users/peiyuyu/Documents/Goethe |
| 10458 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10459 | | > F_B1/20260506_SA-F_B1_workflow.cxs" |
| 10460 | | |
| 10461 | | ——— End of log from Wed May 6 15:19:28 2026 ——— |
| 10462 | | |
| 10463 | | > view name session-start |
| 10464 | | |
| 10465 | | opened ChimeraX session |
| 10466 | | |
| 10467 | | > open "/Users/peiyuyu/Documents/Goethe |
| 10468 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10469 | | > F_B1/cryosparc_P4_J323_006_volume_map_sharp.mrc" |
| 10470 | | |
| 10471 | | Opened cryosparc_P4_J323_006_volume_map_sharp.mrc as #58, grid size |
| 10472 | | 320,320,320, pixel 0.831, shown at level 0.0345, step 2, values float32 |
| 10473 | | |
| 10474 | | > open "/Users/peiyuyu/Documents/Goethe |
| 10475 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10476 | | > F_B1/cryosparc_P4_J324_map_locres.mrc" |
| 10477 | | |
| 10478 | | Opened cryosparc_P4_J324_map_locres.mrc as #59, grid size 320,320,320, pixel |
| 10479 | | 0.831, shown at level 8.89, step 2, values float32 |
| 10480 | | |
| 10481 | | > hide #!57 models |
| 10482 | | |
| 10483 | | > hide #!58 models |
| 10484 | | |
| 10485 | | > show #!58 models |
| 10486 | | |
| 10487 | | > hide #!59 models |
| 10488 | | |
| 10489 | | > show #!59 models |
| 10490 | | |
| 10491 | | > volume flip #58 |
| 10492 | | |
| 10493 | | Opened cryosparc_P4_J323_006_volume_map_sharp.mrc z flip as #60, grid size |
| 10494 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 10495 | | |
| 10496 | | > volume flip #59 |
| 10497 | | |
| 10498 | | Opened cryosparc_P4_J324_map_locres.mrc z flip as #61, grid size 320,320,320, |
| 10499 | | pixel 0.831, shown at step 1, values float32 |
| 10500 | | |
| 10501 | | > close #58 |
| 10502 | | |
| 10503 | | > close #59 |
| 10504 | | |
| 10505 | | > rename #60 CS_P4_J323_sharp_flip #58 |
| 10506 | | |
| 10507 | | Expected a keyword |
| 10508 | | |
| 10509 | | > rename #60 CS_P4_J323_sharp_flip id #58 |
| 10510 | | |
| 10511 | | > save CS_P4_J323_sharp_flip.mrc #58 |
| 10512 | | |
| 10513 | | > rename CS_P4_J324_map_locres_flip id #59 |
| 10514 | | |
| 10515 | | Missing or invalid "models" argument: invalid models specifier |
| 10516 | | |
| 10517 | | > rename #61 CS_P4_J324_map_locres_flip id #59 |
| 10518 | | |
| 10519 | | > save CS_P4_J324_map_locres_flip.mrc #59 |
| 10520 | | |
| 10521 | | > hide #!58 models |
| 10522 | | |
| 10523 | | > show #!58 models |
| 10524 | | |
| 10525 | | > hide #!59 models |
| 10526 | | |
| 10527 | | > volume #59 level 9.377 |
| 10528 | | |
| 10529 | | > hide #!59 models |
| 10530 | | |
| 10531 | | > volume #58 level 0.05889 |
| 10532 | | |
| 10533 | | > show #!59 models |
| 10534 | | |
| 10535 | | > volume #59 level 3.695 |
| 10536 | | |
| 10537 | | > save "/Users/peiyuyu/Documents/Goethe |
| 10538 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10539 | | > F_B1/20260507_SA-F_B1_workflow.cxs" |
| 10540 | | |
| 10541 | | > ui tool show "Surface Color" |
| 10542 | | |
| 10543 | | > hide #!58 models |
| 10544 | | |
| 10545 | | > show #!58 models |
| 10546 | | |
| 10547 | | > hide #!59 models |
| 10548 | | |
| 10549 | | > color radial #58.1 palette #ffb17f:#4979d3:#99bfe5:#fff6c0:#f4fff3 |
| 10550 | | |
| 10551 | | > color sample #58.1 map #59 palette |
| 10552 | | > 166.9,#ffb17f:198,#4979d3:229.1,#99bfe5:260.3,#fff6c0:291.4,#f4fff3 |
| 10553 | | > outsideColor #808080 |
| 10554 | | |
| 10555 | | Map values for surface "surface": minimum 2.944, mean 3.552, maximum 44.59 |
| 10556 | | |
| 10557 | | > color sample #58.1 map #59 palette |
| 10558 | | > 2,#ffb17f:3,#4979d3:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 10559 | | |
| 10560 | | Map values for surface "surface": minimum 2.944, mean 3.552, maximum 44.59 |
| 10561 | | |
| 10562 | | > show #!59 models |
| 10563 | | |
| 10564 | | > hide #!58 models |
| 10565 | | |
| 10566 | | > hide #!59 models |
| 10567 | | |
| 10568 | | > show #!59 models |
| 10569 | | |
| 10570 | | > show #!58 models |
| 10571 | | |
| 10572 | | > hide #!59 models |
| 10573 | | |
| 10574 | | > show #!59 models |
| 10575 | | |
| 10576 | | > hide #!59 models |
| 10577 | | |
| 10578 | | > volume #58 level 0.0634 |
| 10579 | | |
| 10580 | | > volume #58 level 0.04264 |
| 10581 | | |
| 10582 | | > volume #58 level 0.07693 |
| 10583 | | |
| 10584 | | > volume #58 level 0.06069 |
| 10585 | | |
| 10586 | | > volume #58 level 0.07152 |
| 10587 | | |
| 10588 | | > volume #58 level 0.05889 |
| 10589 | | |
| 10590 | | > volume #58 level 0.05167 |
| 10591 | | |
| 10592 | | > volume #58 level 0.06159 |
| 10593 | | |
| 10594 | | > key #ffb17f:2 #4979d3:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 10595 | | |
| 10596 | | > ui mousemode right "color key" |
| 10597 | | |
| 10598 | | > key pos 0.82265,0.196044 size 0.0692066,0.202924 |
| 10599 | | |
| 10600 | | > ui mousemode right translate |
| 10601 | | |
| 10602 | | > ui mousemode right "color key" |
| 10603 | | |
| 10604 | | > key pos 0.802943,0.231339 size 0.039822,0.144632 |
| 10605 | | |
| 10606 | | > key #ffb17f:2 #4979d3:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 10607 | | |
| 10608 | | > key pos 0.836801,0.165731 size 0.0516869,0.238605 |
| 10609 | | |
| 10610 | | > show #41 models |
| 10611 | | |
| 10612 | | > key pos 0.820538,0.190547 size 0.0458442,0.242723 |
| 10613 | | |
| 10614 | | > save CS_P4_J323_sharp_flip.png supersample 3 transparentBackground true |
| 10615 | | |
| 10616 | | > save "/Users/peiyuyu/Documents/Goethe |
| 10617 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10618 | | > F_B1/20260507_SA-F_B1_workflow1.cxs" |
| 10619 | | |
| 10620 | | > color sample #58.1 map #59 palette |
| 10621 | | > 2,#ffb17f:3,#169bd3:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 10622 | | |
| 10623 | | Map values for surface "surface": minimum 2.944, mean 3.532, maximum 44.61 |
| 10624 | | |
| 10625 | | > color sample #58.1 map #59 palette |
| 10626 | | > 2,#ffb17f:3,#46c7f6:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 10627 | | |
| 10628 | | Map values for surface "surface": minimum 2.944, mean 3.532, maximum 44.61 |
| 10629 | | |
| 10630 | | > color sample #58.1 map #59 palette |
| 10631 | | > 2,#ffb17f:3,#11b6e1:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 10632 | | |
| 10633 | | Map values for surface "surface": minimum 2.944, mean 3.532, maximum 44.61 |
| 10634 | | |
| 10635 | | > key #ffb17f:2 #11b6e1:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 10636 | | |
| 10637 | | > ui mousemode right "color key" |
| 10638 | | |
| 10639 | | > color sample #58.1 map #59 palette |
| 10640 | | > 2,#ffb17f:3,#11b6e1:4,#99bfe5:5,#fff6c0:6,#ffb17f outsideColor #808080 |
| 10641 | | |
| 10642 | | Map values for surface "surface": minimum 2.944, mean 3.532, maximum 44.61 |
| 10643 | | |
| 10644 | | > key #ffb17f:2 #11b6e1:3 #99bfe5:4 #fff6c0:5 #ffb17f:6 showTool true |
| 10645 | | |
| 10646 | | > color sample #58.1 map #59 palette |
| 10647 | | > 2,#13097f:3,#11b6e1:4,#99bfe5:5,#fff6c0:6,#ffb17f outsideColor #808080 |
| 10648 | | |
| 10649 | | Map values for surface "surface": minimum 2.944, mean 3.532, maximum 44.61 |
| 10650 | | |
| 10651 | | > key #13097f:2 #11b6e1:3 #99bfe5:4 #fff6c0:5 #ffb17f:6 showTool true |
| 10652 | | |
| 10653 | | > save CS_P4_J323_sharp_flip2.png supersample 3 transparentBackground true |
| 10654 | | |
| 10655 | | > save "/Users/peiyuyu/Documents/Goethe |
| 10656 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10657 | | > F_B1/20260507_SA-F_B1_workflow2.cxs" |
| 10658 | | |
| 10659 | | > open "/Users/peiyuyu/Documents/Goethe |
| 10660 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10661 | | > F_B1/cryosparc_P4_J329_005_volume_map_sharp.mrc" |
| 10662 | | |
| 10663 | | Opened cryosparc_P4_J329_005_volume_map_sharp.mrc as #60, grid size |
| 10664 | | 320,320,320, pixel 0.831, shown at level 0.0384, step 2, values float32 |
| 10665 | | |
| 10666 | | > open "/Users/peiyuyu/Documents/Goethe |
| 10667 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10668 | | > F_B1/cryosparc_P4_J330_map_locres.mrc" |
| 10669 | | |
| 10670 | | Opened cryosparc_P4_J330_map_locres.mrc as #61, grid size 320,320,320, pixel |
| 10671 | | 0.831, shown at level 9.71, step 2, values float32 |
| 10672 | | |
| 10673 | | > hide #!58 models |
| 10674 | | |
| 10675 | | > hide #!60 models |
| 10676 | | |
| 10677 | | > show #!60 models |
| 10678 | | |
| 10679 | | > hide #!61 models |
| 10680 | | |
| 10681 | | > volume flip #60 |
| 10682 | | |
| 10683 | | Opened cryosparc_P4_J329_005_volume_map_sharp.mrc z flip as #62, grid size |
| 10684 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 10685 | | |
| 10686 | | > volume flip #61 |
| 10687 | | |
| 10688 | | Opened cryosparc_P4_J330_map_locres.mrc z flip as #63, grid size 320,320,320, |
| 10689 | | pixel 0.831, shown at step 1, values float32 |
| 10690 | | |
| 10691 | | > close #60 |
| 10692 | | |
| 10693 | | > close #61 |
| 10694 | | |
| 10695 | | > rename #62 CS_P4_J329_sharp_flip id #60 |
| 10696 | | |
| 10697 | | > save CS_P4_J329_sharp_flip.mrc #60 |
| 10698 | | |
| 10699 | | > rename #63 rename |
| 10700 | | |
| 10701 | | > rename #63 CS_P4_J330_locres_flip id #61 |
| 10702 | | |
| 10703 | | > save CS_P4_J330_locres_flip.mrc #61 |
| 10704 | | |
| 10705 | | > save "/Users/peiyuyu/Documents/Goethe |
| 10706 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10707 | | > F_B1/20260507_SA-F_B1_workflow3.cxs" |
| 10708 | | |
| 10709 | | > hide #!61 models |
| 10710 | | |
| 10711 | | > color sample #58.1 map #59 palette |
| 10712 | | > 2,#13097f:3,#11b6e1:4,#99bfe5:5,#fff6c0:6,#ffb17f outsideColor #808080 |
| 10713 | | |
| 10714 | | Map values for surface "surface": minimum 2.944, mean 3.532, maximum 44.61 |
| 10715 | | |
| 10716 | | > color sample #60.1 map #61 palette |
| 10717 | | > 2,#13097f:3,#11b6e1:4,#99bfe5:5,#fff6c0:6,#ffb17f outsideColor #808080 |
| 10718 | | |
| 10719 | | Map values for surface "surface": minimum 0, mean 4.466, maximum 46.71 |
| 10720 | | |
| 10721 | | > volume #60 level 0.06443 |
| 10722 | | |
| 10723 | | > key #13097f:2 #11b6e1:3 #99bfe5:4 #fff6c0:5 #ffb17f:6 showTool true |
| 10724 | | |
| 10725 | | > ui mousemode right "color key" |
| 10726 | | |
| 10727 | | > volume #60 level 0.05663 |
| 10728 | | |
| 10729 | | > save CS_P4_J329_locres_sharp.png supersample 3 transparentBackground true |
| 10730 | | |
| 10731 | | > save "/Users/peiyuyu/Documents/Goethe |
| 10732 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10733 | | > F_B1/20260507_SA-F_B1_workflow3.cxs" |
| 10734 | | |
| 10735 | | ——— End of log from Thu May 7 15:58:17 2026 ——— |
| 10736 | | |
| 10737 | | > view name session-start |
| 10738 | | |
| 10739 | | opened ChimeraX session |
| 10740 | | |
| 10741 | | > open "/Users/peiyuyu/Documents/Goethe |
| 10742 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10743 | | > F_B1/cryosparc_P4_J300_005_volume_map_sharp.mrc" |
| 10744 | | |
| 10745 | | Opened cryosparc_P4_J300_005_volume_map_sharp.mrc as #62, grid size |
| 10746 | | 320,320,320, pixel 0.831, shown at level 0.0375, step 2, values float32 |
| 10747 | | |
| 10748 | | > open "/Users/peiyuyu/Documents/Goethe |
| 10749 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10750 | | > F_B1/cryosparc_P4_J301_map_locres.mrc" |
| 10751 | | |
| 10752 | | Opened cryosparc_P4_J301_map_locres.mrc as #63, grid size 320,320,320, pixel |
| 10753 | | 0.831, shown at level 9.68, step 2, values float32 |
| 10754 | | |
| 10755 | | > hide #!60 models |
| 10756 | | |
| 10757 | | > hide #!63 models |
| 10758 | | |
| 10759 | | > volume flip #62 |
| 10760 | | |
| 10761 | | Opened cryosparc_P4_J300_005_volume_map_sharp.mrc z flip as #64, grid size |
| 10762 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 10763 | | |
| 10764 | | > volume flip #63 |
| 10765 | | |
| 10766 | | Opened cryosparc_P4_J301_map_locres.mrc z flip as #65, grid size 320,320,320, |
| 10767 | | pixel 0.831, shown at step 1, values float32 |
| 10768 | | |
| 10769 | | > close #62 |
| 10770 | | |
| 10771 | | > close #63 |
| 10772 | | |
| 10773 | | > rename #64 CS_P4_J300_sharp_flip id #62 |
| 10774 | | |
| 10775 | | > save CS_P4_J300_sharp_flip.mrc #62 |
| 10776 | | |
| 10777 | | > rename #65 CS_P4_J301_locres_flip id #63 |
| 10778 | | |
| 10779 | | > save CS_P4_J301_locres_flip.mrc #63 |
| 10780 | | |
| 10781 | | > show #!46 models |
| 10782 | | |
| 10783 | | > show #!47 models |
| 10784 | | |
| 10785 | | > hide #!47 models |
| 10786 | | |
| 10787 | | > hide #!46 models |
| 10788 | | |
| 10789 | | > hide #!63 models |
| 10790 | | |
| 10791 | | > show #!63 models |
| 10792 | | |
| 10793 | | > volume #63 level 3.952 |
| 10794 | | |
| 10795 | | > hide #!63 models |
| 10796 | | |
| 10797 | | > volume #62 level 0.0392 |
| 10798 | | |
| 10799 | | > ui tool show "Surface Color" |
| 10800 | | |
| 10801 | | > color sample #62.1 map #63 palette #13097f:#11b6e1:#99bfe5:#fff6c0:#ffb17f |
| 10802 | | > outsideColor #808080 |
| 10803 | | |
| 10804 | | Map values for surface "surface": minimum 0, mean 4.383, maximum 43.37 |
| 10805 | | |
| 10806 | | > color sample #62.1 map #63 palette |
| 10807 | | > 2,#13097f:3,#11b6e1:4,#99bfe5:5,#fff6c0:6,#ffb17f outsideColor #808080 |
| 10808 | | |
| 10809 | | Map values for surface "surface": minimum 0, mean 4.383, maximum 43.37 |
| 10810 | | |
| 10811 | | > show #!63 models |
| 10812 | | |
| 10813 | | > hide #!63 models |
| 10814 | | |
| 10815 | | > hide #!62 models |
| 10816 | | |
| 10817 | | > show #!62 models |
| 10818 | | |
| 10819 | | > volume #62 level 0.06614 |
| 10820 | | |
| 10821 | | > volume #62 level 0.05745 |
| 10822 | | |
| 10823 | | > save CS_P4_J300_locres_sharp.png supersample 3 transparentBackground true |
| 10824 | | |
| 10825 | | > save "/Users/peiyuyu/Documents/Goethe |
| 10826 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10827 | | > F_B1/20260507_SA-F_B1_workflow4.cxs" |
| 10828 | | |
| 10829 | | > open /Users/peiyuyu/Downloads/cryosparc_P7_J110_004_volume_map_sharp.mrc |
| 10830 | | |
| 10831 | | Opened cryosparc_P7_J110_004_volume_map_sharp.mrc as #64, grid size |
| 10832 | | 160,160,160, pixel 1.66, shown at level 0.28, step 1, values float32 |
| 10833 | | |
| 10834 | | > hide #!62 models |
| 10835 | | |
| 10836 | | > close #64 |
| 10837 | | |
| 10838 | | > save "/Users/peiyuyu/Documents/Goethe |
| 10839 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10840 | | > F_B1/20260507_SA-F_B1_workflow5.cxs" |
| 10841 | | |
| 10842 | | ——— End of log from Thu May 7 21:11:39 2026 ——— |
| 10843 | | |
| 10844 | | > view name session-start |
| 10845 | | |
| 10846 | | opened ChimeraX session |
| 10847 | | |
| 10848 | | > open "/Users/peiyuyu/Documents/Goethe |
| 10849 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10850 | | > F_B1/cryosparc_P4_J323_006_volume_map_sharp2.mrc" |
| 10851 | | |
| 10852 | | Opened cryosparc_P4_J323_006_volume_map_sharp2.mrc as #64, grid size |
| 10853 | | 320,320,320, pixel 0.831, shown at level 0.0329, step 2, values float32 |
| 10854 | | |
| 10855 | | > open "/Users/peiyuyu/Documents/Goethe |
| 10856 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10857 | | > F_B1/cryosparc_P4_J324_map_locres2.mrc" |
| 10858 | | |
| 10859 | | Opened cryosparc_P4_J324_map_locres2.mrc as #65, grid size 320,320,320, pixel |
| 10860 | | 0.831, shown at level 8.98, step 2, values float32 |
| 10861 | | |
| 10862 | | > open "/Users/peiyuyu/Documents/Goethe |
| 10863 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10864 | | > F_B1/cryosparc_P4_J329_006_volume_map_sharp2.mrc" |
| 10865 | | |
| 10866 | | Opened cryosparc_P4_J329_006_volume_map_sharp2.mrc as #66, grid size |
| 10867 | | 320,320,320, pixel 0.831, shown at level 0.0389, step 2, values float32 |
| 10868 | | |
| 10869 | | > open "/Users/peiyuyu/Documents/Goethe |
| 10870 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10871 | | > F_B1/cryosparc_P4_J330_map_locres2.mrc" |
| 10872 | | |
| 10873 | | Opened cryosparc_P4_J330_map_locres2.mrc as #67, grid size 320,320,320, pixel |
| 10874 | | 0.831, shown at level 9.65, step 2, values float32 |
| 10875 | | |
| 10876 | | > hide #!66 models |
| 10877 | | |
| 10878 | | > hide #!67 models |
| 10879 | | |
| 10880 | | > hide #!65 models |
| 10881 | | |
| 10882 | | > close #66 |
| 10883 | | |
| 10884 | | > close #67 |
| 10885 | | |
| 10886 | | > volume flip #64 |
| 10887 | | |
| 10888 | | Opened cryosparc_P4_J323_006_volume_map_sharp2.mrc z flip as #66, grid size |
| 10889 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 10890 | | |
| 10891 | | > volume flip #65 |
| 10892 | | |
| 10893 | | Opened cryosparc_P4_J324_map_locres2.mrc z flip as #67, grid size 320,320,320, |
| 10894 | | pixel 0.831, shown at step 1, values float32 |
| 10895 | | |
| 10896 | | > volume #66 level 0.04422 |
| 10897 | | |
| 10898 | | > volume #67 level 3.812 |
| 10899 | | |
| 10900 | | > hide #!66 models |
| 10901 | | |
| 10902 | | > hide #!67 models |
| 10903 | | |
| 10904 | | > show #!67 models |
| 10905 | | |
| 10906 | | > hide #!67 models |
| 10907 | | |
| 10908 | | > show #!66 models |
| 10909 | | |
| 10910 | | > close #64 |
| 10911 | | |
| 10912 | | > close #65 |
| 10913 | | |
| 10914 | | > rename #66 CS_P4_J323_sharp2_flip id #64 |
| 10915 | | |
| 10916 | | > save CS_P4_J323_sharp2_flip.mrc #64 |
| 10917 | | |
| 10918 | | > rename CS_P4_J324_locres2_flip id #65 |
| 10919 | | |
| 10920 | | Missing or invalid "models" argument: invalid models specifier |
| 10921 | | |
| 10922 | | > rename CS_P4_J324_locres2_flip id #65 |
| 10923 | | |
| 10924 | | Missing or invalid "models" argument: invalid models specifier |
| 10925 | | |
| 10926 | | > rename #67 CS_P4_J324_locres2_flip id #65 |
| 10927 | | |
| 10928 | | > save CS_P4_J324_locres2_flip.mrc #65 |
| 10929 | | |
| 10930 | | > hide #!64 models |
| 10931 | | |
| 10932 | | > show #!65 models |
| 10933 | | |
| 10934 | | > hide #!65 models |
| 10935 | | |
| 10936 | | > show #!64 models |
| 10937 | | |
| 10938 | | > volume #64 level 0.05469 |
| 10939 | | |
| 10940 | | > ui tool show "Surface Color" |
| 10941 | | |
| 10942 | | |
| 10943 | | ===== Log before crash end ===== |
| 10944 | | |
| 10945 | | Log: |
| 10946 | | UCSF ChimeraX version: 1.11 (2025-12-17) |
| 10947 | | © 2016-2025 Regents of the University of California. All rights reserved. |
| 10948 | | |
| 10949 | | > open "/Users/peiyuyu/Documents/Goethe |
| 10950 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 10951 | | > F_B1/20260507_SA-F_B1_workflow5.cxs" |
| 10952 | | |
| 10953 | | Opened cryosparc_P4_J216_class_03_00122_volume_sharp.mrc as #4, grid size |
| 10954 | | 128,128,128, pixel 2.08, shown at level 0.203, step 1, values float32 |
| 10955 | | Opened cryosparc_P4_J216_class_04_00122_volume_sharp.mrc as #5, grid size |
| 10956 | | 128,128,128, pixel 2.08, shown at level 0.206, step 1, values float32 |
| 10957 | | Opened CS_P4_J216_class_0_sharp_flip.mrc as #1, grid size 128,128,128, pixel |
| 10958 | | 2.08, shown at level 0.185, step 1, values float32 |
| 10959 | | Opened CS_P4_J216_class_1_sharp_flip.mrc as #2, grid size 128,128,128, pixel |
| 10960 | | 2.08, shown at level 0.203, step 1, values float32 |
| 10961 | | Opened CS_P4_J216_class_2_sharp_flip.mrc as #3, grid size 128,128,128, pixel |
| 10962 | | 2.08, shown at level 0.356, step 1, values float32 |
| 10963 | | Opened CS_P4_J219_sharp_flip.mrc as #6, grid size 80,80,80, pixel 3.32, shown |
| 10964 | | at level 1.17, step 1, values float32 |
| 10965 | | Opened CS_P4_J244_sharp_flip.mrc as #7, grid size 80,80,80, pixel 3.32, shown |
| 10966 | | at level 1.16, step 1, values float32 |
| 10967 | | Opened cryosparc_P4_J242_class_00_00122_volume_sharp.mrc as #8, grid size |
| 10968 | | 128,128,128, pixel 2.08, shown at level 0.221, step 1, values float32 |
| 10969 | | Opened cryosparc_P4_J242_class_01_00122_volume_sharp.mrc as #9, grid size |
| 10970 | | 128,128,128, pixel 2.08, shown at level 0.146, step 1, values float32 |
| 10971 | | Opened cryosparc_P4_J242_class_02_00122_volume_sharp.mrc as #10, grid size |
| 10972 | | 128,128,128, pixel 2.08, shown at level 0.195, step 1, values float32 |
| 10973 | | Opened cryosparc_P4_J242_class_03_00122_volume_sharp.mrc as #11, grid size |
| 10974 | | 128,128,128, pixel 2.08, shown at level 0.15, step 1, values float32 |
| 10975 | | Opened cryosparc_P4_J242_class_04_00122_volume_sharp.mrc as #12, grid size |
| 10976 | | 128,128,128, pixel 2.08, shown at level 0.345, step 1, values float32 |
| 10977 | | Opened cryosparc_P4_J117_class_00_00162_volume_sharp.mrc as #13, grid size |
| 10978 | | 128,128,128, pixel 2.08, shown at level 0.163, step 1, values float32 |
| 10979 | | Opened cryosparc_P4_J117_class_01_00162_volume_sharp.mrc as #14, grid size |
| 10980 | | 128,128,128, pixel 2.08, shown at level 0.187, step 1, values float32 |
| 10981 | | Opened CS_P4_J117_class_02_sharp_flip.mrc as #15, grid size 128,128,128, pixel |
| 10982 | | 2.08, shown at level 0.335, step 1, values float32 |
| 10983 | | Opened cryosparc_P4_J117_class_03_00162_volume_sharp.mrc as #16, grid size |
| 10984 | | 128,128,128, pixel 2.08, shown at level 0.179, step 1, values float32 |
| 10985 | | Opened cryosparc_P4_J117_class_04_00162_volume_sharp.mrc as #17, grid size |
| 10986 | | 128,128,128, pixel 2.08, shown at level 0.206, step 1, values float32 |
| 10987 | | Opened CS_P4_J120_sharp_flip.mrc as #18, grid size 80,80,80, pixel 3.32, shown |
| 10988 | | at level 1.29, step 1, values float32 |
| 10989 | | Opened cryosparc_P4_J169_class_00_00182_volume_sharp.mrc as #19, grid size |
| 10990 | | 128,128,128, pixel 2.08, shown at level 0.156, step 1, values float32 |
| 10991 | | Opened cryosparc_P4_J169_class_01_00182_volume_sharp.mrc as #20, grid size |
| 10992 | | 128,128,128, pixel 2.08, shown at level 0.198, step 1, values float32 |
| 10993 | | Opened cryosparc_P4_J169_class_02_00182_volume_sharp.mrc as #21, grid size |
| 10994 | | 128,128,128, pixel 2.08, shown at level 0.248, step 1, values float32 |
| 10995 | | Opened CS_P4_J169_class_03_sharp_flip.mrc as #22, grid size 128,128,128, pixel |
| 10996 | | 2.08, shown at level 0.271, step 1, values float32 |
| 10997 | | Opened cryosparc_P4_J169_class_04_00182_volume_sharp.mrc as #23, grid size |
| 10998 | | 128,128,128, pixel 2.08, shown at level 0.2, step 1, values float32 |
| 10999 | | Opened CS_P4_J176_NU_sharp_flip.mrc as #24, grid size 80,80,80, pixel 3.32, |
| 11000 | | shown at level 1.6, step 1, values float32 |
| 11001 | | Opened CS_P4_J255_sharp_flip.mrc as #25, grid size 80,80,80, pixel 3.32, shown |
| 11002 | | at level 0.649, step 1, values float32 |
| 11003 | | Opened CS_P4_J256_sharp_flip.mrc as #26, grid size 80,80,80, pixel 3.32, shown |
| 11004 | | at level 1.01, step 1, values float32 |
| 11005 | | Opened CS_P4_J257_sharp_flip.mrc as #27, grid size 80,80,80, pixel 3.32, shown |
| 11006 | | at level 1.16, step 1, values float32 |
| 11007 | | Opened CS_P4_J258_sharp_flip.mrc as #28, grid size 80,80,80, pixel 3.32, shown |
| 11008 | | at level 0.533, step 1, values float32 |
| 11009 | | Opened CS_P4_J259_sharp_flip.mrc as #29, grid size 80,80,80, pixel 3.32, shown |
| 11010 | | at level 0.567, step 1, values float32 |
| 11011 | | Opened CS_P4_J267_sharp_flip.mrc as #30, grid size 320,320,320, pixel 0.831, |
| 11012 | | shown at level 0.134, step 1, values float32 |
| 11013 | | Opened CS_P4_J277_sharp_flip.mrc as #31, grid size 320,320,320, pixel 0.831, |
| 11014 | | shown at level 0.143, step 1, values float32 |
| 11015 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc as #32, grid size |
| 11016 | | 320,320,320, pixel 0.831, shown at level 0.0548, step 2, values float32 |
| 11017 | | Opened cryosparc_P4_J285_006_volume_map_sharp.mrc as #33, grid size |
| 11018 | | 320,320,320, pixel 0.831, shown at level 0.0508, step 2, values float32 |
| 11019 | | Opened cryosparc_P4_J287_map_locres.mrc as #34, grid size 320,320,320, pixel |
| 11020 | | 0.831, shown at level 3.78, step 2, values float32 |
| 11021 | | Opened cryosparc_P4_J288_map_locres.mrc as #35, grid size 320,320,320, pixel |
| 11022 | | 0.831, shown at level 3.77, step 2, values float32 |
| 11023 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc z flip as #37, grid size |
| 11024 | | 320,320,320, pixel 0.831, shown at level 0.065, step 1, values float32 |
| 11025 | | Opened cryosparc_P4_J287_map_locres.mrc z flip as #38, grid size 320,320,320, |
| 11026 | | pixel 0.831, shown at level 3.78, step 1, values float32 |
| 11027 | | Opened cryosparc_P4_J285_006_volume_map_sharp.mrc z flip as #39, grid size |
| 11028 | | 320,320,320, pixel 0.831, shown at level 0.0664, step 1, values float32 |
| 11029 | | Opened cryosparc_P4_J288_map_locres.mrc z flip as #40, grid size 320,320,320, |
| 11030 | | pixel 0.831, shown at level 3.77, step 1, values float32 |
| 11031 | | Opened cryosparc_P4_J242_class_01_00122_volume_sharp.mrc as #42, grid size |
| 11032 | | 128,128,128, pixel 2.08, shown at level 0.254, step 1, values float32 |
| 11033 | | Opened CS_P4_J282_sharp_flip.mrc as #36, grid size 320,320,320, pixel 0.831, |
| 11034 | | shown at level 0.0602, step 2, values float32 |
| 11035 | | Opened cryosparc_P4_J169_class_02_00182_volume_sharp.mrc as #43, grid size |
| 11036 | | 128,128,128, pixel 2.08, shown at level 0.228, step 1, values float32 |
| 11037 | | Opened CS_P4_J295_sharp_flip.mrc as #44, grid size 320,320,320, pixel 0.831, |
| 11038 | | shown at level 0.0515, step 1, values float32 |
| 11039 | | Opened CS_P4_J296_locres_flip.mrc as #45, grid size 320,320,320, pixel 0.831, |
| 11040 | | shown at level 8.66, step 1, values float32 |
| 11041 | | Opened CS_P4_J300_sharp_flip.mrc as #46, grid size 320,320,320, pixel 0.831, |
| 11042 | | shown at level 0.0592, step 1, values float32 |
| 11043 | | Opened CS_P4_J301_locres_flip.mrc as #47, grid size 320,320,320, pixel 0.831, |
| 11044 | | shown at level 9.68, step 1, values float32 |
| 11045 | | Opened CS_P4_J302_sharp_flip.mrc as #48, grid size 80,80,80, pixel 3.32, shown |
| 11046 | | at level 0.612, step 1, values float32 |
| 11047 | | Opened CS_P4_J303_sharp_flip.mrc as #49, grid size 80,80,80, pixel 3.32, shown |
| 11048 | | at level 0.575, step 1, values float32 |
| 11049 | | Opened CS_P4_J304_sharp_flip.mrc as #50, grid size 80,80,80, pixel 3.32, shown |
| 11050 | | at level 1.95, step 1, values float32 |
| 11051 | | Opened CS_P4_J305_sharp_flip.mrc as #51, grid size 80,80,80, pixel 3.32, shown |
| 11052 | | at level 0.569, step 1, values float32 |
| 11053 | | Opened CS_P4_J306_sharp_flip.mrc as #52, grid size 80,80,80, pixel 3.32, shown |
| 11054 | | at level 1.03, step 1, values float32 |
| 11055 | | Opened CS_P4_J307_sharp_flip.mrc as #53, grid size 80,80,80, pixel 3.32, shown |
| 11056 | | at level 0.417, step 1, values float32 |
| 11057 | | Opened CS_P4_J308_sharp_flip.mrc as #54, grid size 80,80,80, pixel 3.32, shown |
| 11058 | | at level 0.487, step 1, values float32 |
| 11059 | | Opened CS_P4_J309_sharp_flip.mrc as #55, grid size 80,80,80, pixel 3.32, shown |
| 11060 | | at level 0.427, step 1, values float32 |
| 11061 | | Opened CS_P4_J310_sharp_flip.mrc as #56, grid size 80,80,80, pixel 3.32, shown |
| 11062 | | at level 0.719, step 1, values float32 |
| 11063 | | Opened CS_P4_J311_sharp_flip.mrc as #57, grid size 80,80,80, pixel 3.32, shown |
| 11064 | | at level 0.525, step 1, values float32 |
| 11065 | | Opened CS_P4_J323_sharp_flip.mrc as #58, grid size 320,320,320, pixel 0.831, |
| 11066 | | shown at level 0.0616, step 1, values float32 |
| 11067 | | Opened CS_P4_J324_map_locres_flip.mrc as #59, grid size 320,320,320, pixel |
| 11068 | | 0.831, shown at level 3.69, step 1, values float32 |
| 11069 | | Opened CS_P4_J329_sharp_flip.mrc as #60, grid size 320,320,320, pixel 0.831, |
| 11070 | | shown at level 0.0566, step 1, values float32 |
| 11071 | | Opened CS_P4_J330_locres_flip.mrc as #61, grid size 320,320,320, pixel 0.831, |
| 11072 | | shown at level 9.71, step 1, values float32 |
| 11073 | | Opened CS_P4_J300_sharp_flip.mrc as #62, grid size 320,320,320, pixel 0.831, |
| 11074 | | shown at level 0.0575, step 1, values float32 |
| 11075 | | Opened CS_P4_J301_locres_flip.mrc as #63, grid size 320,320,320, pixel 0.831, |
| 11076 | | shown at level 3.95, step 1, values float32 |
| 11077 | | Log from Thu May 7 21:11:39 2026UCSF ChimeraX version: 1.11 (2025-12-17) |
| 11078 | | © 2016-2025 Regents of the University of California. All rights reserved. |
| 11079 | | |
| 11080 | | > open "/Users/peiyuyu/Documents/Goethe |
| 11081 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 11082 | | > F_B1/20260507_SA-F_B1_workflow3.cxs" |
| 11083 | | |
| 11084 | | Opened cryosparc_P4_J216_class_03_00122_volume_sharp.mrc as #4, grid size |
| 11085 | | 128,128,128, pixel 2.08, shown at level 0.203, step 1, values float32 |
| 11086 | | Opened cryosparc_P4_J216_class_04_00122_volume_sharp.mrc as #5, grid size |
| 11087 | | 128,128,128, pixel 2.08, shown at level 0.206, step 1, values float32 |
| 11088 | | Opened CS_P4_J216_class_0_sharp_flip.mrc as #1, grid size 128,128,128, pixel |
| 11089 | | 2.08, shown at level 0.185, step 1, values float32 |
| 11090 | | Opened CS_P4_J216_class_1_sharp_flip.mrc as #2, grid size 128,128,128, pixel |
| 11091 | | 2.08, shown at level 0.203, step 1, values float32 |
| 11092 | | Opened CS_P4_J216_class_2_sharp_flip.mrc as #3, grid size 128,128,128, pixel |
| 11093 | | 2.08, shown at level 0.356, step 1, values float32 |
| 11094 | | Opened CS_P4_J219_sharp_flip.mrc as #6, grid size 80,80,80, pixel 3.32, shown |
| 11095 | | at level 1.17, step 1, values float32 |
| 11096 | | Opened CS_P4_J244_sharp_flip.mrc as #7, grid size 80,80,80, pixel 3.32, shown |
| 11097 | | at level 1.16, step 1, values float32 |
| 11098 | | Opened cryosparc_P4_J242_class_00_00122_volume_sharp.mrc as #8, grid size |
| 11099 | | 128,128,128, pixel 2.08, shown at level 0.221, step 1, values float32 |
| 11100 | | Opened cryosparc_P4_J242_class_01_00122_volume_sharp.mrc as #9, grid size |
| 11101 | | 128,128,128, pixel 2.08, shown at level 0.146, step 1, values float32 |
| 11102 | | Opened cryosparc_P4_J242_class_02_00122_volume_sharp.mrc as #10, grid size |
| 11103 | | 128,128,128, pixel 2.08, shown at level 0.195, step 1, values float32 |
| 11104 | | Opened cryosparc_P4_J242_class_03_00122_volume_sharp.mrc as #11, grid size |
| 11105 | | 128,128,128, pixel 2.08, shown at level 0.15, step 1, values float32 |
| 11106 | | Opened cryosparc_P4_J242_class_04_00122_volume_sharp.mrc as #12, grid size |
| 11107 | | 128,128,128, pixel 2.08, shown at level 0.345, step 1, values float32 |
| 11108 | | Opened cryosparc_P4_J117_class_00_00162_volume_sharp.mrc as #13, grid size |
| 11109 | | 128,128,128, pixel 2.08, shown at level 0.163, step 1, values float32 |
| 11110 | | Opened cryosparc_P4_J117_class_01_00162_volume_sharp.mrc as #14, grid size |
| 11111 | | 128,128,128, pixel 2.08, shown at level 0.187, step 1, values float32 |
| 11112 | | Opened CS_P4_J117_class_02_sharp_flip.mrc as #15, grid size 128,128,128, pixel |
| 11113 | | 2.08, shown at level 0.335, step 1, values float32 |
| 11114 | | Opened cryosparc_P4_J117_class_03_00162_volume_sharp.mrc as #16, grid size |
| 11115 | | 128,128,128, pixel 2.08, shown at level 0.179, step 1, values float32 |
| 11116 | | Opened cryosparc_P4_J117_class_04_00162_volume_sharp.mrc as #17, grid size |
| 11117 | | 128,128,128, pixel 2.08, shown at level 0.206, step 1, values float32 |
| 11118 | | Opened CS_P4_J120_sharp_flip.mrc as #18, grid size 80,80,80, pixel 3.32, shown |
| 11119 | | at level 1.29, step 1, values float32 |
| 11120 | | Opened cryosparc_P4_J169_class_00_00182_volume_sharp.mrc as #19, grid size |
| 11121 | | 128,128,128, pixel 2.08, shown at level 0.156, step 1, values float32 |
| 11122 | | Opened cryosparc_P4_J169_class_01_00182_volume_sharp.mrc as #20, grid size |
| 11123 | | 128,128,128, pixel 2.08, shown at level 0.198, step 1, values float32 |
| 11124 | | Opened cryosparc_P4_J169_class_02_00182_volume_sharp.mrc as #21, grid size |
| 11125 | | 128,128,128, pixel 2.08, shown at level 0.248, step 1, values float32 |
| 11126 | | Opened CS_P4_J169_class_03_sharp_flip.mrc as #22, grid size 128,128,128, pixel |
| 11127 | | 2.08, shown at level 0.271, step 1, values float32 |
| 11128 | | Opened cryosparc_P4_J169_class_04_00182_volume_sharp.mrc as #23, grid size |
| 11129 | | 128,128,128, pixel 2.08, shown at level 0.2, step 1, values float32 |
| 11130 | | Opened CS_P4_J176_NU_sharp_flip.mrc as #24, grid size 80,80,80, pixel 3.32, |
| 11131 | | shown at level 1.6, step 1, values float32 |
| 11132 | | Opened CS_P4_J255_sharp_flip.mrc as #25, grid size 80,80,80, pixel 3.32, shown |
| 11133 | | at level 0.649, step 1, values float32 |
| 11134 | | Opened CS_P4_J256_sharp_flip.mrc as #26, grid size 80,80,80, pixel 3.32, shown |
| 11135 | | at level 1.01, step 1, values float32 |
| 11136 | | Opened CS_P4_J257_sharp_flip.mrc as #27, grid size 80,80,80, pixel 3.32, shown |
| 11137 | | at level 1.16, step 1, values float32 |
| 11138 | | Opened CS_P4_J258_sharp_flip.mrc as #28, grid size 80,80,80, pixel 3.32, shown |
| 11139 | | at level 0.533, step 1, values float32 |
| 11140 | | Opened CS_P4_J259_sharp_flip.mrc as #29, grid size 80,80,80, pixel 3.32, shown |
| 11141 | | at level 0.567, step 1, values float32 |
| 11142 | | Opened CS_P4_J267_sharp_flip.mrc as #30, grid size 320,320,320, pixel 0.831, |
| 11143 | | shown at level 0.134, step 1, values float32 |
| 11144 | | Opened CS_P4_J277_sharp_flip.mrc as #31, grid size 320,320,320, pixel 0.831, |
| 11145 | | shown at level 0.143, step 1, values float32 |
| 11146 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc as #32, grid size |
| 11147 | | 320,320,320, pixel 0.831, shown at level 0.0548, step 2, values float32 |
| 11148 | | Opened cryosparc_P4_J285_006_volume_map_sharp.mrc as #33, grid size |
| 11149 | | 320,320,320, pixel 0.831, shown at level 0.0508, step 2, values float32 |
| 11150 | | Opened cryosparc_P4_J287_map_locres.mrc as #34, grid size 320,320,320, pixel |
| 11151 | | 0.831, shown at level 3.78, step 2, values float32 |
| 11152 | | Opened cryosparc_P4_J288_map_locres.mrc as #35, grid size 320,320,320, pixel |
| 11153 | | 0.831, shown at level 3.77, step 2, values float32 |
| 11154 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc z flip as #37, grid size |
| 11155 | | 320,320,320, pixel 0.831, shown at level 0.065, step 1, values float32 |
| 11156 | | Opened cryosparc_P4_J287_map_locres.mrc z flip as #38, grid size 320,320,320, |
| 11157 | | pixel 0.831, shown at level 3.78, step 1, values float32 |
| 11158 | | Opened cryosparc_P4_J285_006_volume_map_sharp.mrc z flip as #39, grid size |
| 11159 | | 320,320,320, pixel 0.831, shown at level 0.0664, step 1, values float32 |
| 11160 | | Opened cryosparc_P4_J288_map_locres.mrc z flip as #40, grid size 320,320,320, |
| 11161 | | pixel 0.831, shown at level 3.77, step 1, values float32 |
| 11162 | | Opened cryosparc_P4_J242_class_01_00122_volume_sharp.mrc as #42, grid size |
| 11163 | | 128,128,128, pixel 2.08, shown at level 0.254, step 1, values float32 |
| 11164 | | Opened CS_P4_J282_sharp_flip.mrc as #36, grid size 320,320,320, pixel 0.831, |
| 11165 | | shown at level 0.0602, step 2, values float32 |
| 11166 | | Opened cryosparc_P4_J169_class_02_00182_volume_sharp.mrc as #43, grid size |
| 11167 | | 128,128,128, pixel 2.08, shown at level 0.228, step 1, values float32 |
| 11168 | | Opened CS_P4_J295_sharp_flip.mrc as #44, grid size 320,320,320, pixel 0.831, |
| 11169 | | shown at level 0.0515, step 1, values float32 |
| 11170 | | Opened CS_P4_J296_locres_flip.mrc as #45, grid size 320,320,320, pixel 0.831, |
| 11171 | | shown at level 8.66, step 1, values float32 |
| 11172 | | Opened CS_P4_J300_sharp_flip.mrc as #46, grid size 320,320,320, pixel 0.831, |
| 11173 | | shown at level 0.0592, step 1, values float32 |
| 11174 | | Opened CS_P4_J301_locres_flip.mrc as #47, grid size 320,320,320, pixel 0.831, |
| 11175 | | shown at level 9.68, step 1, values float32 |
| 11176 | | Opened CS_P4_J302_sharp_flip.mrc as #48, grid size 80,80,80, pixel 3.32, shown |
| 11177 | | at level 0.612, step 1, values float32 |
| 11178 | | Opened CS_P4_J303_sharp_flip.mrc as #49, grid size 80,80,80, pixel 3.32, shown |
| 11179 | | at level 0.575, step 1, values float32 |
| 11180 | | Opened CS_P4_J304_sharp_flip.mrc as #50, grid size 80,80,80, pixel 3.32, shown |
| 11181 | | at level 1.95, step 1, values float32 |
| 11182 | | Opened CS_P4_J305_sharp_flip.mrc as #51, grid size 80,80,80, pixel 3.32, shown |
| 11183 | | at level 0.569, step 1, values float32 |
| 11184 | | Opened CS_P4_J306_sharp_flip.mrc as #52, grid size 80,80,80, pixel 3.32, shown |
| 11185 | | at level 1.03, step 1, values float32 |
| 11186 | | Opened CS_P4_J307_sharp_flip.mrc as #53, grid size 80,80,80, pixel 3.32, shown |
| 11187 | | at level 0.417, step 1, values float32 |
| 11188 | | Opened CS_P4_J308_sharp_flip.mrc as #54, grid size 80,80,80, pixel 3.32, shown |
| 11189 | | at level 0.487, step 1, values float32 |
| 11190 | | Opened CS_P4_J309_sharp_flip.mrc as #55, grid size 80,80,80, pixel 3.32, shown |
| 11191 | | at level 0.427, step 1, values float32 |
| 11192 | | Opened CS_P4_J310_sharp_flip.mrc as #56, grid size 80,80,80, pixel 3.32, shown |
| 11193 | | at level 0.719, step 1, values float32 |
| 11194 | | Opened CS_P4_J311_sharp_flip.mrc as #57, grid size 80,80,80, pixel 3.32, shown |
| 11195 | | at level 0.525, step 1, values float32 |
| 11196 | | Opened CS_P4_J323_sharp_flip.mrc as #58, grid size 320,320,320, pixel 0.831, |
| 11197 | | shown at level 0.0616, step 1, values float32 |
| 11198 | | Opened CS_P4_J324_map_locres_flip.mrc as #59, grid size 320,320,320, pixel |
| 11199 | | 0.831, shown at level 3.69, step 1, values float32 |
| 11200 | | Opened CS_P4_J329_sharp_flip.mrc as #60, grid size 320,320,320, pixel 0.831, |
| 11201 | | shown at level 0.0566, step 1, values float32 |
| 11202 | | Opened CS_P4_J330_locres_flip.mrc as #61, grid size 320,320,320, pixel 0.831, |
| 11203 | | shown at level 9.71, step 1, values float32 |
| 11204 | | Log from Thu May 7 15:58:17 2026UCSF ChimeraX version: 1.11 (2025-12-17) |
| 11205 | | © 2016-2025 Regents of the University of California. All rights reserved. |
| 11206 | | |
| 11207 | | > open "/Users/peiyuyu/Documents/Goethe |
| 11208 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 11209 | | > F_B1/20260506_SA-F_B1_workflow.cxs" |
| 11210 | | |
| 11211 | | Opened cryosparc_P4_J216_class_03_00122_volume_sharp.mrc as #4, grid size |
| 11212 | | 128,128,128, pixel 2.08, shown at level 0.203, step 1, values float32 |
| 11213 | | Opened cryosparc_P4_J216_class_04_00122_volume_sharp.mrc as #5, grid size |
| 11214 | | 128,128,128, pixel 2.08, shown at level 0.206, step 1, values float32 |
| 11215 | | Opened CS_P4_J216_class_0_sharp_flip.mrc as #1, grid size 128,128,128, pixel |
| 11216 | | 2.08, shown at level 0.185, step 1, values float32 |
| 11217 | | Opened CS_P4_J216_class_1_sharp_flip.mrc as #2, grid size 128,128,128, pixel |
| 11218 | | 2.08, shown at level 0.203, step 1, values float32 |
| 11219 | | Opened CS_P4_J216_class_2_sharp_flip.mrc as #3, grid size 128,128,128, pixel |
| 11220 | | 2.08, shown at level 0.356, step 1, values float32 |
| 11221 | | Opened CS_P4_J219_sharp_flip.mrc as #6, grid size 80,80,80, pixel 3.32, shown |
| 11222 | | at level 1.17, step 1, values float32 |
| 11223 | | Opened CS_P4_J244_sharp_flip.mrc as #7, grid size 80,80,80, pixel 3.32, shown |
| 11224 | | at level 1.16, step 1, values float32 |
| 11225 | | Opened cryosparc_P4_J242_class_00_00122_volume_sharp.mrc as #8, grid size |
| 11226 | | 128,128,128, pixel 2.08, shown at level 0.221, step 1, values float32 |
| 11227 | | Opened cryosparc_P4_J242_class_01_00122_volume_sharp.mrc as #9, grid size |
| 11228 | | 128,128,128, pixel 2.08, shown at level 0.146, step 1, values float32 |
| 11229 | | Opened cryosparc_P4_J242_class_02_00122_volume_sharp.mrc as #10, grid size |
| 11230 | | 128,128,128, pixel 2.08, shown at level 0.195, step 1, values float32 |
| 11231 | | Opened cryosparc_P4_J242_class_03_00122_volume_sharp.mrc as #11, grid size |
| 11232 | | 128,128,128, pixel 2.08, shown at level 0.15, step 1, values float32 |
| 11233 | | Opened cryosparc_P4_J242_class_04_00122_volume_sharp.mrc as #12, grid size |
| 11234 | | 128,128,128, pixel 2.08, shown at level 0.345, step 1, values float32 |
| 11235 | | Opened cryosparc_P4_J117_class_00_00162_volume_sharp.mrc as #13, grid size |
| 11236 | | 128,128,128, pixel 2.08, shown at level 0.163, step 1, values float32 |
| 11237 | | Opened cryosparc_P4_J117_class_01_00162_volume_sharp.mrc as #14, grid size |
| 11238 | | 128,128,128, pixel 2.08, shown at level 0.187, step 1, values float32 |
| 11239 | | Opened CS_P4_J117_class_02_sharp_flip.mrc as #15, grid size 128,128,128, pixel |
| 11240 | | 2.08, shown at level 0.335, step 1, values float32 |
| 11241 | | Opened cryosparc_P4_J117_class_03_00162_volume_sharp.mrc as #16, grid size |
| 11242 | | 128,128,128, pixel 2.08, shown at level 0.179, step 1, values float32 |
| 11243 | | Opened cryosparc_P4_J117_class_04_00162_volume_sharp.mrc as #17, grid size |
| 11244 | | 128,128,128, pixel 2.08, shown at level 0.206, step 1, values float32 |
| 11245 | | Opened CS_P4_J120_sharp_flip.mrc as #18, grid size 80,80,80, pixel 3.32, shown |
| 11246 | | at level 1.29, step 1, values float32 |
| 11247 | | Opened cryosparc_P4_J169_class_00_00182_volume_sharp.mrc as #19, grid size |
| 11248 | | 128,128,128, pixel 2.08, shown at level 0.156, step 1, values float32 |
| 11249 | | Opened cryosparc_P4_J169_class_01_00182_volume_sharp.mrc as #20, grid size |
| 11250 | | 128,128,128, pixel 2.08, shown at level 0.198, step 1, values float32 |
| 11251 | | Opened cryosparc_P4_J169_class_02_00182_volume_sharp.mrc as #21, grid size |
| 11252 | | 128,128,128, pixel 2.08, shown at level 0.248, step 1, values float32 |
| 11253 | | Opened CS_P4_J169_class_03_sharp_flip.mrc as #22, grid size 128,128,128, pixel |
| 11254 | | 2.08, shown at level 0.271, step 1, values float32 |
| 11255 | | Opened cryosparc_P4_J169_class_04_00182_volume_sharp.mrc as #23, grid size |
| 11256 | | 128,128,128, pixel 2.08, shown at level 0.2, step 1, values float32 |
| 11257 | | Opened CS_P4_J176_NU_sharp_flip.mrc as #24, grid size 80,80,80, pixel 3.32, |
| 11258 | | shown at level 1.6, step 1, values float32 |
| 11259 | | Opened CS_P4_J255_sharp_flip.mrc as #25, grid size 80,80,80, pixel 3.32, shown |
| 11260 | | at level 0.649, step 1, values float32 |
| 11261 | | Opened CS_P4_J256_sharp_flip.mrc as #26, grid size 80,80,80, pixel 3.32, shown |
| 11262 | | at level 1.01, step 1, values float32 |
| 11263 | | Opened CS_P4_J257_sharp_flip.mrc as #27, grid size 80,80,80, pixel 3.32, shown |
| 11264 | | at level 1.16, step 1, values float32 |
| 11265 | | Opened CS_P4_J258_sharp_flip.mrc as #28, grid size 80,80,80, pixel 3.32, shown |
| 11266 | | at level 0.533, step 1, values float32 |
| 11267 | | Opened CS_P4_J259_sharp_flip.mrc as #29, grid size 80,80,80, pixel 3.32, shown |
| 11268 | | at level 0.567, step 1, values float32 |
| 11269 | | Opened CS_P4_J267_sharp_flip.mrc as #30, grid size 320,320,320, pixel 0.831, |
| 11270 | | shown at level 0.134, step 1, values float32 |
| 11271 | | Opened CS_P4_J277_sharp_flip.mrc as #31, grid size 320,320,320, pixel 0.831, |
| 11272 | | shown at level 0.143, step 1, values float32 |
| 11273 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc as #32, grid size |
| 11274 | | 320,320,320, pixel 0.831, shown at level 0.0548, step 2, values float32 |
| 11275 | | Opened cryosparc_P4_J285_006_volume_map_sharp.mrc as #33, grid size |
| 11276 | | 320,320,320, pixel 0.831, shown at level 0.0508, step 2, values float32 |
| 11277 | | Opened cryosparc_P4_J287_map_locres.mrc as #34, grid size 320,320,320, pixel |
| 11278 | | 0.831, shown at level 3.78, step 2, values float32 |
| 11279 | | Opened cryosparc_P4_J288_map_locres.mrc as #35, grid size 320,320,320, pixel |
| 11280 | | 0.831, shown at level 3.77, step 2, values float32 |
| 11281 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc z flip as #37, grid size |
| 11282 | | 320,320,320, pixel 0.831, shown at level 0.065, step 1, values float32 |
| 11283 | | Opened cryosparc_P4_J287_map_locres.mrc z flip as #38, grid size 320,320,320, |
| 11284 | | pixel 0.831, shown at level 3.78, step 1, values float32 |
| 11285 | | Opened cryosparc_P4_J285_006_volume_map_sharp.mrc z flip as #39, grid size |
| 11286 | | 320,320,320, pixel 0.831, shown at level 0.0664, step 1, values float32 |
| 11287 | | Opened cryosparc_P4_J288_map_locres.mrc z flip as #40, grid size 320,320,320, |
| 11288 | | pixel 0.831, shown at level 3.77, step 1, values float32 |
| 11289 | | Opened cryosparc_P4_J242_class_01_00122_volume_sharp.mrc as #42, grid size |
| 11290 | | 128,128,128, pixel 2.08, shown at level 0.254, step 1, values float32 |
| 11291 | | Opened CS_P4_J282_sharp_flip.mrc as #36, grid size 320,320,320, pixel 0.831, |
| 11292 | | shown at level 0.0602, step 2, values float32 |
| 11293 | | Opened cryosparc_P4_J169_class_02_00182_volume_sharp.mrc as #43, grid size |
| 11294 | | 128,128,128, pixel 2.08, shown at level 0.228, step 1, values float32 |
| 11295 | | Opened CS_P4_J295_sharp_flip.mrc as #44, grid size 320,320,320, pixel 0.831, |
| 11296 | | shown at level 0.0515, step 1, values float32 |
| 11297 | | Opened CS_P4_J296_locres_flip.mrc as #45, grid size 320,320,320, pixel 0.831, |
| 11298 | | shown at level 8.66, step 1, values float32 |
| 11299 | | Opened CS_P4_J300_sharp_flip.mrc as #46, grid size 320,320,320, pixel 0.831, |
| 11300 | | shown at level 0.0592, step 1, values float32 |
| 11301 | | Opened CS_P4_J301_locres_flip.mrc as #47, grid size 320,320,320, pixel 0.831, |
| 11302 | | shown at level 9.68, step 1, values float32 |
| 11303 | | Opened CS_P4_J302_sharp_flip.mrc as #48, grid size 80,80,80, pixel 3.32, shown |
| 11304 | | at level 0.612, step 1, values float32 |
| 11305 | | Opened CS_P4_J303_sharp_flip.mrc as #49, grid size 80,80,80, pixel 3.32, shown |
| 11306 | | at level 0.575, step 1, values float32 |
| 11307 | | Opened CS_P4_J304_sharp_flip.mrc as #50, grid size 80,80,80, pixel 3.32, shown |
| 11308 | | at level 1.95, step 1, values float32 |
| 11309 | | Opened CS_P4_J305_sharp_flip.mrc as #51, grid size 80,80,80, pixel 3.32, shown |
| 11310 | | at level 0.569, step 1, values float32 |
| 11311 | | Opened CS_P4_J306_sharp_flip.mrc as #52, grid size 80,80,80, pixel 3.32, shown |
| 11312 | | at level 1.03, step 1, values float32 |
| 11313 | | Opened CS_P4_J307_sharp_flip.mrc as #53, grid size 80,80,80, pixel 3.32, shown |
| 11314 | | at level 0.417, step 1, values float32 |
| 11315 | | Opened CS_P4_J308_sharp_flip.mrc as #54, grid size 80,80,80, pixel 3.32, shown |
| 11316 | | at level 0.487, step 1, values float32 |
| 11317 | | Opened CS_P4_J309_sharp_flip.mrc as #55, grid size 80,80,80, pixel 3.32, shown |
| 11318 | | at level 0.427, step 1, values float32 |
| 11319 | | Opened CS_P4_J310_sharp_flip.mrc as #56, grid size 80,80,80, pixel 3.32, shown |
| 11320 | | at level 0.719, step 1, values float32 |
| 11321 | | Opened CS_P4_J311_sharp_flip.mrc as #57, grid size 80,80,80, pixel 3.32, shown |
| 11322 | | at level 0.525, step 1, values float32 |
| 11323 | | Log from Wed May 6 15:19:28 2026UCSF ChimeraX version: 1.11 (2025-12-17) |
| 11324 | | © 2016-2025 Regents of the University of California. All rights reserved. |
| 11325 | | |
| 11326 | | > open "/Users/peiyuyu/Documents/Goethe |
| 11327 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 11328 | | > F_B1/20260505_SA-F_B1_workflow.cxs" |
| 11329 | | |
| 11330 | | Opened cryosparc_P4_J216_class_03_00122_volume_sharp.mrc as #4, grid size |
| 11331 | | 128,128,128, pixel 2.08, shown at level 0.203, step 1, values float32 |
| 11332 | | Opened cryosparc_P4_J216_class_04_00122_volume_sharp.mrc as #5, grid size |
| 11333 | | 128,128,128, pixel 2.08, shown at level 0.206, step 1, values float32 |
| 11334 | | Opened CS_P4_J216_class_0_sharp_flip.mrc as #1, grid size 128,128,128, pixel |
| 11335 | | 2.08, shown at level 0.185, step 1, values float32 |
| 11336 | | Opened CS_P4_J216_class_1_sharp_flip.mrc as #2, grid size 128,128,128, pixel |
| 11337 | | 2.08, shown at level 0.203, step 1, values float32 |
| 11338 | | Opened CS_P4_J216_class_2_sharp_flip.mrc as #3, grid size 128,128,128, pixel |
| 11339 | | 2.08, shown at level 0.356, step 1, values float32 |
| 11340 | | Opened CS_P4_J219_sharp_flip.mrc as #6, grid size 80,80,80, pixel 3.32, shown |
| 11341 | | at level 1.17, step 1, values float32 |
| 11342 | | Opened CS_P4_J244_sharp_flip.mrc as #7, grid size 80,80,80, pixel 3.32, shown |
| 11343 | | at level 1.16, step 1, values float32 |
| 11344 | | Opened cryosparc_P4_J242_class_00_00122_volume_sharp.mrc as #8, grid size |
| 11345 | | 128,128,128, pixel 2.08, shown at level 0.221, step 1, values float32 |
| 11346 | | Opened cryosparc_P4_J242_class_01_00122_volume_sharp.mrc as #9, grid size |
| 11347 | | 128,128,128, pixel 2.08, shown at level 0.146, step 1, values float32 |
| 11348 | | Opened cryosparc_P4_J242_class_02_00122_volume_sharp.mrc as #10, grid size |
| 11349 | | 128,128,128, pixel 2.08, shown at level 0.195, step 1, values float32 |
| 11350 | | Opened cryosparc_P4_J242_class_03_00122_volume_sharp.mrc as #11, grid size |
| 11351 | | 128,128,128, pixel 2.08, shown at level 0.15, step 1, values float32 |
| 11352 | | Opened cryosparc_P4_J242_class_04_00122_volume_sharp.mrc as #12, grid size |
| 11353 | | 128,128,128, pixel 2.08, shown at level 0.345, step 1, values float32 |
| 11354 | | Opened cryosparc_P4_J117_class_00_00162_volume_sharp.mrc as #13, grid size |
| 11355 | | 128,128,128, pixel 2.08, shown at level 0.163, step 1, values float32 |
| 11356 | | Opened cryosparc_P4_J117_class_01_00162_volume_sharp.mrc as #14, grid size |
| 11357 | | 128,128,128, pixel 2.08, shown at level 0.187, step 1, values float32 |
| 11358 | | Opened CS_P4_J117_class_02_sharp_flip.mrc as #15, grid size 128,128,128, pixel |
| 11359 | | 2.08, shown at level 0.335, step 1, values float32 |
| 11360 | | Opened cryosparc_P4_J117_class_03_00162_volume_sharp.mrc as #16, grid size |
| 11361 | | 128,128,128, pixel 2.08, shown at level 0.179, step 1, values float32 |
| 11362 | | Opened cryosparc_P4_J117_class_04_00162_volume_sharp.mrc as #17, grid size |
| 11363 | | 128,128,128, pixel 2.08, shown at level 0.206, step 1, values float32 |
| 11364 | | Opened CS_P4_J120_sharp_flip.mrc as #18, grid size 80,80,80, pixel 3.32, shown |
| 11365 | | at level 1.29, step 1, values float32 |
| 11366 | | Opened cryosparc_P4_J169_class_00_00182_volume_sharp.mrc as #19, grid size |
| 11367 | | 128,128,128, pixel 2.08, shown at level 0.156, step 1, values float32 |
| 11368 | | Opened cryosparc_P4_J169_class_01_00182_volume_sharp.mrc as #20, grid size |
| 11369 | | 128,128,128, pixel 2.08, shown at level 0.198, step 1, values float32 |
| 11370 | | Opened cryosparc_P4_J169_class_02_00182_volume_sharp.mrc as #21, grid size |
| 11371 | | 128,128,128, pixel 2.08, shown at level 0.248, step 1, values float32 |
| 11372 | | Opened CS_P4_J169_class_03_sharp_flip.mrc as #22, grid size 128,128,128, pixel |
| 11373 | | 2.08, shown at level 0.271, step 1, values float32 |
| 11374 | | Opened cryosparc_P4_J169_class_04_00182_volume_sharp.mrc as #23, grid size |
| 11375 | | 128,128,128, pixel 2.08, shown at level 0.2, step 1, values float32 |
| 11376 | | Opened CS_P4_J176_NU_sharp_flip.mrc as #24, grid size 80,80,80, pixel 3.32, |
| 11377 | | shown at level 1.6, step 1, values float32 |
| 11378 | | Opened CS_P4_J255_sharp_flip.mrc as #25, grid size 80,80,80, pixel 3.32, shown |
| 11379 | | at level 0.649, step 1, values float32 |
| 11380 | | Opened CS_P4_J256_sharp_flip.mrc as #26, grid size 80,80,80, pixel 3.32, shown |
| 11381 | | at level 1.01, step 1, values float32 |
| 11382 | | Opened CS_P4_J257_sharp_flip.mrc as #27, grid size 80,80,80, pixel 3.32, shown |
| 11383 | | at level 1.16, step 1, values float32 |
| 11384 | | Opened CS_P4_J258_sharp_flip.mrc as #28, grid size 80,80,80, pixel 3.32, shown |
| 11385 | | at level 0.533, step 1, values float32 |
| 11386 | | Opened CS_P4_J259_sharp_flip.mrc as #29, grid size 80,80,80, pixel 3.32, shown |
| 11387 | | at level 0.567, step 1, values float32 |
| 11388 | | Opened CS_P4_J267_sharp_flip.mrc as #30, grid size 320,320,320, pixel 0.831, |
| 11389 | | shown at level 0.134, step 1, values float32 |
| 11390 | | Opened CS_P4_J277_sharp_flip.mrc as #31, grid size 320,320,320, pixel 0.831, |
| 11391 | | shown at level 0.143, step 1, values float32 |
| 11392 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc as #32, grid size |
| 11393 | | 320,320,320, pixel 0.831, shown at level 0.0548, step 2, values float32 |
| 11394 | | Opened cryosparc_P4_J285_006_volume_map_sharp.mrc as #33, grid size |
| 11395 | | 320,320,320, pixel 0.831, shown at level 0.0508, step 2, values float32 |
| 11396 | | Opened cryosparc_P4_J287_map_locres.mrc as #34, grid size 320,320,320, pixel |
| 11397 | | 0.831, shown at level 3.78, step 2, values float32 |
| 11398 | | Opened cryosparc_P4_J288_map_locres.mrc as #35, grid size 320,320,320, pixel |
| 11399 | | 0.831, shown at level 3.77, step 2, values float32 |
| 11400 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc z flip as #37, grid size |
| 11401 | | 320,320,320, pixel 0.831, shown at level 0.065, step 1, values float32 |
| 11402 | | Opened cryosparc_P4_J287_map_locres.mrc z flip as #38, grid size 320,320,320, |
| 11403 | | pixel 0.831, shown at level 3.78, step 1, values float32 |
| 11404 | | Opened cryosparc_P4_J285_006_volume_map_sharp.mrc z flip as #39, grid size |
| 11405 | | 320,320,320, pixel 0.831, shown at level 0.0664, step 1, values float32 |
| 11406 | | Opened cryosparc_P4_J288_map_locres.mrc z flip as #40, grid size 320,320,320, |
| 11407 | | pixel 0.831, shown at level 3.77, step 1, values float32 |
| 11408 | | Opened cryosparc_P4_J242_class_01_00122_volume_sharp.mrc as #42, grid size |
| 11409 | | 128,128,128, pixel 2.08, shown at level 0.254, step 1, values float32 |
| 11410 | | Opened CS_P4_J282_sharp_flip.mrc as #36, grid size 320,320,320, pixel 0.831, |
| 11411 | | shown at level 0.0602, step 2, values float32 |
| 11412 | | Opened cryosparc_P4_J169_class_02_00182_volume_sharp.mrc as #43, grid size |
| 11413 | | 128,128,128, pixel 2.08, shown at level 0.228, step 1, values float32 |
| 11414 | | Opened CS_P4_J295_sharp_flip.mrc as #44, grid size 320,320,320, pixel 0.831, |
| 11415 | | shown at level 0.0515, step 1, values float32 |
| 11416 | | Opened CS_P4_J296_locres_flip.mrc as #45, grid size 320,320,320, pixel 0.831, |
| 11417 | | shown at level 8.66, step 1, values float32 |
| 11418 | | Opened CS_P4_J300_sharp_flip.mrc as #46, grid size 320,320,320, pixel 0.831, |
| 11419 | | shown at level 0.0592, step 1, values float32 |
| 11420 | | Opened CS_P4_J301_locres_flip.mrc as #47, grid size 320,320,320, pixel 0.831, |
| 11421 | | shown at level 9.68, step 1, values float32 |
| 11422 | | Log from Tue May 5 09:31:57 2026UCSF ChimeraX version: 1.11 (2025-12-17) |
| 11423 | | © 2016-2025 Regents of the University of California. All rights reserved. |
| 11424 | | |
| 11425 | | > open "/Users/peiyuyu/Documents/Goethe |
| 11426 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 11427 | | > F_B1/20260503_SA-F_B1_workflow4.cxs" |
| 11428 | | |
| 11429 | | Opened cryosparc_P4_J216_class_03_00122_volume_sharp.mrc as #4, grid size |
| 11430 | | 128,128,128, pixel 2.08, shown at level 0.203, step 1, values float32 |
| 11431 | | Opened cryosparc_P4_J216_class_04_00122_volume_sharp.mrc as #5, grid size |
| 11432 | | 128,128,128, pixel 2.08, shown at level 0.206, step 1, values float32 |
| 11433 | | Opened CS_P4_J216_class_0_sharp_flip.mrc as #1, grid size 128,128,128, pixel |
| 11434 | | 2.08, shown at level 0.185, step 1, values float32 |
| 11435 | | Opened CS_P4_J216_class_1_sharp_flip.mrc as #2, grid size 128,128,128, pixel |
| 11436 | | 2.08, shown at level 0.203, step 1, values float32 |
| 11437 | | Opened CS_P4_J216_class_2_sharp_flip.mrc as #3, grid size 128,128,128, pixel |
| 11438 | | 2.08, shown at level 0.356, step 1, values float32 |
| 11439 | | Opened CS_P4_J219_sharp_flip.mrc as #6, grid size 80,80,80, pixel 3.32, shown |
| 11440 | | at level 1.17, step 1, values float32 |
| 11441 | | Opened CS_P4_J244_sharp_flip.mrc as #7, grid size 80,80,80, pixel 3.32, shown |
| 11442 | | at level 1.16, step 1, values float32 |
| 11443 | | Opened cryosparc_P4_J242_class_00_00122_volume_sharp.mrc as #8, grid size |
| 11444 | | 128,128,128, pixel 2.08, shown at level 0.221, step 1, values float32 |
| 11445 | | Opened cryosparc_P4_J242_class_01_00122_volume_sharp.mrc as #9, grid size |
| 11446 | | 128,128,128, pixel 2.08, shown at level 0.146, step 1, values float32 |
| 11447 | | Opened cryosparc_P4_J242_class_02_00122_volume_sharp.mrc as #10, grid size |
| 11448 | | 128,128,128, pixel 2.08, shown at level 0.195, step 1, values float32 |
| 11449 | | Opened cryosparc_P4_J242_class_03_00122_volume_sharp.mrc as #11, grid size |
| 11450 | | 128,128,128, pixel 2.08, shown at level 0.15, step 1, values float32 |
| 11451 | | Opened cryosparc_P4_J242_class_04_00122_volume_sharp.mrc as #12, grid size |
| 11452 | | 128,128,128, pixel 2.08, shown at level 0.345, step 1, values float32 |
| 11453 | | Opened cryosparc_P4_J117_class_00_00162_volume_sharp.mrc as #13, grid size |
| 11454 | | 128,128,128, pixel 2.08, shown at level 0.163, step 1, values float32 |
| 11455 | | Opened cryosparc_P4_J117_class_01_00162_volume_sharp.mrc as #14, grid size |
| 11456 | | 128,128,128, pixel 2.08, shown at level 0.187, step 1, values float32 |
| 11457 | | Opened CS_P4_J117_class_02_sharp_flip.mrc as #15, grid size 128,128,128, pixel |
| 11458 | | 2.08, shown at level 0.335, step 1, values float32 |
| 11459 | | Opened cryosparc_P4_J117_class_03_00162_volume_sharp.mrc as #16, grid size |
| 11460 | | 128,128,128, pixel 2.08, shown at level 0.179, step 1, values float32 |
| 11461 | | Opened cryosparc_P4_J117_class_04_00162_volume_sharp.mrc as #17, grid size |
| 11462 | | 128,128,128, pixel 2.08, shown at level 0.206, step 1, values float32 |
| 11463 | | Opened CS_P4_J120_sharp_flip.mrc as #18, grid size 80,80,80, pixel 3.32, shown |
| 11464 | | at level 1.29, step 1, values float32 |
| 11465 | | Opened cryosparc_P4_J169_class_00_00182_volume_sharp.mrc as #19, grid size |
| 11466 | | 128,128,128, pixel 2.08, shown at level 0.156, step 1, values float32 |
| 11467 | | Opened cryosparc_P4_J169_class_01_00182_volume_sharp.mrc as #20, grid size |
| 11468 | | 128,128,128, pixel 2.08, shown at level 0.198, step 1, values float32 |
| 11469 | | Opened cryosparc_P4_J169_class_02_00182_volume_sharp.mrc as #21, grid size |
| 11470 | | 128,128,128, pixel 2.08, shown at level 0.248, step 1, values float32 |
| 11471 | | Opened CS_P4_J169_class_03_sharp_flip.mrc as #22, grid size 128,128,128, pixel |
| 11472 | | 2.08, shown at level 0.271, step 1, values float32 |
| 11473 | | Opened cryosparc_P4_J169_class_04_00182_volume_sharp.mrc as #23, grid size |
| 11474 | | 128,128,128, pixel 2.08, shown at level 0.2, step 1, values float32 |
| 11475 | | Opened CS_P4_J176_NU_sharp_flip.mrc as #24, grid size 80,80,80, pixel 3.32, |
| 11476 | | shown at level 1.6, step 1, values float32 |
| 11477 | | Opened CS_P4_J255_sharp_flip.mrc as #25, grid size 80,80,80, pixel 3.32, shown |
| 11478 | | at level 0.649, step 1, values float32 |
| 11479 | | Opened CS_P4_J256_sharp_flip.mrc as #26, grid size 80,80,80, pixel 3.32, shown |
| 11480 | | at level 1.01, step 1, values float32 |
| 11481 | | Opened CS_P4_J257_sharp_flip.mrc as #27, grid size 80,80,80, pixel 3.32, shown |
| 11482 | | at level 1.16, step 1, values float32 |
| 11483 | | Opened CS_P4_J258_sharp_flip.mrc as #28, grid size 80,80,80, pixel 3.32, shown |
| 11484 | | at level 0.533, step 1, values float32 |
| 11485 | | Opened CS_P4_J259_sharp_flip.mrc as #29, grid size 80,80,80, pixel 3.32, shown |
| 11486 | | at level 0.567, step 1, values float32 |
| 11487 | | Opened CS_P4_J267_sharp_flip.mrc as #30, grid size 320,320,320, pixel 0.831, |
| 11488 | | shown at level 0.134, step 1, values float32 |
| 11489 | | Opened CS_P4_J277_sharp_flip.mrc as #31, grid size 320,320,320, pixel 0.831, |
| 11490 | | shown at level 0.143, step 1, values float32 |
| 11491 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc as #32, grid size |
| 11492 | | 320,320,320, pixel 0.831, shown at level 0.0548, step 2, values float32 |
| 11493 | | Opened cryosparc_P4_J285_006_volume_map_sharp.mrc as #33, grid size |
| 11494 | | 320,320,320, pixel 0.831, shown at level 0.0508, step 2, values float32 |
| 11495 | | Opened cryosparc_P4_J287_map_locres.mrc as #34, grid size 320,320,320, pixel |
| 11496 | | 0.831, shown at level 3.78, step 2, values float32 |
| 11497 | | Opened cryosparc_P4_J288_map_locres.mrc as #35, grid size 320,320,320, pixel |
| 11498 | | 0.831, shown at level 3.77, step 2, values float32 |
| 11499 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc z flip as #37, grid size |
| 11500 | | 320,320,320, pixel 0.831, shown at level 0.065, step 1, values float32 |
| 11501 | | Opened cryosparc_P4_J287_map_locres.mrc z flip as #38, grid size 320,320,320, |
| 11502 | | pixel 0.831, shown at level 3.78, step 1, values float32 |
| 11503 | | Opened cryosparc_P4_J285_006_volume_map_sharp.mrc z flip as #39, grid size |
| 11504 | | 320,320,320, pixel 0.831, shown at level 0.0664, step 1, values float32 |
| 11505 | | Opened cryosparc_P4_J288_map_locres.mrc z flip as #40, grid size 320,320,320, |
| 11506 | | pixel 0.831, shown at level 3.77, step 1, values float32 |
| 11507 | | Opened cryosparc_P4_J242_class_01_00122_volume_sharp.mrc as #42, grid size |
| 11508 | | 128,128,128, pixel 2.08, shown at level 0.254, step 1, values float32 |
| 11509 | | Opened CS_P4_J282_sharp_flip.mrc as #36, grid size 320,320,320, pixel 0.831, |
| 11510 | | shown at level 0.0602, step 2, values float32 |
| 11511 | | Opened cryosparc_P4_J169_class_02_00182_volume_sharp.mrc as #43, grid size |
| 11512 | | 128,128,128, pixel 2.08, shown at level 0.228, step 1, values float32 |
| 11513 | | Log from Sun May 3 17:29:06 2026UCSF ChimeraX version: 1.11 (2025-12-17) |
| 11514 | | © 2016-2025 Regents of the University of California. All rights reserved. |
| 11515 | | How to cite UCSF ChimeraX |
| 11516 | | |
| 11517 | | > open "/Users/peiyuyu/Documents/Goethe |
| 11518 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 11519 | | > F_B1/cryosparc_P4_J216_class_00_00122_volume_sharp.mrc" |
| 11520 | | |
| 11521 | | Opened cryosparc_P4_J216_class_00_00122_volume_sharp.mrc as #1, grid size |
| 11522 | | 128,128,128, pixel 2.08, shown at level 0.204, step 1, values float32 |
| 11523 | | |
| 11524 | | > open "/Users/peiyuyu/Documents/Goethe |
| 11525 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 11526 | | > F_B1/cryosparc_P4_J216_class_01_00122_volume_sharp.mrc" |
| 11527 | | |
| 11528 | | Opened cryosparc_P4_J216_class_01_00122_volume_sharp.mrc as #2, grid size |
| 11529 | | 128,128,128, pixel 2.08, shown at level 0.218, step 1, values float32 |
| 11530 | | |
| 11531 | | > open "/Users/peiyuyu/Documents/Goethe |
| 11532 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 11533 | | > F_B1/cryosparc_P4_J216_class_02_00122_volume_sharp.mrc" |
| 11534 | | |
| 11535 | | Opened cryosparc_P4_J216_class_02_00122_volume_sharp.mrc as #3, grid size |
| 11536 | | 128,128,128, pixel 2.08, shown at level 0.263, step 1, values float32 |
| 11537 | | |
| 11538 | | > open "/Users/peiyuyu/Documents/Goethe |
| 11539 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 11540 | | > F_B1/cryosparc_P4_J216_class_03_00122_volume_sharp.mrc" |
| 11541 | | |
| 11542 | | Opened cryosparc_P4_J216_class_03_00122_volume_sharp.mrc as #4, grid size |
| 11543 | | 128,128,128, pixel 2.08, shown at level 0.199, step 1, values float32 |
| 11544 | | |
| 11545 | | > open "/Users/peiyuyu/Documents/Goethe |
| 11546 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 11547 | | > F_B1/cryosparc_P4_J216_class_04_00122_volume_sharp.mrc" |
| 11548 | | |
| 11549 | | Opened cryosparc_P4_J216_class_04_00122_volume_sharp.mrc as #5, grid size |
| 11550 | | 128,128,128, pixel 2.08, shown at level 0.235, step 1, values float32 |
| 11551 | | |
| 11552 | | > lighting soft |
| 11553 | | |
| 11554 | | > set bgColor white |
| 11555 | | |
| 11556 | | > hide #!5 models |
| 11557 | | |
| 11558 | | > hide #!4 models |
| 11559 | | |
| 11560 | | > hide #!3 models |
| 11561 | | |
| 11562 | | > hide #!2 models |
| 11563 | | |
| 11564 | | > hide #!1 models |
| 11565 | | |
| 11566 | | > show #!5 models |
| 11567 | | |
| 11568 | | > hide #!5 models |
| 11569 | | |
| 11570 | | > show #!4 models |
| 11571 | | |
| 11572 | | > volume #4 level 0.2842 |
| 11573 | | |
| 11574 | | > show #!3 models |
| 11575 | | |
| 11576 | | > hide #!4 models |
| 11577 | | |
| 11578 | | > volume #3 level 0.3546 |
| 11579 | | |
| 11580 | | > volume #3 level 0.356 |
| 11581 | | |
| 11582 | | > hide #!3 models |
| 11583 | | |
| 11584 | | > show #!1 models |
| 11585 | | |
| 11586 | | > show #!2 models |
| 11587 | | |
| 11588 | | > hide #!2 models |
| 11589 | | |
| 11590 | | > show #!5 models |
| 11591 | | |
| 11592 | | > hide #!1 models |
| 11593 | | |
| 11594 | | > color #1 #FCEDFF |
| 11595 | | |
| 11596 | | > hide #!5 models |
| 11597 | | |
| 11598 | | > show #!1 models |
| 11599 | | |
| 11600 | | > show #!2 models |
| 11601 | | |
| 11602 | | > hide #!1 models |
| 11603 | | |
| 11604 | | > show #!3 models |
| 11605 | | |
| 11606 | | > hide #!3 models |
| 11607 | | |
| 11608 | | > show #!3 models |
| 11609 | | |
| 11610 | | > hide #!2 models |
| 11611 | | |
| 11612 | | > show #!4 models |
| 11613 | | |
| 11614 | | > hide #!3 models |
| 11615 | | |
| 11616 | | > hide #!4 models |
| 11617 | | |
| 11618 | | > show #!5 models |
| 11619 | | |
| 11620 | | > hide #!5 models |
| 11621 | | |
| 11622 | | > show #!1 models |
| 11623 | | |
| 11624 | | > volume flip #1 |
| 11625 | | |
| 11626 | | Opened cryosparc_P4_J216_class_00_00122_volume_sharp.mrc z flip as #6, grid |
| 11627 | | size 128,128,128, pixel 2.08, shown at step 1, values float32 |
| 11628 | | |
| 11629 | | > hide #!6 models |
| 11630 | | |
| 11631 | | > show #!2 models |
| 11632 | | |
| 11633 | | > volume flip #2 |
| 11634 | | |
| 11635 | | Opened cryosparc_P4_J216_class_01_00122_volume_sharp.mrc z flip as #7, grid |
| 11636 | | size 128,128,128, pixel 2.08, shown at step 1, values float32 |
| 11637 | | |
| 11638 | | > hide #!7 models |
| 11639 | | |
| 11640 | | > show #!1 models |
| 11641 | | |
| 11642 | | > hide #!1 models |
| 11643 | | |
| 11644 | | > show #!2 models |
| 11645 | | |
| 11646 | | > hide #!2 models |
| 11647 | | |
| 11648 | | > show #!3 models |
| 11649 | | |
| 11650 | | > hide #!3 models |
| 11651 | | |
| 11652 | | > volume flip #3 |
| 11653 | | |
| 11654 | | Opened cryosparc_P4_J216_class_02_00122_volume_sharp.mrc z flip as #8, grid |
| 11655 | | size 128,128,128, pixel 2.08, shown at step 1, values float32 |
| 11656 | | |
| 11657 | | > show #!4 models |
| 11658 | | |
| 11659 | | > hide #!4 models |
| 11660 | | |
| 11661 | | > show #!5 models |
| 11662 | | |
| 11663 | | > hide #!8 models |
| 11664 | | |
| 11665 | | > hide #!5 models |
| 11666 | | |
| 11667 | | > close #1 |
| 11668 | | |
| 11669 | | > close #2 |
| 11670 | | |
| 11671 | | > close #3 |
| 11672 | | |
| 11673 | | > rename #6 CS_P4_J216_class_0_sharp_flip id #1 |
| 11674 | | |
| 11675 | | > save #1 CS_P4_J216_class_0_sharp_flip.mrc |
| 11676 | | |
| 11677 | | Cannot determine format for '#1' |
| 11678 | | |
| 11679 | | > save CS_P4_J216_class_0_sharp_flip.mrc #1 |
| 11680 | | |
| 11681 | | > rename #7 CS_P4_J216_class_1_sharp_flip id #1_sharp_flip.mrc #2 |
| 11682 | | |
| 11683 | | Invalid "id" argument: Expected an integer |
| 11684 | | |
| 11685 | | > rename #7 CS_P4_J216_class_1_sharp_flip id #1_sharp_flip id #2 |
| 11686 | | |
| 11687 | | Invalid "id" argument: Expected an integer |
| 11688 | | |
| 11689 | | > rename #7 CS_P4_J216_class_2_sharp_flip id #2 |
| 11690 | | |
| 11691 | | > rename #2 CS_P4_J216_class_1_sharp_flip |
| 11692 | | |
| 11693 | | > rename #8 CS_P4_J216_class_2_sharp_flip id #3 |
| 11694 | | |
| 11695 | | > save CS_P4_J216_class_1_sharp_flip.mrc #2 |
| 11696 | | |
| 11697 | | > save CS_P4_J216_class_2_sharp_flip.mrc #3 |
| 11698 | | |
| 11699 | | > save "/Users/peiyuyu/Documents/Goethe |
| 11700 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 11701 | | > F_B1/20260430_SA-F_B1_workflow.cxs" |
| 11702 | | |
| 11703 | | > show #!1 models |
| 11704 | | |
| 11705 | | > show #!2 models |
| 11706 | | |
| 11707 | | > show #!3 models |
| 11708 | | |
| 11709 | | > show #!4 models |
| 11710 | | |
| 11711 | | > show #!5 models |
| 11712 | | |
| 11713 | | > hide #!4 models |
| 11714 | | |
| 11715 | | > hide #!3 models |
| 11716 | | |
| 11717 | | > hide #!2 models |
| 11718 | | |
| 11719 | | > hide #!1 models |
| 11720 | | |
| 11721 | | > show #!3 models |
| 11722 | | |
| 11723 | | > hide #!3 models |
| 11724 | | |
| 11725 | | > show #!4 models |
| 11726 | | |
| 11727 | | > hide #!5 models |
| 11728 | | |
| 11729 | | > hide #!4 models |
| 11730 | | |
| 11731 | | > show #!3 models |
| 11732 | | |
| 11733 | | > hide #!3 models |
| 11734 | | |
| 11735 | | > show #!2 models |
| 11736 | | |
| 11737 | | > hide #!2 models |
| 11738 | | |
| 11739 | | > show #!5 models |
| 11740 | | |
| 11741 | | > show #!3 models |
| 11742 | | |
| 11743 | | > hide #!5 models |
| 11744 | | |
| 11745 | | > show #!1 models |
| 11746 | | |
| 11747 | | > select add #1 |
| 11748 | | |
| 11749 | | 2 models selected |
| 11750 | | |
| 11751 | | > ui mousemode right "translate selected models" |
| 11752 | | |
| 11753 | | > view matrix models #1,1,0,0,-2.4868,0,1,0,6.8233,0,0,1,22.302 |
| 11754 | | |
| 11755 | | > ui mousemode right "rotate selected models" |
| 11756 | | |
| 11757 | | > view matrix models |
| 11758 | | > #1,0.85834,-0.51214,-0.03115,83.967,-0.50924,-0.85776,0.070198,298.29,-0.06267,-0.044391,-0.99705,270.86 |
| 11759 | | |
| 11760 | | Must specify one map, got 0 |
| 11761 | | |
| 11762 | | > fitmap #1 inMap #3 |
| 11763 | | |
| 11764 | | Fit map CS_P4_J216_class_0_sharp_flip.mrc in map |
| 11765 | | CS_P4_J216_class_2_sharp_flip.mrc using 20952 points |
| 11766 | | correlation = 0.8163, correlation about mean = 0.111, overlap = 1135 |
| 11767 | | steps = 116, shift = 13.1, angle = 14.8 degrees |
| 11768 | | |
| 11769 | | Position of CS_P4_J216_class_0_sharp_flip.mrc (#1) relative to |
| 11770 | | CS_P4_J216_class_2_sharp_flip.mrc (#3) coordinates: |
| 11771 | | Matrix rotation and translation |
| 11772 | | 0.94377261 -0.32509802 0.06003771 31.40521096 |
| 11773 | | -0.32212557 -0.94514622 -0.05416389 308.66342237 |
| 11774 | | 0.07435299 0.03177871 -0.99672551 251.62777867 |
| 11775 | | Axis 0.98583616 -0.16420866 0.03409658 |
| 11776 | | Axis point 0.00000000 154.16030965 128.66405805 |
| 11777 | | Rotation angle (degrees) 177.50176084 |
| 11778 | | Shift along axis -11.14516800 |
| 11779 | | |
| 11780 | | |
| 11781 | | > select subtract #1 |
| 11782 | | |
| 11783 | | Nothing selected |
| 11784 | | |
| 11785 | | > hide #!3 models |
| 11786 | | |
| 11787 | | > hide #!1 models |
| 11788 | | |
| 11789 | | > show #!2 models |
| 11790 | | |
| 11791 | | > show #!3 models |
| 11792 | | |
| 11793 | | > fitmap #2 inMap #3 |
| 11794 | | |
| 11795 | | Fit map CS_P4_J216_class_1_sharp_flip.mrc in map |
| 11796 | | CS_P4_J216_class_2_sharp_flip.mrc using 20964 points |
| 11797 | | correlation = 0.8775, correlation about mean = 0.2387, overlap = 1535 |
| 11798 | | steps = 72, shift = 7.04, angle = 9.35 degrees |
| 11799 | | |
| 11800 | | Position of CS_P4_J216_class_1_sharp_flip.mrc (#2) relative to |
| 11801 | | CS_P4_J216_class_2_sharp_flip.mrc (#3) coordinates: |
| 11802 | | Matrix rotation and translation |
| 11803 | | 0.99017956 0.04949629 -0.13074615 12.42570756 |
| 11804 | | -0.06022022 0.99502365 -0.07938169 18.18421737 |
| 11805 | | 0.12616642 0.08647569 0.98823276 -19.36289944 |
| 11806 | | Axis 0.51051058 -0.79077932 -0.33770846 |
| 11807 | | Axis point 165.72686650 0.00000000 88.43510724 |
| 11808 | | Rotation angle (degrees) 9.34870453 |
| 11809 | | Shift along axis -1.49723285 |
| 11810 | | |
| 11811 | | |
| 11812 | | > hide #!2 models |
| 11813 | | |
| 11814 | | > show #!4 models |
| 11815 | | |
| 11816 | | > fitmap #4 inMap #3 |
| 11817 | | |
| 11818 | | Fit map cryosparc_P4_J216_class_03_00122_volume_sharp.mrc in map |
| 11819 | | CS_P4_J216_class_2_sharp_flip.mrc using 5887 points |
| 11820 | | correlation = 0.7186, correlation about mean = 0.04573, overlap = 331.9 |
| 11821 | | steps = 104, shift = 11.1, angle = 13.1 degrees |
| 11822 | | |
| 11823 | | Position of cryosparc_P4_J216_class_03_00122_volume_sharp.mrc (#4) relative to |
| 11824 | | CS_P4_J216_class_2_sharp_flip.mrc (#3) coordinates: |
| 11825 | | Matrix rotation and translation |
| 11826 | | 0.97455290 -0.13512397 0.17885231 -1.53434267 |
| 11827 | | 0.14053006 0.98991548 -0.01785084 -17.74958210 |
| 11828 | | -0.17463660 0.04253071 0.98371398 30.92421506 |
| 11829 | | Axis 0.13349556 0.78151692 0.60943437 |
| 11830 | | Axis point 157.21397711 0.00000000 34.50744362 |
| 11831 | | Rotation angle (degrees) 13.07084305 |
| 11832 | | Shift along axis 4.76985279 |
| 11833 | | |
| 11834 | | |
| 11835 | | > hide #!4 models |
| 11836 | | |
| 11837 | | > show #!5 models |
| 11838 | | |
| 11839 | | > select add #5 |
| 11840 | | |
| 11841 | | 2 models selected |
| 11842 | | |
| 11843 | | > view matrix models |
| 11844 | | > #5,0.70232,-0.70945,-0.058532,142.48,-0.70661,-0.70474,0.063551,312.49,-0.086337,-0.0032735,-0.99626,292.78 |
| 11845 | | |
| 11846 | | > fitmap #5 inMap #3 |
| 11847 | | |
| 11848 | | Fit map cryosparc_P4_J216_class_04_00122_volume_sharp.mrc in map |
| 11849 | | CS_P4_J216_class_2_sharp_flip.mrc using 20952 points |
| 11850 | | correlation = 0.896, correlation about mean = 0.3255, overlap = 1903 |
| 11851 | | steps = 100, shift = 4.43, angle = 13 degrees |
| 11852 | | |
| 11853 | | Position of cryosparc_P4_J216_class_04_00122_volume_sharp.mrc (#5) relative to |
| 11854 | | CS_P4_J216_class_2_sharp_flip.mrc (#3) coordinates: |
| 11855 | | Matrix rotation and translation |
| 11856 | | 0.72688934 -0.68457986 0.05461051 119.19907428 |
| 11857 | | -0.67457364 -0.72664609 -0.13013783 337.91149541 |
| 11858 | | 0.12877226 0.05775699 -0.98999082 252.38058253 |
| 11859 | | Axis 0.92902840 -0.36668580 0.04947481 |
| 11860 | | Axis point 0.00000000 185.59312596 132.22855036 |
| 11861 | | Rotation angle (degrees) 174.19607958 |
| 11862 | | Shift along axis -0.68154259 |
| 11863 | | |
| 11864 | | |
| 11865 | | > select subtract #5 |
| 11866 | | |
| 11867 | | Nothing selected |
| 11868 | | |
| 11869 | | > save "/Users/peiyuyu/Documents/Goethe |
| 11870 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 11871 | | > F_B1/20260430_SA-F_B1_workflow.cxs" |
| 11872 | | |
| 11873 | | > hide #!5 models |
| 11874 | | |
| 11875 | | > show #!5 models |
| 11876 | | |
| 11877 | | > hide #!3 models |
| 11878 | | |
| 11879 | | > show #!1 models |
| 11880 | | |
| 11881 | | > hide #!5 models |
| 11882 | | |
| 11883 | | > hide #!1 models |
| 11884 | | |
| 11885 | | > show #!2 models |
| 11886 | | |
| 11887 | | > color #2 #FCEDFF |
| 11888 | | |
| 11889 | | > hide #!2 models |
| 11890 | | |
| 11891 | | > show #!4 models |
| 11892 | | |
| 11893 | | > color #4 #FCEDFF |
| 11894 | | |
| 11895 | | > hide #!4 models |
| 11896 | | |
| 11897 | | > show #!5 models |
| 11898 | | |
| 11899 | | > color #5 #FCEDFF |
| 11900 | | |
| 11901 | | > hide #!5 models |
| 11902 | | |
| 11903 | | > show #!3 models |
| 11904 | | |
| 11905 | | > color #5 #E9C29B |
| 11906 | | |
| 11907 | | > color #5 #FCEDFF |
| 11908 | | |
| 11909 | | > color #3 #E9C29B |
| 11910 | | |
| 11911 | | > ui mousemode right translate |
| 11912 | | |
| 11913 | | > volume #2 level 0.356 |
| 11914 | | |
| 11915 | | > volume #1 level 0.356 |
| 11916 | | |
| 11917 | | > volume #4 level 0.356 |
| 11918 | | |
| 11919 | | > volume #5 level 0.356 |
| 11920 | | |
| 11921 | | > hide #!3 models |
| 11922 | | |
| 11923 | | > show #!1 models |
| 11924 | | |
| 11925 | | > undo |
| 11926 | | |
| 11927 | | [Repeated 1 time(s)] |
| 11928 | | |
| 11929 | | > hide #!3 models |
| 11930 | | |
| 11931 | | > show #!1 models |
| 11932 | | |
| 11933 | | > hide #!1 models |
| 11934 | | |
| 11935 | | > show #!1 models |
| 11936 | | |
| 11937 | | > hide #!1 models |
| 11938 | | |
| 11939 | | > show #!1 models |
| 11940 | | |
| 11941 | | > hide #!1 models |
| 11942 | | |
| 11943 | | > show #!1 models |
| 11944 | | |
| 11945 | | > hide #!1 models |
| 11946 | | |
| 11947 | | > show #!1 models |
| 11948 | | |
| 11949 | | > volume #1 level 0.1727 |
| 11950 | | |
| 11951 | | > volume #1 level 0.1851 |
| 11952 | | |
| 11953 | | > show #!2 models |
| 11954 | | |
| 11955 | | > hide #!1 models |
| 11956 | | |
| 11957 | | > volume #2 level 0.203 |
| 11958 | | |
| 11959 | | > show #!3 models |
| 11960 | | |
| 11961 | | > hide #!2 models |
| 11962 | | |
| 11963 | | > hide #!3 models |
| 11964 | | |
| 11965 | | > show #!4 models |
| 11966 | | |
| 11967 | | > volume #4 level 0.1532 |
| 11968 | | |
| 11969 | | > show #!3 models |
| 11970 | | |
| 11971 | | > select add #4 |
| 11972 | | |
| 11973 | | 2 models selected |
| 11974 | | |
| 11975 | | > ui mousemode right "translate selected models" |
| 11976 | | |
| 11977 | | > view matrix models |
| 11978 | | > #4,0.97455,-0.13512,0.17885,1.3208,0.14053,0.98992,-0.017851,-10.868,-0.17464,0.042531,0.98371,31.337 |
| 11979 | | |
| 11980 | | > ui mousemode right "rotate selected models" |
| 11981 | | |
| 11982 | | > view matrix models |
| 11983 | | > #4,-0.085498,-0.64922,0.75578,120.23,0.99605,-0.074063,0.049058,8.8958,0.024125,0.75698,0.65299,-39.323 |
| 11984 | | |
| 11985 | | > ui mousemode right "rotate selected models" |
| 11986 | | |
| 11987 | | > ui mousemode right "translate selected models" |
| 11988 | | |
| 11989 | | > view matrix models |
| 11990 | | > #4,-0.085498,-0.64922,0.75578,134.23,0.99605,-0.074063,0.049058,6.1939,0.024125,0.75698,0.65299,-87.477 |
| 11991 | | |
| 11992 | | > view matrix models |
| 11993 | | > #4,-0.085498,-0.64922,0.75578,134.33,0.99605,-0.074063,0.049058,6.22,0.024125,0.75698,0.65299,-87.742 |
| 11994 | | |
| 11995 | | > view matrix models |
| 11996 | | > #4,-0.085498,-0.64922,0.75578,130.4,0.99605,-0.074063,0.049058,5.0583,0.024125,0.75698,0.65299,-76.883 |
| 11997 | | |
| 11998 | | > fitmap #4 inMap #3 |
| 11999 | | |
| 12000 | | Fit map cryosparc_P4_J216_class_03_00122_volume_sharp.mrc in map |
| 12001 | | CS_P4_J216_class_2_sharp_flip.mrc using 42435 points |
| 12002 | | correlation = 0.7796, correlation about mean = 0.1614, overlap = 1558 |
| 12003 | | steps = 228, shift = 2.07, angle = 23.4 degrees |
| 12004 | | |
| 12005 | | Position of cryosparc_P4_J216_class_03_00122_volume_sharp.mrc (#4) relative to |
| 12006 | | CS_P4_J216_class_2_sharp_flip.mrc (#3) coordinates: |
| 12007 | | Matrix rotation and translation |
| 12008 | | -0.31733316 -0.35582789 0.87902570 104.58434579 |
| 12009 | | 0.93180439 -0.28920783 0.21931579 18.83015631 |
| 12010 | | 0.17618244 0.88867618 0.42333722 -81.48445272 |
| 12011 | | Axis 0.41511738 0.43588247 0.79855121 |
| 12012 | | Axis point 65.64014873 66.59534740 0.00000000 |
| 12013 | | Rotation angle (degrees) 126.27076538 |
| 12014 | | Shift along axis -13.44699307 |
| 12015 | | |
| 12016 | | |
| 12017 | | > ui mousemode right "rotate selected models" |
| 12018 | | |
| 12019 | | > view matrix models |
| 12020 | | > #4,-0.85396,0.083596,-0.51358,313.46,-0.52034,-0.13698,0.8429,94.578,0.00011363,0.98704,0.16047,-34.579 |
| 12021 | | |
| 12022 | | > fitmap #4 inMap #3 |
| 12023 | | |
| 12024 | | Fit map cryosparc_P4_J216_class_03_00122_volume_sharp.mrc in map |
| 12025 | | CS_P4_J216_class_2_sharp_flip.mrc using 42435 points |
| 12026 | | correlation = 0.7628, correlation about mean = 0.1138, overlap = 1507 |
| 12027 | | steps = 160, shift = 8.1, angle = 11.6 degrees |
| 12028 | | |
| 12029 | | Position of cryosparc_P4_J216_class_03_00122_volume_sharp.mrc (#4) relative to |
| 12030 | | CS_P4_J216_class_2_sharp_flip.mrc (#3) coordinates: |
| 12031 | | Matrix rotation and translation |
| 12032 | | -0.75756109 0.20637267 -0.61928307 295.50473254 |
| 12033 | | -0.63935005 -0.04324082 0.76769900 115.47474727 |
| 12034 | | 0.13165378 0.97751755 0.16470194 -51.62941296 |
| 12035 | | Axis 0.18240424 -0.65282152 -0.73522293 |
| 12036 | | Axis point 168.50534676 17.73009752 0.00000000 |
| 12037 | | Rotation angle (degrees) 144.89006320 |
| 12038 | | Shift along axis 16.47604383 |
| 12039 | | |
| 12040 | | |
| 12041 | | > select subtract #4 |
| 12042 | | |
| 12043 | | Nothing selected |
| 12044 | | |
| 12045 | | > volume #4 level 0.3211 |
| 12046 | | |
| 12047 | | > hide #!3 models |
| 12048 | | |
| 12049 | | > volume #4 level 0.2347 |
| 12050 | | |
| 12051 | | > volume #4 level 0.2031 |
| 12052 | | |
| 12053 | | > hide #!4 models |
| 12054 | | |
| 12055 | | > show #!5 models |
| 12056 | | |
| 12057 | | > volume #5 level 0.1924 |
| 12058 | | |
| 12059 | | > volume #5 level 0.2064 |
| 12060 | | |
| 12061 | | > ui mousemode right translate |
| 12062 | | |
| 12063 | | > show #!3 models |
| 12064 | | |
| 12065 | | > hide #!5 models |
| 12066 | | |
| 12067 | | > color #3 #B9DBD5 |
| 12068 | | |
| 12069 | | > hide #!3 models |
| 12070 | | |
| 12071 | | > show #!1 models |
| 12072 | | |
| 12073 | | > lighting shadows true intensity 0.5 |
| 12074 | | |
| 12075 | | > lighting flat |
| 12076 | | |
| 12077 | | > graphics silhouettes false |
| 12078 | | |
| 12079 | | > graphics silhouettes true |
| 12080 | | |
| 12081 | | > lighting soft |
| 12082 | | |
| 12083 | | > save P4_J216_class_0_sharp.png supersample 3 transparentBackground true |
| 12084 | | |
| 12085 | | > hide #!1 models |
| 12086 | | |
| 12087 | | > show #!2 models |
| 12088 | | |
| 12089 | | > save P4_J216_class_1_sharp.png supersample 3 transparentBackground true |
| 12090 | | |
| 12091 | | > hide #!2 models |
| 12092 | | |
| 12093 | | > show #!3 models |
| 12094 | | |
| 12095 | | > save P4_J216_class_2_sharp.png supersample 3 transparentBackground true |
| 12096 | | |
| 12097 | | > hide #!3 models |
| 12098 | | |
| 12099 | | > show #!4 models |
| 12100 | | |
| 12101 | | > save P4_J216_class_3_sharp.png supersample 3 transparentBackground true |
| 12102 | | |
| 12103 | | > hide #!4 models |
| 12104 | | |
| 12105 | | > show #!5 models |
| 12106 | | |
| 12107 | | > show #!1 models |
| 12108 | | |
| 12109 | | > show #!2 models |
| 12110 | | |
| 12111 | | > show #!3 models |
| 12112 | | |
| 12113 | | > show #!4 models |
| 12114 | | |
| 12115 | | > hide #!4 models |
| 12116 | | |
| 12117 | | > hide #!5 models |
| 12118 | | |
| 12119 | | > show #!5 models |
| 12120 | | |
| 12121 | | > hide #!5 models |
| 12122 | | |
| 12123 | | > hide #!3 models |
| 12124 | | |
| 12125 | | > hide #!2 models |
| 12126 | | |
| 12127 | | > hide #!1 models |
| 12128 | | |
| 12129 | | > show #!1 models |
| 12130 | | |
| 12131 | | > view name P1 |
| 12132 | | |
| 12133 | | [Repeated 1 time(s)] |
| 12134 | | |
| 12135 | | > save P4_J216_class_0_sharp.png supersample 3 transparentBackground true |
| 12136 | | |
| 12137 | | > hide #!1 models |
| 12138 | | |
| 12139 | | > show #!2 models |
| 12140 | | |
| 12141 | | > save P4_J216_class_1_sharp.png supersample 3 transparentBackground true |
| 12142 | | |
| 12143 | | > hide #!2 models |
| 12144 | | |
| 12145 | | > show #!3 models |
| 12146 | | |
| 12147 | | > hide #!3 models |
| 12148 | | |
| 12149 | | > show #!2 models |
| 12150 | | |
| 12151 | | > hide #!2 models |
| 12152 | | |
| 12153 | | > show #!1 models |
| 12154 | | |
| 12155 | | > hide #!1 models |
| 12156 | | |
| 12157 | | > show #!2 models |
| 12158 | | |
| 12159 | | > hide #!2 models |
| 12160 | | |
| 12161 | | > show #!3 models |
| 12162 | | |
| 12163 | | > save P4_J216_class_2_sharp.png supersample 3 transparentBackground true |
| 12164 | | |
| 12165 | | > hide #!3 models |
| 12166 | | |
| 12167 | | > show #!4 models |
| 12168 | | |
| 12169 | | > hide #!4 models |
| 12170 | | |
| 12171 | | > show #!5 models |
| 12172 | | |
| 12173 | | > hide #!5 models |
| 12174 | | |
| 12175 | | > show #!4 models |
| 12176 | | |
| 12177 | | > save P4_J216_class_3_sharp.png supersample 3 transparentBackground true |
| 12178 | | |
| 12179 | | > hide #!4 models |
| 12180 | | |
| 12181 | | > show #!5 models |
| 12182 | | |
| 12183 | | > vieww P1 |
| 12184 | | |
| 12185 | | Unknown command: vieww P1 |
| 12186 | | |
| 12187 | | > view P1 |
| 12188 | | |
| 12189 | | [Repeated 1 time(s)] |
| 12190 | | |
| 12191 | | > save P4_J216_class_4_sharp.png supersample 3 transparentBackground true |
| 12192 | | |
| 12193 | | > save "/Users/peiyuyu/Documents/Goethe |
| 12194 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 12195 | | > F_B1/20260430_SA-F_B1_workflow.cxs" |
| 12196 | | |
| 12197 | | > open "/Users/peiyuyu/Documents/Goethe |
| 12198 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 12199 | | > F_B1/cryosparc_P4_J219_002_volume_map_sharp.mrc" |
| 12200 | | |
| 12201 | | Opened cryosparc_P4_J219_002_volume_map_sharp.mrc as #6, grid size 80,80,80, |
| 12202 | | pixel 3.32, shown at level 0.854, step 1, values float32 |
| 12203 | | |
| 12204 | | > hide #!5 models |
| 12205 | | |
| 12206 | | > show #!3 models |
| 12207 | | |
| 12208 | | > select add #6 |
| 12209 | | |
| 12210 | | 2 models selected |
| 12211 | | |
| 12212 | | > ui mousemode right "rotate selected models" |
| 12213 | | |
| 12214 | | > view matrix models |
| 12215 | | > #6,0.50968,-0.8597,0.033732,182.49,-0.85693,-0.50376,0.10903,313.05,-0.076743,-0.084479,-0.99347,263.76 |
| 12216 | | |
| 12217 | | > ui mousemode right "translate selected models" |
| 12218 | | |
| 12219 | | > view matrix models |
| 12220 | | > #6,0.50968,-0.8597,0.033732,181.37,-0.85693,-0.50376,0.10903,307.2,-0.076743,-0.084479,-0.99347,286.3 |
| 12221 | | |
| 12222 | | > fitmap #6 inMap #3 |
| 12223 | | |
| 12224 | | Fit map cryosparc_P4_J219_002_volume_map_sharp.mrc in map |
| 12225 | | CS_P4_J216_class_2_sharp_flip.mrc using 5120 points |
| 12226 | | correlation = 0.7368, correlation about mean = 0.07448, overlap = 1741 |
| 12227 | | steps = 88, shift = 8.42, angle = 12.4 degrees |
| 12228 | | |
| 12229 | | Position of cryosparc_P4_J219_002_volume_map_sharp.mrc (#6) relative to |
| 12230 | | CS_P4_J216_class_2_sharp_flip.mrc (#3) coordinates: |
| 12231 | | Matrix rotation and translation |
| 12232 | | 0.68179907 -0.73031354 0.04233404 134.55628275 |
| 12233 | | -0.72995927 -0.67538450 0.10495351 308.10402853 |
| 12234 | | -0.04805721 -0.10245933 -0.99357566 283.32032359 |
| 12235 | | Axis -0.91672636 0.39951261 0.00156579 |
| 12236 | | Axis point 0.00000000 192.14085888 132.24148806 |
| 12237 | | Rotation angle (degrees) 173.50439997 |
| 12238 | | Shift along axis 0.18377410 |
| 12239 | | |
| 12240 | | |
| 12241 | | > volume #6 level 1.828 |
| 12242 | | |
| 12243 | | > select subtract #6 |
| 12244 | | |
| 12245 | | Nothing selected |
| 12246 | | |
| 12247 | | > hide #!3 models |
| 12248 | | |
| 12249 | | > volume #6 level 1.172 |
| 12250 | | |
| 12251 | | > show #!3 models |
| 12252 | | |
| 12253 | | > select add #6 |
| 12254 | | |
| 12255 | | 2 models selected |
| 12256 | | |
| 12257 | | > view matrix models |
| 12258 | | > #6,0.6818,-0.73031,0.042334,145.51,-0.72996,-0.67538,0.10495,294.75,-0.048057,-0.10246,-0.99358,285.84 |
| 12259 | | |
| 12260 | | > view matrix models |
| 12261 | | > #6,0.6818,-0.73031,0.042334,133.79,-0.72996,-0.67538,0.10495,309.04,-0.048057,-0.10246,-0.99358,282.76 |
| 12262 | | |
| 12263 | | > ui mousemode right "rotate selected models" |
| 12264 | | |
| 12265 | | > view matrix models |
| 12266 | | > #6,0.47143,0.87224,0.13024,-67.58,0.87592,-0.44591,-0.18423,88.863,-0.10262,0.20093,-0.97422,246.36 |
| 12267 | | |
| 12268 | | > fitmap #6 inMap #3 |
| 12269 | | |
| 12270 | | Fit map cryosparc_P4_J219_002_volume_map_sharp.mrc in map |
| 12271 | | CS_P4_J216_class_2_sharp_flip.mrc using 3507 points |
| 12272 | | correlation = 0.7523, correlation about mean = 0.053, overlap = 1436 |
| 12273 | | steps = 84, shift = 11, angle = 9.96 degrees |
| 12274 | | |
| 12275 | | Position of cryosparc_P4_J219_002_volume_map_sharp.mrc (#6) relative to |
| 12276 | | CS_P4_J216_class_2_sharp_flip.mrc (#3) coordinates: |
| 12277 | | Matrix rotation and translation |
| 12278 | | 0.31271016 0.94213099 0.12083688 -48.05138587 |
| 12279 | | 0.94223385 -0.29160641 -0.16480619 65.68302492 |
| 12280 | | -0.12003221 0.16539317 -0.97889599 255.74245569 |
| 12281 | | Axis 0.80789250 0.58932983 0.00025166 |
| 12282 | | Axis point 0.00000000 34.02227920 132.07845223 |
| 12283 | | Rotation angle (degrees) 168.20805262 |
| 12284 | | Shift along axis -0.04702893 |
| 12285 | | |
| 12286 | | |
| 12287 | | > select subtract #6 |
| 12288 | | |
| 12289 | | Nothing selected |
| 12290 | | |
| 12291 | | > hide #!6 models |
| 12292 | | |
| 12293 | | > show #!6 models |
| 12294 | | |
| 12295 | | > select add #6 |
| 12296 | | |
| 12297 | | 2 models selected |
| 12298 | | |
| 12299 | | > view matrix models |
| 12300 | | > #6,-0.96774,-0.18791,0.16785,278.55,-0.18769,0.98208,0.017294,25.636,-0.16809,-0.014768,-0.98566,287.91 |
| 12301 | | |
| 12302 | | > ui mousemode right "translate selected models" |
| 12303 | | |
| 12304 | | > view matrix models |
| 12305 | | > #6,-0.96774,-0.18791,0.16785,272.6,-0.18769,0.98208,0.017294,32.876,-0.16809,-0.014768,-0.98566,284.26 |
| 12306 | | |
| 12307 | | > fitmap #6 inMap #3 |
| 12308 | | |
| 12309 | | Fit map cryosparc_P4_J219_002_volume_map_sharp.mrc in map |
| 12310 | | CS_P4_J216_class_2_sharp_flip.mrc using 3507 points |
| 12311 | | correlation = 0.7248, correlation about mean = 0.05418, overlap = 1264 |
| 12312 | | steps = 60, shift = 9.72, angle = 10.6 degrees |
| 12313 | | |
| 12314 | | Position of cryosparc_P4_J219_002_volume_map_sharp.mrc (#6) relative to |
| 12315 | | CS_P4_J216_class_2_sharp_flip.mrc (#3) coordinates: |
| 12316 | | Matrix rotation and translation |
| 12317 | | -0.92647017 -0.30027989 0.22690311 266.55888821 |
| 12318 | | -0.26721766 0.94935570 0.16528304 28.41095713 |
| 12319 | | -0.26504293 0.09249729 -0.95978982 282.00607809 |
| 12320 | | Axis -0.14603906 0.98705219 0.06633684 |
| 12321 | | Axis point 153.43261550 0.00000000 122.88099835 |
| 12322 | | Rotation angle (degrees) 165.56984584 |
| 12323 | | Shift along axis 7.82248126 |
| 12324 | | |
| 12325 | | |
| 12326 | | > ui mousemode right "rotate selected models" |
| 12327 | | |
| 12328 | | > view matrix models |
| 12329 | | > #6,-0.92785,-0.005043,0.37292,209.61,0.027742,0.99621,0.082497,-9.3167,-0.37192,0.086891,-0.92419,293.48 |
| 12330 | | |
| 12331 | | > fitmap #6 inMap #3 |
| 12332 | | |
| 12333 | | Fit map cryosparc_P4_J219_002_volume_map_sharp.mrc in map |
| 12334 | | CS_P4_J216_class_2_sharp_flip.mrc using 3507 points |
| 12335 | | correlation = 0.7519, correlation about mean = 0.05711, overlap = 1436 |
| 12336 | | steps = 100, shift = 5.47, angle = 14.5 degrees |
| 12337 | | |
| 12338 | | Position of cryosparc_P4_J219_002_volume_map_sharp.mrc (#6) relative to |
| 12339 | | CS_P4_J216_class_2_sharp_flip.mrc (#3) coordinates: |
| 12340 | | Matrix rotation and translation |
| 12341 | | -0.92643320 -0.21423055 0.30955905 249.03084383 |
| 12342 | | -0.21368345 0.97623560 0.03610312 27.35482068 |
| 12343 | | -0.30993696 -0.03270052 -0.95019459 303.95121642 |
| 12344 | | Axis -0.11038513 0.99388850 0.00087774 |
| 12345 | | Axis point 150.47041621 0.00000000 131.94319456 |
| 12346 | | Rotation angle (degrees) 161.84114382 |
| 12347 | | Shift along axis -0.03486954 |
| 12348 | | |
| 12349 | | |
| 12350 | | > select subtract #6 |
| 12351 | | |
| 12352 | | Nothing selected |
| 12353 | | |
| 12354 | | > hide #!3 models |
| 12355 | | |
| 12356 | | > hide #!6 models |
| 12357 | | |
| 12358 | | > show #!3 models |
| 12359 | | |
| 12360 | | > hide #!3 models |
| 12361 | | |
| 12362 | | > show #!6 models |
| 12363 | | |
| 12364 | | > volume flip #6 |
| 12365 | | |
| 12366 | | Opened cryosparc_P4_J219_002_volume_map_sharp.mrc z flip as #7, grid size |
| 12367 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 12368 | | |
| 12369 | | > close #6 |
| 12370 | | |
| 12371 | | > rename CS_P4_J219_sharp_flip id #6 |
| 12372 | | |
| 12373 | | Missing or invalid "models" argument: invalid models specifier |
| 12374 | | |
| 12375 | | > rename #7 CS_P4_J219_sharp_flip id #6 |
| 12376 | | |
| 12377 | | > save CS_P4_J219_sharp_flip.mrc #6 |
| 12378 | | |
| 12379 | | > show #!3 models |
| 12380 | | |
| 12381 | | > select add #6 |
| 12382 | | |
| 12383 | | 2 models selected |
| 12384 | | |
| 12385 | | > view matrix models |
| 12386 | | > #6,0.55385,-0.83087,-0.054,184.1,0.82508,0.55638,-0.098423,-37.993,0.11182,0.009957,0.99368,-50.391 |
| 12387 | | |
| 12388 | | > ui mousemode right "translate selected models" |
| 12389 | | |
| 12390 | | > view matrix models |
| 12391 | | > #6,0.55385,-0.83087,-0.054,173.54,0.82508,0.55638,-0.098423,-43.189,0.11182,0.009957,0.99368,-15.893 |
| 12392 | | |
| 12393 | | > ui mousemode right "rotate selected models" |
| 12394 | | |
| 12395 | | > view matrix models |
| 12396 | | > #6,-0.43591,-0.88398,-0.16899,334.46,0.87276,-0.46105,0.1604,51.515,-0.2197,-0.077569,0.97248,45.011 |
| 12397 | | |
| 12398 | | > fitmap #6 inMap #3 |
| 12399 | | |
| 12400 | | Fit map CS_P4_J219_sharp_flip.mrc in map CS_P4_J216_class_2_sharp_flip.mrc |
| 12401 | | using 3507 points |
| 12402 | | correlation = 0.9414, correlation about mean = 0.4694, overlap = 2583 |
| 12403 | | steps = 72, shift = 3.53, angle = 4.05 degrees |
| 12404 | | |
| 12405 | | Position of CS_P4_J219_sharp_flip.mrc (#6) relative to |
| 12406 | | CS_P4_J216_class_2_sharp_flip.mrc (#3) coordinates: |
| 12407 | | Matrix rotation and translation |
| 12408 | | -0.45821390 -0.85833568 -0.23086770 342.48607306 |
| 12409 | | 0.85407817 -0.49711214 0.15306861 63.33623820 |
| 12410 | | -0.24615139 -0.12704090 0.96086946 56.91191433 |
| 12411 | | Axis -0.16142412 0.00880783 0.98684582 |
| 12412 | | Axis point 157.30567711 132.03749632 0.00000000 |
| 12413 | | Rotation angle (degrees) 119.81679395 |
| 12414 | | Shift along axis 1.43562700 |
| 12415 | | |
| 12416 | | |
| 12417 | | > select subtract #6 |
| 12418 | | |
| 12419 | | Nothing selected |
| 12420 | | |
| 12421 | | > hide #!3 models |
| 12422 | | |
| 12423 | | Unsupported scale factor (0.000000) detected on Display1 |
| 12424 | | |
| 12425 | | [Repeated 2 time(s)] |
| 12426 | | |
| 12427 | | > hide #!6 models |
| 12428 | | |
| 12429 | | > show #!3 models |
| 12430 | | |
| 12431 | | > show #!6 models |
| 12432 | | |
| 12433 | | > hide #!6 models |
| 12434 | | |
| 12435 | | > color #3 #B1DCFC |
| 12436 | | |
| 12437 | | > save P4_J216_class_2_sharp.png supersample 3 transparentBackground true |
| 12438 | | |
| 12439 | | > hide #!3 models |
| 12440 | | |
| 12441 | | > show #!3 models |
| 12442 | | |
| 12443 | | > hide #!3 models |
| 12444 | | |
| 12445 | | > show #!6 models |
| 12446 | | |
| 12447 | | > hide #!6 models |
| 12448 | | |
| 12449 | | > show #!3 models |
| 12450 | | |
| 12451 | | > show #!6 models |
| 12452 | | |
| 12453 | | > hide #!3 models |
| 12454 | | |
| 12455 | | > hide #!6 models |
| 12456 | | |
| 12457 | | > show #!3 models |
| 12458 | | |
| 12459 | | > color #3 #AFDBFF |
| 12460 | | |
| 12461 | | > hide #!3 models |
| 12462 | | |
| 12463 | | > show #!6 models |
| 12464 | | |
| 12465 | | > show #!3 models |
| 12466 | | |
| 12467 | | > hide #!3 models |
| 12468 | | |
| 12469 | | > show #!3 models |
| 12470 | | |
| 12471 | | > hide #!6 models |
| 12472 | | |
| 12473 | | > show #!6 models |
| 12474 | | |
| 12475 | | > color #3 #99BFE5 |
| 12476 | | |
| 12477 | | > hide #!6 models |
| 12478 | | |
| 12479 | | > view P1 |
| 12480 | | |
| 12481 | | > save P4_J216_class_2_sharp.png supersample 3 transparentBackground true |
| 12482 | | |
| 12483 | | > hide #!3 models |
| 12484 | | |
| 12485 | | > show #!6 models |
| 12486 | | |
| 12487 | | > ui mousemode right translate |
| 12488 | | |
| 12489 | | > save P4_J219_NU_sharp_flip.png supersample 3 transparentBackground true |
| 12490 | | |
| 12491 | | > save "/Users/peiyuyu/Documents/Goethe |
| 12492 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 12493 | | > F_B1/20260430_SA-F_B1_workflow.cxs" |
| 12494 | | |
| 12495 | | > open "/Users/peiyuyu/Documents/Goethe |
| 12496 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 12497 | | > F_B1/cryosparc_P4_J243_003_volume_map_sharp.mrc" |
| 12498 | | |
| 12499 | | Opened cryosparc_P4_J243_003_volume_map_sharp.mrc as #7, grid size 80,80,80, |
| 12500 | | pixel 3.32, shown at level 1.1, step 1, values float32 |
| 12501 | | |
| 12502 | | > select add #7 |
| 12503 | | |
| 12504 | | 2 models selected |
| 12505 | | |
| 12506 | | > ui mousemode right "rotate selected models" |
| 12507 | | |
| 12508 | | > view matrix models |
| 12509 | | > #7,0.34351,0.88804,0.30558,-69.154,0.93106,-0.36463,0.013018,61.425,0.12299,0.28005,-0.95208,168.53 |
| 12510 | | |
| 12511 | | > view matrix models |
| 12512 | | > #7,-0.72211,0.66266,0.19859,108.59,0.68148,0.73076,0.039546,-55.441,-0.11892,0.16389,-0.97928,217.85 |
| 12513 | | |
| 12514 | | > ui mousemode right "translate selected models" |
| 12515 | | |
| 12516 | | > view matrix models |
| 12517 | | > #7,-0.72211,0.66266,0.19859,107.8,0.68148,0.73076,0.039546,-63.119,-0.11892,0.16389,-0.97928,255.33 |
| 12518 | | |
| 12519 | | > view matrix models |
| 12520 | | > #7,-0.72211,0.66266,0.19859,116.79,0.68148,0.73076,0.039546,-56.849,-0.11892,0.16389,-0.97928,258.31 |
| 12521 | | |
| 12522 | | > fitmap #7 inMap #6 |
| 12523 | | |
| 12524 | | Fit map cryosparc_P4_J243_003_volume_map_sharp.mrc in map |
| 12525 | | CS_P4_J219_sharp_flip.mrc using 5116 points |
| 12526 | | correlation = 0.9377, correlation about mean = 0.7097, overlap = 1.519e+04 |
| 12527 | | steps = 76, shift = 2.16, angle = 10.6 degrees |
| 12528 | | |
| 12529 | | Position of cryosparc_P4_J243_003_volume_map_sharp.mrc (#7) relative to |
| 12530 | | CS_P4_J219_sharp_flip.mrc (#6) coordinates: |
| 12531 | | Matrix rotation and translation |
| 12532 | | 0.96909604 0.16642828 0.18208375 -39.18937125 |
| 12533 | | 0.15922720 -0.98578684 0.05358170 236.77616183 |
| 12534 | | 0.18841328 -0.02293313 -0.98182203 241.04145354 |
| 12535 | | Axis -0.99224103 -0.08208102 -0.09338329 |
| 12536 | | Axis point 0.00000000 122.34870623 120.06626952 |
| 12537 | | Rotation angle (degrees) 177.79032322 |
| 12538 | | Shift along axis -3.05877231 |
| 12539 | | |
| 12540 | | |
| 12541 | | > select subtract #7 |
| 12542 | | |
| 12543 | | Nothing selected |
| 12544 | | |
| 12545 | | > select add #7 |
| 12546 | | |
| 12547 | | 2 models selected |
| 12548 | | |
| 12549 | | > view matrix models |
| 12550 | | > #7,-0.62422,0.77517,0.097247,102.65,0.77737,0.62868,-0.021408,-52.876,-0.077732,0.062233,-0.99503,269.09 |
| 12551 | | |
| 12552 | | > ui mousemode right "rotate selected models" |
| 12553 | | |
| 12554 | | > view matrix models |
| 12555 | | > #7,-0.32669,-0.93485,0.13901,287.37,-0.94032,0.30667,-0.14745,222.88,0.095218,-0.17888,-0.97925,277.34 |
| 12556 | | |
| 12557 | | > ui mousemode right "translate selected models" |
| 12558 | | |
| 12559 | | > view matrix models |
| 12560 | | > #7,-0.32669,-0.93485,0.13901,281.61,-0.94032,0.30667,-0.14745,233.21,0.095218,-0.17888,-0.97925,273.65 |
| 12561 | | |
| 12562 | | > fitmap #7 inMap #6 |
| 12563 | | |
| 12564 | | Fit map cryosparc_P4_J243_003_volume_map_sharp.mrc in map |
| 12565 | | CS_P4_J219_sharp_flip.mrc using 5116 points |
| 12566 | | correlation = 0.9419, correlation about mean = 0.7222, overlap = 1.551e+04 |
| 12567 | | steps = 56, shift = 3.52, angle = 7.25 degrees |
| 12568 | | |
| 12569 | | Position of cryosparc_P4_J243_003_volume_map_sharp.mrc (#7) relative to |
| 12570 | | CS_P4_J219_sharp_flip.mrc (#6) coordinates: |
| 12571 | | Matrix rotation and translation |
| 12572 | | -0.62645631 0.77281267 0.10155321 101.66723571 |
| 12573 | | 0.77509514 0.63140454 -0.02357570 -50.90352474 |
| 12574 | | -0.08234076 0.06394425 -0.99455072 266.97848709 |
| 12575 | | Axis 0.42971205 0.90289643 0.01120665 |
| 12576 | | Axis point 70.49413903 0.00000000 130.93970837 |
| 12577 | | Rotation angle (degrees) 174.15511884 |
| 12578 | | Shift along axis 0.71895896 |
| 12579 | | |
| 12580 | | |
| 12581 | | > select subtract #7 |
| 12582 | | |
| 12583 | | Nothing selected |
| 12584 | | |
| 12585 | | > hide #!6 models |
| 12586 | | |
| 12587 | | > volume #7 level 1.41 |
| 12588 | | |
| 12589 | | > color #7 #99BFE5 |
| 12590 | | |
| 12591 | | > view P1 |
| 12592 | | |
| 12593 | | > close #7 |
| 12594 | | |
| 12595 | | > open "/Users/peiyuyu/Documents/Goethe |
| 12596 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 12597 | | > F_B1/cryosparc_P4_J244_003_volume_map_sharp.mrc" |
| 12598 | | |
| 12599 | | Opened cryosparc_P4_J244_003_volume_map_sharp.mrc as #7, grid size 80,80,80, |
| 12600 | | pixel 3.32, shown at level 1.05, step 1, values float32 |
| 12601 | | |
| 12602 | | > show #!6 models |
| 12603 | | |
| 12604 | | > select add #7 |
| 12605 | | |
| 12606 | | 2 models selected |
| 12607 | | |
| 12608 | | > ui mousemode right "rotate selected models" |
| 12609 | | |
| 12610 | | > view matrix models |
| 12611 | | > #7,0.71966,-0.6805,-0.13788,149.49,-0.69285,-0.71678,-0.078638,342.79,-0.045318,0.15212,-0.98732,223.99 |
| 12612 | | |
| 12613 | | > hide #!6 models |
| 12614 | | |
| 12615 | | > show #!6 models |
| 12616 | | |
| 12617 | | > ui mousemode right "translate selected models" |
| 12618 | | |
| 12619 | | > view matrix models |
| 12620 | | > #7,0.71966,-0.6805,-0.13788,149.4,-0.69285,-0.71678,-0.078638,338.98,-0.045318,0.15212,-0.98732,258.19 |
| 12621 | | |
| 12622 | | > select subtract #7 |
| 12623 | | |
| 12624 | | Nothing selected |
| 12625 | | |
| 12626 | | > hide #!6 models |
| 12627 | | |
| 12628 | | > volume flip #7 |
| 12629 | | |
| 12630 | | Opened cryosparc_P4_J244_003_volume_map_sharp.mrc z flip as #8, grid size |
| 12631 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 12632 | | |
| 12633 | | > close #7 |
| 12634 | | |
| 12635 | | > rename #8 CS_P4_J244_sharp_flip id #7 |
| 12636 | | |
| 12637 | | > save CS_P4_J244_sharp_flip.mrc #7 |
| 12638 | | |
| 12639 | | > color #7 #99BFE5 |
| 12640 | | |
| 12641 | | > volume #7 level 1.156 |
| 12642 | | |
| 12643 | | > save CS_P4_J244_sharp_flip.mrc #7 |
| 12644 | | |
| 12645 | | > save P4_J244_NU_sharp_flip.png supersample 3 transparentBackground true |
| 12646 | | |
| 12647 | | > save "/Users/peiyuyu/Documents/Goethe |
| 12648 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 12649 | | > F_B1/20260501_SA-F_B1_workflow.cxs" |
| 12650 | | |
| 12651 | | > open "/Users/peiyuyu/Documents/Goethe |
| 12652 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 12653 | | > F_B1/cryosparc_P4_J242_class_00_00122_volume_sharp.mrc" |
| 12654 | | |
| 12655 | | Opened cryosparc_P4_J242_class_00_00122_volume_sharp.mrc as #8, grid size |
| 12656 | | 128,128,128, pixel 2.08, shown at level 0.192, step 1, values float32 |
| 12657 | | |
| 12658 | | > hide #!7 models |
| 12659 | | |
| 12660 | | > volume #8 level 0.2208 |
| 12661 | | |
| 12662 | | > show #!7 models |
| 12663 | | |
| 12664 | | > select add #8 |
| 12665 | | |
| 12666 | | 2 models selected |
| 12667 | | |
| 12668 | | > view matrix models #8,1,0,0,1.7912,0,1,0,4.218,0,0,1,1.8536 |
| 12669 | | |
| 12670 | | > ui mousemode right "rotate selected models" |
| 12671 | | |
| 12672 | | > view matrix models |
| 12673 | | > #8,0.019063,-0.87425,-0.4851,301.31,0.055421,0.48537,-0.87255,194.56,0.99828,-0.010251,0.057705,27.748 |
| 12674 | | |
| 12675 | | > ui mousemode right "translate selected models" |
| 12676 | | |
| 12677 | | > view matrix models |
| 12678 | | > #8,0.019063,-0.87425,-0.4851,303.09,0.055421,0.48537,-0.87255,199.03,0.99828,-0.010251,0.057705,19.845 |
| 12679 | | |
| 12680 | | > view matrix models |
| 12681 | | > #8,0.019063,-0.87425,-0.4851,323.73,0.055421,0.48537,-0.87255,193.31,0.99828,-0.010251,0.057705,17.439 |
| 12682 | | |
| 12683 | | > view matrix models |
| 12684 | | > #8,0.019063,-0.87425,-0.4851,322.89,0.055421,0.48537,-0.87255,188.18,0.99828,-0.010251,0.057705,19.28 |
| 12685 | | |
| 12686 | | > fitmap #8 inMap #7 |
| 12687 | | |
| 12688 | | Fit map cryosparc_P4_J242_class_00_00122_volume_sharp.mrc in map |
| 12689 | | CS_P4_J244_sharp_flip.mrc using 14754 points |
| 12690 | | correlation = 0.481, correlation about mean = 0.07756, overlap = 1756 |
| 12691 | | steps = 112, shift = 13.3, angle = 15.3 degrees |
| 12692 | | |
| 12693 | | Position of cryosparc_P4_J242_class_00_00122_volume_sharp.mrc (#8) relative to |
| 12694 | | CS_P4_J244_sharp_flip.mrc (#7) coordinates: |
| 12695 | | Matrix rotation and translation |
| 12696 | | 0.05423844 -0.87969653 0.47243223 170.87663448 |
| 12697 | | 0.10002936 0.47553549 0.87399092 -59.90285646 |
| 12698 | | -0.99350507 -0.00014682 0.11378778 232.31099922 |
| 12699 | | Axis -0.44417982 0.74489379 0.49783284 |
| 12700 | | Axis point 199.16697689 -0.00000000 41.59458970 |
| 12701 | | Rotation angle (degrees) 100.26604714 |
| 12702 | | Shift along axis -4.86917491 |
| 12703 | | |
| 12704 | | |
| 12705 | | > select subtract #8 |
| 12706 | | |
| 12707 | | Nothing selected |
| 12708 | | |
| 12709 | | > hide #!7 models |
| 12710 | | |
| 12711 | | > open "/Users/peiyuyu/Documents/Goethe |
| 12712 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 12713 | | > F_B1/cryosparc_P4_J242_class_01_00122_volume_sharp.mrc" |
| 12714 | | |
| 12715 | | Opened cryosparc_P4_J242_class_01_00122_volume_sharp.mrc as #9, grid size |
| 12716 | | 128,128,128, pixel 2.08, shown at level 0.178, step 1, values float32 |
| 12717 | | |
| 12718 | | > hide #!8 models |
| 12719 | | |
| 12720 | | > volume #9 level 0.1464 |
| 12721 | | |
| 12722 | | > show #!8 models |
| 12723 | | |
| 12724 | | > select add #9 |
| 12725 | | |
| 12726 | | 2 models selected |
| 12727 | | |
| 12728 | | > ui mousemode right "rotate selected models" |
| 12729 | | |
| 12730 | | > view matrix models |
| 12731 | | > #9,0.01099,0.99675,0.079835,4.9565,0.1314,-0.080587,0.98805,-14.68,0.99127,-0.00036851,-0.13186,9.8 |
| 12732 | | |
| 12733 | | > ui mousemode right "translate selected models" |
| 12734 | | |
| 12735 | | > view matrix models |
| 12736 | | > #9,0.01099,0.99675,0.079835,10.903,0.1314,-0.080587,0.98805,5.6709,0.99127,-0.00036851,-0.13186,51.786 |
| 12737 | | |
| 12738 | | > view matrix models |
| 12739 | | > #9,0.01099,0.99675,0.079835,4.7224,0.1314,-0.080587,0.98805,-1.1935,0.99127,-0.00036851,-0.13186,45.788 |
| 12740 | | |
| 12741 | | > fitmap #9 inMap #8 |
| 12742 | | |
| 12743 | | Fit map cryosparc_P4_J242_class_01_00122_volume_sharp.mrc in map |
| 12744 | | cryosparc_P4_J242_class_00_00122_volume_sharp.mrc using 29766 points |
| 12745 | | correlation = 0.8496, correlation about mean = 0.3082, overlap = 1328 |
| 12746 | | steps = 284, shift = 18.3, angle = 53.9 degrees |
| 12747 | | |
| 12748 | | Position of cryosparc_P4_J242_class_01_00122_volume_sharp.mrc (#9) relative to |
| 12749 | | cryosparc_P4_J242_class_00_00122_volume_sharp.mrc (#8) coordinates: |
| 12750 | | Matrix rotation and translation |
| 12751 | | 0.99575722 -0.02668322 0.08806571 -8.52134797 |
| 12752 | | -0.09034177 -0.46538978 0.88048323 89.22150736 |
| 12753 | | 0.01749075 -0.88470354 -0.46582584 307.69453028 |
| 12754 | | Axis -0.99855360 0.03992375 -0.03601119 |
| 12755 | | Axis point 0.00000000 137.23273941 127.10917488 |
| 12756 | | Rotation angle (degrees) 117.88699495 |
| 12757 | | Shift along axis 0.99063260 |
| 12758 | | |
| 12759 | | |
| 12760 | | > select subtract #9 |
| 12761 | | |
| 12762 | | Nothing selected |
| 12763 | | |
| 12764 | | > hide #!8 models |
| 12765 | | |
| 12766 | | > hide #!9 models |
| 12767 | | |
| 12768 | | > open "/Users/peiyuyu/Documents/Goethe |
| 12769 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 12770 | | > F_B1/cryosparc_P4_J242_class_02_00122_volume_sharp.mrc" |
| 12771 | | |
| 12772 | | Opened cryosparc_P4_J242_class_02_00122_volume_sharp.mrc as #10, grid size |
| 12773 | | 128,128,128, pixel 2.08, shown at level 0.195, step 1, values float32 |
| 12774 | | |
| 12775 | | > select add #10 |
| 12776 | | |
| 12777 | | 2 models selected |
| 12778 | | |
| 12779 | | > show #!9 models |
| 12780 | | |
| 12781 | | > ui mousemode right "rotate selected models" |
| 12782 | | |
| 12783 | | > view matrix models |
| 12784 | | > #10,-0.17462,0.93232,0.31669,19.869,0.21917,0.35036,-0.91061,163.76,-0.95993,-0.089597,-0.26552,316.03 |
| 12785 | | |
| 12786 | | > ui mousemode right "translate selected models" |
| 12787 | | |
| 12788 | | > view matrix models |
| 12789 | | > #10,-0.17462,0.93232,0.31669,8.7975,0.21917,0.35036,-0.91061,193.69,-0.95993,-0.089597,-0.26552,324.3 |
| 12790 | | |
| 12791 | | > view matrix models |
| 12792 | | > #10,-0.17462,0.93232,0.31669,5.0809,0.21917,0.35036,-0.91061,186.44,-0.95993,-0.089597,-0.26552,324.52 |
| 12793 | | |
| 12794 | | > fitmap #10 inMap #9 |
| 12795 | | |
| 12796 | | Fit map cryosparc_P4_J242_class_02_00122_volume_sharp.mrc in map |
| 12797 | | cryosparc_P4_J242_class_01_00122_volume_sharp.mrc using 20970 points |
| 12798 | | correlation = 0.8182, correlation about mean = 0.3079, overlap = 1048 |
| 12799 | | steps = 212, shift = 18, angle = 29.3 degrees |
| 12800 | | |
| 12801 | | Position of cryosparc_P4_J242_class_02_00122_volume_sharp.mrc (#10) relative |
| 12802 | | to cryosparc_P4_J242_class_01_00122_volume_sharp.mrc (#9) coordinates: |
| 12803 | | Matrix rotation and translation |
| 12804 | | -0.92160797 -0.37293352 0.10751436 286.70013052 |
| 12805 | | -0.37621404 0.79028143 -0.48365097 141.61699812 |
| 12806 | | 0.09540306 -0.48618500 -0.86863249 299.79876520 |
| 12807 | | Axis -0.19795503 0.94611818 -0.25626978 |
| 12808 | | Axis point 158.67244900 0.00000000 168.59386356 |
| 12809 | | Rotation angle (degrees) 179.63327476 |
| 12810 | | Shift along axis 0.40332117 |
| 12811 | | |
| 12812 | | |
| 12813 | | > select subtract #10 |
| 12814 | | |
| 12815 | | Nothing selected |
| 12816 | | |
| 12817 | | > hide #!9 models |
| 12818 | | |
| 12819 | | > hide #!10 models |
| 12820 | | |
| 12821 | | > open "/Users/peiyuyu/Documents/Goethe |
| 12822 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 12823 | | > F_B1/cryosparc_P4_J242_class_03_00122_volume_sharp.mrc" |
| 12824 | | |
| 12825 | | Opened cryosparc_P4_J242_class_03_00122_volume_sharp.mrc as #11, grid size |
| 12826 | | 128,128,128, pixel 2.08, shown at level 0.218, step 1, values float32 |
| 12827 | | |
| 12828 | | > volume #11 level 0.1502 |
| 12829 | | |
| 12830 | | > show #!10 models |
| 12831 | | |
| 12832 | | > select add #11 |
| 12833 | | |
| 12834 | | 2 models selected |
| 12835 | | |
| 12836 | | > ui mousemode right "rotate selected models" |
| 12837 | | |
| 12838 | | > view matrix models |
| 12839 | | > #11,0.73904,-0.47164,0.48101,26.135,-0.11204,0.61802,0.77814,-47.481,-0.66427,-0.62897,0.4039,253.49 |
| 12840 | | |
| 12841 | | > ui mousemode right "translate selected models" |
| 12842 | | |
| 12843 | | > view matrix models |
| 12844 | | > #11,0.73904,-0.47164,0.48101,5.5926,-0.11204,0.61802,0.77814,-27.642,-0.66427,-0.62897,0.4039,266.06 |
| 12845 | | |
| 12846 | | > ui mousemode right "rotate selected models" |
| 12847 | | |
| 12848 | | > view matrix models |
| 12849 | | > #11,-0.56676,0.0073102,0.82385,78.335,0.19153,-0.97139,0.14038,216.5,0.80131,0.23736,0.54915,-63.542 |
| 12850 | | |
| 12851 | | > select subtract #11 |
| 12852 | | |
| 12853 | | Nothing selected |
| 12854 | | |
| 12855 | | > fitmap #11 inMap #10 |
| 12856 | | |
| 12857 | | Fit map cryosparc_P4_J242_class_03_00122_volume_sharp.mrc in map |
| 12858 | | cryosparc_P4_J242_class_02_00122_volume_sharp.mrc using 45754 points |
| 12859 | | correlation = 0.7333, correlation about mean = -0.04196, overlap = 1268 |
| 12860 | | steps = 132, shift = 23.8, angle = 15.3 degrees |
| 12861 | | |
| 12862 | | Position of cryosparc_P4_J242_class_03_00122_volume_sharp.mrc (#11) relative |
| 12863 | | to cryosparc_P4_J242_class_02_00122_volume_sharp.mrc (#10) coordinates: |
| 12864 | | Matrix rotation and translation |
| 12865 | | -0.26691235 0.13508665 -0.95420616 300.80664915 |
| 12866 | | -0.90776913 -0.36770197 0.20186745 274.07355373 |
| 12867 | | -0.32359389 0.92007981 0.22077169 18.93443182 |
| 12868 | | Axis 0.50771970 -0.44579332 -0.73721708 |
| 12869 | | Axis point 236.02557416 49.14154001 0.00000000 |
| 12870 | | Rotation angle (degrees) 134.98497404 |
| 12871 | | Shift along axis 16.58651491 |
| 12872 | | |
| 12873 | | |
| 12874 | | > hide #!10 models |
| 12875 | | |
| 12876 | | > open "/Users/peiyuyu/Documents/Goethe |
| 12877 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 12878 | | > F_B1/cryosparc_P4_J242_class_04_00122_volume_sharp.mrc" |
| 12879 | | |
| 12880 | | Opened cryosparc_P4_J242_class_04_00122_volume_sharp.mrc as #12, grid size |
| 12881 | | 128,128,128, pixel 2.08, shown at level 0.261, step 1, values float32 |
| 12882 | | |
| 12883 | | > show #!10 models |
| 12884 | | |
| 12885 | | > hide #!10 models |
| 12886 | | |
| 12887 | | > select add #12 |
| 12888 | | |
| 12889 | | 2 models selected |
| 12890 | | |
| 12891 | | > ui mousemode right "translate selected models" |
| 12892 | | |
| 12893 | | > view matrix models #12,1,0,0,-3.7479,0,1,0,3.9168,0,0,1,20.991 |
| 12894 | | |
| 12895 | | > ui mousemode right "rotate selected models" |
| 12896 | | |
| 12897 | | > view matrix models |
| 12898 | | > #12,0.79132,-0.13023,0.59737,-27.645,0.22144,0.97176,-0.081485,-12.263,-0.56989,0.19676,0.79781,93.603 |
| 12899 | | |
| 12900 | | > ui mousemode right "translate selected models" |
| 12901 | | |
| 12902 | | > view matrix models |
| 12903 | | > #12,0.79132,-0.13023,0.59737,-50.124,0.22144,0.97176,-0.081485,3.5285,-0.56989,0.19676,0.79781,102.68 |
| 12904 | | |
| 12905 | | > view matrix models |
| 12906 | | > #12,0.79132,-0.13023,0.59737,-48.335,0.22144,0.97176,-0.081485,2.277,-0.56989,0.19676,0.79781,101.98 |
| 12907 | | |
| 12908 | | > view matrix models |
| 12909 | | > #12,0.79132,-0.13023,0.59737,-41.566,0.22144,0.97176,-0.081485,-1.9637,-0.56989,0.19676,0.79781,101.41 |
| 12910 | | |
| 12911 | | > fitmap #12 inMap #11 |
| 12912 | | |
| 12913 | | Fit map cryosparc_P4_J242_class_04_00122_volume_sharp.mrc in map |
| 12914 | | cryosparc_P4_J242_class_03_00122_volume_sharp.mrc using 20956 points |
| 12915 | | correlation = 0.8514, correlation about mean = 0.04285, overlap = 1430 |
| 12916 | | steps = 80, shift = 7.16, angle = 11.7 degrees |
| 12917 | | |
| 12918 | | Position of cryosparc_P4_J242_class_04_00122_volume_sharp.mrc (#12) relative |
| 12919 | | to cryosparc_P4_J242_class_03_00122_volume_sharp.mrc (#11) coordinates: |
| 12920 | | Matrix rotation and translation |
| 12921 | | -0.93263188 0.26201641 0.24808299 197.07280134 |
| 12922 | | -0.29117819 -0.95255879 -0.08858337 308.05329962 |
| 12923 | | 0.21310334 -0.15485204 0.96468016 17.95641726 |
| 12924 | | Axis -0.11870856 0.06265985 -0.99095006 |
| 12925 | | Axis point 119.69175871 140.62215054 0.00000000 |
| 12926 | | Rotation angle (degrees) 163.79210928 |
| 12927 | | Shift along axis -21.88556717 |
| 12928 | | |
| 12929 | | |
| 12930 | | > select subtract #12 |
| 12931 | | |
| 12932 | | Nothing selected |
| 12933 | | |
| 12934 | | > hide #!11 models |
| 12935 | | |
| 12936 | | > volume #12 level 0.4033 |
| 12937 | | |
| 12938 | | > volume #12 level 0.3453 |
| 12939 | | |
| 12940 | | > color #8 #FCEDFF |
| 12941 | | |
| 12942 | | > hide #!12 models |
| 12943 | | |
| 12944 | | > show #!8 models |
| 12945 | | |
| 12946 | | > hide #!8 models |
| 12947 | | |
| 12948 | | > show #!9 models |
| 12949 | | |
| 12950 | | > color #9 #FCEDFF |
| 12951 | | |
| 12952 | | > hide #!9 models |
| 12953 | | |
| 12954 | | > show #!10 models |
| 12955 | | |
| 12956 | | > color #10 #FCEDFF |
| 12957 | | |
| 12958 | | > hide #!10 models |
| 12959 | | |
| 12960 | | > show #!11 models |
| 12961 | | |
| 12962 | | > color #11 #FCEDFF |
| 12963 | | |
| 12964 | | > hide #!11 models |
| 12965 | | |
| 12966 | | > show #!12 models |
| 12967 | | |
| 12968 | | > color #12 #99BFE5 |
| 12969 | | |
| 12970 | | > view P1 |
| 12971 | | |
| 12972 | | > show #!2 models |
| 12973 | | |
| 12974 | | > hide #!2 models |
| 12975 | | |
| 12976 | | > show #!3 models |
| 12977 | | |
| 12978 | | > hide #!3 models |
| 12979 | | |
| 12980 | | > show #!4 models |
| 12981 | | |
| 12982 | | > hide #!4 models |
| 12983 | | |
| 12984 | | > show #!1 models |
| 12985 | | |
| 12986 | | > hide #!1 models |
| 12987 | | |
| 12988 | | > show #!6 models |
| 12989 | | |
| 12990 | | > hide #!6 models |
| 12991 | | |
| 12992 | | > show #!3 models |
| 12993 | | |
| 12994 | | > select add #12 |
| 12995 | | |
| 12996 | | 2 models selected |
| 12997 | | |
| 12998 | | > ui mousemode right "rotate selected models" |
| 12999 | | |
| 13000 | | > view matrix models |
| 13001 | | > #12,0.95369,-0.29732,-0.045496,35.025,0.29889,0.95373,0.032695,-17.906,0.03367,-0.044779,0.99843,26.075 |
| 13002 | | |
| 13003 | | > view matrix models |
| 13004 | | > #12,-0.8879,0.32078,-0.32974,233.6,0.30063,0.94715,0.1119,-26.299,0.34821,0.00022498,-0.93742,198.42 |
| 13005 | | |
| 13006 | | > ui mousemode right "translate selected models" |
| 13007 | | |
| 13008 | | > view matrix models |
| 13009 | | > #12,-0.8879,0.32078,-0.32974,232.89,0.30063,0.94715,0.1119,-34.317,0.34821,0.00022498,-0.93742,213.72 |
| 13010 | | |
| 13011 | | > view matrix models |
| 13012 | | > #12,-0.8879,0.32078,-0.32974,243.25,0.30063,0.94715,0.1119,-55.273,0.34821,0.00022498,-0.93742,214.76 |
| 13013 | | |
| 13014 | | > ui mousemode right "rotate selected models" |
| 13015 | | |
| 13016 | | > view matrix models |
| 13017 | | > #12,-0.88731,0.39919,0.23093,169.34,0.40717,0.91324,-0.014156,-50.796,-0.21654,0.081466,-0.97287,283.71 |
| 13018 | | |
| 13019 | | > fitmap #12 inMap #3 |
| 13020 | | |
| 13021 | | Fit map cryosparc_P4_J242_class_04_00122_volume_sharp.mrc in map |
| 13022 | | CS_P4_J216_class_2_sharp_flip.mrc using 12546 points |
| 13023 | | correlation = 0.9893, correlation about mean = 0.7632, overlap = 2433 |
| 13024 | | steps = 124, shift = 11, angle = 27.9 degrees |
| 13025 | | |
| 13026 | | Position of cryosparc_P4_J242_class_04_00122_volume_sharp.mrc (#12) relative |
| 13027 | | to CS_P4_J216_class_2_sharp_flip.mrc (#3) coordinates: |
| 13028 | | Matrix rotation and translation |
| 13029 | | -0.62359754 0.77535022 0.09979057 101.14085164 |
| 13030 | | 0.77719680 0.62864768 -0.02769904 -50.42984630 |
| 13031 | | -0.08420957 0.06028386 -0.99462285 268.88184712 |
| 13032 | | Axis 0.43136931 0.90213001 0.00905357 |
| 13033 | | Axis point 70.24601107 0.00000000 131.83510298 |
| 13034 | | Rotation angle (degrees) 174.14674629 |
| 13035 | | Shift along axis 0.56912319 |
| 13036 | | |
| 13037 | | |
| 13038 | | > select subtract #12 |
| 13039 | | |
| 13040 | | Nothing selected |
| 13041 | | |
| 13042 | | > hide #!12 models |
| 13043 | | |
| 13044 | | > show #!12 models |
| 13045 | | |
| 13046 | | > show #!11 models |
| 13047 | | |
| 13048 | | > select add #11 |
| 13049 | | |
| 13050 | | 2 models selected |
| 13051 | | |
| 13052 | | > view matrix models |
| 13053 | | > #11,-0.37852,0.89247,0.24538,28.817,0.88041,0.42898,-0.20213,16.101,-0.28566,0.13952,-0.94812,300.03 |
| 13054 | | |
| 13055 | | > ui mousemode right "translate selected models" |
| 13056 | | |
| 13057 | | > view matrix models |
| 13058 | | > #11,-0.37852,0.89247,0.24538,46.8,0.88041,0.42898,-0.20213,-5.6117,-0.28566,0.13952,-0.94812,300.85 |
| 13059 | | |
| 13060 | | > view matrix models |
| 13061 | | > #11,-0.37852,0.89247,0.24538,36.63,0.88041,0.42898,-0.20213,-16.953,-0.28566,0.13952,-0.94812,296.6 |
| 13062 | | |
| 13063 | | > fitmap #11 inMap #12 |
| 13064 | | |
| 13065 | | Fit map cryosparc_P4_J242_class_03_00122_volume_sharp.mrc in map |
| 13066 | | cryosparc_P4_J242_class_04_00122_volume_sharp.mrc using 45754 points |
| 13067 | | correlation = 0.7192, correlation about mean = 0.1299, overlap = 1714 |
| 13068 | | steps = 96, shift = 6.98, angle = 12.7 degrees |
| 13069 | | |
| 13070 | | Position of cryosparc_P4_J242_class_03_00122_volume_sharp.mrc (#11) relative |
| 13071 | | to cryosparc_P4_J242_class_04_00122_volume_sharp.mrc (#12) coordinates: |
| 13072 | | Matrix rotation and translation |
| 13073 | | 0.88557701 -0.25567312 -0.38779454 99.27082409 |
| 13074 | | 0.32552043 0.93717068 0.12548928 -53.44589150 |
| 13075 | | 0.33134544 -0.23736546 0.91316364 -22.95393091 |
| 13076 | | Axis -0.36530839 -0.72400286 0.58512361 |
| 13077 | | Axis point 106.02252826 0.00000000 214.34257569 |
| 13078 | | Rotation angle (degrees) 29.77806268 |
| 13079 | | Shift along axis -11.00037360 |
| 13080 | | |
| 13081 | | |
| 13082 | | > select subtract #11 |
| 13083 | | |
| 13084 | | Nothing selected |
| 13085 | | |
| 13086 | | > hide #!12 models |
| 13087 | | |
| 13088 | | > show #!12 models |
| 13089 | | |
| 13090 | | > hide #!12 models |
| 13091 | | |
| 13092 | | > hide #!3 models |
| 13093 | | |
| 13094 | | > show #!12 models |
| 13095 | | |
| 13096 | | > hide #!11 models |
| 13097 | | |
| 13098 | | > show #!10 models |
| 13099 | | |
| 13100 | | > select add #10 |
| 13101 | | |
| 13102 | | 2 models selected |
| 13103 | | |
| 13104 | | > view matrix models |
| 13105 | | > #10,-0.49822,0.81176,0.30466,37.524,-0.14214,0.27015,-0.95227,247.33,-0.85532,-0.51775,-0.019207,343.36 |
| 13106 | | |
| 13107 | | > ui mousemode right "rotate selected models" |
| 13108 | | |
| 13109 | | > view matrix models |
| 13110 | | > #10,-0.013344,0.85199,0.52339,-68.961,-0.1627,-0.51831,0.83957,128.11,0.98659,-0.073951,0.14553,-15.381 |
| 13111 | | |
| 13112 | | > ui mousemode right "translate selected models" |
| 13113 | | |
| 13114 | | > view matrix models |
| 13115 | | > #10,-0.013344,0.85199,0.52339,-45.161,-0.1627,-0.51831,0.83957,119.13,0.98659,-0.073951,0.14553,-13.578 |
| 13116 | | |
| 13117 | | > fitmap #10 inMap #12 |
| 13118 | | |
| 13119 | | Fit map cryosparc_P4_J242_class_02_00122_volume_sharp.mrc in map |
| 13120 | | cryosparc_P4_J242_class_04_00122_volume_sharp.mrc using 20970 points |
| 13121 | | correlation = 0.7101, correlation about mean = 0.02358, overlap = 908.6 |
| 13122 | | steps = 80, shift = 7.98, angle = 9.33 degrees |
| 13123 | | |
| 13124 | | Position of cryosparc_P4_J242_class_02_00122_volume_sharp.mrc (#10) relative |
| 13125 | | to cryosparc_P4_J242_class_04_00122_volume_sharp.mrc (#12) coordinates: |
| 13126 | | Matrix rotation and translation |
| 13127 | | -0.35385865 -0.88893073 0.29083707 267.80089120 |
| 13128 | | -0.02210060 0.31881642 0.94755878 -31.84809054 |
| 13129 | | -0.93503776 0.32887420 -0.13246184 232.75133151 |
| 13130 | | Axis -0.38099533 0.75491227 0.53380711 |
| 13131 | | Axis point 205.22642342 0.00000000 36.94010088 |
| 13132 | | Rotation angle (degrees) 125.71487620 |
| 13133 | | Shift along axis -1.82908632 |
| 13134 | | |
| 13135 | | |
| 13136 | | > ui mousemode right "rotate selected models" |
| 13137 | | |
| 13138 | | > view matrix models |
| 13139 | | > #10,-0.12287,-0.47662,0.87048,95.543,0.19038,-0.87215,-0.45067,269.26,0.97399,0.11035,0.1979,-37.789 |
| 13140 | | |
| 13141 | | > select subtract #10 |
| 13142 | | |
| 13143 | | Nothing selected |
| 13144 | | |
| 13145 | | > hide #!12 models |
| 13146 | | |
| 13147 | | > show #!11 models |
| 13148 | | |
| 13149 | | > show #!12 models |
| 13150 | | |
| 13151 | | > hide #!10 models |
| 13152 | | |
| 13153 | | > hide #!12 models |
| 13154 | | |
| 13155 | | > show #!10 models |
| 13156 | | |
| 13157 | | > select add #10 |
| 13158 | | |
| 13159 | | 2 models selected |
| 13160 | | |
| 13161 | | > view matrix models |
| 13162 | | > #10,-0.38685,-0.21971,0.89558,99.669,-0.053858,-0.96416,-0.2598,294.54,0.92057,-0.14874,0.36115,-16.709 |
| 13163 | | |
| 13164 | | > fitmap #10 inMap #11 |
| 13165 | | |
| 13166 | | Fit map cryosparc_P4_J242_class_02_00122_volume_sharp.mrc in map |
| 13167 | | cryosparc_P4_J242_class_03_00122_volume_sharp.mrc using 20970 points |
| 13168 | | correlation = 0.8482, correlation about mean = 0.1221, overlap = 870.1 |
| 13169 | | steps = 88, shift = 3.31, angle = 12.5 degrees |
| 13170 | | |
| 13171 | | Position of cryosparc_P4_J242_class_02_00122_volume_sharp.mrc (#10) relative |
| 13172 | | to cryosparc_P4_J242_class_03_00122_volume_sharp.mrc (#11) coordinates: |
| 13173 | | Matrix rotation and translation |
| 13174 | | -0.32110732 -0.67353914 -0.66575904 357.49560201 |
| 13175 | | 0.09667773 -0.72262784 0.68444314 122.97291760 |
| 13176 | | -0.94209527 0.15541563 0.29715733 219.46626263 |
| 13177 | | Axis -0.54292343 0.28359472 0.79044809 |
| 13178 | | Axis point 234.55310205 82.20310947 0.00000000 |
| 13179 | | Rotation angle (degrees) 150.84311237 |
| 13180 | | Shift along axis 14.25842210 |
| 13181 | | |
| 13182 | | |
| 13183 | | > view matrix models |
| 13184 | | > #10,-0.30509,0.38504,-0.87101,233.77,0.18733,0.92101,0.34153,-54.422,0.93372,-0.058973,-0.35312,61.617 |
| 13185 | | |
| 13186 | | > ui mousemode right "translate selected models" |
| 13187 | | |
| 13188 | | > view matrix models |
| 13189 | | > #10,-0.30509,0.38504,-0.87101,240.35,0.18733,0.92101,0.34153,-58.712,0.93372,-0.058973,-0.35312,49.559 |
| 13190 | | |
| 13191 | | > view matrix models |
| 13192 | | > #10,-0.30509,0.38504,-0.87101,238.16,0.18733,0.92101,0.34153,-58.03,0.93372,-0.058973,-0.35312,51.895 |
| 13193 | | |
| 13194 | | > ui mousemode right "rotate selected models" |
| 13195 | | |
| 13196 | | > view matrix models |
| 13197 | | > #10,-0.36524,0.52001,-0.77213,217.65,0.08187,0.84416,0.5298,-55.56,0.9273,0.13029,-0.3509,28.264 |
| 13198 | | |
| 13199 | | > fitmap #10 inMap #11 |
| 13200 | | |
| 13201 | | Fit map cryosparc_P4_J242_class_02_00122_volume_sharp.mrc in map |
| 13202 | | cryosparc_P4_J242_class_03_00122_volume_sharp.mrc using 20970 points |
| 13203 | | correlation = 0.8485, correlation about mean = 0.1929, overlap = 854.7 |
| 13204 | | steps = 140, shift = 18.2, angle = 22.5 degrees |
| 13205 | | |
| 13206 | | Position of cryosparc_P4_J242_class_02_00122_volume_sharp.mrc (#10) relative |
| 13207 | | to cryosparc_P4_J242_class_03_00122_volume_sharp.mrc (#11) coordinates: |
| 13208 | | Matrix rotation and translation |
| 13209 | | -0.33967352 0.22689098 0.91276633 25.87088295 |
| 13210 | | 0.00914596 0.97121791 -0.23801704 28.63290177 |
| 13211 | | -0.94049893 -0.07249996 -0.33197214 335.97765353 |
| 13212 | | Axis 0.08835402 0.98928424 -0.11623361 |
| 13213 | | Axis point 129.11230783 0.00000000 160.33867701 |
| 13214 | | Rotation angle (degrees) 110.50039702 |
| 13215 | | Shift along axis -8.44002041 |
| 13216 | | |
| 13217 | | |
| 13218 | | > view matrix models |
| 13219 | | > #10,-0.23006,0.48061,-0.84622,212.13,0.22993,0.87177,0.43261,-89.291,0.94562,-0.095042,-0.31107,58.162 |
| 13220 | | |
| 13221 | | > ui mousemode right "translate selected models" |
| 13222 | | |
| 13223 | | > view matrix models |
| 13224 | | > #10,-0.23006,0.48061,-0.84622,206.51,0.22993,0.87177,0.43261,-81.849,0.94562,-0.095042,-0.31107,43.385 |
| 13225 | | |
| 13226 | | > view matrix models |
| 13227 | | > #10,-0.23006,0.48061,-0.84622,209.88,0.22993,0.87177,0.43261,-83.853,0.94562,-0.095042,-0.31107,44.292 |
| 13228 | | |
| 13229 | | > select subtract #10 |
| 13230 | | |
| 13231 | | Nothing selected |
| 13232 | | |
| 13233 | | > hide #!10 models |
| 13234 | | |
| 13235 | | > hide #!11 models |
| 13236 | | |
| 13237 | | > show #!10 models |
| 13238 | | |
| 13239 | | > hide #!10 models |
| 13240 | | |
| 13241 | | > show #!9 models |
| 13242 | | |
| 13243 | | > show #!10 models |
| 13244 | | |
| 13245 | | > select add #9 |
| 13246 | | |
| 13247 | | 2 models selected |
| 13248 | | |
| 13249 | | > view matrix models |
| 13250 | | > #9,0.18919,0.68161,-0.70683,127.56,-0.072128,0.72753,0.68227,-34.663,0.97929,-0.078095,0.1868,-5.196 |
| 13251 | | |
| 13252 | | > ui mousemode right "rotate selected models" |
| 13253 | | |
| 13254 | | > view matrix models |
| 13255 | | > #9,0.094504,0.99539,-0.016524,13.65,-0.46578,0.02954,-0.88441,305.71,-0.87984,0.091276,0.46642,193.56 |
| 13256 | | |
| 13257 | | > ui mousemode right "translate selected models" |
| 13258 | | |
| 13259 | | > view matrix models |
| 13260 | | > #9,0.094504,0.99539,-0.016524,15.801,-0.46578,0.02954,-0.88441,306.87,-0.87984,0.091276,0.46642,176.78 |
| 13261 | | |
| 13262 | | > view matrix models |
| 13263 | | > #9,0.094504,0.99539,-0.016524,12.042,-0.46578,0.02954,-0.88441,307.73,-0.87984,0.091276,0.46642,167.03 |
| 13264 | | |
| 13265 | | > ui mousemode right "rotate selected models" |
| 13266 | | |
| 13267 | | > view matrix models |
| 13268 | | > #9,0.21351,-0.97318,0.085687,220.62,-0.11603,0.061829,0.99132,14.069,-0.97003,-0.2216,-0.099713,290.21 |
| 13269 | | |
| 13270 | | > ui mousemode right "translate selected models" |
| 13271 | | |
| 13272 | | > view matrix models |
| 13273 | | > #9,0.21351,-0.97318,0.085687,223.99,-0.11603,0.061829,0.99132,9.6501,-0.97003,-0.2216,-0.099713,279.71 |
| 13274 | | |
| 13275 | | > fitmap #9 inMap #10 |
| 13276 | | |
| 13277 | | Fit map cryosparc_P4_J242_class_01_00122_volume_sharp.mrc in map |
| 13278 | | cryosparc_P4_J242_class_02_00122_volume_sharp.mrc using 29766 points |
| 13279 | | correlation = 0.8261, correlation about mean = 0.3518, overlap = 1268 |
| 13280 | | steps = 228, shift = 10.5, angle = 43.8 degrees |
| 13281 | | |
| 13282 | | Position of cryosparc_P4_J242_class_01_00122_volume_sharp.mrc (#9) relative to |
| 13283 | | cryosparc_P4_J242_class_02_00122_volume_sharp.mrc (#10) coordinates: |
| 13284 | | Matrix rotation and translation |
| 13285 | | -0.79231406 -0.50183310 0.34698987 259.09489231 |
| 13286 | | 0.60835065 -0.60661249 0.51179172 70.83831660 |
| 13287 | | -0.04634564 0.61659129 0.78591810 -47.09681091 |
| 13288 | | Axis 0.08862868 0.33264264 0.93887903 |
| 13289 | | Axis point 119.55771099 88.88471782 0.00000000 |
| 13290 | | Rotation angle (degrees) 143.75577737 |
| 13291 | | Shift along axis 2.30887434 |
| 13292 | | |
| 13293 | | |
| 13294 | | > select subtract #9 |
| 13295 | | |
| 13296 | | Nothing selected |
| 13297 | | |
| 13298 | | > hide #!9 models |
| 13299 | | |
| 13300 | | > hide #!10 models |
| 13301 | | |
| 13302 | | > show #!9 models |
| 13303 | | |
| 13304 | | > show #!10 models |
| 13305 | | |
| 13306 | | > hide #!10 models |
| 13307 | | |
| 13308 | | > show #!8 models |
| 13309 | | |
| 13310 | | > hide #!9 models |
| 13311 | | |
| 13312 | | > show #!9 models |
| 13313 | | |
| 13314 | | > select add #8 |
| 13315 | | |
| 13316 | | 2 models selected |
| 13317 | | |
| 13318 | | > ui mousemode right "rotate selected models" |
| 13319 | | |
| 13320 | | > view matrix models |
| 13321 | | > #8,0.57009,0.77789,0.26434,-76.801,0.087184,0.26265,-0.96094,232.01,-0.81694,0.57087,0.081917,150.81 |
| 13322 | | |
| 13323 | | > ui mousemode right "translate selected models" |
| 13324 | | |
| 13325 | | > view matrix models |
| 13326 | | > #8,0.57009,0.77789,0.26434,-65.422,0.087184,0.26265,-0.96094,221.32,-0.81694,0.57087,0.081917,140.75 |
| 13327 | | |
| 13328 | | > view matrix models |
| 13329 | | > #8,0.57009,0.77789,0.26434,-76.259,0.087184,0.26265,-0.96094,220.33,-0.81694,0.57087,0.081917,139.28 |
| 13330 | | |
| 13331 | | > ui mousemode right "rotate selected models" |
| 13332 | | |
| 13333 | | > view matrix models |
| 13334 | | > #8,0.35163,-0.43011,0.83148,20.753,0.096433,0.90012,0.42483,-70.009,-0.93116,-0.069204,0.35799,194.15 |
| 13335 | | |
| 13336 | | > ui mousemode right "translate selected models" |
| 13337 | | |
| 13338 | | > view matrix models |
| 13339 | | > #8,0.35163,-0.43011,0.83148,32.249,0.096433,0.90012,0.42483,-69.075,-0.93116,-0.069204,0.35799,199.19 |
| 13340 | | |
| 13341 | | > fitmap #8 inMap #9 |
| 13342 | | |
| 13343 | | Fit map cryosparc_P4_J242_class_00_00122_volume_sharp.mrc in map |
| 13344 | | cryosparc_P4_J242_class_01_00122_volume_sharp.mrc using 14754 points |
| 13345 | | correlation = 0.886, correlation about mean = 0.3322, overlap = 930.7 |
| 13346 | | steps = 164, shift = 11.7, angle = 31.5 degrees |
| 13347 | | |
| 13348 | | Position of cryosparc_P4_J242_class_00_00122_volume_sharp.mrc (#8) relative to |
| 13349 | | cryosparc_P4_J242_class_01_00122_volume_sharp.mrc (#9) coordinates: |
| 13350 | | Matrix rotation and translation |
| 13351 | | 0.99392076 -0.10814474 0.02064566 12.78899705 |
| 13352 | | -0.02517844 -0.40582068 -0.91360584 308.93876287 |
| 13353 | | 0.10718010 0.90753198 -0.40607651 52.31471926 |
| 13354 | | Axis 0.99784036 -0.04741407 0.04545901 |
| 13355 | | Axis point 0.00000000 137.80284162 126.13315110 |
| 13356 | | Rotation angle (degrees) 114.14129203 |
| 13357 | | Shift along axis 0.49150880 |
| 13358 | | |
| 13359 | | |
| 13360 | | > select subtract #8 |
| 13361 | | |
| 13362 | | Nothing selected |
| 13363 | | |
| 13364 | | > hide #!9 models |
| 13365 | | |
| 13366 | | > show #!9 models |
| 13367 | | |
| 13368 | | > hide #!8 models |
| 13369 | | |
| 13370 | | > show #!8 models |
| 13371 | | |
| 13372 | | > hide #!9 models |
| 13373 | | |
| 13374 | | > view P1 |
| 13375 | | |
| 13376 | | > show #!9 models |
| 13377 | | |
| 13378 | | > hide #!8 models |
| 13379 | | |
| 13380 | | > hide #!9 models |
| 13381 | | |
| 13382 | | > show #!10 models |
| 13383 | | |
| 13384 | | > hide #!10 models |
| 13385 | | |
| 13386 | | > show #!11 models |
| 13387 | | |
| 13388 | | > hide #!11 models |
| 13389 | | |
| 13390 | | > show #!12 models |
| 13391 | | |
| 13392 | | > hide #!12 models |
| 13393 | | |
| 13394 | | > show #!8 models |
| 13395 | | |
| 13396 | | > show #!12 models |
| 13397 | | |
| 13398 | | > view P1 |
| 13399 | | |
| 13400 | | > select add #8 |
| 13401 | | |
| 13402 | | 2 models selected |
| 13403 | | |
| 13404 | | > ui mousemode right "rotate selected models" |
| 13405 | | |
| 13406 | | > view matrix models |
| 13407 | | > #8,0.17056,0.37082,0.91291,-61.936,0.033776,0.92374,-0.38153,50.195,-0.98477,0.095906,0.14502,217.51 |
| 13408 | | |
| 13409 | | > select subtract #8 |
| 13410 | | |
| 13411 | | Nothing selected |
| 13412 | | |
| 13413 | | > hide #!12 models |
| 13414 | | |
| 13415 | | > show #!9 models |
| 13416 | | |
| 13417 | | > select add #9 |
| 13418 | | |
| 13419 | | 2 models selected |
| 13420 | | |
| 13421 | | > view matrix models |
| 13422 | | > #9,0.22431,-0.86548,-0.44791,277.63,0.10958,-0.43431,0.89407,50.356,-0.96834,-0.24963,-0.0025846,282.76 |
| 13423 | | |
| 13424 | | > fitmap #9 inMap #8 |
| 13425 | | |
| 13426 | | Fit map cryosparc_P4_J242_class_01_00122_volume_sharp.mrc in map |
| 13427 | | cryosparc_P4_J242_class_00_00122_volume_sharp.mrc using 29766 points |
| 13428 | | correlation = 0.8496, correlation about mean = 0.3079, overlap = 1328 |
| 13429 | | steps = 196, shift = 4.92, angle = 23.1 degrees |
| 13430 | | |
| 13431 | | Position of cryosparc_P4_J242_class_01_00122_volume_sharp.mrc (#9) relative to |
| 13432 | | cryosparc_P4_J242_class_00_00122_volume_sharp.mrc (#8) coordinates: |
| 13433 | | Matrix rotation and translation |
| 13434 | | 0.99577299 -0.02696715 0.08780046 -8.42294333 |
| 13435 | | -0.09023986 -0.46536786 0.88050527 89.20116331 |
| 13436 | | 0.01711480 -0.88470646 -0.46583426 307.74421532 |
| 13437 | | Axis -0.99855897 0.03998603 -0.03579261 |
| 13438 | | Axis point 0.00000000 137.24012810 127.13677639 |
| 13439 | | Rotation angle (degrees) 117.88604570 |
| 13440 | | Shift along axis 0.96263749 |
| 13441 | | |
| 13442 | | |
| 13443 | | > select subtract #9 |
| 13444 | | |
| 13445 | | Nothing selected |
| 13446 | | |
| 13447 | | > select add #9 |
| 13448 | | |
| 13449 | | 2 models selected |
| 13450 | | |
| 13451 | | > select subtract #9 |
| 13452 | | |
| 13453 | | Nothing selected |
| 13454 | | |
| 13455 | | > hide #!8 models |
| 13456 | | |
| 13457 | | > hide #!9 models |
| 13458 | | |
| 13459 | | > show #!9 models |
| 13460 | | |
| 13461 | | > show #!10 models |
| 13462 | | |
| 13463 | | > show #!8 models |
| 13464 | | |
| 13465 | | > hide #!9 models |
| 13466 | | |
| 13467 | | > hide #!8 models |
| 13468 | | |
| 13469 | | > show #!9 models |
| 13470 | | |
| 13471 | | > select add #10 |
| 13472 | | |
| 13473 | | 2 models selected |
| 13474 | | |
| 13475 | | > view matrix models |
| 13476 | | > #10,-0.15057,0.45945,-0.87535,204.11,-0.21614,0.84872,0.48265,-19.059,0.96468,0.26187,-0.028487,-39.785 |
| 13477 | | |
| 13478 | | > fitmap #10 inMap #8 |
| 13479 | | |
| 13480 | | Fit map cryosparc_P4_J242_class_02_00122_volume_sharp.mrc in map |
| 13481 | | cryosparc_P4_J242_class_00_00122_volume_sharp.mrc using 20970 points |
| 13482 | | correlation = 0.8656, correlation about mean = 0.3963, overlap = 1232 |
| 13483 | | steps = 148, shift = 6.34, angle = 30.8 degrees |
| 13484 | | |
| 13485 | | Position of cryosparc_P4_J242_class_02_00122_volume_sharp.mrc (#10) relative |
| 13486 | | to cryosparc_P4_J242_class_00_00122_volume_sharp.mrc (#8) coordinates: |
| 13487 | | Matrix rotation and translation |
| 13488 | | -0.99459983 0.06769619 -0.07866640 263.08124995 |
| 13489 | | 0.09074118 0.93510189 -0.34256458 42.75279763 |
| 13490 | | 0.05037079 -0.34785296 -0.93619501 298.73878944 |
| 13491 | | Axis -0.04031220 -0.98362390 0.17566718 |
| 13492 | | Axis point 125.67832095 0.00000000 157.57753081 |
| 13493 | | Rotation angle (degrees) 176.23911220 |
| 13494 | | Shift along axis -0.17945663 |
| 13495 | | |
| 13496 | | |
| 13497 | | > show #!8 models |
| 13498 | | |
| 13499 | | > hide #!9 models |
| 13500 | | |
| 13501 | | > select subtract #10 |
| 13502 | | |
| 13503 | | Nothing selected |
| 13504 | | |
| 13505 | | > hide #!10 models |
| 13506 | | |
| 13507 | | > show #!10 models |
| 13508 | | |
| 13509 | | > hide #!8 models |
| 13510 | | |
| 13511 | | > show #!8 models |
| 13512 | | |
| 13513 | | > hide #!8 models |
| 13514 | | |
| 13515 | | > show #!11 models |
| 13516 | | |
| 13517 | | > hide #!10 models |
| 13518 | | |
| 13519 | | > show #!10 models |
| 13520 | | |
| 13521 | | > select add #11 |
| 13522 | | |
| 13523 | | 2 models selected |
| 13524 | | |
| 13525 | | > view matrix models |
| 13526 | | > #11,0.83789,-0.53148,-0.12435,93.216,-0.53452,-0.84509,0.010318,306.1,-0.11057,0.05782,-0.99219,291.46 |
| 13527 | | |
| 13528 | | > view matrix models |
| 13529 | | > #11,0.87567,0.38049,0.29737,-84.014,0.47018,-0.53126,-0.70477,229.85,-0.11018,0.75696,-0.64411,156.03 |
| 13530 | | |
| 13531 | | > view matrix models |
| 13532 | | > #11,0.99273,0.070823,-0.097356,-6.1982,0.016369,-0.88055,-0.47367,302.86,-0.11927,0.46863,-0.87531,225.51 |
| 13533 | | |
| 13534 | | > fitmap #11 inMap #10 |
| 13535 | | |
| 13536 | | Fit map cryosparc_P4_J242_class_03_00122_volume_sharp.mrc in map |
| 13537 | | cryosparc_P4_J242_class_02_00122_volume_sharp.mrc using 45754 points |
| 13538 | | correlation = 0.7386, correlation about mean = -0.02581, overlap = 1355 |
| 13539 | | steps = 168, shift = 17.3, angle = 31.8 degrees |
| 13540 | | |
| 13541 | | Position of cryosparc_P4_J242_class_03_00122_volume_sharp.mrc (#11) relative |
| 13542 | | to cryosparc_P4_J242_class_02_00122_volume_sharp.mrc (#10) coordinates: |
| 13543 | | Matrix rotation and translation |
| 13544 | | -0.38917965 0.05095816 -0.91975131 323.90582539 |
| 13545 | | -0.15837916 -0.98730156 0.01231516 285.52055130 |
| 13546 | | -0.90744434 0.15046225 0.39230839 175.68159882 |
| 13547 | | Axis 0.55013716 -0.04900950 -0.83363492 |
| 13548 | | Axis point 230.74773970 125.72052254 0.00000000 |
| 13549 | | Rotation angle (degrees) 172.78707980 |
| 13550 | | Shift along axis 17.74509610 |
| 13551 | | |
| 13552 | | |
| 13553 | | > select subtract #11 |
| 13554 | | |
| 13555 | | Nothing selected |
| 13556 | | |
| 13557 | | > hide #!11 models |
| 13558 | | |
| 13559 | | > hide #!10 models |
| 13560 | | |
| 13561 | | > show #!11 models |
| 13562 | | |
| 13563 | | > hide #!11 models |
| 13564 | | |
| 13565 | | > show #!10 models |
| 13566 | | |
| 13567 | | > hide #!10 models |
| 13568 | | |
| 13569 | | > show #!9 models |
| 13570 | | |
| 13571 | | > hide #!9 models |
| 13572 | | |
| 13573 | | > view P1 |
| 13574 | | |
| 13575 | | > show #!12 models |
| 13576 | | |
| 13577 | | > ui mousemode right translate |
| 13578 | | |
| 13579 | | > save P4_J242_class_4_sharp.png supersample 3 transparentBackground true |
| 13580 | | |
| 13581 | | > hide #!12 models |
| 13582 | | |
| 13583 | | > show #!11 models |
| 13584 | | |
| 13585 | | > save P4_J242_class_3_sharp.png supersample 3 transparentBackground true |
| 13586 | | |
| 13587 | | > hide #!11 models |
| 13588 | | |
| 13589 | | > show #!10 models |
| 13590 | | |
| 13591 | | > save P4_J242_class_2_sharp.png supersample 3 transparentBackground true |
| 13592 | | |
| 13593 | | > hide #!10 models |
| 13594 | | |
| 13595 | | > show #!9 models |
| 13596 | | |
| 13597 | | > view P1 |
| 13598 | | |
| 13599 | | [Repeated 1 time(s)] |
| 13600 | | |
| 13601 | | > save P4_J242_class_1_sharp.png supersample 3 transparentBackground true |
| 13602 | | |
| 13603 | | > hide #!9 models |
| 13604 | | |
| 13605 | | > show #!8 models |
| 13606 | | |
| 13607 | | > save P4_J242_class_0_sharp.png supersample 3 transparentBackground true |
| 13608 | | |
| 13609 | | > save "/Users/peiyuyu/Documents/Goethe |
| 13610 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 13611 | | > F_B1/20260501_SA-F_B1_workflow.cxs" |
| 13612 | | |
| 13613 | | Unsupported scale factor (0.000000) detected on Display1 |
| 13614 | | |
| 13615 | | [Repeated 2 time(s)] |
| 13616 | | |
| 13617 | | > open "/Users/peiyuyu/Documents/Goethe |
| 13618 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 13619 | | > F_B1/cryosparc_P4_J117_class_00_00162_volume_sharp.mrc" |
| 13620 | | |
| 13621 | | Opened cryosparc_P4_J117_class_00_00162_volume_sharp.mrc as #13, grid size |
| 13622 | | 128,128,128, pixel 2.08, shown at level 0.163, step 1, values float32 |
| 13623 | | |
| 13624 | | > hide #!8 models |
| 13625 | | |
| 13626 | | > select add #13 |
| 13627 | | |
| 13628 | | 2 models selected |
| 13629 | | |
| 13630 | | > ui mousemode right "translate selected models" |
| 13631 | | |
| 13632 | | > view matrix models #13,1,0,0,6.2015,0,1,0,0.69431,0,0,1,-17.516 |
| 13633 | | |
| 13634 | | > ui mousemode right "rotate selected models" |
| 13635 | | |
| 13636 | | > view matrix models |
| 13637 | | > #13,-0.13264,-0.68512,-0.71625,337.99,0.0019176,-0.72281,0.69104,120.39,-0.99116,0.090284,0.097186,224.71 |
| 13638 | | |
| 13639 | | > view matrix models |
| 13640 | | > #13,0.0040424,0.12352,-0.99233,257.27,0.14964,-0.98124,-0.12153,245.92,-0.98873,-0.148,-0.02245,270.75 |
| 13641 | | |
| 13642 | | > select subtract #13 |
| 13643 | | |
| 13644 | | Nothing selected |
| 13645 | | |
| 13646 | | > show #!12 models |
| 13647 | | |
| 13648 | | > hide #!12 models |
| 13649 | | |
| 13650 | | > show #!11 models |
| 13651 | | |
| 13652 | | > hide #!11 models |
| 13653 | | |
| 13654 | | > show #!10 models |
| 13655 | | |
| 13656 | | > fitmap #13 inMap #10 |
| 13657 | | |
| 13658 | | Fit map cryosparc_P4_J117_class_00_00162_volume_sharp.mrc in map |
| 13659 | | cryosparc_P4_J242_class_02_00122_volume_sharp.mrc using 20961 points |
| 13660 | | correlation = 0.8109, correlation about mean = 0.2077, overlap = 1054 |
| 13661 | | steps = 92, shift = 12.7, angle = 18.1 degrees |
| 13662 | | |
| 13663 | | Position of cryosparc_P4_J117_class_00_00162_volume_sharp.mrc (#13) relative |
| 13664 | | to cryosparc_P4_J242_class_02_00122_volume_sharp.mrc (#10) coordinates: |
| 13665 | | Matrix rotation and translation |
| 13666 | | -0.98193657 -0.07856914 0.17212633 243.32837219 |
| 13667 | | 0.00195692 -0.91387700 -0.40598645 309.66348462 |
| 13668 | | 0.18920029 -0.39831610 0.89752300 41.87114917 |
| 13669 | | Axis 0.09277951 -0.20652448 0.97403265 |
| 13670 | | Axis point 116.38449758 161.85402651 0.00000000 |
| 13671 | | Rotation angle (degrees) 177.63092236 |
| 13672 | | Shift along axis -0.59333628 |
| 13673 | | |
| 13674 | | |
| 13675 | | > hide #!10 models |
| 13676 | | |
| 13677 | | > hide #!13 models |
| 13678 | | |
| 13679 | | > open "/Users/peiyuyu/Documents/Goethe |
| 13680 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 13681 | | > F_B1/cryosparc_P4_J117_class_01_00162_volume_sharp.mrc" |
| 13682 | | |
| 13683 | | Opened cryosparc_P4_J117_class_01_00162_volume_sharp.mrc as #14, grid size |
| 13684 | | 128,128,128, pixel 2.08, shown at level 0.187, step 1, values float32 |
| 13685 | | |
| 13686 | | > select add #14 |
| 13687 | | |
| 13688 | | 2 models selected |
| 13689 | | |
| 13690 | | > view matrix models |
| 13691 | | > #14,-0.39254,-0.77748,0.49135,234.79,-0.04345,-0.51796,-0.8543,320.26,0.91871,-0.3567,0.16954,27.288 |
| 13692 | | |
| 13693 | | > view matrix models |
| 13694 | | > #14,-0.061348,0.89979,0.432,-25.376,-0.288,-0.43036,0.85548,115.2,0.95566,-0.071931,0.28554,-30.976 |
| 13695 | | |
| 13696 | | > view matrix models |
| 13697 | | > #14,-0.046716,0.65995,-0.74985,161.94,-0.070464,0.74662,0.6615,-44.914,0.99642,0.08374,0.011624,-20.712 |
| 13698 | | |
| 13699 | | > select subtract #14 |
| 13700 | | |
| 13701 | | Nothing selected |
| 13702 | | |
| 13703 | | > show #!13 models |
| 13704 | | |
| 13705 | | > fitmap #14 inMap #13 |
| 13706 | | |
| 13707 | | Fit map cryosparc_P4_J117_class_01_00162_volume_sharp.mrc in map |
| 13708 | | cryosparc_P4_J117_class_00_00162_volume_sharp.mrc using 20968 points |
| 13709 | | correlation = 0.846, correlation about mean = 0.3465, overlap = 1110 |
| 13710 | | steps = 132, shift = 18.5, angle = 19.5 degrees |
| 13711 | | |
| 13712 | | Position of cryosparc_P4_J117_class_01_00162_volume_sharp.mrc (#14) relative |
| 13713 | | to cryosparc_P4_J117_class_00_00162_volume_sharp.mrc (#13) coordinates: |
| 13714 | | Matrix rotation and translation |
| 13715 | | -0.99955515 0.02982234 0.00035424 260.68209471 |
| 13716 | | -0.00628473 -0.19900464 -0.97997839 284.54003350 |
| 13717 | | -0.02915475 -0.97954468 0.19910354 244.04930876 |
| 13718 | | Axis 0.00930049 0.63278447 -0.77427212 |
| 13719 | | Axis point 133.98017190 240.03303746 0.00000000 |
| 13720 | | Rotation angle (degrees) 178.66392593 |
| 13721 | | Shift along axis -6.48359029 |
| 13722 | | |
| 13723 | | |
| 13724 | | > open "/Users/peiyuyu/Documents/Goethe |
| 13725 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 13726 | | > F_B1/cryosparc_P4_J117_class_02_00162_volume_sharp.mrc" |
| 13727 | | |
| 13728 | | Opened cryosparc_P4_J117_class_02_00162_volume_sharp.mrc as #15, grid size |
| 13729 | | 128,128,128, pixel 2.08, shown at level 0.251, step 1, values float32 |
| 13730 | | |
| 13731 | | > hide #!13 models |
| 13732 | | |
| 13733 | | > select add #14 |
| 13734 | | |
| 13735 | | 2 models selected |
| 13736 | | |
| 13737 | | > show #!12 models |
| 13738 | | |
| 13739 | | > select subtract #14 |
| 13740 | | |
| 13741 | | Nothing selected |
| 13742 | | |
| 13743 | | > hide #!14 models |
| 13744 | | |
| 13745 | | > select add #15 |
| 13746 | | |
| 13747 | | 2 models selected |
| 13748 | | |
| 13749 | | > view matrix models |
| 13750 | | > #15,-0.98221,0.18526,0.030552,219.51,0.18651,0.98144,0.044675,-26.394,-0.021708,0.049578,-0.99853,243.73 |
| 13751 | | |
| 13752 | | > ui mousemode right "translate selected models" |
| 13753 | | |
| 13754 | | > view matrix models |
| 13755 | | > #15,-0.98221,0.18526,0.030552,234.62,0.18651,0.98144,0.044675,-36.86,-0.021708,0.049578,-0.99853,271.78 |
| 13756 | | |
| 13757 | | > fitmap #15 inMap #12 |
| 13758 | | |
| 13759 | | Fit map cryosparc_P4_J117_class_02_00162_volume_sharp.mrc in map |
| 13760 | | cryosparc_P4_J242_class_04_00122_volume_sharp.mrc using 20967 points |
| 13761 | | correlation = 0.781, correlation about mean = 0.07908, overlap = 1573 |
| 13762 | | steps = 76, shift = 10.9, angle = 6.65 degrees |
| 13763 | | |
| 13764 | | Position of cryosparc_P4_J117_class_02_00162_volume_sharp.mrc (#15) relative |
| 13765 | | to cryosparc_P4_J242_class_04_00122_volume_sharp.mrc (#12) coordinates: |
| 13766 | | Matrix rotation and translation |
| 13767 | | 0.82536305 0.56130744 0.06090799 -57.36751075 |
| 13768 | | -0.56057744 0.82755504 -0.03009290 101.38226037 |
| 13769 | | -0.06729608 -0.00930608 0.99768964 1.14989876 |
| 13770 | | Axis 0.01840555 0.11351744 -0.99336550 |
| 13771 | | Axis point 134.55599767 144.37243816 0.00000000 |
| 13772 | | Rotation angle (degrees) 34.38068857 |
| 13773 | | Shift along axis 9.31050435 |
| 13774 | | |
| 13775 | | |
| 13776 | | > volume flip #15 |
| 13777 | | |
| 13778 | | Opened cryosparc_P4_J117_class_02_00162_volume_sharp.mrc z flip as #16, grid |
| 13779 | | size 128,128,128, pixel 2.08, shown at step 1, values float32 |
| 13780 | | |
| 13781 | | > close #15 |
| 13782 | | |
| 13783 | | > select add #16 |
| 13784 | | |
| 13785 | | 2 models selected |
| 13786 | | |
| 13787 | | > ui mousemode right "rotate selected models" |
| 13788 | | |
| 13789 | | > view matrix models |
| 13790 | | > #16,-0.81511,-0.57721,0.04929,312.41,0.52972,-0.77708,-0.33992,218.14,0.2345,-0.25096,0.93916,8.6526 |
| 13791 | | |
| 13792 | | > fitmap #15 inMap #12 |
| 13793 | | |
| 13794 | | No atoms or maps for #15 |
| 13795 | | |
| 13796 | | > fitmap #16 inMap #12 |
| 13797 | | |
| 13798 | | Fit map cryosparc_P4_J117_class_02_00162_volume_sharp.mrc z flip in map |
| 13799 | | cryosparc_P4_J242_class_04_00122_volume_sharp.mrc using 20967 points |
| 13800 | | correlation = 0.9777, correlation about mean = 0.7437, overlap = 2747 |
| 13801 | | steps = 108, shift = 14.1, angle = 17.5 degrees |
| 13802 | | |
| 13803 | | Position of cryosparc_P4_J117_class_02_00162_volume_sharp.mrc z flip (#16) |
| 13804 | | relative to cryosparc_P4_J242_class_04_00122_volume_sharp.mrc (#12) |
| 13805 | | coordinates: |
| 13806 | | Matrix rotation and translation |
| 13807 | | 0.94024990 -0.32652551 -0.09649458 65.60674301 |
| 13808 | | -0.33221344 -0.94188358 -0.04989541 307.74881291 |
| 13809 | | -0.07459454 0.07897095 -0.99408211 253.25354142 |
| 13810 | | Axis 0.98493295 -0.16738329 -0.04347315 |
| 13811 | | Axis point 0.00000000 155.23786381 133.19610246 |
| 13812 | | Rotation angle (degrees) 176.24909728 |
| 13813 | | Shift along axis 2.09650544 |
| 13814 | | |
| 13815 | | |
| 13816 | | > select subtract #16 |
| 13817 | | |
| 13818 | | Nothing selected |
| 13819 | | |
| 13820 | | > rename #16 CS_P4_J117_class_02_sharp_flip.mrc id #15 |
| 13821 | | |
| 13822 | | > save CS_P4_J117_class_02_sharp_flip.mrc #15 |
| 13823 | | |
| 13824 | | > open "/Users/peiyuyu/Documents/Goethe |
| 13825 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 13826 | | > F_B1/cryosparc_P4_J117_class_03_00162_volume_sharp.mrc" |
| 13827 | | |
| 13828 | | Opened cryosparc_P4_J117_class_03_00162_volume_sharp.mrc as #16, grid size |
| 13829 | | 128,128,128, pixel 2.08, shown at level 0.179, step 1, values float32 |
| 13830 | | |
| 13831 | | > hide #!12 models |
| 13832 | | |
| 13833 | | > hide #!15 models |
| 13834 | | |
| 13835 | | > select add #16 |
| 13836 | | |
| 13837 | | 2 models selected |
| 13838 | | |
| 13839 | | > show #!14 models |
| 13840 | | |
| 13841 | | > view matrix models |
| 13842 | | > #16,0.00021536,0.34795,0.93751,-37.63,0.50671,0.80821,-0.30007,0.053261,-0.86212,0.47511,-0.17613,183.13 |
| 13843 | | |
| 13844 | | > hide #!14 models |
| 13845 | | |
| 13846 | | > view matrix models |
| 13847 | | > #16,0.63015,0.10301,-0.76961,125.95,-0.74582,0.35606,-0.56301,261.02,0.21603,0.92877,0.3012,-81.743 |
| 13848 | | |
| 13849 | | > view matrix models |
| 13850 | | > #16,0.63277,-0.43368,-0.64151,190.37,0.75188,0.14602,0.64293,-47.242,-0.18515,-0.88916,0.41847,227.83 |
| 13851 | | |
| 13852 | | > show #!13 models |
| 13853 | | |
| 13854 | | > hide #!13 models |
| 13855 | | |
| 13856 | | > show #!12 models |
| 13857 | | |
| 13858 | | > hide #!12 models |
| 13859 | | |
| 13860 | | > show #!11 models |
| 13861 | | |
| 13862 | | > ui mousemode right "translate selected models" |
| 13863 | | |
| 13864 | | > view matrix models |
| 13865 | | > #16,0.63277,-0.43368,-0.64151,206.09,0.75188,0.14602,0.64293,-70.694,-0.18515,-0.88916,0.41847,249.05 |
| 13866 | | |
| 13867 | | > view matrix models |
| 13868 | | > #16,0.63277,-0.43368,-0.64151,204.5,0.75188,0.14602,0.64293,-75.196,-0.18515,-0.88916,0.41847,240.94 |
| 13869 | | |
| 13870 | | > ui mousemode right "rotate selected models" |
| 13871 | | |
| 13872 | | > view matrix models |
| 13873 | | > #16,-0.30976,-0.38252,-0.87047,346.06,0.94397,-0.2334,-0.23335,63.654,-0.1139,-0.89398,0.43339,230.67 |
| 13874 | | |
| 13875 | | > fitmap #16 inMap #11 |
| 13876 | | |
| 13877 | | Fit map cryosparc_P4_J117_class_03_00162_volume_sharp.mrc in map |
| 13878 | | cryosparc_P4_J242_class_03_00122_volume_sharp.mrc using 20958 points |
| 13879 | | correlation = 0.9023, correlation about mean = 0.3197, overlap = 1117 |
| 13880 | | steps = 184, shift = 6.54, angle = 53.8 degrees |
| 13881 | | |
| 13882 | | Position of cryosparc_P4_J117_class_03_00162_volume_sharp.mrc (#16) relative |
| 13883 | | to cryosparc_P4_J242_class_03_00122_volume_sharp.mrc (#11) coordinates: |
| 13884 | | Matrix rotation and translation |
| 13885 | | 0.41743274 -0.19488532 -0.88756388 222.32976103 |
| 13886 | | -0.90862481 -0.10271919 -0.40478358 322.57606660 |
| 13887 | | -0.01228347 0.97543246 -0.21995599 21.59894000 |
| 13888 | | Axis 0.77392148 -0.49079151 -0.40021149 |
| 13889 | | Axis point 0.00000000 222.40854643 197.20887777 |
| 13890 | | Rotation angle (degrees) 116.91198346 |
| 13891 | | Shift along axis 5.10403978 |
| 13892 | | |
| 13893 | | |
| 13894 | | > select subtract #16 |
| 13895 | | |
| 13896 | | Nothing selected |
| 13897 | | |
| 13898 | | > hide #!11 models |
| 13899 | | |
| 13900 | | > open "/Users/peiyuyu/Documents/Goethe |
| 13901 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 13902 | | > F_B1/cryosparc_P4_J117_class_04_00162_volume_sharp.mrc" |
| 13903 | | |
| 13904 | | Opened cryosparc_P4_J117_class_04_00162_volume_sharp.mrc as #17, grid size |
| 13905 | | 128,128,128, pixel 2.08, shown at level 0.206, step 1, values float32 |
| 13906 | | |
| 13907 | | > hide #!16 models |
| 13908 | | |
| 13909 | | > select add #17 |
| 13910 | | |
| 13911 | | 2 models selected |
| 13912 | | |
| 13913 | | > show #!13 models |
| 13914 | | |
| 13915 | | > hide #!13 models |
| 13916 | | |
| 13917 | | > show #!11 models |
| 13918 | | |
| 13919 | | > hide #!11 models |
| 13920 | | |
| 13921 | | > show #!10 models |
| 13922 | | |
| 13923 | | > hide #!17 models |
| 13924 | | |
| 13925 | | > show #!17 models |
| 13926 | | |
| 13927 | | > view matrix models |
| 13928 | | > #17,-0.0034658,-0.079884,-0.9968,294.42,-0.15421,0.98492,-0.078396,35.543,0.98803,0.15344,-0.015732,-30.795 |
| 13929 | | |
| 13930 | | > fitmap #17 inMap #10 |
| 13931 | | |
| 13932 | | Fit map cryosparc_P4_J117_class_04_00162_volume_sharp.mrc in map |
| 13933 | | cryosparc_P4_J242_class_02_00122_volume_sharp.mrc using 20958 points |
| 13934 | | correlation = 0.8416, correlation about mean = 0.3036, overlap = 1170 |
| 13935 | | steps = 148, shift = 13.9, angle = 32.2 degrees |
| 13936 | | |
| 13937 | | Position of cryosparc_P4_J117_class_04_00162_volume_sharp.mrc (#17) relative |
| 13938 | | to cryosparc_P4_J242_class_02_00122_volume_sharp.mrc (#10) coordinates: |
| 13939 | | Matrix rotation and translation |
| 13940 | | 0.73519379 0.67627298 0.04631360 -60.93633283 |
| 13941 | | -0.67522557 0.73664284 -0.03778583 134.15115598 |
| 13942 | | -0.05967011 -0.00349222 0.99821205 3.94076312 |
| 13943 | | Axis 0.02528875 0.07815439 -0.99662047 |
| 13944 | | Axis point 141.29888305 145.62167557 0.00000000 |
| 13945 | | Rotation angle (degrees) 42.69072745 |
| 13946 | | Shift along axis 5.01605257 |
| 13947 | | |
| 13948 | | |
| 13949 | | > select subtract #17 |
| 13950 | | |
| 13951 | | Nothing selected |
| 13952 | | |
| 13953 | | > hide #!10 models |
| 13954 | | |
| 13955 | | > show #!10 models |
| 13956 | | |
| 13957 | | > hide #!17 models |
| 13958 | | |
| 13959 | | > save "/Users/peiyuyu/Documents/Goethe |
| 13960 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 13961 | | > F_B1/20260501_SA-F_B1_workflow.cxs" |
| 13962 | | |
| 13963 | | > show #!13 models |
| 13964 | | |
| 13965 | | > hide #!10 models |
| 13966 | | |
| 13967 | | > color #13 #FCEDFF |
| 13968 | | |
| 13969 | | > hide #!13 models |
| 13970 | | |
| 13971 | | > show #!14 models |
| 13972 | | |
| 13973 | | > color #14 #FCEDFF |
| 13974 | | |
| 13975 | | > show #!15 models |
| 13976 | | |
| 13977 | | > hide #!14 models |
| 13978 | | |
| 13979 | | > hide #!15 models |
| 13980 | | |
| 13981 | | > show #!16 models |
| 13982 | | |
| 13983 | | > color #16 #FCEDFF |
| 13984 | | |
| 13985 | | > hide #!16 models |
| 13986 | | |
| 13987 | | > show #!17 models |
| 13988 | | |
| 13989 | | > color #17 #FCEDFF |
| 13990 | | |
| 13991 | | > show #!15 models |
| 13992 | | |
| 13993 | | > hide #!17 models |
| 13994 | | |
| 13995 | | > color #15 #99BFE5 |
| 13996 | | |
| 13997 | | > volume #15 level 0.3349 |
| 13998 | | |
| 13999 | | > ui mousemode right translate |
| 14000 | | |
| 14001 | | > view P1 |
| 14002 | | |
| 14003 | | [Repeated 1 time(s)] |
| 14004 | | |
| 14005 | | > save P4_J117_class_2_sharp.png supersample 3 transparentBackground true |
| 14006 | | |
| 14007 | | > hide #!15 models |
| 14008 | | |
| 14009 | | > show #!13 models |
| 14010 | | |
| 14011 | | > save P4_J117_class_0_sharp.png supersample 3 transparentBackground true |
| 14012 | | |
| 14013 | | > hide #!13 models |
| 14014 | | |
| 14015 | | > show #!14 models |
| 14016 | | |
| 14017 | | > save P4_J117_class_1_sharp.png supersample 3 transparentBackground true |
| 14018 | | |
| 14019 | | > hide #!14 models |
| 14020 | | |
| 14021 | | > show #!16 models |
| 14022 | | |
| 14023 | | > save P4_J117_class_3_sharp.png supersample 3 transparentBackground true |
| 14024 | | |
| 14025 | | > hide #!16 models |
| 14026 | | |
| 14027 | | > show #!17 models |
| 14028 | | |
| 14029 | | > save P4_J117_class_4_sharp.png supersample 3 transparentBackground true |
| 14030 | | |
| 14031 | | [Repeated 1 time(s)] |
| 14032 | | |
| 14033 | | > save "/Users/peiyuyu/Documents/Goethe |
| 14034 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 14035 | | > F_B1/20260501_SA-F_B1_workflow.cxs" |
| 14036 | | |
| 14037 | | > open "/Users/peiyuyu/Documents/Goethe |
| 14038 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 14039 | | > F_B1/cryosparc_P4_J120_002_volume_map_sharp.mrc" |
| 14040 | | |
| 14041 | | Opened cryosparc_P4_J120_002_volume_map_sharp.mrc as #18, grid size 80,80,80, |
| 14042 | | pixel 3.32, shown at level 1.08, step 1, values float32 |
| 14043 | | |
| 14044 | | > hide #!17 models |
| 14045 | | |
| 14046 | | > show #!15 models |
| 14047 | | |
| 14048 | | > select add #18 |
| 14049 | | |
| 14050 | | 2 models selected |
| 14051 | | |
| 14052 | | > ui mousemode right "rotate selected models" |
| 14053 | | |
| 14054 | | > view matrix models |
| 14055 | | > #18,0.9199,0.35329,0.17023,-57.439,0.37414,-0.92071,-0.11099,219.04,0.11752,0.16579,-0.97913,208.75 |
| 14056 | | |
| 14057 | | > ui mousemode right "translate selected models" |
| 14058 | | |
| 14059 | | > view matrix models |
| 14060 | | > #18,0.9199,0.35329,0.17023,-56.147,0.37414,-0.92071,-0.11099,218.08,0.11752,0.16579,-0.97913,234.23 |
| 14061 | | |
| 14062 | | > ui mousemode right "rotate selected models" |
| 14063 | | |
| 14064 | | > view matrix models |
| 14065 | | > #18,0.47537,0.87487,0.092902,-59.652,0.86989,-0.48319,0.099115,73.008,0.1316,0.033698,-0.99073,251.23 |
| 14066 | | |
| 14067 | | > volume flip #18 |
| 14068 | | |
| 14069 | | Opened cryosparc_P4_J120_002_volume_map_sharp.mrc z flip as #19, grid size |
| 14070 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 14071 | | |
| 14072 | | > select subtract #18 |
| 14073 | | |
| 14074 | | Nothing selected |
| 14075 | | |
| 14076 | | > close #18 |
| 14077 | | |
| 14078 | | > select add #19 |
| 14079 | | |
| 14080 | | 2 models selected |
| 14081 | | |
| 14082 | | > view matrix models |
| 14083 | | > #19,0.011836,0.9987,-0.049536,1.5471,-0.96239,0.024824,0.27053,210.58,0.27141,0.044471,0.96144,-38.44 |
| 14084 | | |
| 14085 | | > ui mousemode right "translate selected models" |
| 14086 | | |
| 14087 | | > view matrix models |
| 14088 | | > #19,0.011836,0.9987,-0.049536,5.1894,-0.96239,0.024824,0.27053,209.75,0.27141,0.044471,0.96144,-23.291 |
| 14089 | | |
| 14090 | | > fitmap #19 inMap #15 |
| 14091 | | |
| 14092 | | Fit map cryosparc_P4_J120_002_volume_map_sharp.mrc z flip in map |
| 14093 | | CS_P4_J117_class_02_sharp_flip.mrc using 5118 points |
| 14094 | | correlation = 0.9182, correlation about mean = 0.533, overlap = 3573 |
| 14095 | | steps = 76, shift = 9.96, angle = 10.7 degrees |
| 14096 | | |
| 14097 | | Position of cryosparc_P4_J120_002_volume_map_sharp.mrc z flip (#19) relative |
| 14098 | | to CS_P4_J117_class_02_sharp_flip.mrc (#15) coordinates: |
| 14099 | | Matrix rotation and translation |
| 14100 | | -0.48058823 -0.86458262 0.14673736 288.72558748 |
| 14101 | | 0.85464108 -0.49926017 -0.14257599 104.76974006 |
| 14102 | | 0.19652884 0.05688743 0.97884638 -28.97188121 |
| 14103 | | Axis 0.11519876 -0.02875674 0.99292613 |
| 14104 | | Axis point 115.59153027 135.80137987 0.00000000 |
| 14105 | | Rotation angle (degrees) 120.03315219 |
| 14106 | | Shift along axis 1.48105620 |
| 14107 | | |
| 14108 | | |
| 14109 | | > ui mousemode right "rotate selected models" |
| 14110 | | |
| 14111 | | > view matrix models |
| 14112 | | > #19,0.89305,-0.32489,-0.3113,106.99,0.34183,0.93976,-0.00013805,-25.175,0.29259,-0.10629,0.95031,-3.3753 |
| 14113 | | |
| 14114 | | > ui mousemode right "translate selected models" |
| 14115 | | |
| 14116 | | > view matrix models |
| 14117 | | > #19,0.89305,-0.32489,-0.3113,96.178,0.34183,0.93976,-0.00013805,-42.263,0.29259,-0.10629,0.95031,-5.1551 |
| 14118 | | |
| 14119 | | > fitmap #19 inMap #15 |
| 14120 | | |
| 14121 | | Fit map cryosparc_P4_J120_002_volume_map_sharp.mrc z flip in map |
| 14122 | | CS_P4_J117_class_02_sharp_flip.mrc using 5118 points |
| 14123 | | correlation = 0.9187, correlation about mean = 0.5356, overlap = 3578 |
| 14124 | | steps = 76, shift = 8.31, angle = 8.52 degrees |
| 14125 | | |
| 14126 | | Position of cryosparc_P4_J120_002_volume_map_sharp.mrc z flip (#19) relative |
| 14127 | | to CS_P4_J117_class_02_sharp_flip.mrc (#15) coordinates: |
| 14128 | | Matrix rotation and translation |
| 14129 | | -0.47913034 0.85546358 0.19651003 54.96243137 |
| 14130 | | -0.86530880 -0.49790427 0.05772364 303.37608678 |
| 14131 | | 0.14722365 -0.14238471 0.97880120 3.73022055 |
| 14132 | | Axis -0.11546470 0.02843878 -0.99290440 |
| 14133 | | Axis point 115.58296139 135.79077530 0.00000000 |
| 14134 | | Rotation angle (degrees) 119.94157891 |
| 14135 | | Shift along axis -1.42232844 |
| 14136 | | |
| 14137 | | |
| 14138 | | > hide #!19 models |
| 14139 | | |
| 14140 | | > hide #!15 models |
| 14141 | | |
| 14142 | | > show #!15 models |
| 14143 | | |
| 14144 | | > hide #!15 models |
| 14145 | | |
| 14146 | | > show #!19 models |
| 14147 | | |
| 14148 | | > select subtract #19 |
| 14149 | | |
| 14150 | | Nothing selected |
| 14151 | | |
| 14152 | | > show #!15 models |
| 14153 | | |
| 14154 | | > hide #!15 models |
| 14155 | | |
| 14156 | | > rename #19 CS_P4_J120_sharp_flip id #18 |
| 14157 | | |
| 14158 | | > save CS_P4_J120_sharp_flip.mrc #18 |
| 14159 | | |
| 14160 | | > volume #18 level 1.185 |
| 14161 | | |
| 14162 | | > volume #18 level 1.639 |
| 14163 | | |
| 14164 | | > volume #18 step 2 |
| 14165 | | |
| 14166 | | > volume #18 step 1 |
| 14167 | | |
| 14168 | | > show #!15 models |
| 14169 | | |
| 14170 | | > volume #18 level 1.559 |
| 14171 | | |
| 14172 | | > hide #!15 models |
| 14173 | | |
| 14174 | | > volume #18 level 1.292 |
| 14175 | | |
| 14176 | | > view P1 |
| 14177 | | |
| 14178 | | > color #18 #99BFE5 |
| 14179 | | |
| 14180 | | > show #!17 models |
| 14181 | | |
| 14182 | | > hide #!17 models |
| 14183 | | |
| 14184 | | > hide #!18 models |
| 14185 | | |
| 14186 | | > show #!18 models |
| 14187 | | |
| 14188 | | > save P4_J120_NU_sharp.png supersample 3 transparentBackground true |
| 14189 | | |
| 14190 | | > save "/Users/peiyuyu/Documents/Goethe |
| 14191 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 14192 | | > F_B1/20260501_SA-F_B1_workflow.cxs" |
| 14193 | | |
| 14194 | | > open "/Users/peiyuyu/Documents/Goethe |
| 14195 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 14196 | | > F_B1/cryosparc_P4_J169_class_00_00182_volume_sharp.mrc" |
| 14197 | | |
| 14198 | | Opened cryosparc_P4_J169_class_00_00182_volume_sharp.mrc as #19, grid size |
| 14199 | | 128,128,128, pixel 2.08, shown at level 0.175, step 1, values float32 |
| 14200 | | |
| 14201 | | > select add #19 |
| 14202 | | |
| 14203 | | 2 models selected |
| 14204 | | |
| 14205 | | > hide #!18 models |
| 14206 | | |
| 14207 | | > show #!18 models |
| 14208 | | |
| 14209 | | > ui mousemode right "rotate selected models" |
| 14210 | | |
| 14211 | | > ui mousemode right "translate selected models" |
| 14212 | | |
| 14213 | | > view matrix models #19,1,0,0,-7.1681,0,1,0,-1.6755,0,0,1,29.407 |
| 14214 | | |
| 14215 | | > fitmap #19 inMap #18 |
| 14216 | | |
| 14217 | | Fit map cryosparc_P4_J169_class_00_00182_volume_sharp.mrc in map |
| 14218 | | CS_P4_J120_sharp_flip.mrc using 20970 points |
| 14219 | | correlation = 0.3797, correlation about mean = 0.05313, overlap = 2374 |
| 14220 | | steps = 192, shift = 6.4, angle = 17.5 degrees |
| 14221 | | |
| 14222 | | Position of cryosparc_P4_J169_class_00_00182_volume_sharp.mrc (#19) relative |
| 14223 | | to CS_P4_J120_sharp_flip.mrc (#18) coordinates: |
| 14224 | | Matrix rotation and translation |
| 14225 | | 0.64594502 0.66965380 0.36649532 -93.21942227 |
| 14226 | | -0.65111130 0.73392063 -0.19342850 149.32350272 |
| 14227 | | -0.39850860 -0.11368507 0.91009153 102.27587247 |
| 14228 | | Axis 0.05217441 0.50052557 -0.86414813 |
| 14229 | | Axis point 153.99219258 219.18137479 0.00000000 |
| 14230 | | Rotation angle (degrees) 49.83592965 |
| 14231 | | Shift along axis -18.50494005 |
| 14232 | | |
| 14233 | | |
| 14234 | | > select subtract #19 |
| 14235 | | |
| 14236 | | Nothing selected |
| 14237 | | |
| 14238 | | > hide #!18 models |
| 14239 | | |
| 14240 | | > open "/Users/peiyuyu/Documents/Goethe |
| 14241 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 14242 | | > F_B1/cryosparc_P4_J169_class_01_00182_volume_sharp.mrc" |
| 14243 | | |
| 14244 | | Opened cryosparc_P4_J169_class_01_00182_volume_sharp.mrc as #20, grid size |
| 14245 | | 128,128,128, pixel 2.08, shown at level 0.189, step 1, values float32 |
| 14246 | | |
| 14247 | | > hide #!19 models |
| 14248 | | |
| 14249 | | > show #!17 models |
| 14250 | | |
| 14251 | | > select add #20 |
| 14252 | | |
| 14253 | | 2 models selected |
| 14254 | | |
| 14255 | | > ui mousemode right "rotate selected models" |
| 14256 | | |
| 14257 | | > view matrix models |
| 14258 | | > #20,0.023382,0.1088,0.99379,-27.517,0.050205,0.99268,-0.10986,9.6432,-0.99847,0.052462,0.017749,251.64 |
| 14259 | | |
| 14260 | | > hide #!17 models |
| 14261 | | |
| 14262 | | > show #!16 models |
| 14263 | | |
| 14264 | | > hide #!16 models |
| 14265 | | |
| 14266 | | > show #!15 models |
| 14267 | | |
| 14268 | | > hide #!15 models |
| 14269 | | |
| 14270 | | > show #!14 models |
| 14271 | | |
| 14272 | | > ui mousemode right "translate selected models" |
| 14273 | | |
| 14274 | | > view matrix models |
| 14275 | | > #20,0.023382,0.1088,0.99379,-22.964,0.050205,0.99268,-0.10986,7.1023,-0.99847,0.052462,0.017749,242.74 |
| 14276 | | |
| 14277 | | > ui mousemode right "rotate selected models" |
| 14278 | | |
| 14279 | | > view matrix models |
| 14280 | | > #20,-0.029978,-0.71994,-0.69339,335.92,-0.21064,-0.67357,0.70847,177,-0.9771,0.16729,-0.13145,243.19 |
| 14281 | | |
| 14282 | | > ui mousemode right "translate selected models" |
| 14283 | | |
| 14284 | | > view matrix models |
| 14285 | | > #20,-0.029978,-0.71994,-0.69339,328.25,-0.21064,-0.67357,0.70847,170.9,-0.9771,0.16729,-0.13145,240.41 |
| 14286 | | |
| 14287 | | > fitmap #20 inMap #14 |
| 14288 | | |
| 14289 | | Fit map cryosparc_P4_J169_class_01_00182_volume_sharp.mrc in map |
| 14290 | | cryosparc_P4_J117_class_01_00162_volume_sharp.mrc using 20954 points |
| 14291 | | correlation = 0.8829, correlation about mean = 0.4124, overlap = 1200 |
| 14292 | | steps = 104, shift = 14, angle = 14 degrees |
| 14293 | | |
| 14294 | | Position of cryosparc_P4_J169_class_01_00182_volume_sharp.mrc (#20) relative |
| 14295 | | to cryosparc_P4_J117_class_01_00162_volume_sharp.mrc (#14) coordinates: |
| 14296 | | Matrix rotation and translation |
| 14297 | | -0.94773816 0.28655181 -0.14028702 243.79600144 |
| 14298 | | -0.24891252 -0.93914826 -0.23673424 326.23627964 |
| 14299 | | -0.19958693 -0.18944287 0.96139298 60.20896934 |
| 14300 | | Axis 0.08744550 0.10965027 -0.99011621 |
| 14301 | | Axis point 147.27291182 149.47535150 0.00000000 |
| 14302 | | Rotation angle (degrees) 164.31164420 |
| 14303 | | Shift along axis -2.52311906 |
| 14304 | | |
| 14305 | | |
| 14306 | | > select subtract #20 |
| 14307 | | |
| 14308 | | Nothing selected |
| 14309 | | |
| 14310 | | > hide #!14 models |
| 14311 | | |
| 14312 | | > open "/Users/peiyuyu/Documents/Goethe |
| 14313 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 14314 | | > F_B1/cryosparc_P4_J169_class_02_00182_volume_sharp.mrc" |
| 14315 | | |
| 14316 | | Opened cryosparc_P4_J169_class_02_00182_volume_sharp.mrc as #21, grid size |
| 14317 | | 128,128,128, pixel 2.08, shown at level 0.216, step 1, values float32 |
| 14318 | | |
| 14319 | | > hide #!20 models |
| 14320 | | |
| 14321 | | > select add #21 |
| 14322 | | |
| 14323 | | 2 models selected |
| 14324 | | |
| 14325 | | > view matrix models #21,1,0,0,-13.073,0,1,0,8.3491,0,0,1,8.6967 |
| 14326 | | |
| 14327 | | > ui mousemode right "rotate selected models" |
| 14328 | | |
| 14329 | | > view matrix models |
| 14330 | | > #21,-0.06365,0.67952,0.73089,-53.58,-0.2928,0.68743,-0.66461,177.89,-0.95405,-0.25631,0.15521,285.48 |
| 14331 | | |
| 14332 | | > view matrix models |
| 14333 | | > #21,0.10048,-0.44353,0.89061,41.096,0.13793,-0.88029,-0.45395,284.46,0.98533,0.16846,-0.027275,-4.3073 |
| 14334 | | |
| 14335 | | > fitmap #21 inMap #20 |
| 14336 | | |
| 14337 | | Fit map cryosparc_P4_J169_class_02_00182_volume_sharp.mrc in map |
| 14338 | | cryosparc_P4_J169_class_01_00182_volume_sharp.mrc using 20962 points |
| 14339 | | correlation = 0.7829, correlation about mean = 0.1539, overlap = 900.6 |
| 14340 | | steps = 188, shift = 21.5, angle = 32 degrees |
| 14341 | | |
| 14342 | | Position of cryosparc_P4_J169_class_02_00182_volume_sharp.mrc (#21) relative |
| 14343 | | to cryosparc_P4_J169_class_01_00182_volume_sharp.mrc (#20) coordinates: |
| 14344 | | Matrix rotation and translation |
| 14345 | | -0.75680258 0.56772680 0.32393846 109.96194933 |
| 14346 | | 0.37589927 0.78345603 -0.49487007 56.85693302 |
| 14347 | | -0.53474254 -0.25275071 -0.80632963 338.09031391 |
| 14348 | | Axis 0.26532092 0.94096581 -0.21020979 |
| 14349 | | Axis point 90.65236627 0.00000000 161.68382083 |
| 14350 | | Rotation angle (degrees) 152.85290905 |
| 14351 | | Shift along axis 11.60574300 |
| 14352 | | |
| 14353 | | |
| 14354 | | > show #!20 models |
| 14355 | | |
| 14356 | | > select subtract #21 |
| 14357 | | |
| 14358 | | Nothing selected |
| 14359 | | |
| 14360 | | > select add #21 |
| 14361 | | |
| 14362 | | 2 models selected |
| 14363 | | |
| 14364 | | > ui mousemode right "translate selected models" |
| 14365 | | |
| 14366 | | > view matrix models |
| 14367 | | > #21,0.31625,-0.43913,0.84092,24.723,-0.35094,-0.87769,-0.32634,336.73,0.88138,-0.19191,-0.43168,94.219 |
| 14368 | | |
| 14369 | | > ui mousemode right "rotate selected models" |
| 14370 | | |
| 14371 | | > view matrix models |
| 14372 | | > #21,0.23561,0.79493,-0.55908,75.399,0.21977,0.51679,0.82742,-71.321,0.94667,-0.31782,-0.052938,48.925 |
| 14373 | | |
| 14374 | | > ui mousemode right "translate selected models" |
| 14375 | | |
| 14376 | | > view matrix models |
| 14377 | | > #21,0.23561,0.79493,-0.55908,69.861,0.21977,0.51679,0.82742,-70.077,0.94667,-0.31782,-0.052938,40.098 |
| 14378 | | |
| 14379 | | > fitmap #21 inMap #20 |
| 14380 | | |
| 14381 | | Fit map cryosparc_P4_J169_class_02_00182_volume_sharp.mrc in map |
| 14382 | | cryosparc_P4_J169_class_01_00182_volume_sharp.mrc using 20962 points |
| 14383 | | correlation = 0.8883, correlation about mean = 0.4082, overlap = 1398 |
| 14384 | | steps = 80, shift = 8.95, angle = 4.79 degrees |
| 14385 | | |
| 14386 | | Position of cryosparc_P4_J169_class_02_00182_volume_sharp.mrc (#21) relative |
| 14387 | | to cryosparc_P4_J169_class_01_00182_volume_sharp.mrc (#20) coordinates: |
| 14388 | | Matrix rotation and translation |
| 14389 | | -0.99875153 -0.02706229 -0.04198798 281.31180443 |
| 14390 | | 0.03395891 -0.98426911 -0.17338138 286.61926959 |
| 14391 | | -0.03663538 -0.17459079 0.98395929 28.58358251 |
| 14392 | | Axis -0.01973976 -0.08736459 0.99598081 |
| 14393 | | Axis point 138.73615561 146.72655614 0.00000000 |
| 14394 | | Rotation angle (degrees) 178.24454217 |
| 14395 | | Shift along axis -2.12470384 |
| 14396 | | |
| 14397 | | |
| 14398 | | > select subtract #21 |
| 14399 | | |
| 14400 | | Nothing selected |
| 14401 | | |
| 14402 | | > hide #!20 models |
| 14403 | | |
| 14404 | | > hide #!21 models |
| 14405 | | |
| 14406 | | > show #!21 models |
| 14407 | | |
| 14408 | | > show #!20 models |
| 14409 | | |
| 14410 | | > hide #!21 models |
| 14411 | | |
| 14412 | | > show #!21 models |
| 14413 | | |
| 14414 | | > open "/Users/peiyuyu/Documents/Goethe |
| 14415 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 14416 | | > F_B1/cryosparc_P4_J169_class_03_00182_volume_sharp.mrc" |
| 14417 | | |
| 14418 | | Opened cryosparc_P4_J169_class_03_00182_volume_sharp.mrc as #22, grid size |
| 14419 | | 128,128,128, pixel 2.08, shown at level 0.248, step 1, values float32 |
| 14420 | | |
| 14421 | | > hide #!20 models |
| 14422 | | |
| 14423 | | > hide #!21 models |
| 14424 | | |
| 14425 | | > select add #22 |
| 14426 | | |
| 14427 | | 2 models selected |
| 14428 | | |
| 14429 | | > show #!18 models |
| 14430 | | |
| 14431 | | > ui mousemode right "rotate selected models" |
| 14432 | | |
| 14433 | | > view matrix models |
| 14434 | | > #22,0.80286,-0.54835,0.23395,74.113,-0.54307,-0.83458,-0.092472,336.96,0.24595,-0.052807,-0.96784,209.56 |
| 14435 | | |
| 14436 | | > view matrix models |
| 14437 | | > #22,0.23802,0.97115,0.01476,-30.605,0.97056,-0.2384,0.03436,32.801,0.036887,0.0061467,-0.9993,233.87 |
| 14438 | | |
| 14439 | | > ui mousemode right "translate selected models" |
| 14440 | | |
| 14441 | | > view matrix models |
| 14442 | | > #22,0.23802,0.97115,0.01476,-35.135,0.97056,-0.2384,0.03436,31.843,0.036887,0.0061467,-0.9993,265.72 |
| 14443 | | |
| 14444 | | > view matrix models |
| 14445 | | > #22,0.23802,0.97115,0.01476,-30.091,0.97056,-0.2384,0.03436,32.303,0.036887,0.0061467,-0.9993,265.71 |
| 14446 | | |
| 14447 | | > select subtract #22 |
| 14448 | | |
| 14449 | | Nothing selected |
| 14450 | | |
| 14451 | | > hide #!18 models |
| 14452 | | |
| 14453 | | > volume flip #22 |
| 14454 | | |
| 14455 | | Opened cryosparc_P4_J169_class_03_00182_volume_sharp.mrc z flip as #23, grid |
| 14456 | | size 128,128,128, pixel 2.08, shown at step 1, values float32 |
| 14457 | | |
| 14458 | | > close #22 |
| 14459 | | |
| 14460 | | > show #!21 models |
| 14461 | | |
| 14462 | | > hide #!21 models |
| 14463 | | |
| 14464 | | > show #!18 models |
| 14465 | | |
| 14466 | | > select add #23 |
| 14467 | | |
| 14468 | | 2 models selected |
| 14469 | | |
| 14470 | | > view matrix models |
| 14471 | | > #23,0.23802,0.97115,0.01476,-34.192,0.97056,-0.2384,0.03436,27.412,0.036887,0.0061467,-0.9993,278.73 |
| 14472 | | |
| 14473 | | > ui mousemode right "rotate selected models" |
| 14474 | | |
| 14475 | | > view matrix models |
| 14476 | | > #23,-0.23315,-0.92003,-0.31494,337.04,0.93397,-0.30205,0.19095,18.61,-0.27081,-0.24962,0.92971,78.293 |
| 14477 | | |
| 14478 | | > ui mousemode right "translate selected models" |
| 14479 | | |
| 14480 | | > view matrix models |
| 14481 | | > #23,-0.23315,-0.92003,-0.31494,335.86,0.93397,-0.30205,0.19095,18.866,-0.27081,-0.24962,0.92971,88.616 |
| 14482 | | |
| 14483 | | > fitmap #23 inMap #18 |
| 14484 | | |
| 14485 | | Fit map cryosparc_P4_J169_class_03_00182_volume_sharp.mrc z flip in map |
| 14486 | | CS_P4_J120_sharp_flip.mrc using 20952 points |
| 14487 | | correlation = 0.9077, correlation about mean = 0.6695, overlap = 1.202e+04 |
| 14488 | | steps = 60, shift = 4.34, angle = 10.2 degrees |
| 14489 | | |
| 14490 | | Position of cryosparc_P4_J169_class_03_00182_volume_sharp.mrc z flip (#23) |
| 14491 | | relative to CS_P4_J120_sharp_flip.mrc (#18) coordinates: |
| 14492 | | Matrix rotation and translation |
| 14493 | | 0.17479617 -0.97573598 0.13185442 219.49728105 |
| 14494 | | 0.98179238 0.18284123 0.05150543 -32.00811477 |
| 14495 | | -0.07436413 0.12045072 0.98993010 -9.54926019 |
| 14496 | | Axis 0.03500529 0.10470244 0.99388733 |
| 14497 | | Axis point 128.78581299 116.22335978 0.00000000 |
| 14498 | | Rotation angle (degrees) 79.99211268 |
| 14499 | | Shift along axis -5.15865059 |
| 14500 | | |
| 14501 | | |
| 14502 | | > select subtract #23 |
| 14503 | | |
| 14504 | | Nothing selected |
| 14505 | | |
| 14506 | | > hide #!18 models |
| 14507 | | |
| 14508 | | > rename #23 CS_P4_J169_class_03_sharp_flip id #22 |
| 14509 | | |
| 14510 | | > save CS_P4_J169_class_03_sharp_flip.mrc #22 |
| 14511 | | |
| 14512 | | > show #!21 models |
| 14513 | | |
| 14514 | | > hide #!21 models |
| 14515 | | |
| 14516 | | > show #!18 models |
| 14517 | | |
| 14518 | | > hide #!18 models |
| 14519 | | |
| 14520 | | > open "/Users/peiyuyu/Documents/Goethe |
| 14521 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 14522 | | > F_B1/cryosparc_P4_J169_class_04_00182_volume_sharp.mrc" |
| 14523 | | |
| 14524 | | Opened cryosparc_P4_J169_class_04_00182_volume_sharp.mrc as #23, grid size |
| 14525 | | 128,128,128, pixel 2.08, shown at level 0.171, step 1, values float32 |
| 14526 | | |
| 14527 | | > hide #!22 models |
| 14528 | | |
| 14529 | | > show #!21 models |
| 14530 | | |
| 14531 | | > select add #23 |
| 14532 | | |
| 14533 | | 2 models selected |
| 14534 | | |
| 14535 | | > view matrix models #23,1,0,0,62.54,0,1,0,-0.93224,0,0,1,-9.0713 |
| 14536 | | |
| 14537 | | > ui mousemode right "rotate selected models" |
| 14538 | | |
| 14539 | | > view matrix models |
| 14540 | | > #23,0.11131,-0.99321,-0.033862,304.62,-0.95598,-0.11633,0.2694,223.09,-0.27151,0.0023841,-0.96243,297.1 |
| 14541 | | |
| 14542 | | > view matrix models |
| 14543 | | > #23,0.14785,0.8725,0.4657,-3.2346,-0.24604,0.48852,-0.83714,210.48,-0.95792,0.0091875,0.2869,210.23 |
| 14544 | | |
| 14545 | | > ui mousemode right "translate selected models" |
| 14546 | | |
| 14547 | | > view matrix models |
| 14548 | | > #23,0.14785,0.8725,0.4657,-72.613,-0.24604,0.48852,-0.83714,211.81,-0.95792,0.0091875,0.2869,205.44 |
| 14549 | | |
| 14550 | | > ui mousemode right "rotate selected models" |
| 14551 | | |
| 14552 | | > view matrix models |
| 14553 | | > #23,-0.12372,0.72067,0.68215,-49.094,0.065146,0.69184,-0.7191,130.45,-0.99018,-0.044529,-0.13254,274.4 |
| 14554 | | |
| 14555 | | > ui mousemode right "translate selected models" |
| 14556 | | |
| 14557 | | > view matrix models |
| 14558 | | > #23,-0.12372,0.72067,0.68215,-52.059,0.065146,0.69184,-0.7191,130.58,-0.99018,-0.044529,-0.13254,270.2 |
| 14559 | | |
| 14560 | | > fitmap #23 inMap #21 |
| 14561 | | |
| 14562 | | Fit map cryosparc_P4_J169_class_04_00182_volume_sharp.mrc in map |
| 14563 | | cryosparc_P4_J169_class_02_00182_volume_sharp.mrc using 20956 points |
| 14564 | | correlation = 0.8996, correlation about mean = 0.4033, overlap = 1373 |
| 14565 | | steps = 168, shift = 14.7, angle = 39.5 degrees |
| 14566 | | |
| 14567 | | Position of cryosparc_P4_J169_class_04_00182_volume_sharp.mrc (#23) relative |
| 14568 | | to cryosparc_P4_J169_class_02_00182_volume_sharp.mrc (#21) coordinates: |
| 14569 | | Matrix rotation and translation |
| 14570 | | -0.99961182 0.01552465 -0.02313457 267.26750037 |
| 14571 | | 0.02397017 0.90245504 -0.43011668 70.06567251 |
| 14572 | | 0.01420050 -0.43050425 -0.90247684 305.57661651 |
| 14573 | | Axis -0.01012464 -0.97530665 0.22062281 |
| 14574 | | Axis point 131.76679627 0.00000000 162.02424501 |
| 14575 | | Rotation angle (degrees) 178.90328205 |
| 14576 | | Shift along axis -3.62433410 |
| 14577 | | |
| 14578 | | |
| 14579 | | > select subtract #23 |
| 14580 | | |
| 14581 | | Nothing selected |
| 14582 | | |
| 14583 | | > hide #!23 models |
| 14584 | | |
| 14585 | | > hide #!21 models |
| 14586 | | |
| 14587 | | > show #!23 models |
| 14588 | | |
| 14589 | | > volume #23 level 0.1996 |
| 14590 | | |
| 14591 | | > hide #!23 models |
| 14592 | | |
| 14593 | | > show #!22 models |
| 14594 | | |
| 14595 | | > volume #22 level 0.2715 |
| 14596 | | |
| 14597 | | > hide #!22 models |
| 14598 | | |
| 14599 | | > show #!21 models |
| 14600 | | |
| 14601 | | > volume #21 level 0.2481 |
| 14602 | | |
| 14603 | | > hide #!21 models |
| 14604 | | |
| 14605 | | > show #!20 models |
| 14606 | | |
| 14607 | | > volume #20 level 0.198 |
| 14608 | | |
| 14609 | | > hide #!20 models |
| 14610 | | |
| 14611 | | > show #!19 models |
| 14612 | | |
| 14613 | | > volume #19 level 0.1357 |
| 14614 | | |
| 14615 | | > volume #19 level 0.1565 |
| 14616 | | |
| 14617 | | > color #9 #FCEDFF |
| 14618 | | |
| 14619 | | > show #!9 models |
| 14620 | | |
| 14621 | | > hide #!9 models |
| 14622 | | |
| 14623 | | > color #19 #FCEDFF |
| 14624 | | |
| 14625 | | > save "/Users/peiyuyu/Documents/Goethe |
| 14626 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 14627 | | > F_B1/20260501_SA-F_B1_workflow.cxs" |
| 14628 | | |
| 14629 | | > hide #!19 models |
| 14630 | | |
| 14631 | | > show #!20 models |
| 14632 | | |
| 14633 | | > color #20 #FCEDFF |
| 14634 | | |
| 14635 | | > hide #!20 models |
| 14636 | | |
| 14637 | | > show #!21 models |
| 14638 | | |
| 14639 | | > show #!20 models |
| 14640 | | |
| 14641 | | > hide #!20 models |
| 14642 | | |
| 14643 | | > color #21 #FCEDFF |
| 14644 | | |
| 14645 | | > hide #!21 models |
| 14646 | | |
| 14647 | | > show #!22 models |
| 14648 | | |
| 14649 | | > show #!23 models |
| 14650 | | |
| 14651 | | > hide #!22 models |
| 14652 | | |
| 14653 | | > color #23 #FCEDFF |
| 14654 | | |
| 14655 | | > color #22 #99BFE5 |
| 14656 | | |
| 14657 | | > show #!22 models |
| 14658 | | |
| 14659 | | > hide #!23 models |
| 14660 | | |
| 14661 | | > hide #!22 models |
| 14662 | | |
| 14663 | | > show #!22 models |
| 14664 | | |
| 14665 | | > save "/Users/peiyuyu/Documents/Goethe |
| 14666 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 14667 | | > F_B1/20260501_SA-F_B1_workflow.cxs" |
| 14668 | | |
| 14669 | | > view P1 |
| 14670 | | |
| 14671 | | > save P4_J169_class_3_sharp.png supersample 3 transparentBackground true |
| 14672 | | |
| 14673 | | > hide #!22 models |
| 14674 | | |
| 14675 | | > show #!19 models |
| 14676 | | |
| 14677 | | > save P4_J169_class_0_sharp.png supersample 3 transparentBackground true |
| 14678 | | |
| 14679 | | > hide #!19 models |
| 14680 | | |
| 14681 | | > show #!20 models |
| 14682 | | |
| 14683 | | > save P4_J169_class_1_sharp.png supersample 3 transparentBackground true |
| 14684 | | |
| 14685 | | > hide #!20 models |
| 14686 | | |
| 14687 | | > show #!21 models |
| 14688 | | |
| 14689 | | > save P4_J169_class_2_sharp.png supersample 3 transparentBackground true |
| 14690 | | |
| 14691 | | > hide #!21 models |
| 14692 | | |
| 14693 | | > show #!22 models |
| 14694 | | |
| 14695 | | > show #!23 models |
| 14696 | | |
| 14697 | | > hide #!22 models |
| 14698 | | |
| 14699 | | > save P4_J169_class_4_sharp.png supersample 3 transparentBackground true |
| 14700 | | |
| 14701 | | > save "/Users/peiyuyu/Documents/Goethe |
| 14702 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 14703 | | > F_B1/20260501_SA-F_B1_workflow.cxs" |
| 14704 | | |
| 14705 | | > open "/Users/peiyuyu/Documents/Goethe |
| 14706 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 14707 | | > F_B1/cryosparc_P4_J176_002_volume_map_sharp.mrc" |
| 14708 | | |
| 14709 | | Opened cryosparc_P4_J176_002_volume_map_sharp.mrc as #24, grid size 80,80,80, |
| 14710 | | pixel 3.32, shown at level 1.05, step 1, values float32 |
| 14711 | | |
| 14712 | | > show #!22 models |
| 14713 | | |
| 14714 | | > hide #!23 models |
| 14715 | | |
| 14716 | | > select add #24 |
| 14717 | | |
| 14718 | | 2 models selected |
| 14719 | | |
| 14720 | | > ui mousemode right "rotate selected models" |
| 14721 | | |
| 14722 | | > view matrix models |
| 14723 | | > #24,0.60544,-0.7944,-0.048687,168.77,-0.79404,-0.60707,0.031193,325.29,-0.054337,0.019774,-0.99833,244.32 |
| 14724 | | |
| 14725 | | > view matrix models |
| 14726 | | > #24,0.59955,-0.80023,-0.012763,166.07,-0.79939,-0.59954,0.03913,324.05,-0.038965,-0.013258,-0.99915,246.8 |
| 14727 | | |
| 14728 | | > view matrix models |
| 14729 | | > #24,0.55506,-0.74882,-0.36218,207.08,-0.8209,-0.56341,-0.093219,337.96,-0.13425,0.34906,-0.92744,201.93 |
| 14730 | | |
| 14731 | | > view matrix models |
| 14732 | | > #24,0.18767,0.98161,-0.034938,-17.516,0.95411,-0.19063,-0.23093,58.314,-0.23335,0.010004,-0.97234,267.28 |
| 14733 | | |
| 14734 | | > volume flip #24 |
| 14735 | | |
| 14736 | | Opened cryosparc_P4_J176_002_volume_map_sharp.mrc z flip as #25, grid size |
| 14737 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 14738 | | |
| 14739 | | > close #24 |
| 14740 | | |
| 14741 | | > select add #25 |
| 14742 | | |
| 14743 | | 2 models selected |
| 14744 | | |
| 14745 | | > view matrix models |
| 14746 | | > #25,-0.63444,0.77032,-0.064025,129.07,-0.77136,-0.63629,-0.011971,326.31,-0.04996,0.041792,0.99788,-43.633 |
| 14747 | | |
| 14748 | | > ui mousemode right "translate selected models" |
| 14749 | | |
| 14750 | | > view matrix models |
| 14751 | | > #25,-0.63444,0.77032,-0.064025,133.77,-0.77136,-0.63629,-0.011971,326.45,-0.04996,0.041792,0.99788,5.2846 |
| 14752 | | |
| 14753 | | > fitmap #25 inMap #22 |
| 14754 | | |
| 14755 | | Fit map cryosparc_P4_J176_002_volume_map_sharp.mrc z flip in map |
| 14756 | | CS_P4_J169_class_03_sharp_flip.mrc using 5115 points |
| 14757 | | correlation = 0.927, correlation about mean = 0.5943, overlap = 3935 |
| 14758 | | steps = 92, shift = 11.4, angle = 9.34 degrees |
| 14759 | | |
| 14760 | | Position of cryosparc_P4_J176_002_volume_map_sharp.mrc z flip (#25) relative |
| 14761 | | to CS_P4_J169_class_03_sharp_flip.mrc (#22) coordinates: |
| 14762 | | Matrix rotation and translation |
| 14763 | | -0.48979714 -0.86290735 -0.12445753 333.02911722 |
| 14764 | | 0.86312907 -0.50006428 0.07031310 74.16708713 |
| 14765 | | -0.12291046 -0.07298376 0.98973047 29.10111268 |
| 14766 | | Axis -0.08273609 -0.00089324 0.99657109 |
| 14767 | | Axis point 146.24021491 133.58927656 0.00000000 |
| 14768 | | Rotation angle (degrees) 120.00433179 |
| 14769 | | Shift along axis 1.38155071 |
| 14770 | | |
| 14771 | | |
| 14772 | | > select subtract #25 |
| 14773 | | |
| 14774 | | Nothing selected |
| 14775 | | |
| 14776 | | > rename #25 CS_P4_J176_NU_sharp_flip id #24 |
| 14777 | | |
| 14778 | | > save CS_P4_J176_NU_sharp_flip.mrc #24 |
| 14779 | | |
| 14780 | | > hide #!22 models |
| 14781 | | |
| 14782 | | > volume #24 level 1.757 |
| 14783 | | |
| 14784 | | > volume #24 level 1.364 |
| 14785 | | |
| 14786 | | > color #24 #99BFE5 |
| 14787 | | |
| 14788 | | > volume #24 level 1.6 |
| 14789 | | |
| 14790 | | > save "/Users/peiyuyu/Documents/Goethe |
| 14791 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 14792 | | > F_B1/20260501_SA-F_B1_workflow.cxs" |
| 14793 | | |
| 14794 | | > view P1 |
| 14795 | | |
| 14796 | | > save CS_P4_J176_NU_sharp.png supersample 3 transparentBackground true |
| 14797 | | |
| 14798 | | > open "/Users/peiyuyu/Documents/Goethe University/19. Model Building/2. AcrF |
| 14799 | | > Project/1. Apo-form/LG-F_Apo/Map/cryosparc_P10_J41_007_volume_map_sharp.mrc" |
| 14800 | | |
| 14801 | | Opened cryosparc_P10_J41_007_volume_map_sharp.mrc as #25, grid size |
| 14802 | | 320,320,320, pixel 0.831, shown at level 0.123, step 2, values float32 |
| 14803 | | |
| 14804 | | > hide #!24 models |
| 14805 | | |
| 14806 | | > show #!22 models |
| 14807 | | |
| 14808 | | > hide #!22 models |
| 14809 | | |
| 14810 | | > volume #25 level 0.2164 |
| 14811 | | |
| 14812 | | > save CS_P10_J41_NU_sharp_flip.mrc #25 |
| 14813 | | |
| 14814 | | > close #25 |
| 14815 | | |
| 14816 | | > show #!24 models |
| 14817 | | |
| 14818 | | > save "/Users/peiyuyu/Documents/Goethe |
| 14819 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 14820 | | > F_B1/20260501_SA-F_B1_workflow1.cxs" |
| 14821 | | |
| 14822 | | > open "/Users/peiyuyu/Documents/Goethe |
| 14823 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 14824 | | > F_B1/cryosparc_P4_J255_002_volume_map_sharp.mrc" |
| 14825 | | |
| 14826 | | Opened cryosparc_P4_J255_002_volume_map_sharp.mrc as #25, grid size 80,80,80, |
| 14827 | | pixel 3.32, shown at level 0.649, step 1, values float32 |
| 14828 | | |
| 14829 | | > hide #!24 models |
| 14830 | | |
| 14831 | | > volume flip #25 |
| 14832 | | |
| 14833 | | Opened cryosparc_P4_J255_002_volume_map_sharp.mrc z flip as #26, grid size |
| 14834 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 14835 | | |
| 14836 | | > close #25 |
| 14837 | | |
| 14838 | | > rename CS_P4_J255_sharp_flip id #25 |
| 14839 | | |
| 14840 | | Missing or invalid "models" argument: invalid models specifier |
| 14841 | | |
| 14842 | | > rename #26 CS_P4_J255_sharp_flip id #25 |
| 14843 | | |
| 14844 | | > save CS_P4_J255_sharp_flip.mrc #25 |
| 14845 | | |
| 14846 | | > show #!21 models |
| 14847 | | |
| 14848 | | > hide #!21 models |
| 14849 | | |
| 14850 | | > show #!24 models |
| 14851 | | |
| 14852 | | > fitmap #25 inMap #24 |
| 14853 | | |
| 14854 | | Fit map CS_P4_J255_sharp_flip.mrc in map CS_P4_J176_NU_sharp_flip.mrc using |
| 14855 | | 5116 points |
| 14856 | | correlation = 0.3392, correlation about mean = 0.06206, overlap = 2168 |
| 14857 | | steps = 64, shift = 8.32, angle = 5.45 degrees |
| 14858 | | |
| 14859 | | Position of CS_P4_J255_sharp_flip.mrc (#25) relative to |
| 14860 | | CS_P4_J176_NU_sharp_flip.mrc (#24) coordinates: |
| 14861 | | Matrix rotation and translation |
| 14862 | | -0.61720035 -0.75989860 -0.20400451 353.05950420 |
| 14863 | | 0.73150715 -0.64968488 0.20689815 96.93395715 |
| 14864 | | -0.28976026 -0.02153315 0.95685700 43.12583281 |
| 14865 | | Axis -0.15115555 0.05674554 0.98687990 |
| 14866 | | Axis point 157.34041998 128.70985965 0.00000000 |
| 14867 | | Rotation angle (degrees) 130.92072050 |
| 14868 | | Shift along axis -5.30631665 |
| 14869 | | |
| 14870 | | |
| 14871 | | > ui mousemode right "rotate selected models" |
| 14872 | | |
| 14873 | | > select add #25 |
| 14874 | | |
| 14875 | | 2 models selected |
| 14876 | | |
| 14877 | | > view matrix models |
| 14878 | | > #25,-0.47042,-0.83357,-0.28961,363.38,0.84754,-0.51818,0.11478,76.044,-0.24575,-0.19146,0.95024,72.926 |
| 14879 | | |
| 14880 | | > ui mousemode right "translate selected models" |
| 14881 | | |
| 14882 | | > view matrix models |
| 14883 | | > #25,-0.47042,-0.83357,-0.28961,355.09,0.84754,-0.51818,0.11478,66.81,-0.24575,-0.19146,0.95024,74.95 |
| 14884 | | |
| 14885 | | > view matrix models |
| 14886 | | > #25,-0.47042,-0.83357,-0.28961,355.15,0.84754,-0.51818,0.11478,66.803,-0.24575,-0.19146,0.95024,74.561 |
| 14887 | | |
| 14888 | | > fitmap #25 inMap #24 |
| 14889 | | |
| 14890 | | Fit map CS_P4_J255_sharp_flip.mrc in map CS_P4_J176_NU_sharp_flip.mrc using |
| 14891 | | 5116 points |
| 14892 | | correlation = 0.8943, correlation about mean = 0.6059, overlap = 8709 |
| 14893 | | steps = 84, shift = 8.95, angle = 14.5 degrees |
| 14894 | | |
| 14895 | | Position of CS_P4_J255_sharp_flip.mrc (#25) relative to |
| 14896 | | CS_P4_J176_NU_sharp_flip.mrc (#24) coordinates: |
| 14897 | | Matrix rotation and translation |
| 14898 | | -0.48869161 0.86324945 -0.12641554 102.63693670 |
| 14899 | | -0.86157381 -0.50031742 -0.08586638 328.42188396 |
| 14900 | | -0.13737200 0.06695414 0.98825405 11.50507804 |
| 14901 | | Axis 0.08825319 0.00632731 -0.99607798 |
| 14902 | | Axis point 146.96136624 134.50878130 0.00000000 |
| 14903 | | Rotation angle (degrees) 120.02497766 |
| 14904 | | Shift along axis -0.32389146 |
| 14905 | | |
| 14906 | | |
| 14907 | | > select subtract #25 |
| 14908 | | |
| 14909 | | Nothing selected |
| 14910 | | |
| 14911 | | > save CS_P4_J255_sharp_flip.mrc #25 |
| 14912 | | |
| 14913 | | > open "/Users/peiyuyu/Documents/Goethe |
| 14914 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 14915 | | > F_B1/cryosparc_P4_J256_003_volume_map_sharp.mrc" |
| 14916 | | |
| 14917 | | Opened cryosparc_P4_J256_003_volume_map_sharp.mrc as #26, grid size 80,80,80, |
| 14918 | | pixel 3.32, shown at level 1.01, step 1, values float32 |
| 14919 | | |
| 14920 | | > hide #!25 models |
| 14921 | | |
| 14922 | | > hide #!24 models |
| 14923 | | |
| 14924 | | > select add #26 |
| 14925 | | |
| 14926 | | 2 models selected |
| 14927 | | |
| 14928 | | > ui mousemode right "rotate selected models" |
| 14929 | | |
| 14930 | | > view matrix models |
| 14931 | | > #26,-0.89385,-0.39986,0.20284,290.33,-0.34063,0.89978,0.2727,28.49,-0.29155,0.17466,-0.94047,242.77 |
| 14932 | | |
| 14933 | | > select subtract #26 |
| 14934 | | |
| 14935 | | Nothing selected |
| 14936 | | |
| 14937 | | > show #!25 models |
| 14938 | | |
| 14939 | | > fitmap #26 inMap #25 |
| 14940 | | |
| 14941 | | Fit map cryosparc_P4_J256_003_volume_map_sharp.mrc in map |
| 14942 | | CS_P4_J255_sharp_flip.mrc using 5120 points |
| 14943 | | correlation = 0.5232, correlation about mean = 0.0553, overlap = 3214 |
| 14944 | | steps = 120, shift = 25.3, angle = 15.3 degrees |
| 14945 | | |
| 14946 | | Position of cryosparc_P4_J256_003_volume_map_sharp.mrc (#26) relative to |
| 14947 | | CS_P4_J255_sharp_flip.mrc (#25) coordinates: |
| 14948 | | Matrix rotation and translation |
| 14949 | | -0.15531756 0.84438274 0.51273214 -19.64184672 |
| 14950 | | 0.98713928 0.15254692 0.04780678 -29.86548711 |
| 14951 | | -0.03784849 0.51356326 -0.85721658 167.46829582 |
| 14952 | | Axis 0.63355180 0.74893504 0.19418658 |
| 14953 | | Axis point 24.16142457 0.00000000 89.91884511 |
| 14954 | | Rotation angle (degrees) 158.43381860 |
| 14955 | | Shift along axis -2.29134077 |
| 14956 | | |
| 14957 | | |
| 14958 | | > select add #26 |
| 14959 | | |
| 14960 | | 2 models selected |
| 14961 | | |
| 14962 | | > ui mousemode right "translate selected models" |
| 14963 | | |
| 14964 | | > view matrix models |
| 14965 | | > #26,-0.87857,-0.47692,-0.025855,314.85,-0.44818,0.80449,0.38978,38.161,-0.16509,0.35404,-0.92054,233.61 |
| 14966 | | |
| 14967 | | > ui mousemode right "rotate selected models" |
| 14968 | | |
| 14969 | | > view matrix models |
| 14970 | | > #26,-0.93629,-0.25688,0.23952,260.92,-0.23661,0.96532,0.11035,19.606,-0.25956,0.046645,-0.9646,294.56 |
| 14971 | | |
| 14972 | | > ui mousemode right "translate selected models" |
| 14973 | | |
| 14974 | | > view matrix models |
| 14975 | | > #26,-0.93629,-0.25688,0.23952,186.31,-0.23661,0.96532,0.11035,26.555,-0.25956,0.046645,-0.9646,285.53 |
| 14976 | | |
| 14977 | | > select subtract #26 |
| 14978 | | |
| 14979 | | Nothing selected |
| 14980 | | |
| 14981 | | > volume flip #26 |
| 14982 | | |
| 14983 | | Opened cryosparc_P4_J256_003_volume_map_sharp.mrc z flip as #27, grid size |
| 14984 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 14985 | | |
| 14986 | | > close #26 |
| 14987 | | |
| 14988 | | > select add #27 |
| 14989 | | |
| 14990 | | 2 models selected |
| 14991 | | |
| 14992 | | > ui mousemode right "rotate selected models" |
| 14993 | | |
| 14994 | | > view matrix models |
| 14995 | | > #27,-0.91261,-0.40844,0.017647,236.5,0.22697,-0.47029,0.85283,55.691,-0.34003,0.78231,0.5219,-22.328 |
| 14996 | | |
| 14997 | | > view matrix models |
| 14998 | | > #27,-0.9144,-0.40478,0.0045882,238.13,0.22235,-0.49276,0.84128,61.136,-0.33827,0.77029,0.54058,-23.608 |
| 14999 | | |
| 15000 | | > view matrix models |
| 15001 | | > #27,-0.50015,0.79795,-0.33634,63.369,-0.80597,-0.57099,-0.15615,357.35,-0.31664,0.19298,0.92871,-2.4372 |
| 15002 | | |
| 15003 | | > ui mousemode right "translate selected models" |
| 15004 | | |
| 15005 | | > view matrix models |
| 15006 | | > #27,-0.50015,0.79795,-0.33634,139.66,-0.80597,-0.57099,-0.15615,346.55,-0.31664,0.19298,0.92871,33.757 |
| 15007 | | |
| 15008 | | > fitmap #27 inMap #25 |
| 15009 | | |
| 15010 | | Fit map cryosparc_P4_J256_003_volume_map_sharp.mrc z flip in map |
| 15011 | | CS_P4_J255_sharp_flip.mrc using 5120 points |
| 15012 | | correlation = 0.5523, correlation about mean = 0.08213, overlap = 3668 |
| 15013 | | steps = 72, shift = 2.85, angle = 5.49 degrees |
| 15014 | | |
| 15015 | | Position of cryosparc_P4_J256_003_volume_map_sharp.mrc z flip (#27) relative |
| 15016 | | to CS_P4_J255_sharp_flip.mrc (#25) coordinates: |
| 15017 | | Matrix rotation and translation |
| 15018 | | -0.50078193 -0.83716343 -0.21994286 353.70374510 |
| 15019 | | 0.81104417 -0.54259583 0.21862553 68.19134780 |
| 15020 | | -0.30236538 -0.06889966 0.95069870 54.62167416 |
| 15021 | | Axis -0.17164375 0.04920372 0.98392958 |
| 15022 | | Axis point 162.79794553 129.91438640 0.00000000 |
| 15023 | | Rotation angle (degrees) 123.11624986 |
| 15024 | | Shift along axis -3.61188964 |
| 15025 | | |
| 15026 | | |
| 15027 | | > view matrix models |
| 15028 | | > #27,-0.56613,0.75777,-0.32448,155.66,-0.74832,-0.63753,-0.18323,351.66,-0.34571,0.13908,0.92798,47.028 |
| 15029 | | |
| 15030 | | > ui mousemode right "rotate selected models" |
| 15031 | | |
| 15032 | | > view matrix models |
| 15033 | | > #27,0.96274,0.27031,-0.0085132,-30.789,-0.27044,0.96237,-0.026732,40.459,0.00096683,0.028039,0.99961,4.8234 |
| 15034 | | |
| 15035 | | > fitmap #27 inMap #25 |
| 15036 | | |
| 15037 | | Fit map cryosparc_P4_J256_003_volume_map_sharp.mrc z flip in map |
| 15038 | | CS_P4_J255_sharp_flip.mrc using 5120 points |
| 15039 | | correlation = 0.9111, correlation about mean = 0.5876, overlap = 9195 |
| 15040 | | steps = 60, shift = 4.49, angle = 4.9 degrees |
| 15041 | | |
| 15042 | | Position of cryosparc_P4_J256_003_volume_map_sharp.mrc z flip (#27) relative |
| 15043 | | to CS_P4_J255_sharp_flip.mrc (#25) coordinates: |
| 15044 | | Matrix rotation and translation |
| 15045 | | -0.48877401 0.86149397 -0.13757950 104.57166396 |
| 15046 | | -0.86119668 -0.50165763 -0.08173068 327.97179743 |
| 15047 | | -0.13942829 0.07853517 0.98711295 10.19538079 |
| 15048 | | Axis 0.09263223 0.00106859 -0.99569982 |
| 15049 | | Axis point 147.63593381 133.73777093 0.00000000 |
| 15050 | | Rotation angle (degrees) 120.10984192 |
| 15051 | | Shift along axis -0.11436514 |
| 15052 | | |
| 15053 | | |
| 15054 | | > select subtract #27 |
| 15055 | | |
| 15056 | | Nothing selected |
| 15057 | | |
| 15058 | | > hide #!25 models |
| 15059 | | |
| 15060 | | > rename #27 CS_P4_J256_sharp_flip id #26 |
| 15061 | | |
| 15062 | | > save CS_P4_J256_sharp_flip.mrc #26 |
| 15063 | | |
| 15064 | | > show #!25 models |
| 15065 | | |
| 15066 | | > hide #!25 models |
| 15067 | | |
| 15068 | | > hide #!26 models |
| 15069 | | |
| 15070 | | > open "/Users/peiyuyu/Documents/Goethe |
| 15071 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 15072 | | > F_B1/cryosparc_P4_J257_002_volume_map_sharp.mrc" |
| 15073 | | |
| 15074 | | Opened cryosparc_P4_J257_002_volume_map_sharp.mrc as #27, grid size 80,80,80, |
| 15075 | | pixel 3.32, shown at level 0.915, step 1, values float32 |
| 15076 | | |
| 15077 | | > select add #27 |
| 15078 | | |
| 15079 | | 2 models selected |
| 15080 | | |
| 15081 | | > show #!26 models |
| 15082 | | |
| 15083 | | > view matrix models |
| 15084 | | > #27,0.43348,0.88304,0.17982,-64.533,0.89699,-0.44198,0.0081618,72.887,0.086685,0.15776,-0.98367,205.49 |
| 15085 | | |
| 15086 | | > hide #!26 models |
| 15087 | | |
| 15088 | | > select subtract #27 |
| 15089 | | |
| 15090 | | Nothing selected |
| 15091 | | |
| 15092 | | > volume flip #27 |
| 15093 | | |
| 15094 | | Opened cryosparc_P4_J257_002_volume_map_sharp.mrc z flip as #28, grid size |
| 15095 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 15096 | | |
| 15097 | | > close #27 |
| 15098 | | |
| 15099 | | > select add #28 |
| 15100 | | |
| 15101 | | 2 models selected |
| 15102 | | |
| 15103 | | > show #!26 models |
| 15104 | | |
| 15105 | | > view matrix models |
| 15106 | | > #28,0.0384,0.0015757,0.99926,-5.8546,0.92609,-0.37568,-0.034996,65.933,0.37535,0.92675,-0.015886,-77.078 |
| 15107 | | |
| 15108 | | > view matrix models |
| 15109 | | > #28,0.75378,0.65514,-0.051086,-44.642,-0.54582,0.58092,-0.60384,218.55,-0.36592,0.48304,0.79547,-29.499 |
| 15110 | | |
| 15111 | | > view matrix models |
| 15112 | | > #28,0.93053,0.32568,-0.16747,-7.3436,-0.34314,0.93516,-0.087963,68.823,0.12796,0.13932,0.98195,-76.969 |
| 15113 | | |
| 15114 | | > ui mousemode right "translate selected models" |
| 15115 | | |
| 15116 | | > view matrix models |
| 15117 | | > #28,0.93053,0.32568,-0.16747,-12.031,-0.34314,0.93516,-0.087963,69.72,0.12796,0.13932,0.98195,-23.264 |
| 15118 | | |
| 15119 | | > select subtract #28 |
| 15120 | | |
| 15121 | | Nothing selected |
| 15122 | | |
| 15123 | | > fitmap #28 inMap #26 |
| 15124 | | |
| 15125 | | Fit map cryosparc_P4_J257_002_volume_map_sharp.mrc z flip in map |
| 15126 | | CS_P4_J256_sharp_flip.mrc using 5113 points |
| 15127 | | correlation = 0.961, correlation about mean = 0.8355, overlap = 1.813e+04 |
| 15128 | | steps = 76, shift = 3.44, angle = 10.7 degrees |
| 15129 | | |
| 15130 | | Position of cryosparc_P4_J257_002_volume_map_sharp.mrc z flip (#28) relative |
| 15131 | | to CS_P4_J256_sharp_flip.mrc (#26) coordinates: |
| 15132 | | Matrix rotation and translation |
| 15133 | | 0.99983973 0.00266136 0.01770477 -3.60430289 |
| 15134 | | -0.00275366 0.99998275 0.00519130 -0.72993632 |
| 15135 | | -0.01769065 -0.00523922 0.99982979 3.00234542 |
| 15136 | | Axis -0.27967225 0.94905354 -0.14519233 |
| 15137 | | Axis point 166.73187411 0.00000000 206.99324341 |
| 15138 | | Rotation angle (degrees) 1.06849905 |
| 15139 | | Shift along axis -0.12064266 |
| 15140 | | |
| 15141 | | |
| 15142 | | > rename #28 CS_P4_J257_sharp_flip id #27 |
| 15143 | | |
| 15144 | | > save CS_P4_J257_sharp_flip.mrc #27 |
| 15145 | | |
| 15146 | | > hide #!26 models |
| 15147 | | |
| 15148 | | > hide #!27 models |
| 15149 | | |
| 15150 | | > open "/Users/peiyuyu/Documents/Goethe |
| 15151 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 15152 | | > F_B1/cryosparc_P4_J258_002_volume_map_sharp.mrc" |
| 15153 | | |
| 15154 | | Opened cryosparc_P4_J258_002_volume_map_sharp.mrc as #28, grid size 80,80,80, |
| 15155 | | pixel 3.32, shown at level 0.533, step 1, values float32 |
| 15156 | | |
| 15157 | | > select add #28 |
| 15158 | | |
| 15159 | | 2 models selected |
| 15160 | | |
| 15161 | | > show #!27 models |
| 15162 | | |
| 15163 | | > view matrix models #28,1,0,0,2.9293,0,1,0,12.799,0,0,1,30.196 |
| 15164 | | |
| 15165 | | > ui mousemode right "rotate selected models" |
| 15166 | | |
| 15167 | | > view matrix models |
| 15168 | | > #28,-0.94564,-0.32466,0.018849,316.84,-0.32175,0.94244,0.090998,54.708,-0.047307,0.079987,-0.99567,265.03 |
| 15169 | | |
| 15170 | | > select up |
| 15171 | | |
| 15172 | | 56 models selected |
| 15173 | | |
| 15174 | | > select up |
| 15175 | | |
| 15176 | | 56 models selected |
| 15177 | | |
| 15178 | | > select down |
| 15179 | | |
| 15180 | | 2 models selected |
| 15181 | | |
| 15182 | | > select down |
| 15183 | | |
| 15184 | | 2 models selected |
| 15185 | | |
| 15186 | | > select subtract #28 |
| 15187 | | |
| 15188 | | Nothing selected |
| 15189 | | |
| 15190 | | > hide #!27 models |
| 15191 | | |
| 15192 | | > volume flip #28 |
| 15193 | | |
| 15194 | | Opened cryosparc_P4_J258_002_volume_map_sharp.mrc z flip as #29, grid size |
| 15195 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 15196 | | |
| 15197 | | > close #28 |
| 15198 | | |
| 15199 | | > select add #29 |
| 15200 | | |
| 15201 | | 2 models selected |
| 15202 | | |
| 15203 | | > view matrix models |
| 15204 | | > #29,0.95822,0.27409,-0.081793,-16.892,-0.27702,0.96049,-0.026814,62.796,0.071212,0.048352,0.99629,-31.428 |
| 15205 | | |
| 15206 | | > show #!27 models |
| 15207 | | |
| 15208 | | > select subtract #29 |
| 15209 | | |
| 15210 | | Nothing selected |
| 15211 | | |
| 15212 | | > fitmap #29 inMap #27 |
| 15213 | | |
| 15214 | | Fit map cryosparc_P4_J258_002_volume_map_sharp.mrc z flip in map |
| 15215 | | CS_P4_J257_sharp_flip.mrc using 5117 points |
| 15216 | | correlation = 0.4043, correlation about mean = 0.1105, overlap = 1145 |
| 15217 | | steps = 104, shift = 9.56, angle = 9.45 degrees |
| 15218 | | |
| 15219 | | Position of cryosparc_P4_J258_002_volume_map_sharp.mrc z flip (#29) relative |
| 15220 | | to CS_P4_J257_sharp_flip.mrc (#27) coordinates: |
| 15221 | | Matrix rotation and translation |
| 15222 | | 0.98218261 0.18784329 -0.00567361 -27.82492616 |
| 15223 | | -0.18685004 0.97932905 0.07747035 28.59524537 |
| 15224 | | 0.02010861 -0.07502992 0.99697851 -24.36927466 |
| 15225 | | Axis -0.37621012 -0.06360338 -0.92434872 |
| 15226 | | Axis point 152.56947582 100.16313614 0.00000000 |
| 15227 | | Rotation angle (degrees) 11.69370116 |
| 15228 | | Shift along axis 31.17497259 |
| 15229 | | |
| 15230 | | |
| 15231 | | > select add #29 |
| 15232 | | |
| 15233 | | 2 models selected |
| 15234 | | |
| 15235 | | > ui mousemode right "translate selected models" |
| 15236 | | |
| 15237 | | > view matrix models |
| 15238 | | > #29,0.91472,0.40193,-0.041674,-52.044,-0.39883,0.91458,0.066882,60.074,0.064997,-0.044558,0.99689,0.70588 |
| 15239 | | |
| 15240 | | > view matrix models |
| 15241 | | > #29,0.91472,0.40193,-0.041674,-39.705,-0.39883,0.91458,0.066882,54.066,0.064997,-0.044558,0.99689,6.2725 |
| 15242 | | |
| 15243 | | > fitmap #29 inMap #27 |
| 15244 | | |
| 15245 | | Fit map cryosparc_P4_J258_002_volume_map_sharp.mrc z flip in map |
| 15246 | | CS_P4_J257_sharp_flip.mrc using 5117 points |
| 15247 | | correlation = 0.8335, correlation about mean = 0.5457, overlap = 5135 |
| 15248 | | steps = 64, shift = 1.52, angle = 12.1 degrees |
| 15249 | | |
| 15250 | | Position of cryosparc_P4_J258_002_volume_map_sharp.mrc z flip (#29) relative |
| 15251 | | to CS_P4_J257_sharp_flip.mrc (#27) coordinates: |
| 15252 | | Matrix rotation and translation |
| 15253 | | 0.99996433 -0.00752131 -0.00383843 1.74823892 |
| 15254 | | 0.00752090 0.99997170 -0.00012121 -0.88867123 |
| 15255 | | 0.00383923 0.00009234 0.99999262 -0.68815928 |
| 15256 | | Axis 0.01264381 -0.45457780 0.89061729 |
| 15257 | | Axis point 130.32470586 232.13443622 0.00000000 |
| 15258 | | Rotation angle (degrees) 0.48385831 |
| 15259 | | Shift along axis -0.18681194 |
| 15260 | | |
| 15261 | | |
| 15262 | | > select subtract #29 |
| 15263 | | |
| 15264 | | Nothing selected |
| 15265 | | |
| 15266 | | > rename #29 CS_P4_J258_sharp_flip id #28 |
| 15267 | | |
| 15268 | | > save CS_P4_J258_sharp_flip.mrc #28 |
| 15269 | | |
| 15270 | | > hide #!27 models |
| 15271 | | |
| 15272 | | > hide #!28 models |
| 15273 | | |
| 15274 | | > open "/Users/peiyuyu/Documents/Goethe |
| 15275 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 15276 | | > F_B1/cryosparc_P4_J259_002_volume_map_sharp.mrc" |
| 15277 | | |
| 15278 | | Opened cryosparc_P4_J259_002_volume_map_sharp.mrc as #29, grid size 80,80,80, |
| 15279 | | pixel 3.32, shown at level 0.567, step 1, values float32 |
| 15280 | | |
| 15281 | | > select add #29 |
| 15282 | | |
| 15283 | | 2 models selected |
| 15284 | | |
| 15285 | | > show #!28 models |
| 15286 | | |
| 15287 | | > view matrix models #29,1,0,0,-4.0284,0,1,0,3.9973,0,0,1,26.374 |
| 15288 | | |
| 15289 | | > ui mousemode right "rotate selected models" |
| 15290 | | |
| 15291 | | > view matrix models |
| 15292 | | > #29,0.37373,0.91624,0.14434,-63.019,0.92743,-0.36674,-0.073343,74.867,-0.014266,0.16128,-0.98681,258.72 |
| 15293 | | |
| 15294 | | > view matrix models |
| 15295 | | > #29,0.2129,0.93735,0.27578,-60.698,0.97539,-0.1873,-0.11636,49.108,-0.057419,0.29377,-0.95415,242.23 |
| 15296 | | |
| 15297 | | > hide #!28 models |
| 15298 | | |
| 15299 | | > select subtract #29 |
| 15300 | | |
| 15301 | | Nothing selected |
| 15302 | | |
| 15303 | | > volume flip #29 |
| 15304 | | |
| 15305 | | Opened cryosparc_P4_J259_002_volume_map_sharp.mrc z flip as #30, grid size |
| 15306 | | 80,80,80, pixel 3.32, shown at step 1, values float32 |
| 15307 | | |
| 15308 | | > close #29 |
| 15309 | | |
| 15310 | | > select add #30 |
| 15311 | | |
| 15312 | | 2 models selected |
| 15313 | | |
| 15314 | | > show #!28 models |
| 15315 | | |
| 15316 | | > view matrix models |
| 15317 | | > #30,0.90176,0.31537,-0.29559,8.3185,-0.32745,0.94483,0.0091223,54.046,0.28216,0.088565,0.95527,-34.506 |
| 15318 | | |
| 15319 | | > select subtract #30 |
| 15320 | | |
| 15321 | | Nothing selected |
| 15322 | | |
| 15323 | | > fitmap #30 inMap #28 |
| 15324 | | |
| 15325 | | Fit map cryosparc_P4_J259_002_volume_map_sharp.mrc z flip in map |
| 15326 | | CS_P4_J258_sharp_flip.mrc using 5112 points |
| 15327 | | correlation = 0.9666, correlation about mean = 0.6628, overlap = 3020 |
| 15328 | | steps = 80, shift = 5.99, angle = 15.1 degrees |
| 15329 | | |
| 15330 | | Position of cryosparc_P4_J259_002_volume_map_sharp.mrc z flip (#30) relative |
| 15331 | | to CS_P4_J258_sharp_flip.mrc (#28) coordinates: |
| 15332 | | Matrix rotation and translation |
| 15333 | | 0.99996699 0.00262374 -0.00768921 0.94371527 |
| 15334 | | -0.00261765 0.99999625 0.00080251 0.08556771 |
| 15335 | | 0.00769129 -0.00078235 0.99997011 -0.47262490 |
| 15336 | | Axis -0.09707497 -0.94207663 -0.32104217 |
| 15337 | | Axis point 62.77947672 0.00000000 122.20423531 |
| 15338 | | Rotation angle (degrees) 0.46771540 |
| 15339 | | Shift along axis -0.02048995 |
| 15340 | | |
| 15341 | | |
| 15342 | | > rename #30 CS_P4_J259_sharp_flip id #29 |
| 15343 | | |
| 15344 | | > save CS_P4_J259_sharp_flip.mrc #29 |
| 15345 | | |
| 15346 | | > save "/Users/peiyuyu/Documents/Goethe |
| 15347 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 15348 | | > F_B1/20260502_SA-F_B1_workflow.cxs" |
| 15349 | | |
| 15350 | | > hide #!29 models |
| 15351 | | |
| 15352 | | > hide #!28 models |
| 15353 | | |
| 15354 | | > show #!25 models |
| 15355 | | |
| 15356 | | > color #25 #FCEDFF |
| 15357 | | |
| 15358 | | > hide #!25 models |
| 15359 | | |
| 15360 | | > show #!28 models |
| 15361 | | |
| 15362 | | > color #28 #FCEDFF |
| 15363 | | |
| 15364 | | > hide #!28 models |
| 15365 | | |
| 15366 | | > show #!29 models |
| 15367 | | |
| 15368 | | > color #29 #FCEDFF |
| 15369 | | |
| 15370 | | > hide #!29 models |
| 15371 | | |
| 15372 | | > show #!29 models |
| 15373 | | |
| 15374 | | > show #!26 models |
| 15375 | | |
| 15376 | | > hide #!29 models |
| 15377 | | |
| 15378 | | > color #26 #99BFE5 |
| 15379 | | |
| 15380 | | > hide #!26 models |
| 15381 | | |
| 15382 | | > show #!27 models |
| 15383 | | |
| 15384 | | > color #27 #99BFE5 |
| 15385 | | |
| 15386 | | > view P1 |
| 15387 | | |
| 15388 | | > hide #!27 models |
| 15389 | | |
| 15390 | | > show #!25 models |
| 15391 | | |
| 15392 | | > ui mousemode right "translate selected models" |
| 15393 | | |
| 15394 | | > ui mousemode right translate |
| 15395 | | |
| 15396 | | > view P1 |
| 15397 | | |
| 15398 | | > save CS_P4_J255_NU_sharp.png supersample 3 transparentBackground true |
| 15399 | | |
| 15400 | | > hide #!25 models |
| 15401 | | |
| 15402 | | > show #!26 models |
| 15403 | | |
| 15404 | | > volume #26 level 1.012 |
| 15405 | | |
| 15406 | | > save CS_P4_J256_NU_sharp.png supersample 3 transparentBackground true |
| 15407 | | |
| 15408 | | > hide #!26 models |
| 15409 | | |
| 15410 | | > show #!27 models |
| 15411 | | |
| 15412 | | > save CS_P4_J257_NU_sharp.png supersample 3 transparentBackground true |
| 15413 | | |
| 15414 | | > hide #!27 models |
| 15415 | | |
| 15416 | | > show #!28 models |
| 15417 | | |
| 15418 | | > save CS_P4_J258_NU_sharp.png supersample 3 transparentBackground true |
| 15419 | | |
| 15420 | | > hide #!28 models |
| 15421 | | |
| 15422 | | > show #!29 models |
| 15423 | | |
| 15424 | | > save CS_P4_J259_NU_sharp.png supersample 3 transparentBackground true |
| 15425 | | |
| 15426 | | > save "/Users/peiyuyu/Documents/Goethe |
| 15427 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 15428 | | > F_B1/20260502_SA-F_B1_workflow.cxs" |
| 15429 | | |
| 15430 | | > open "/Users/peiyuyu/Documents/Goethe |
| 15431 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 15432 | | > F_B1/cryosparc_P4_J285_006_volume_map_sharp.mrc" |
| 15433 | | |
| 15434 | | Opened cryosparc_P4_J285_006_volume_map_sharp.mrc as #30, grid size |
| 15435 | | 320,320,320, pixel 0.831, shown at level 0.0363, step 2, values float32 |
| 15436 | | |
| 15437 | | > open "/Users/peiyuyu/Documents/Goethe |
| 15438 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 15439 | | > F_B1/cryosparc_P4_J267_008_volume_map_sharp.mrc" |
| 15440 | | |
| 15441 | | Opened cryosparc_P4_J267_008_volume_map_sharp.mrc as #31, grid size |
| 15442 | | 320,320,320, pixel 0.831, shown at level 0.0747, step 2, values float32 |
| 15443 | | |
| 15444 | | > open "/Users/peiyuyu/Documents/Goethe |
| 15445 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 15446 | | > F_B1/cryosparc_P4_J277_008_volume_map_sharp.mrc" |
| 15447 | | |
| 15448 | | Opened cryosparc_P4_J277_008_volume_map_sharp.mrc as #32, grid size |
| 15449 | | 320,320,320, pixel 0.831, shown at level 0.0762, step 2, values float32 |
| 15450 | | |
| 15451 | | > hide #!29 models |
| 15452 | | |
| 15453 | | > hide #!30 models |
| 15454 | | |
| 15455 | | > hide #!31 models |
| 15456 | | |
| 15457 | | > show #!31 models |
| 15458 | | |
| 15459 | | > hide #!32 models |
| 15460 | | |
| 15461 | | > show #!30 models |
| 15462 | | |
| 15463 | | > close #30 |
| 15464 | | |
| 15465 | | > show #!27 models |
| 15466 | | |
| 15467 | | > select add #31 |
| 15468 | | |
| 15469 | | 2 models selected |
| 15470 | | |
| 15471 | | > ui mousemode right "translate selected models" |
| 15472 | | |
| 15473 | | > ui mousemode right "rotate selected models" |
| 15474 | | |
| 15475 | | > view matrix models |
| 15476 | | > #31,-0.90396,0.26529,0.33537,196.37,0.29187,0.95597,0.030503,-41.724,-0.31251,0.12546,-0.94159,315.51 |
| 15477 | | |
| 15478 | | > hide #!27 models |
| 15479 | | |
| 15480 | | > view matrix models |
| 15481 | | > #31,-0.95736,-0.095902,0.27253,266.49,-0.06692,0.99125,0.11374,-5.5307,-0.28105,0.090649,-0.9554,317.94 |
| 15482 | | |
| 15483 | | > show #!29 models |
| 15484 | | |
| 15485 | | > hide #!29 models |
| 15486 | | |
| 15487 | | > select subtract #31 |
| 15488 | | |
| 15489 | | Nothing selected |
| 15490 | | |
| 15491 | | > close #32 |
| 15492 | | |
| 15493 | | > volume flip #31 |
| 15494 | | |
| 15495 | | Opened cryosparc_P4_J267_008_volume_map_sharp.mrc z flip as #30, grid size |
| 15496 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 15497 | | |
| 15498 | | > close #30 |
| 15499 | | |
| 15500 | | > show #!31 models |
| 15501 | | |
| 15502 | | > select add #31 |
| 15503 | | |
| 15504 | | 2 models selected |
| 15505 | | |
| 15506 | | > show #!27 models |
| 15507 | | |
| 15508 | | > ui mousemode right "translate selected models" |
| 15509 | | |
| 15510 | | > view matrix models |
| 15511 | | > #31,-0.95736,-0.095902,0.27253,258.1,-0.06692,0.99125,0.11374,14.401,-0.28105,0.090649,-0.9554,295.97 |
| 15512 | | |
| 15513 | | > ui mousemode right "rotate selected models" |
| 15514 | | |
| 15515 | | > view matrix models |
| 15516 | | > #31,0.57983,0.79903,0.15922,-85.975,0.81408,-0.57604,-0.073785,139.64,0.032763,0.1724,-0.98448,241.38 |
| 15517 | | |
| 15518 | | > ui mousemode right "translate selected models" |
| 15519 | | |
| 15520 | | > view matrix models |
| 15521 | | > #31,0.57983,0.79903,0.15922,-74.26,0.81408,-0.57604,-0.073785,114.14,0.032763,0.1724,-0.98448,243.66 |
| 15522 | | |
| 15523 | | > ui mousemode right "rotate selected models" |
| 15524 | | |
| 15525 | | > view matrix models |
| 15526 | | > #31,0.38773,0.91358,0.12259,-56.869,0.92165,-0.3821,-0.067497,68.753,-0.014823,0.13915,-0.99016,256.48 |
| 15527 | | |
| 15528 | | > ui mousemode right "translate selected models" |
| 15529 | | |
| 15530 | | > view matrix models |
| 15531 | | > #31,0.38773,0.91358,0.12259,-59.17,0.92165,-0.3821,-0.067497,73.776,-0.014823,0.13915,-0.99016,255.81 |
| 15532 | | |
| 15533 | | > fitmap #31 inMap #27 |
| 15534 | | |
| 15535 | | Fit map cryosparc_P4_J267_008_volume_map_sharp.mrc in map |
| 15536 | | CS_P4_J257_sharp_flip.mrc using 40948 points |
| 15537 | | correlation = 0.4368, correlation about mean = 0.04808, overlap = 2838 |
| 15538 | | steps = 84, shift = 6.03, angle = 16 degrees |
| 15539 | | |
| 15540 | | Position of cryosparc_P4_J267_008_volume_map_sharp.mrc (#31) relative to |
| 15541 | | CS_P4_J257_sharp_flip.mrc (#27) coordinates: |
| 15542 | | Matrix rotation and translation |
| 15543 | | -0.09805932 0.98945486 0.10659935 2.84911711 |
| 15544 | | 0.99008140 0.10782511 -0.09006967 -2.59568012 |
| 15545 | | -0.10061396 0.09670986 -0.99021412 262.50076525 |
| 15546 | | Axis 0.66953234 0.74277951 0.00224590 |
| 15547 | | Axis point 14.97851108 -0.00000000 131.13786872 |
| 15548 | | Rotation angle (degrees) 171.98194915 |
| 15549 | | Shift along axis 0.56910865 |
| 15550 | | |
| 15551 | | |
| 15552 | | > select subtract #31 |
| 15553 | | |
| 15554 | | Nothing selected |
| 15555 | | |
| 15556 | | > hide #!31 models |
| 15557 | | |
| 15558 | | > hide #!27 models |
| 15559 | | |
| 15560 | | > show #!31 models |
| 15561 | | |
| 15562 | | > close #31 |
| 15563 | | |
| 15564 | | > open "/Users/peiyuyu/Documents/Goethe |
| 15565 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 15566 | | > F_B1/cryosparc_P4_J267_008_volume_map_sharp.mrc" |
| 15567 | | |
| 15568 | | Opened cryosparc_P4_J267_008_volume_map_sharp.mrc as #30, grid size |
| 15569 | | 320,320,320, pixel 0.831, shown at level 0.0747, step 2, values float32 |
| 15570 | | |
| 15571 | | > select add #30 |
| 15572 | | |
| 15573 | | 2 models selected |
| 15574 | | |
| 15575 | | > show #!27 models |
| 15576 | | |
| 15577 | | > ui mousemode right "rotate selected models" |
| 15578 | | |
| 15579 | | > view matrix models |
| 15580 | | > #30,0.59059,-0.80637,-0.031153,183.8,-0.80688,-0.59066,-0.008015,354.56,-0.011937,0.02987,-0.99948,293.1 |
| 15581 | | |
| 15582 | | > ui mousemode right "translate selected models" |
| 15583 | | |
| 15584 | | > view matrix models |
| 15585 | | > #30,0.59059,-0.80637,-0.031153,187.84,-0.80688,-0.59066,-0.008015,342.67,-0.011937,0.02987,-0.99948,267.57 |
| 15586 | | |
| 15587 | | > hide #!27 models |
| 15588 | | |
| 15589 | | > show #!27 models |
| 15590 | | |
| 15591 | | > hide #!30 models |
| 15592 | | |
| 15593 | | > hide #!27 models |
| 15594 | | |
| 15595 | | > show #!30 models |
| 15596 | | |
| 15597 | | > volume flip #30 |
| 15598 | | |
| 15599 | | Opened cryosparc_P4_J267_008_volume_map_sharp.mrc z flip as #31, grid size |
| 15600 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 15601 | | |
| 15602 | | > select subtract #30 |
| 15603 | | |
| 15604 | | Nothing selected |
| 15605 | | |
| 15606 | | > close #30 |
| 15607 | | |
| 15608 | | > select add #31 |
| 15609 | | |
| 15610 | | 2 models selected |
| 15611 | | |
| 15612 | | > show #!27 models |
| 15613 | | |
| 15614 | | > view matrix models |
| 15615 | | > #31,0.59059,-0.80637,-0.031153,181.08,-0.80688,-0.59066,-0.008015,342.68,-0.011937,0.02987,-0.99948,285.6 |
| 15616 | | |
| 15617 | | > ui mousemode right "rotate selected models" |
| 15618 | | |
| 15619 | | > view matrix models |
| 15620 | | > #31,0.49825,-0.85813,0.12394,182.63,-0.032454,0.12438,0.9917,19.314,-0.86642,-0.49814,0.034125,354.11 |
| 15621 | | |
| 15622 | | > view matrix models |
| 15623 | | > #31,-0.47795,-0.87619,-0.061991,343.39,0.85267,-0.47976,0.20686,70.492,-0.21099,0.046011,0.9764,77.316 |
| 15624 | | |
| 15625 | | > ui mousemode right "translate selected models" |
| 15626 | | |
| 15627 | | > view matrix models |
| 15628 | | > #31,-0.47795,-0.87619,-0.061991,322.39,0.85267,-0.47976,0.20686,71.691,-0.21099,0.046011,0.9764,36.515 |
| 15629 | | |
| 15630 | | > view matrix models |
| 15631 | | > #31,-0.47795,-0.87619,-0.061991,329.78,0.85267,-0.47976,0.20686,58.248,-0.21099,0.046011,0.9764,36.244 |
| 15632 | | |
| 15633 | | > fitmap #31 inMap #27 |
| 15634 | | |
| 15635 | | Fit map cryosparc_P4_J267_008_volume_map_sharp.mrc z flip in map |
| 15636 | | CS_P4_J257_sharp_flip.mrc using 327854 points |
| 15637 | | correlation = 0.6967, correlation about mean = 0.2684, overlap = 4.49e+04 |
| 15638 | | steps = 92, shift = 8.63, angle = 16.2 degrees |
| 15639 | | |
| 15640 | | Position of cryosparc_P4_J267_008_volume_map_sharp.mrc z flip (#31) relative |
| 15641 | | to CS_P4_J257_sharp_flip.mrc (#27) coordinates: |
| 15642 | | Matrix rotation and translation |
| 15643 | | -0.48810574 -0.85845883 -0.15748400 338.09346066 |
| 15644 | | 0.86132304 -0.50293557 0.07196129 74.97027819 |
| 15645 | | -0.14098011 -0.10051988 0.98489611 32.48936744 |
| 15646 | | Axis -0.09978729 -0.00954816 0.99496298 |
| 15647 | | Axis point 149.01931346 135.27108975 0.00000000 |
| 15648 | | Rotation angle (degrees) 120.20349080 |
| 15649 | | Shift along axis -2.12754240 |
| 15650 | | |
| 15651 | | |
| 15652 | | > select subtract #31 |
| 15653 | | |
| 15654 | | Nothing selected |
| 15655 | | |
| 15656 | | > volume #27 level 1.164 |
| 15657 | | |
| 15658 | | > volume #31 level 0.1055 |
| 15659 | | |
| 15660 | | > hide #!27 models |
| 15661 | | |
| 15662 | | > volume #31 level 0.1343 |
| 15663 | | |
| 15664 | | > color #31 #99BFE5 |
| 15665 | | |
| 15666 | | > rename #31 CS_P4_J267_sharp_flip id #30 |
| 15667 | | |
| 15668 | | > save CS_P4_J267_sharp_flip.mrc #30 |
| 15669 | | |
| 15670 | | > open "/Users/peiyuyu/Documents/Goethe |
| 15671 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 15672 | | > F_B1/cryosparc_P4_J277_008_volume_map_sharp.mrc" |
| 15673 | | |
| 15674 | | Opened cryosparc_P4_J277_008_volume_map_sharp.mrc as #31, grid size |
| 15675 | | 320,320,320, pixel 0.831, shown at level 0.0762, step 2, values float32 |
| 15676 | | |
| 15677 | | > hide #!30 models |
| 15678 | | |
| 15679 | | > select add #31 |
| 15680 | | |
| 15681 | | 2 models selected |
| 15682 | | |
| 15683 | | > ui mousemode right "rotate selected models" |
| 15684 | | |
| 15685 | | > view matrix models |
| 15686 | | > #31,0.50169,-0.77701,0.38022,112.38,-0.8444,-0.53533,0.020189,301.01,0.18786,-0.33119,-0.92467,273.22 |
| 15687 | | |
| 15688 | | > view matrix models |
| 15689 | | > #31,0.77218,-0.62718,0.10187,94.664,-0.63536,-0.764,0.11236,288.83,0.0073633,-0.15149,-0.98843,283.82 |
| 15690 | | |
| 15691 | | > volume flip #31 |
| 15692 | | |
| 15693 | | Opened cryosparc_P4_J277_008_volume_map_sharp.mrc z flip as #32, grid size |
| 15694 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 15695 | | |
| 15696 | | > close #31 |
| 15697 | | |
| 15698 | | > select add #32 |
| 15699 | | |
| 15700 | | 2 models selected |
| 15701 | | |
| 15702 | | > show #!30 models |
| 15703 | | |
| 15704 | | > view matrix models |
| 15705 | | > #32,0.90608,0.27127,-0.3247,15.596,-0.28323,0.95899,0.010829,28.44,0.31432,0.082155,0.94575,-47.16 |
| 15706 | | |
| 15707 | | > view matrix models |
| 15708 | | > #32,0.99239,-0.018676,-0.12173,15.559,0.019459,0.9998,0.0052468,-15.401,0.1216,-0.0075755,0.99255,-16.612 |
| 15709 | | |
| 15710 | | > ui mousemode right "translate selected models" |
| 15711 | | |
| 15712 | | > view matrix models |
| 15713 | | > #32,0.99239,-0.018676,-0.12173,21.14,0.019459,0.9998,0.0052468,-2.6957,0.1216,-0.0075755,0.99255,-9.4459 |
| 15714 | | |
| 15715 | | > fitmap #32 inMap #30 |
| 15716 | | |
| 15717 | | Fit map cryosparc_P4_J277_008_volume_map_sharp.mrc z flip in map |
| 15718 | | CS_P4_J267_sharp_flip.mrc using 327711 points |
| 15719 | | correlation = 0.7196, correlation about mean = 0.3385, overlap = 5671 |
| 15720 | | steps = 204, shift = 3.68, angle = 13.7 degrees |
| 15721 | | |
| 15722 | | Position of cryosparc_P4_J277_008_volume_map_sharp.mrc z flip (#32) relative |
| 15723 | | to CS_P4_J267_sharp_flip.mrc (#30) coordinates: |
| 15724 | | Matrix rotation and translation |
| 15725 | | -0.48144357 0.86501463 -0.14128627 103.86408789 |
| 15726 | | -0.86432037 -0.49531179 -0.08727288 328.52215562 |
| 15727 | | -0.14547307 0.08009963 0.98611442 10.75546195 |
| 15728 | | Axis 0.09633390 0.00240978 -0.99534616 |
| 15729 | | Axis point 148.32654443 133.96556119 0.00000000 |
| 15730 | | Rotation angle (degrees) 119.69088439 |
| 15731 | | Shift along axis 0.09189113 |
| 15732 | | |
| 15733 | | |
| 15734 | | > select subtract #32 |
| 15735 | | |
| 15736 | | Nothing selected |
| 15737 | | |
| 15738 | | > volume #32 level 0.1306 |
| 15739 | | |
| 15740 | | > hide #!30 models |
| 15741 | | |
| 15742 | | > volume #32 level 0.1432 |
| 15743 | | |
| 15744 | | > color #32 #99BFE5 |
| 15745 | | |
| 15746 | | > rename #32 CS_P4_J277_sharp_flip id #31 |
| 15747 | | |
| 15748 | | > save CS_P4_J277_sharp_flip.mrc #31 |
| 15749 | | |
| 15750 | | > open "/Users/peiyuyu/Documents/Goethe |
| 15751 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 15752 | | > F_B1/cryosparc_P4_J285_006_volume_map_sharp.mrc" |
| 15753 | | |
| 15754 | | Opened cryosparc_P4_J285_006_volume_map_sharp.mrc as #32, grid size |
| 15755 | | 320,320,320, pixel 0.831, shown at level 0.0363, step 2, values float32 |
| 15756 | | |
| 15757 | | > hide #!31 models |
| 15758 | | |
| 15759 | | > select add #32 |
| 15760 | | |
| 15761 | | 2 models selected |
| 15762 | | |
| 15763 | | > ui mousemode right "rotate selected models" |
| 15764 | | |
| 15765 | | > view matrix models |
| 15766 | | > #32,0.30706,0.95043,0.049005,-33.318,0.9497,-0.30268,-0.080397,47.411,-0.061579,0.071226,-0.99556,261.24 |
| 15767 | | |
| 15768 | | > volume flip #32 |
| 15769 | | |
| 15770 | | Opened cryosparc_P4_J285_006_volume_map_sharp.mrc z flip as #33, grid size |
| 15771 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 15772 | | |
| 15773 | | > show #!31 models |
| 15774 | | |
| 15775 | | > show #!32 models |
| 15776 | | |
| 15777 | | > view matrix models |
| 15778 | | > #32,0.19586,0.82677,-0.52734,72.951,0.95192,-0.28947,-0.10029,48.053,-0.23556,-0.48235,-0.84371,334.74 |
| 15779 | | |
| 15780 | | > view matrix models |
| 15781 | | > #32,0.038463,0.98737,0.15371,-15.568,0.97818,-0.0057722,-0.20769,22.74,-0.20418,0.15835,-0.96604,265.55 |
| 15782 | | |
| 15783 | | > close #32 |
| 15784 | | |
| 15785 | | > select add #33 |
| 15786 | | |
| 15787 | | 2 models selected |
| 15788 | | |
| 15789 | | > view matrix models |
| 15790 | | > #33,-0.36526,-0.91739,-0.15805,324.89,0.9276,-0.34439,-0.14472,64.223,0.078335,-0.19947,0.97677,17.619 |
| 15791 | | |
| 15792 | | > view matrix models |
| 15793 | | > #33,-0.3296,-0.92737,-0.17708,323.89,0.91639,-0.35937,0.17633,25.386,-0.22716,-0.10416,0.96827,47.503 |
| 15794 | | |
| 15795 | | > select subtract #33 |
| 15796 | | |
| 15797 | | Nothing selected |
| 15798 | | |
| 15799 | | > fitmap #33 inMap #31 |
| 15800 | | |
| 15801 | | Fit map cryosparc_P4_J285_006_volume_map_sharp.mrc z flip in map |
| 15802 | | CS_P4_J277_sharp_flip.mrc using 327344 points |
| 15803 | | correlation = 0.3754, correlation about mean = 0.04431, overlap = 1099 |
| 15804 | | steps = 64, shift = 4.4, angle = 1.24 degrees |
| 15805 | | |
| 15806 | | Position of cryosparc_P4_J285_006_volume_map_sharp.mrc z flip (#33) relative |
| 15807 | | to CS_P4_J277_sharp_flip.mrc (#31) coordinates: |
| 15808 | | Matrix rotation and translation |
| 15809 | | -0.52307807 -0.83656982 -0.16291178 338.77635518 |
| 15810 | | 0.81824450 -0.54640858 0.17864376 66.60900444 |
| 15811 | | -0.23846438 -0.03985703 0.97033300 35.38091339 |
| 15812 | | Axis -0.13076939 0.04521708 0.99038113 |
| 15813 | | Axis point 153.51682890 124.48577590 0.00000000 |
| 15814 | | Rotation angle (degrees) 123.33798629 |
| 15815 | | Shift along axis -6.24912375 |
| 15816 | | |
| 15817 | | |
| 15818 | | > volume #33 level 0.05666 |
| 15819 | | |
| 15820 | | > select add #33 |
| 15821 | | |
| 15822 | | 2 models selected |
| 15823 | | |
| 15824 | | > ui mousemode right "translate selected models" |
| 15825 | | |
| 15826 | | > view matrix models |
| 15827 | | > #33,-0.32295,-0.92807,-0.18543,326.53,0.9135,-0.3569,0.19529,26.322,-0.24742,-0.10632,0.96306,60.406 |
| 15828 | | |
| 15829 | | > fitmap #33 inMap #31 |
| 15830 | | |
| 15831 | | Fit map cryosparc_P4_J285_006_volume_map_sharp.mrc z flip in map |
| 15832 | | CS_P4_J277_sharp_flip.mrc using 194771 points |
| 15833 | | correlation = 0.7316, correlation about mean = 0.2783, overlap = 2429 |
| 15834 | | steps = 104, shift = 3.72, angle = 6.69 degrees |
| 15835 | | |
| 15836 | | Position of cryosparc_P4_J285_006_volume_map_sharp.mrc z flip (#33) relative |
| 15837 | | to CS_P4_J277_sharp_flip.mrc (#31) coordinates: |
| 15838 | | Matrix rotation and translation |
| 15839 | | -0.48329817 -0.86312458 -0.14641999 335.54076959 |
| 15840 | | 0.86340511 -0.49758655 0.08330200 72.08568886 |
| 15841 | | -0.14475663 -0.08616006 0.98570885 33.43360614 |
| 15842 | | Axis -0.09768239 -0.00095880 0.99521718 |
| 15843 | | Axis point 148.43445219 133.71191357 0.00000000 |
| 15844 | | Rotation angle (degrees) 119.84054702 |
| 15845 | | Shift along axis 0.42815717 |
| 15846 | | |
| 15847 | | |
| 15848 | | > select subtract #33 |
| 15849 | | |
| 15850 | | Nothing selected |
| 15851 | | |
| 15852 | | > select add #33 |
| 15853 | | |
| 15854 | | 2 models selected |
| 15855 | | |
| 15856 | | > ui mousemode right "rotate selected models" |
| 15857 | | |
| 15858 | | > view matrix models |
| 15859 | | > #33,-0.6364,0.75107,-0.17577,140.89,-0.74512,-0.6575,-0.11171,345.32,-0.19948,0.059876,0.97807,29.849 |
| 15860 | | |
| 15861 | | > ui mousemode right "translate selected models" |
| 15862 | | |
| 15863 | | > view matrix models |
| 15864 | | > #33,-0.6364,0.75107,-0.17577,136.82,-0.74512,-0.6575,-0.11171,338.86,-0.19948,0.059876,0.97807,29.687 |
| 15865 | | |
| 15866 | | > ui mousemode right "rotate selected models" |
| 15867 | | |
| 15868 | | > view matrix models |
| 15869 | | > #33,-0.73358,0.64738,-0.20678,168.84,-0.63708,-0.76101,-0.12246,339.46,-0.23664,0.041901,0.97069,38.344 |
| 15870 | | |
| 15871 | | > fitmap #33 inMap #31 |
| 15872 | | |
| 15873 | | Fit map cryosparc_P4_J285_006_volume_map_sharp.mrc z flip in map |
| 15874 | | CS_P4_J277_sharp_flip.mrc using 194771 points |
| 15875 | | correlation = 0.4346, correlation about mean = 0.05784, overlap = 1046 |
| 15876 | | steps = 96, shift = 4.05, angle = 4.16 degrees |
| 15877 | | |
| 15878 | | Position of cryosparc_P4_J285_006_volume_map_sharp.mrc z flip (#33) relative |
| 15879 | | to CS_P4_J277_sharp_flip.mrc (#31) coordinates: |
| 15880 | | Matrix rotation and translation |
| 15881 | | -0.58502424 0.80932802 -0.05229508 107.72395607 |
| 15882 | | -0.80221021 -0.58694418 -0.10934029 332.49911712 |
| 15883 | | -0.11918646 -0.02201508 0.99262777 22.68232922 |
| 15884 | | Axis 0.05406170 0.04141143 -0.99767852 |
| 15885 | | Axis point 139.16943260 139.24984383 0.00000000 |
| 15886 | | Rotation angle (degrees) 126.13361686 |
| 15887 | | Shift along axis -3.03667024 |
| 15888 | | |
| 15889 | | |
| 15890 | | > ui mousemode right "translate selected models" |
| 15891 | | |
| 15892 | | > view matrix models |
| 15893 | | > #33,-0.7311,0.66788,-0.13939,159.58,-0.65889,-0.74418,-0.10984,339.55,-0.17709,0.011538,0.98413,33.11 |
| 15894 | | |
| 15895 | | > fitmap #33 inMap #31 |
| 15896 | | |
| 15897 | | Fit map cryosparc_P4_J285_006_volume_map_sharp.mrc z flip in map |
| 15898 | | CS_P4_J277_sharp_flip.mrc using 194771 points |
| 15899 | | correlation = 0.7261, correlation about mean = 0.2429, overlap = 2435 |
| 15900 | | steps = 148, shift = 3.57, angle = 8.91 degrees |
| 15901 | | |
| 15902 | | Position of cryosparc_P4_J285_006_volume_map_sharp.mrc z flip (#33) relative |
| 15903 | | to CS_P4_J277_sharp_flip.mrc (#31) coordinates: |
| 15904 | | Matrix rotation and translation |
| 15905 | | -0.47691102 0.86730315 -0.14262219 103.16958211 |
| 15906 | | -0.86637561 -0.49120809 -0.09004393 328.47939238 |
| 15907 | | -0.14815256 0.08062145 0.98567285 11.48812128 |
| 15908 | | Axis 0.09796714 0.00317460 -0.99518459 |
| 15909 | | Axis point 148.54824739 133.97305983 0.00000000 |
| 15910 | | Rotation angle (degrees) 119.42100612 |
| 15911 | | Shift along axis -0.28278057 |
| 15912 | | |
| 15913 | | |
| 15914 | | > ui mousemode right "rotate selected models" |
| 15915 | | |
| 15916 | | > view matrix models |
| 15917 | | > #33,0.98687,0.16137,0.0076046,-28.356,-0.15803,0.97406,-0.16197,41.243,-0.033544,0.15864,0.98677,-7.5792 |
| 15918 | | |
| 15919 | | > ui mousemode right "translate selected models" |
| 15920 | | |
| 15921 | | > view matrix models |
| 15922 | | > #33,0.98687,0.16137,0.0076046,-22.398,-0.15803,0.97406,-0.16197,51.235,-0.033544,0.15864,0.98677,-4.7399 |
| 15923 | | |
| 15924 | | > fitmap #33 inMap #31 |
| 15925 | | |
| 15926 | | Fit map cryosparc_P4_J285_006_volume_map_sharp.mrc z flip in map |
| 15927 | | CS_P4_J277_sharp_flip.mrc using 194771 points |
| 15928 | | correlation = 0.9896, correlation about mean = 0.9159, overlap = 4214 |
| 15929 | | steps = 120, shift = 4.17, angle = 9.65 degrees |
| 15930 | | |
| 15931 | | Position of cryosparc_P4_J285_006_volume_map_sharp.mrc z flip (#33) relative |
| 15932 | | to CS_P4_J277_sharp_flip.mrc (#31) coordinates: |
| 15933 | | Matrix rotation and translation |
| 15934 | | 0.99997973 -0.00439362 0.00460748 -0.02530815 |
| 15935 | | 0.00439161 0.99999024 0.00044636 -0.51759472 |
| 15936 | | -0.00460939 -0.00042611 0.99998927 1.01725436 |
| 15937 | | Axis -0.06835965 0.72216012 0.68833983 |
| 15938 | | Axis point 171.68283436 0.00000000 1.37920014 |
| 15939 | | Rotation angle (degrees) 0.36563312 |
| 15940 | | Shift along axis 0.32816049 |
| 15941 | | |
| 15942 | | |
| 15943 | | > select subtract #33 |
| 15944 | | |
| 15945 | | Nothing selected |
| 15946 | | |
| 15947 | | > hide #!31 models |
| 15948 | | |
| 15949 | | > show #!31 models |
| 15950 | | |
| 15951 | | > hide #!31 models |
| 15952 | | |
| 15953 | | > volume #33 level 0.06481 |
| 15954 | | |
| 15955 | | > color #33 #99BFE5 |
| 15956 | | |
| 15957 | | > view P1 |
| 15958 | | |
| 15959 | | > rename #33 CS_P4_J285_sharp_flip id #32 |
| 15960 | | |
| 15961 | | > save CS_P4_J285_sharp_flip.mrc #32 |
| 15962 | | |
| 15963 | | > save "/Users/peiyuyu/Documents/Goethe |
| 15964 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 15965 | | > F_B1/20260502_SA-F_B1_workflow.cxs" |
| 15966 | | |
| 15967 | | > save CS_P4_J285_NU_sharp.png supersample 3 transparentBackground true |
| 15968 | | |
| 15969 | | > hide #!32 models |
| 15970 | | |
| 15971 | | > show #!31 models |
| 15972 | | |
| 15973 | | > save CS_P4_J277_NU_sharp.png supersample 3 transparentBackground true |
| 15974 | | |
| 15975 | | > hide #!31 models |
| 15976 | | |
| 15977 | | > show #!30 models |
| 15978 | | |
| 15979 | | > save CS_P4_J267_NU_sharp.png supersample 3 transparentBackground true |
| 15980 | | |
| 15981 | | > open "/Users/peiyuyu/Documents/Goethe |
| 15982 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 15983 | | > F_B1/cryosparc_P4_J282_005_volume_map_sharp.mrc" |
| 15984 | | |
| 15985 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc as #33, grid size |
| 15986 | | 320,320,320, pixel 0.831, shown at level 0.0392, step 2, values float32 |
| 15987 | | |
| 15988 | | > select add #33 |
| 15989 | | |
| 15990 | | 2 models selected |
| 15991 | | |
| 15992 | | > ui mousemode right "rotate selected models" |
| 15993 | | |
| 15994 | | > view matrix models |
| 15995 | | > #33,0.77382,-0.58736,0.23709,68.017,-0.6199,-0.77915,0.092993,287.71,0.13011,-0.21893,-0.96703,293.89 |
| 15996 | | |
| 15997 | | > volume flip sel |
| 15998 | | |
| 15999 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc z flip as #34, grid size |
| 16000 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 16001 | | |
| 16002 | | > select subtract #33 |
| 16003 | | |
| 16004 | | Nothing selected |
| 16005 | | |
| 16006 | | > select add #34 |
| 16007 | | |
| 16008 | | 2 models selected |
| 16009 | | |
| 16010 | | > view matrix models |
| 16011 | | > #34,0.96024,0.18105,-0.2125,4.9844,-0.18441,0.98284,0.0040821,22.146,0.20959,0.035266,0.97715,4.1522 |
| 16012 | | |
| 16013 | | > volume #34 level 0.06153 |
| 16014 | | |
| 16015 | | > ui mousemode right "translate selected models" |
| 16016 | | |
| 16017 | | > view matrix models |
| 16018 | | > #34,0.96024,0.18105,-0.2125,5.3992,-0.18441,0.98284,0.0040821,24.944,0.20959,0.035266,0.97715,-19.851 |
| 16019 | | |
| 16020 | | > fitmap #34 inMap #30 |
| 16021 | | |
| 16022 | | Fit map cryosparc_P4_J282_005_volume_map_sharp.mrc z flip in map |
| 16023 | | CS_P4_J267_sharp_flip.mrc using 179913 points |
| 16024 | | correlation = 0.7668, correlation about mean = 0.2691, overlap = 2384 |
| 16025 | | steps = 132, shift = 4.76, angle = 10.2 degrees |
| 16026 | | |
| 16027 | | Position of cryosparc_P4_J282_005_volume_map_sharp.mrc z flip (#34) relative |
| 16028 | | to CS_P4_J267_sharp_flip.mrc (#30) coordinates: |
| 16029 | | Matrix rotation and translation |
| 16030 | | -0.47859383 0.86460690 -0.15297990 105.65226772 |
| 16031 | | -0.86128787 -0.49615211 -0.10961884 331.90892973 |
| 16032 | | -0.17067851 0.07929683 0.98213077 14.98614385 |
| 16033 | | Axis 0.10880400 0.01019333 -0.99401096 |
| 16034 | | Axis point 150.51207819 135.19673705 0.00000000 |
| 16035 | | Rotation angle (degrees) 119.75601097 |
| 16036 | | Shift along axis -0.01774616 |
| 16037 | | |
| 16038 | | |
| 16039 | | > select subtract #34 |
| 16040 | | |
| 16041 | | Nothing selected |
| 16042 | | |
| 16043 | | > select add #34 |
| 16044 | | |
| 16045 | | 2 models selected |
| 16046 | | |
| 16047 | | > ui mousemode right "rotate selected models" |
| 16048 | | |
| 16049 | | > view matrix models |
| 16050 | | > #34,-0.29858,-0.92833,-0.22151,340.08,0.93935,-0.32688,0.10377,36.917,-0.16874,-0.17709,0.96962,60.484 |
| 16051 | | |
| 16052 | | > fitmap #34 inMap #30 |
| 16053 | | |
| 16054 | | Fit map cryosparc_P4_J282_005_volume_map_sharp.mrc z flip in map |
| 16055 | | CS_P4_J267_sharp_flip.mrc using 179913 points |
| 16056 | | correlation = 0.4184, correlation about mean = 0.03499, overlap = 815.8 |
| 16057 | | steps = 72, shift = 3.19, angle = 2.56 degrees |
| 16058 | | |
| 16059 | | Position of cryosparc_P4_J282_005_volume_map_sharp.mrc z flip (#34) relative |
| 16060 | | to CS_P4_J267_sharp_flip.mrc (#30) coordinates: |
| 16061 | | Matrix rotation and translation |
| 16062 | | 0.99975679 -0.00914856 -0.02006678 -0.29483780 |
| 16063 | | 0.00937915 0.99989072 0.01142689 -12.23865962 |
| 16064 | | 0.01996005 -0.01161232 0.99973334 -0.12571505 |
| 16065 | | Axis -0.46299067 -0.80436995 0.37232865 |
| 16066 | | Axis point 194.44052306 0.00000000 212.15518455 |
| 16067 | | Rotation angle (degrees) 1.42571524 |
| 16068 | | Shift along axis 9.93410983 |
| 16069 | | |
| 16070 | | |
| 16071 | | > ui mousemode right "translate selected models" |
| 16072 | | |
| 16073 | | > view matrix models |
| 16074 | | > #34,-0.29149,-0.93644,-0.19523,323.34,0.9479,-0.31019,0.07257,35.755,-0.12852,-0.16391,0.97807,48.671 |
| 16075 | | |
| 16076 | | > fitmap #34 inMap #30 |
| 16077 | | |
| 16078 | | Fit map cryosparc_P4_J282_005_volume_map_sharp.mrc z flip in map |
| 16079 | | CS_P4_J267_sharp_flip.mrc using 179913 points |
| 16080 | | correlation = 0.9696, correlation about mean = 0.7683, overlap = 3705 |
| 16081 | | steps = 76, shift = 2.39, angle = 2.68 degrees |
| 16082 | | |
| 16083 | | Position of cryosparc_P4_J282_005_volume_map_sharp.mrc z flip (#34) relative |
| 16084 | | to CS_P4_J267_sharp_flip.mrc (#30) coordinates: |
| 16085 | | Matrix rotation and translation |
| 16086 | | 0.99970034 -0.00006024 0.02447927 -4.11138943 |
| 16087 | | 0.00004629 0.99999984 0.00057062 0.05899118 |
| 16088 | | -0.02447930 -0.00056932 0.99970018 3.40337319 |
| 16089 | | Axis -0.02327752 0.99972667 0.00217546 |
| 16090 | | Axis point 136.94050248 0.00000000 169.47617625 |
| 16091 | | Rotation angle (degrees) 1.40308329 |
| 16092 | | Shift along axis 0.16208189 |
| 16093 | | |
| 16094 | | |
| 16095 | | > select subtract #34 |
| 16096 | | |
| 16097 | | Nothing selected |
| 16098 | | |
| 16099 | | > hide #!30 models |
| 16100 | | |
| 16101 | | > show #!32 models |
| 16102 | | |
| 16103 | | > hide #!31 models |
| 16104 | | |
| 16105 | | > volume #34 level 0.06805 |
| 16106 | | |
| 16107 | | > hide #!32 models |
| 16108 | | |
| 16109 | | > view P1 |
| 16110 | | |
| 16111 | | > close #33 |
| 16112 | | |
| 16113 | | > rename #34 CS_P4_J282_sharp_flip id #33 |
| 16114 | | |
| 16115 | | > save CS_P4_J282_sharp_flip.mrc #33 |
| 16116 | | |
| 16117 | | > view P1 |
| 16118 | | |
| 16119 | | > save CS_P4_J282_NU_sharp.png supersample 3 transparentBackground true |
| 16120 | | |
| 16121 | | > save "/Users/peiyuyu/Documents/Goethe |
| 16122 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 16123 | | > F_B1/20260502_SA-F_B1_workflow1.cxs" |
| 16124 | | |
| 16125 | | > color #33 #99BFE5 |
| 16126 | | |
| 16127 | | > save CS_P4_J282_NU_sharp1.png supersample 3 transparentBackground true |
| 16128 | | |
| 16129 | | > save "/Users/peiyuyu/Documents/Goethe |
| 16130 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 16131 | | > F_B1/20260502_SA-F_B1_workflow1.cxs" |
| 16132 | | |
| 16133 | | > open "/Users/peiyuyu/Documents/Goethe |
| 16134 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 16135 | | > F_B1/cryosparc_P4_J287_map_locres.mrc" |
| 16136 | | |
| 16137 | | Opened cryosparc_P4_J287_map_locres.mrc as #34, grid size 320,320,320, pixel |
| 16138 | | 0.831, shown at level 9.77, step 2, values float32 |
| 16139 | | |
| 16140 | | > rename #34 cryosparc_P4_J287_map_locres_forJ282 |
| 16141 | | |
| 16142 | | > select add #34 |
| 16143 | | |
| 16144 | | 2 models selected |
| 16145 | | |
| 16146 | | > view matrix models #34,1,0,0,-1.0935,0,1,0,0.90366,0,0,1,6.3532 |
| 16147 | | |
| 16148 | | > ui mousemode right "rotate selected models" |
| 16149 | | |
| 16150 | | > view matrix models |
| 16151 | | > #34,0.41458,0.88648,0.20563,-71.892,0.90978,-0.4089,-0.07146,80.866,0.020734,0.2167,-0.97602,247.42 |
| 16152 | | |
| 16153 | | > view matrix models |
| 16154 | | > #34,0.40248,0.89866,0.17442,-67.589,0.91533,-0.39227,-0.091056,80.514,-0.013407,0.1963,-0.98045,255.56 |
| 16155 | | |
| 16156 | | > view matrix models |
| 16157 | | > #34,0.3547,0.92587,0.13022,-58.659,0.93218,-0.33944,-0.12576,75.687,-0.072238,0.166,-0.98348,268.27 |
| 16158 | | |
| 16159 | | > fitmap #34 inMap #33 |
| 16160 | | |
| 16161 | | Fit map cryosparc_P4_J287_map_locres_forJ282 in map CS_P4_J282_sharp_flip.mrc |
| 16162 | | using 40893 points |
| 16163 | | correlation = 0.2997, correlation about mean = 0.02148, overlap = 2955 |
| 16164 | | steps = 140, shift = 9.61, angle = 2.61 degrees |
| 16165 | | |
| 16166 | | Position of cryosparc_P4_J287_map_locres_forJ282 (#34) relative to |
| 16167 | | CS_P4_J282_sharp_flip.mrc (#33) coordinates: |
| 16168 | | Matrix rotation and translation |
| 16169 | | 0.77162333 -0.63598985 0.01069393 118.29154644 |
| 16170 | | -0.63440309 -0.76826072 0.08548800 317.77796206 |
| 16171 | | -0.04615378 -0.07274879 -0.99628182 298.10083492 |
| 16172 | | Axis -0.94106809 0.33808548 0.00943679 |
| 16173 | | Axis point 0.00000000 186.80747823 142.52542945 |
| 16174 | | Rotation angle (degrees) 175.17728056 |
| 16175 | | Shift along axis -1.07117046 |
| 16176 | | |
| 16177 | | |
| 16178 | | > volume #34 level 6.762 |
| 16179 | | |
| 16180 | | > view matrix models |
| 16181 | | > #34,0.42433,0.90126,0.087581,-65.663,0.89997,-0.40908,-0.15069,88.731,-0.099987,0.14276,-0.98469,281 |
| 16182 | | |
| 16183 | | > ui mousemode right "translate selected models" |
| 16184 | | |
| 16185 | | > view matrix models |
| 16186 | | > #34,0.42433,0.90126,0.087581,-62.736,0.89997,-0.40908,-0.15069,86.303,-0.099987,0.14276,-0.98469,272.65 |
| 16187 | | |
| 16188 | | > view matrix models |
| 16189 | | > #34,0.42433,0.90126,0.087581,-62.591,0.89997,-0.40908,-0.15069,86.167,-0.099987,0.14276,-0.98469,272.2 |
| 16190 | | |
| 16191 | | > volume #34 level 6.762 |
| 16192 | | |
| 16193 | | > volume #34 level 6.47 |
| 16194 | | |
| 16195 | | > ui tool show "Surface Color" |
| 16196 | | |
| 16197 | | > color sample #33.1 map #34 palette #ff0000:#ffffff:#0000ff outsideColor |
| 16198 | | > #808080 |
| 16199 | | |
| 16200 | | Map values for surface "surface": minimum 0, mean 4.349, maximum 40.76 |
| 16201 | | |
| 16202 | | > select subtract #34 |
| 16203 | | |
| 16204 | | Nothing selected |
| 16205 | | |
| 16206 | | > hide #!34 models |
| 16207 | | |
| 16208 | | > key red-white-blue :0.0 :20.4 :40.8 showTool true |
| 16209 | | |
| 16210 | | > ui mousemode right "color key" |
| 16211 | | |
| 16212 | | > key red-white-blue :0.0 :20.4 :40.8 white: |
| 16213 | | |
| 16214 | | > key red:0.0 white:20.4 blue:40.8 white: white: |
| 16215 | | |
| 16216 | | > key red:0.0 white:20.4 blue:40.8 white: |
| 16217 | | |
| 16218 | | > key red-white-blue :0.0 :20.4 :40.8 |
| 16219 | | |
| 16220 | | > key red-white-blue :0 :2 :4 showTool true |
| 16221 | | |
| 16222 | | > ui mousemode right "color key" |
| 16223 | | |
| 16224 | | > key red-white-blue :0 :2 :4 white: |
| 16225 | | |
| 16226 | | > key red:0 white:2 blue:4 white: white: |
| 16227 | | |
| 16228 | | > color single #33.1 |
| 16229 | | |
| 16230 | | > color sample #33.1 map #34 palette 0,#ff0000:2,#ffffff:4,#0000ff |
| 16231 | | > outsideColor #808080 |
| 16232 | | |
| 16233 | | Map values for surface "surface": minimum 0, mean 4.349, maximum 40.76 |
| 16234 | | |
| 16235 | | > key red-white-blue :0 :2 :4 showTool true |
| 16236 | | |
| 16237 | | > ui mousemode right "color key" |
| 16238 | | |
| 16239 | | > key red-white-blue :0 :2 :4 showTool true |
| 16240 | | |
| 16241 | | > key red-white-blue :0 :2 :4 white: |
| 16242 | | |
| 16243 | | > key red:0 white:2 blue:4 white: white: |
| 16244 | | |
| 16245 | | > key red:0 white:2 blue:4 white: white:0 |
| 16246 | | |
| 16247 | | > key red:0 white:2 blue:4 white:1 white:0 |
| 16248 | | |
| 16249 | | > key red:0 white:2 blue:2 white:1 white:0 |
| 16250 | | |
| 16251 | | > key red:0 white:3 blue:2 white:1 white:0 |
| 16252 | | |
| 16253 | | > key red:4 white:3 blue:2 white:1 white:0 |
| 16254 | | |
| 16255 | | > key gnbu :4 :3 :2 :1 :0 |
| 16256 | | |
| 16257 | | > key esmfold :4 :3 :2 :1 :0 |
| 16258 | | |
| 16259 | | > key dark2-5 :4 :3 :2 :1 :0 |
| 16260 | | |
| 16261 | | > key pastel1-5 :4 :3 :2 :1 :0 |
| 16262 | | |
| 16263 | | > key blues :4 :3 :2 :1 :0 |
| 16264 | | |
| 16265 | | > key accent-5 :4 :3 :2 :1 :0 |
| 16266 | | |
| 16267 | | > color sample #33.1 map #34 palette 0,#ff0000:2,#ffffff:4,#0000ff |
| 16268 | | > outsideColor #808080 |
| 16269 | | |
| 16270 | | Map values for surface "surface": minimum 0, mean 4.349, maximum 40.76 |
| 16271 | | |
| 16272 | | > color sample #33.1 map #34 palette 0,#ff0000:2,#ffffff:4,#0000ff |
| 16273 | | > outsideColor #808080 |
| 16274 | | |
| 16275 | | Map values for surface "surface": minimum 0, mean 4.349, maximum 40.76 |
| 16276 | | |
| 16277 | | > color sample #33.1 map #34 palette 0,#0000ff:2,#7f7fff:4,#ffffff |
| 16278 | | > outsideColor #808080 |
| 16279 | | |
| 16280 | | Map values for surface "surface": minimum 0, mean 4.349, maximum 40.76 |
| 16281 | | |
| 16282 | | > key blue:0 #7f7fff:2 white:4 showTool true |
| 16283 | | |
| 16284 | | > ui mousemode right "color key" |
| 16285 | | |
| 16286 | | > key blue:0 #7f7fff:2 white:4 showTool true |
| 16287 | | |
| 16288 | | > key blue:0 #7f7fff:2 white:4 white: |
| 16289 | | |
| 16290 | | > key blue:0 #7f7fff:2 white:4 white: white: |
| 16291 | | |
| 16292 | | > color sample #33.1 map #34 palette |
| 16293 | | > 0,#0000ff:1,#7f7fff:2,#ffffff:3,#ff7f7f:4,#ff0000 outsideColor #808080 |
| 16294 | | |
| 16295 | | Map values for surface "surface": minimum 0, mean 4.349, maximum 40.76 |
| 16296 | | |
| 16297 | | > key blue:0 #7f7fff:1 white:2 #ff7f7f:3 red:4 showTool true |
| 16298 | | |
| 16299 | | > ui mousemode right "color key" |
| 16300 | | |
| 16301 | | > color sample #33.1 map #34 palette |
| 16302 | | > 0,#0000ff:1,#7f7fff:2,#ffffff:3,#ff7f7f:4,#ff0000 outsideColor #808080 |
| 16303 | | |
| 16304 | | Map values for surface "surface": minimum 0, mean 4.349, maximum 40.76 |
| 16305 | | |
| 16306 | | > color sample #33.1 map #34 palette |
| 16307 | | > 0,#ff0000:1,#ff7f7f:2,#ffffff:3,#7f7fff:4,#0000ff outsideColor #808080 |
| 16308 | | |
| 16309 | | Map values for surface "surface": minimum 0, mean 4.349, maximum 40.76 |
| 16310 | | |
| 16311 | | > color sample #33.1 map #34 palette |
| 16312 | | > 0,#ff0000:2,#ff7f7f:4,#ffffff:5,#7f7fff:8,#0000ff outsideColor #808080 |
| 16313 | | |
| 16314 | | Map values for surface "surface": minimum 0, mean 4.349, maximum 40.76 |
| 16315 | | |
| 16316 | | > volume #33 level 0.08574 |
| 16317 | | |
| 16318 | | > key red:0 #ff7f7f:2 white:4 #7f7fff:5 blue:8 showTool true |
| 16319 | | |
| 16320 | | > key red:0 #ff7f7f:2 white:4 #7f7fff:6 blue:8 showTool true |
| 16321 | | |
| 16322 | | > color sample #33.1 map #34 palette |
| 16323 | | > 0,#0000ff:2,#7f7fff:4,#ffffff:6,#ff7f7f:8,#ff0000 outsideColor #808080 |
| 16324 | | |
| 16325 | | Map values for surface "surface": minimum 0, mean 4.269, maximum 35.43 |
| 16326 | | |
| 16327 | | > color sample #33.1 map #34 palette |
| 16328 | | > 0,#0000ff:2,#7f7fff:4,#ffffff:6,#ff7f7f:8,#fff6c0 outsideColor #808080 |
| 16329 | | |
| 16330 | | Map values for surface "surface": minimum 0, mean 4.269, maximum 35.43 |
| 16331 | | |
| 16332 | | > key blue:0 #7f7fff:2 white:4 #ff7f7f:6 #fff6c0:8 showTool true |
| 16333 | | |
| 16334 | | > color sample #33.1 map #34 palette |
| 16335 | | > 0,#0000ff:2,#7f7fff:4,#ffffff:6,#ffad7f:8,#fff6c0 outsideColor #808080 |
| 16336 | | |
| 16337 | | Map values for surface "surface": minimum 0, mean 4.269, maximum 35.43 |
| 16338 | | |
| 16339 | | > color sample #33.1 map #34 palette |
| 16340 | | > 0,#0000ff:2,#7f7fff:4,#ffffff:6,#ff967f:8,#fff6c0 outsideColor #808080 |
| 16341 | | |
| 16342 | | Map values for surface "surface": minimum 0, mean 4.269, maximum 35.43 |
| 16343 | | |
| 16344 | | > key blue:0 #7f7fff:2 white:4 #ff967f:6 #fff6c0:8 showTool true |
| 16345 | | |
| 16346 | | > color sample #33.1 map #34 palette |
| 16347 | | > 0,#fff6c0:2,#ff967f:4,#ffffff:6,#7f7fff:8,#0000ff outsideColor #808080 |
| 16348 | | |
| 16349 | | Map values for surface "surface": minimum 0, mean 4.269, maximum 35.43 |
| 16350 | | |
| 16351 | | > color sample #33.1 map #34 palette |
| 16352 | | > 0,#0000ff:2,#7f7fff:4,#ffffff:6,#ff967f:8,#fff6c0 outsideColor #808080 |
| 16353 | | |
| 16354 | | Map values for surface "surface": minimum 0, mean 4.269, maximum 35.43 |
| 16355 | | |
| 16356 | | > key blue:0 #7f7fff:2 white:4 #ff967f:6 #fff6c0:8 showTool true |
| 16357 | | |
| 16358 | | > color sample #33.1 map #34 palette |
| 16359 | | > 0,#00a7ff:2,#7f7fff:4,#ffffff:6,#ff967f:8,#fff6c0 outsideColor #808080 |
| 16360 | | |
| 16361 | | Map values for surface "surface": minimum 0, mean 4.269, maximum 35.43 |
| 16362 | | |
| 16363 | | > key #00a7ff:0 #7f7fff:2 white:4 #ff967f:6 #fff6c0:8 showTool true |
| 16364 | | |
| 16365 | | > color sample #33.1 map #34 palette |
| 16366 | | > 0,#00a7ff:2,#d2a5ff:4,#ffffff:6,#ff967f:8,#fff6c0 outsideColor #808080 |
| 16367 | | |
| 16368 | | Map values for surface "surface": minimum 0, mean 4.269, maximum 35.43 |
| 16369 | | |
| 16370 | | > key #00a7ff:0 #d2a5ff:2 white:4 #ff967f:6 #fff6c0:8 showTool true |
| 16371 | | |
| 16372 | | > save "/Users/peiyuyu/Documents/Goethe |
| 16373 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 16374 | | > F_B1/20260502_SA-F_B1_workflow2.cxs" |
| 16375 | | |
| 16376 | | > open "/Users/peiyuyu/Documents/Goethe |
| 16377 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 16378 | | > F_B1/cryosparc_P4_J288_map_locres.mrc" |
| 16379 | | |
| 16380 | | Opened cryosparc_P4_J288_map_locres.mrc as #36, grid size 320,320,320, pixel |
| 16381 | | 0.831, shown at level 8.93, step 2, values float32 |
| 16382 | | |
| 16383 | | > hide #35 models |
| 16384 | | |
| 16385 | | > show #35 models |
| 16386 | | |
| 16387 | | > hide #!36 models |
| 16388 | | |
| 16389 | | > hide #!33 models |
| 16390 | | |
| 16391 | | > show #!33 models |
| 16392 | | |
| 16393 | | > rename #36 cryosparc_P4_J288_map_locres_forJ285 |
| 16394 | | |
| 16395 | | > hide #!33 models |
| 16396 | | |
| 16397 | | > show #!32 models |
| 16398 | | |
| 16399 | | > hide #35 models |
| 16400 | | |
| 16401 | | > show #!36 models |
| 16402 | | |
| 16403 | | > select add #36 |
| 16404 | | |
| 16405 | | 2 models selected |
| 16406 | | |
| 16407 | | > ui mousemode right "rotate selected models" |
| 16408 | | |
| 16409 | | > view matrix models |
| 16410 | | > #36,0.54984,-0.77769,0.30476,129.28,-0.81459,-0.57994,-0.010221,331.17,0.18469,-0.24264,-0.95237,263.23 |
| 16411 | | |
| 16412 | | > ui mousemode right "translate selected models" |
| 16413 | | |
| 16414 | | > view matrix models |
| 16415 | | > #36,0.54984,-0.77769,0.30476,119.97,-0.81459,-0.57994,-0.010221,318.58,0.18469,-0.24264,-0.95237,280.96 |
| 16416 | | |
| 16417 | | > volume #36 level 6.234 |
| 16418 | | |
| 16419 | | > select subtract #36 |
| 16420 | | |
| 16421 | | Nothing selected |
| 16422 | | |
| 16423 | | > ui tool show "Surface Color" |
| 16424 | | |
| 16425 | | > color sample #32.1 map #36 palette |
| 16426 | | > 0,#00a7ff:2,#d2a5ff:4,#ffffff:6,#ff967f:8,#fff6c0 outsideColor #808080 |
| 16427 | | |
| 16428 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 16429 | | |
| 16430 | | > hide #!36 models |
| 16431 | | |
| 16432 | | > show #!33 models |
| 16433 | | |
| 16434 | | > hide #!33 models |
| 16435 | | |
| 16436 | | > show #!33 models |
| 16437 | | |
| 16438 | | > hide #!32 models |
| 16439 | | |
| 16440 | | > show #!32 models |
| 16441 | | |
| 16442 | | > hide #!33 models |
| 16443 | | |
| 16444 | | > key #00a7ff:0 #d2a5ff:2 white:4 #ff967f:6 #fff6c0:8 showTool true |
| 16445 | | |
| 16446 | | > show #!36 models |
| 16447 | | |
| 16448 | | > hide #!36 models |
| 16449 | | |
| 16450 | | > key #00a7ff:0 #d2a5ff:2 white:4 #ff967f:6 #fff6c0:8 white: |
| 16451 | | |
| 16452 | | > key #00a7ff:0 #d2a5ff:2 white:4 #ff967f:6 #fff6c0:8 |
| 16453 | | |
| 16454 | | Fetching compressed palette custom from |
| 16455 | | https://www.colourlovers.com/api/palettes?keywords=custom&format=json&numResults=100 |
| 16456 | | Could not find palette custom at COLOURlovers.com using keyword search |
| 16457 | | |
| 16458 | | > color sample #32.1 map #36 palette |
| 16459 | | > 0,#00a7ff:2,#d2a5ff:4,#ffffff:6,#ff967f:8,#fff6c0 outsideColor #808080 |
| 16460 | | |
| 16461 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 16462 | | |
| 16463 | | > key #00a7ff:0 #d2a5ff:2 white:4 #ff967f:6 #fff6c0:8 showTool true |
| 16464 | | |
| 16465 | | > hide #!32 models |
| 16466 | | |
| 16467 | | > show #35 models |
| 16468 | | |
| 16469 | | > show #!32 models |
| 16470 | | |
| 16471 | | > select add #35 |
| 16472 | | |
| 16473 | | 1 model selected |
| 16474 | | |
| 16475 | | > view matrix models #35,1,0,0,-1.208,0,1,0,-1.3654,0,0,1,1.1486 |
| 16476 | | |
| 16477 | | > select subtract #35 |
| 16478 | | |
| 16479 | | Nothing selected |
| 16480 | | |
| 16481 | | > select add #35 |
| 16482 | | |
| 16483 | | 1 model selected |
| 16484 | | |
| 16485 | | > select subtract #35 |
| 16486 | | |
| 16487 | | Nothing selected |
| 16488 | | |
| 16489 | | > key #00a7ff:0 #d2a5ff:2 white:4 #ff967f:6 #fff6c0:8 showTool true |
| 16490 | | |
| 16491 | | > ui mousemode right "color key" |
| 16492 | | |
| 16493 | | > select add #35 |
| 16494 | | |
| 16495 | | 1 model selected |
| 16496 | | |
| 16497 | | > show #35 |
| 16498 | | |
| 16499 | | > view W35 |
| 16500 | | |
| 16501 | | Expected an objects specifier or a view name or a keyword |
| 16502 | | |
| 16503 | | > view #35 |
| 16504 | | |
| 16505 | | No displayed objects specified. |
| 16506 | | |
| 16507 | | > close #35 |
| 16508 | | |
| 16509 | | > key #00a7ff:0 #d2a5ff:2 white:4 #ff967f:6 #fff6c0:8 showTool true |
| 16510 | | |
| 16511 | | > ui mousemode right "color key" |
| 16512 | | |
| 16513 | | > hide #!32 models |
| 16514 | | |
| 16515 | | > show #!33 models |
| 16516 | | |
| 16517 | | > hide #!33 models |
| 16518 | | |
| 16519 | | > show #!33 models |
| 16520 | | |
| 16521 | | > hide #!33 models |
| 16522 | | |
| 16523 | | > show #!33 models |
| 16524 | | |
| 16525 | | > volume #33 level 0.06991 |
| 16526 | | |
| 16527 | | > hide #!33 models |
| 16528 | | |
| 16529 | | > show #!32 models |
| 16530 | | |
| 16531 | | > save "/Users/peiyuyu/Documents/Goethe |
| 16532 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 16533 | | > F_B1/20260502_SA-F_B1_workflow2.cxs" |
| 16534 | | |
| 16535 | | > color sample #32.1 map #36 palette |
| 16536 | | > 0,#00a7ff:2,#99bfe5:4,#ffffff:6,#ff967f:8,#fff6c0 outsideColor #808080 |
| 16537 | | |
| 16538 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 16539 | | |
| 16540 | | > key #00a7ff:0 #99bfe5:2 white:4 #fff6c0:6 #ff967f:8 showTool true |
| 16541 | | |
| 16542 | | > ui mousemode right "color key" |
| 16543 | | |
| 16544 | | > color sample #32.1 map #36 palette |
| 16545 | | > 0,#00a7ff:2,#99bfe5:4,#ffffff:6,#fff6c0:8,#ff967f outsideColor #808080 |
| 16546 | | |
| 16547 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 16548 | | |
| 16549 | | > color sample #32.1 map #36 palette |
| 16550 | | > 0,#d2a5ff:2,#99bfe5:4,#ffffff:6,#fff6c0:8,#ff967f outsideColor #808080 |
| 16551 | | |
| 16552 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 16553 | | |
| 16554 | | > key #d2a5ff:0 #99bfe5:2 white:4 #fff6c0:6 #ff967f:8 showTool true |
| 16555 | | |
| 16556 | | > view #35 |
| 16557 | | |
| 16558 | | No objects specified. |
| 16559 | | |
| 16560 | | > color sample #32.1 map #36 palette |
| 16561 | | > 0,#e7c4ff:2,#99bfe5:4,#ffffff:6,#fff6c0:8,#ff967f outsideColor #808080 |
| 16562 | | |
| 16563 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 16564 | | |
| 16565 | | > key #e7c4ff:0 #99bfe5:2 white:4 #fff6c0:6 #ff967f:8 showTool true |
| 16566 | | |
| 16567 | | > save "/Users/peiyuyu/Documents/Goethe |
| 16568 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 16569 | | > F_B1/20260502_SA-F_B1_workflow2.cxs" |
| 16570 | | |
| 16571 | | > color sample #32.1 map #36 palette |
| 16572 | | > 0,#e7c4ff:2,#99bfe5:4,#5da0ff:6,#fff6c0:8,#ff967f outsideColor #808080 |
| 16573 | | |
| 16574 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 16575 | | |
| 16576 | | > color sample #32.1 map #36 palette |
| 16577 | | > 0,#e7c4ff:2,#e0bfe8:4,#99bfe5:6,#fff6c0:8,#ff967f outsideColor #808080 |
| 16578 | | |
| 16579 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 16580 | | |
| 16581 | | > color sample #32.1 map #36 palette |
| 16582 | | > 0,#e7c4ff:2,#e0dee8:4,#99bfe5:6,#fff6c0:8,#ff967f outsideColor #808080 |
| 16583 | | |
| 16584 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 16585 | | |
| 16586 | | > color sample #32.1 map #36 palette |
| 16587 | | > 0,#e7c4ff:2,#e0dee8:4,#99bfe5:6,#fff6c0:8,#ff967f outsideColor #808080 |
| 16588 | | |
| 16589 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 16590 | | |
| 16591 | | > color sample #32.1 map #36 palette |
| 16592 | | > 0,#e7c4ff:2,#6676ed:4,#99bfe5:6,#fff6c0:8,#ff967f outsideColor #808080 |
| 16593 | | |
| 16594 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 16595 | | |
| 16596 | | > color sample #32.1 map #36 palette |
| 16597 | | > 0,#e7c4ff:2,#6676ed:4,#99bfe5:6,#fff6c0:8,#f4fff3 outsideColor #808080 |
| 16598 | | |
| 16599 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 16600 | | |
| 16601 | | > color sample #32.1 map #36 palette |
| 16602 | | > 0,#e7c4ff:2,#99bfe5:4,#99bfe5:6,#fff6c0:8,#f4fff3 outsideColor #808080 |
| 16603 | | |
| 16604 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 16605 | | |
| 16606 | | > color sample #32.1 map #36 palette |
| 16607 | | > 0,#e7c4ff:2,#99bfe5:4,#6676ed:6,#fff6c0:8,#f4fff3 outsideColor #808080 |
| 16608 | | |
| 16609 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 16610 | | |
| 16611 | | > color sample #32.1 map #36 palette |
| 16612 | | > 0,#e7c4ff:2,#99bfe5:4,#99bfe5:6,#fff6c0:8,#f4fff3 outsideColor #808080 |
| 16613 | | |
| 16614 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 16615 | | |
| 16616 | | > color sample #32.1 map #36 palette |
| 16617 | | > 0,#e7c4ff:2,#799bea:4,#99bfe5:6,#fff6c0:8,#f4fff3 outsideColor #808080 |
| 16618 | | |
| 16619 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 16620 | | |
| 16621 | | > color sample #32.1 map #36 palette |
| 16622 | | > 0,#e7c4ff:2,#5756ea:4,#99bfe5:6,#fff6c0:8,#f4fff3 outsideColor #808080 |
| 16623 | | |
| 16624 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 16625 | | |
| 16626 | | > color sample #32.1 map #36 palette |
| 16627 | | > 0,#e7c4ff:2,#527dea:4,#99bfe5:6,#fff6c0:8,#f4fff3 outsideColor #808080 |
| 16628 | | |
| 16629 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 16630 | | |
| 16631 | | > color sample #32.1 map #36 palette |
| 16632 | | > 0,#ffdedd:2,#527dea:4,#99bfe5:6,#fff6c0:8,#f4fff3 outsideColor #808080 |
| 16633 | | |
| 16634 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 16635 | | |
| 16636 | | > color sample #32.1 map #36 palette |
| 16637 | | > 0,#ffc9a3:2,#527dea:4,#99bfe5:6,#fff6c0:8,#f4fff3 outsideColor #808080 |
| 16638 | | |
| 16639 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 16640 | | |
| 16641 | | > color sample #32.1 map #36 palette |
| 16642 | | > 0,#ffb17f:2,#527dea:4,#99bfe5:6,#fff6c0:8,#f4fff3 outsideColor #808080 |
| 16643 | | |
| 16644 | | Map values for surface "surface": minimum 0, mean 4.33, maximum 41.38 |
| 16645 | | |
| 16646 | | > key #ffb17f:0 #527dea:2 #99bfe5:4 #fff6c0:6 #f4fff3:8 showTool true |
| 16647 | | |
| 16648 | | > ui mousemode right "color key" |
| 16649 | | |
| 16650 | | > save "/Users/peiyuyu/Documents/Goethe |
| 16651 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 16652 | | > F_B1/20260502_SA-F_B1_workflow3.cxs" |
| 16653 | | |
| 16654 | | > hide #!32 models |
| 16655 | | |
| 16656 | | > show #!33 models |
| 16657 | | |
| 16658 | | > hide #!33 models |
| 16659 | | |
| 16660 | | > show #!32 models |
| 16661 | | |
| 16662 | | > hide #!32 models |
| 16663 | | |
| 16664 | | > show #!33 models |
| 16665 | | |
| 16666 | | > hide #!33 models |
| 16667 | | |
| 16668 | | > show #!32 models |
| 16669 | | |
| 16670 | | > volume #34 step 1 |
| 16671 | | |
| 16672 | | > volume #36 step 1 |
| 16673 | | |
| 16674 | | > show #!36 models |
| 16675 | | |
| 16676 | | > volume flip #36 |
| 16677 | | |
| 16678 | | Opened cryosparc_P4_J288_map_locres_forJ285 z flip as #38, grid size |
| 16679 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 16680 | | |
| 16681 | | > hide #37 models |
| 16682 | | |
| 16683 | | > ui mousemode right "rotate selected models" |
| 16684 | | |
| 16685 | | > hide #!32 models |
| 16686 | | |
| 16687 | | > show #!32 models |
| 16688 | | |
| 16689 | | > hide #!38 models |
| 16690 | | |
| 16691 | | > show #!38 models |
| 16692 | | |
| 16693 | | > select add #38 |
| 16694 | | |
| 16695 | | 2 models selected |
| 16696 | | |
| 16697 | | > view matrix models |
| 16698 | | > #38,-0.22965,0.94651,-0.22667,61.099,-0.95753,-0.26145,-0.12161,309.34,-0.17437,0.18912,0.96635,13.289 |
| 16699 | | |
| 16700 | | > view matrix models |
| 16701 | | > #38,-0.47581,-0.84028,-0.25987,345.82,0.86453,-0.50117,0.037622,69.811,-0.16185,-0.20677,0.96491,66.334 |
| 16702 | | |
| 16703 | | > view matrix models |
| 16704 | | > #38,-0.72836,0.6263,-0.27792,180.88,-0.55109,-0.77649,-0.30556,349.01,-0.40718,-0.069403,0.91071,88.496 |
| 16705 | | |
| 16706 | | > view matrix models |
| 16707 | | > #38,-0.422,0.88275,-0.20657,93.707,-0.7937,-0.46985,-0.38638,351.04,-0.43814,0.0009021,0.89891,84.657 |
| 16708 | | |
| 16709 | | > fitmap #38 inMap #32 |
| 16710 | | |
| 16711 | | Fit map cryosparc_P4_J288_map_locres_forJ285 z flip in map |
| 16712 | | CS_P4_J285_sharp_flip.mrc using 1112104 points |
| 16713 | | correlation = 0.2781, correlation about mean = -0.02765, overlap = 7.665e+04 |
| 16714 | | steps = 172, shift = 16.7, angle = 11.9 degrees |
| 16715 | | |
| 16716 | | Position of cryosparc_P4_J288_map_locres_forJ285 z flip (#38) relative to |
| 16717 | | CS_P4_J285_sharp_flip.mrc (#32) coordinates: |
| 16718 | | Matrix rotation and translation |
| 16719 | | -0.15731459 0.96448973 -0.21215945 50.76791567 |
| 16720 | | -0.83839054 -0.24396591 -0.48742375 323.17714111 |
| 16721 | | -0.52187488 0.10119361 0.84699849 86.68679965 |
| 16722 | | Axis 0.30630695 0.16117090 -0.93818974 |
| 16723 | | Axis point 169.99659509 146.99779714 0.00000000 |
| 16724 | | Rotation angle (degrees) 106.08964492 |
| 16725 | | Shift along axis -13.69134858 |
| 16726 | | |
| 16727 | | |
| 16728 | | > ui mousemode right "translate selected models" |
| 16729 | | |
| 16730 | | > view matrix models |
| 16731 | | > #38,-0.29391,0.88572,-0.35933,106.9,-0.78003,-0.43953,-0.4454,363.34,-0.55243,0.14938,0.82006,93.87 |
| 16732 | | |
| 16733 | | > ui mousemode right "rotate selected models" |
| 16734 | | |
| 16735 | | > volume #38 level 4.082 |
| 16736 | | |
| 16737 | | > view matrix models |
| 16738 | | > #38,-0.44764,0.81063,-0.37749,140.89,-0.70715,-0.57929,-0.40543,367.19,-0.54732,0.085454,0.83255,100.3 |
| 16739 | | |
| 16740 | | > ui mousemode right "translate selected models" |
| 16741 | | |
| 16742 | | [Repeated 1 time(s)] |
| 16743 | | |
| 16744 | | > view matrix models |
| 16745 | | > #38,-0.44764,0.81063,-0.37749,135.23,-0.70715,-0.57929,-0.40543,367.93,-0.54732,0.085454,0.83255,97.332 |
| 16746 | | |
| 16747 | | > view matrix models |
| 16748 | | > #38,-0.44764,0.81063,-0.37749,134.86,-0.70715,-0.57929,-0.40543,366.07,-0.54732,0.085454,0.83255,96.949 |
| 16749 | | |
| 16750 | | > select subtract #38 |
| 16751 | | |
| 16752 | | Nothing selected |
| 16753 | | |
| 16754 | | > hide #!38 models |
| 16755 | | |
| 16756 | | > color sample #32.1 map #38 palette |
| 16757 | | > 0,#ffb17f:2,#527dea:4,#99bfe5:6,#fff6c0:8,#f4fff3 outsideColor #808080 |
| 16758 | | |
| 16759 | | Map values for surface "surface": minimum 0, mean 4.286, maximum 42.51 |
| 16760 | | |
| 16761 | | > open "/Users/peiyuyu/Documents/Goethe |
| 16762 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 16763 | | > F_B1/CS_P4_J282_sharp_flip.mrc" |
| 16764 | | |
| 16765 | | Opened CS_P4_J282_sharp_flip.mrc as #39, grid size 320,320,320, pixel 0.831, |
| 16766 | | shown at level 0.0392, step 2, values float32 |
| 16767 | | |
| 16768 | | > hide #!32 models |
| 16769 | | |
| 16770 | | > close #39 |
| 16771 | | |
| 16772 | | > open "/Users/peiyuyu/Documents/Goethe |
| 16773 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 16774 | | > F_B1/cryosparc_P4_J282_005_volume_map_sharp.mrc" |
| 16775 | | |
| 16776 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc as #35, grid size |
| 16777 | | 320,320,320, pixel 0.831, shown at level 0.0392, step 2, values float32 |
| 16778 | | |
| 16779 | | > open "/Users/peiyuyu/Documents/Goethe |
| 16780 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 16781 | | > F_B1/cryosparc_P4_J287_map_locres.mrc" |
| 16782 | | |
| 16783 | | Opened cryosparc_P4_J287_map_locres_forJ282 as #39, grid size 320,320,320, |
| 16784 | | pixel 0.831, shown at level 9.77, step 2, values float32 |
| 16785 | | |
| 16786 | | > volume #39 level 4.139 |
| 16787 | | |
| 16788 | | > open "/Users/peiyuyu/Documents/Goethe |
| 16789 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 16790 | | > F_B1/cryosparc_P4_J287_map_locres.mrc" |
| 16791 | | |
| 16792 | | Opened cryosparc_P4_J287_map_locres_forJ282 as #40, grid size 320,320,320, |
| 16793 | | pixel 0.831, shown at level 9.77, step 2, values float32 |
| 16794 | | |
| 16795 | | > close #40 |
| 16796 | | |
| 16797 | | > color sample #35.1 map #39 palette |
| 16798 | | > 0,#ffb17f:2,#527dea:4,#99bfe5:6,#fff6c0:8,#f4fff3 outsideColor #808080 |
| 16799 | | |
| 16800 | | Map values for surface "surface": minimum 0, mean 4.417, maximum 39.82 |
| 16801 | | |
| 16802 | | > hide #!39 models |
| 16803 | | |
| 16804 | | > volume #35 step 1 |
| 16805 | | |
| 16806 | | > volume #35 level 0.06749 |
| 16807 | | |
| 16808 | | > color sample #35.1 map #39 palette |
| 16809 | | > 0,#ffb17f:1,#527dea:2,#99bfe5:3,#fff6c0:4,#f4fff3 outsideColor #808080 |
| 16810 | | |
| 16811 | | Map values for surface "surface": minimum 3.044, mean 3.567, maximum 40.68 |
| 16812 | | |
| 16813 | | > color sample #35.1 map #39 palette |
| 16814 | | > 0,#ffb17f:2,#527dea:3,#99bfe5:4,#fff6c0:5,#f4fff3 outsideColor #808080 |
| 16815 | | |
| 16816 | | Map values for surface "surface": minimum 3.044, mean 3.567, maximum 40.68 |
| 16817 | | |
| 16818 | | > color sample #35.1 map #39 palette |
| 16819 | | > 2,#ffb17f:3,#527dea:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 16820 | | |
| 16821 | | Map values for surface "surface": minimum 3.044, mean 3.567, maximum 40.68 |
| 16822 | | |
| 16823 | | > close #32 |
| 16824 | | |
| 16825 | | > close #33 |
| 16826 | | |
| 16827 | | > save "/Users/peiyuyu/Documents/Goethe |
| 16828 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 16829 | | > F_B1/20260503_SA-F_B1_workflow.cxs" |
| 16830 | | |
| 16831 | | > close #39 |
| 16832 | | |
| 16833 | | > close #38 |
| 16834 | | |
| 16835 | | > close #36 |
| 16836 | | |
| 16837 | | > close #35 |
| 16838 | | |
| 16839 | | > show #!34 models |
| 16840 | | |
| 16841 | | > hide #!34 models |
| 16842 | | |
| 16843 | | > close #34 |
| 16844 | | |
| 16845 | | > open "/Users/peiyuyu/Documents/Goethe |
| 16846 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 16847 | | > F_B1/cryosparc_P4_J282_005_volume_map_sharp.mrc" |
| 16848 | | |
| 16849 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc as #32, grid size |
| 16850 | | 320,320,320, pixel 0.831, shown at level 0.0392, step 2, values float32 |
| 16851 | | |
| 16852 | | > open "/Users/peiyuyu/Documents/Goethe |
| 16853 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 16854 | | > F_B1/cryosparc_P4_J285_006_volume_map_sharp.mrc" |
| 16855 | | |
| 16856 | | Opened cryosparc_P4_J285_006_volume_map_sharp.mrc as #33, grid size |
| 16857 | | 320,320,320, pixel 0.831, shown at level 0.0363, step 2, values float32 |
| 16858 | | |
| 16859 | | > open "/Users/peiyuyu/Documents/Goethe |
| 16860 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 16861 | | > F_B1/cryosparc_P4_J287_map_locres.mrc" |
| 16862 | | |
| 16863 | | Opened cryosparc_P4_J287_map_locres.mrc as #34, grid size 320,320,320, pixel |
| 16864 | | 0.831, shown at level 9.77, step 2, values float32 |
| 16865 | | |
| 16866 | | > open "/Users/peiyuyu/Documents/Goethe |
| 16867 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 16868 | | > F_B1/cryosparc_P4_J288_map_locres.mrc" |
| 16869 | | |
| 16870 | | Opened cryosparc_P4_J288_map_locres.mrc as #35, grid size 320,320,320, pixel |
| 16871 | | 0.831, shown at level 8.93, step 2, values float32 |
| 16872 | | |
| 16873 | | > rename #37 Color key id #36 |
| 16874 | | |
| 16875 | | Expected a keyword |
| 16876 | | |
| 16877 | | > rename #37 Color_key id #36 |
| 16878 | | |
| 16879 | | Cannot change id of 2D overlay models (Color_key #37) |
| 16880 | | |
| 16881 | | > hide #!33 models |
| 16882 | | |
| 16883 | | > hide #!35 models |
| 16884 | | |
| 16885 | | > volume #34 level 6.298 |
| 16886 | | |
| 16887 | | > volume #34 level 3.171 |
| 16888 | | |
| 16889 | | > volume #34 level 3.779 |
| 16890 | | |
| 16891 | | > hide #!34 models |
| 16892 | | |
| 16893 | | > ui tool show "Surface Color" |
| 16894 | | |
| 16895 | | No surface chosen for coloring |
| 16896 | | |
| 16897 | | > show #!34 models |
| 16898 | | |
| 16899 | | > hide #!34 models |
| 16900 | | |
| 16901 | | > color sample #32.1 map #34 palette |
| 16902 | | > 2,#ffb17f:3,#527dea:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 16903 | | |
| 16904 | | Map values for surface "surface": minimum 0, mean 4.417, maximum 39.82 |
| 16905 | | |
| 16906 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 16907 | | |
| 16908 | | > ui mousemode right "color key" |
| 16909 | | |
| 16910 | | Could not find palette custom at COLOURlovers.com using keyword search |
| 16911 | | |
| 16912 | | > show #37 models |
| 16913 | | |
| 16914 | | > key pos 0.558738,0.385722 size 0.0255738,0.0868715 |
| 16915 | | |
| 16916 | | > key pos 0.723062,0.0751149 |
| 16917 | | |
| 16918 | | > key pos 0.862303,0.0795068 |
| 16919 | | |
| 16920 | | > key pos 0.846838,0.0779833 |
| 16921 | | |
| 16922 | | > select add #37 |
| 16923 | | |
| 16924 | | 1 model selected |
| 16925 | | |
| 16926 | | > key pos 0.819995,0.0796645 |
| 16927 | | |
| 16928 | | > key pos 0.812107,0.0767711 |
| 16929 | | |
| 16930 | | > key pos 0.825223,0.13099 |
| 16931 | | |
| 16932 | | > select subtract #37 |
| 16933 | | |
| 16934 | | Nothing selected |
| 16935 | | |
| 16936 | | > undo |
| 16937 | | |
| 16938 | | [Repeated 3 time(s)] |
| 16939 | | |
| 16940 | | > color sample #32.1 map #34 palette |
| 16941 | | > 2,#ffb17f:3,#527dea:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 16942 | | |
| 16943 | | Map values for surface "surface": minimum 0, mean 4.417, maximum 39.82 |
| 16944 | | |
| 16945 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 16946 | | |
| 16947 | | > show #37 models |
| 16948 | | |
| 16949 | | > select add #37 |
| 16950 | | |
| 16951 | | 1 model selected |
| 16952 | | |
| 16953 | | > view matrix models |
| 16954 | | > #37,0.70959,0.62592,0.32358,0.61324,-0.29635,0.68175,-0.66888,-0.0021786,-0.63926,0.37874,0.66925,0.69431 |
| 16955 | | |
| 16956 | | > close #37 |
| 16957 | | |
| 16958 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 16959 | | |
| 16960 | | > ui mousemode right "color key" |
| 16961 | | |
| 16962 | | > hide #!32 models |
| 16963 | | |
| 16964 | | > show #!32 models |
| 16965 | | |
| 16966 | | > volume #32 level 0.04587 |
| 16967 | | |
| 16968 | | > volume #32 level 0.04884 |
| 16969 | | |
| 16970 | | > key pos 0.437353,0.539212 size 0.000454748,0.0481037 |
| 16971 | | |
| 16972 | | > hide #36 models |
| 16973 | | |
| 16974 | | > show #36 models |
| 16975 | | |
| 16976 | | > close #36 |
| 16977 | | |
| 16978 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 16979 | | |
| 16980 | | > ui mousemode right "color key" |
| 16981 | | |
| 16982 | | > ui mousemode right translate |
| 16983 | | |
| 16984 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 16985 | | |
| 16986 | | [Repeated 1 time(s)] |
| 16987 | | |
| 16988 | | > ui mousemode right "color key" |
| 16989 | | |
| 16990 | | > ui mousemode right translate |
| 16991 | | |
| 16992 | | > ui mousemode middle 'color key' |
| 16993 | | |
| 16994 | | > key border false |
| 16995 | | |
| 16996 | | > key border true |
| 16997 | | |
| 16998 | | > key borderWidth 1.0 |
| 16999 | | |
| 17000 | | > key borderWidth 0.0 |
| 17001 | | |
| 17002 | | > key borderWidth 1.0 |
| 17003 | | |
| 17004 | | > key colorTreatment distinct |
| 17005 | | |
| 17006 | | > key colorTreatment blended |
| 17007 | | |
| 17008 | | > ui mousemode middle translate |
| 17009 | | |
| 17010 | | > ui mousemode middle "color key" |
| 17011 | | |
| 17012 | | > key borderColor #bfbfbf |
| 17013 | | |
| 17014 | | > key borderColor default |
| 17015 | | |
| 17016 | | > key borderColor #bfbfbf |
| 17017 | | |
| 17018 | | > key borderColor #030303 |
| 17019 | | |
| 17020 | | > key borderColor #020202 |
| 17021 | | |
| 17022 | | > key borderColor #010202 |
| 17023 | | |
| 17024 | | > key borderColor black |
| 17025 | | |
| 17026 | | > key labelColor #bfbfbf |
| 17027 | | |
| 17028 | | > key labelColor default |
| 17029 | | |
| 17030 | | > key fontSize 23 |
| 17031 | | |
| 17032 | | > key fontSize 22 |
| 17033 | | |
| 17034 | | > key fontSize 21 |
| 17035 | | |
| 17036 | | > key fontSize 20 |
| 17037 | | |
| 17038 | | > key fontSize 21 |
| 17039 | | |
| 17040 | | > key fontSize 22 |
| 17041 | | |
| 17042 | | > key fontSize 23 |
| 17043 | | |
| 17044 | | > key fontSize 24 |
| 17045 | | |
| 17046 | | > key bold true |
| 17047 | | |
| 17048 | | > key italic true |
| 17049 | | |
| 17050 | | > key italic false |
| 17051 | | |
| 17052 | | > key numericLabelSpacing equal |
| 17053 | | |
| 17054 | | > key numericLabelSpacing proportional |
| 17055 | | |
| 17056 | | > key labelSide left/top |
| 17057 | | |
| 17058 | | > key labelSide right/bottom |
| 17059 | | |
| 17060 | | > key justification left |
| 17061 | | |
| 17062 | | > key labelSide right/bottom |
| 17063 | | |
| 17064 | | > key justification decimal |
| 17065 | | |
| 17066 | | > key justification right |
| 17067 | | |
| 17068 | | > key justification left |
| 17069 | | |
| 17070 | | > key labelOffset 1.0 |
| 17071 | | |
| 17072 | | > key labelOffset 2.0 |
| 17073 | | |
| 17074 | | > key labelOffset 3.0 |
| 17075 | | |
| 17076 | | > key labelOffset 4.0 |
| 17077 | | |
| 17078 | | > key labelOffset 5.0 |
| 17079 | | |
| 17080 | | > key labelOffset 4.0 |
| 17081 | | |
| 17082 | | > key labelOffset 3.0 |
| 17083 | | |
| 17084 | | > key labelOffset 2.0 |
| 17085 | | |
| 17086 | | > key ticks true |
| 17087 | | |
| 17088 | | > key ticks false |
| 17089 | | |
| 17090 | | > key size 0.26000,0.05000 |
| 17091 | | |
| 17092 | | > key size 0.27000,0.05000 |
| 17093 | | |
| 17094 | | > key size 0.26000,0.05000 |
| 17095 | | |
| 17096 | | > key size 0.25000,0.05000 |
| 17097 | | |
| 17098 | | > key size 0.24000,0.05000 |
| 17099 | | |
| 17100 | | > key size 0.23000,0.05000 |
| 17101 | | |
| 17102 | | > key size 0.22000,0.05000 |
| 17103 | | |
| 17104 | | > key size 0.22000,0.04000 |
| 17105 | | |
| 17106 | | > key size 0.21000,0.04000 |
| 17107 | | |
| 17108 | | > key size 0.20000,0.04000 |
| 17109 | | |
| 17110 | | > key size 0.19000,0.04000 |
| 17111 | | |
| 17112 | | > key bold false |
| 17113 | | |
| 17114 | | > key borderWidth 2.0 |
| 17115 | | |
| 17116 | | > key border false |
| 17117 | | |
| 17118 | | > key border true |
| 17119 | | |
| 17120 | | > key size 0.18000,0.04000 |
| 17121 | | |
| 17122 | | > key size 0.17000,0.04000 |
| 17123 | | |
| 17124 | | > key size 0.17000,0.03000 |
| 17125 | | |
| 17126 | | > key size 0.17000,0.02000 |
| 17127 | | |
| 17128 | | > key size 0.16000,0.02000 |
| 17129 | | |
| 17130 | | > key size 0.15000,0.02000 |
| 17131 | | |
| 17132 | | > key size 0.14000,0.02000 |
| 17133 | | |
| 17134 | | > key size 0.15000,0.02000 |
| 17135 | | |
| 17136 | | > key size 0.14000,0.02000 |
| 17137 | | |
| 17138 | | > key size 0.14000,0.01000 |
| 17139 | | |
| 17140 | | > key size 0.14000,0.02000 |
| 17141 | | |
| 17142 | | > key size 0.14000,0.01000 |
| 17143 | | |
| 17144 | | > key size 0.14000,0.02000 |
| 17145 | | |
| 17146 | | > key size 0.14000,0.03000 |
| 17147 | | |
| 17148 | | > key size 0.14000,0.04000 |
| 17149 | | |
| 17150 | | > key size 0.14000,0.03000 |
| 17151 | | |
| 17152 | | > key bold true |
| 17153 | | |
| 17154 | | > save CS_P4_J282_locres_sharp.png supersample 3 transparentBackground true |
| 17155 | | |
| 17156 | | > hide #!32 models |
| 17157 | | |
| 17158 | | > show #!33 models |
| 17159 | | |
| 17160 | | > show #!35 models |
| 17161 | | |
| 17162 | | > volume #33 level 0.0299 |
| 17163 | | |
| 17164 | | > undo |
| 17165 | | |
| 17166 | | > show #!35 models |
| 17167 | | |
| 17168 | | > volume #35 level 3.774 |
| 17169 | | |
| 17170 | | > hide #!35 models |
| 17171 | | |
| 17172 | | > color sample #33.1 map #35 palette |
| 17173 | | > 2,#ffb17f:3,#527dea:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 17174 | | |
| 17175 | | Map values for surface "surface": minimum 0, mean 4.383, maximum 41.2 |
| 17176 | | |
| 17177 | | > volume #33 level 0.03991 |
| 17178 | | |
| 17179 | | > volume #33 level 0.049 |
| 17180 | | |
| 17181 | | > volume #33 level 0.06811 |
| 17182 | | |
| 17183 | | > volume #33 level 0.05628 |
| 17184 | | |
| 17185 | | > volume #33 level 0.05719 |
| 17186 | | |
| 17187 | | > ui mousemode right translate |
| 17188 | | |
| 17189 | | [Repeated 1 time(s)] |
| 17190 | | |
| 17191 | | > select add #37 |
| 17192 | | |
| 17193 | | 1 model selected |
| 17194 | | |
| 17195 | | > ui mousemode right translate |
| 17196 | | |
| 17197 | | > select subtract #37 |
| 17198 | | |
| 17199 | | Nothing selected |
| 17200 | | |
| 17201 | | > view #33 |
| 17202 | | |
| 17203 | | > volume #33 level 0.05264 |
| 17204 | | |
| 17205 | | > volume #33 level 0.05082 |
| 17206 | | |
| 17207 | | > save CS_P4_J285_NU_sharp.png supersample 3 transparentBackground true |
| 17208 | | |
| 17209 | | > save CS_P4_J285_locres_sharp.png supersample 3 transparentBackground true |
| 17210 | | |
| 17211 | | > hide #!33 models |
| 17212 | | |
| 17213 | | > show #!32 models |
| 17214 | | |
| 17215 | | > volume #32 level 0.05478 |
| 17216 | | |
| 17217 | | > save CS_P4_J282_locres_sharp.png supersample 3 transparentBackground true |
| 17218 | | |
| 17219 | | > save "/Users/peiyuyu/Documents/Goethe |
| 17220 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 17221 | | > F_B1/20260503_SA-F_B1_workflow1.cxs" |
| 17222 | | |
| 17223 | | > show #!33 models |
| 17224 | | |
| 17225 | | > hide #!33 models |
| 17226 | | |
| 17227 | | > volume flip #32 |
| 17228 | | |
| 17229 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc z flip as #38, grid size |
| 17230 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 17231 | | |
| 17232 | | > color sample #38.1 map #34 palette |
| 17233 | | > 2,#ffb17f:3,#527dea:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 17234 | | |
| 17235 | | Map values for surface "surface": minimum 0, mean 4.643, maximum 46.37 |
| 17236 | | |
| 17237 | | > select add #38 |
| 17238 | | |
| 17239 | | 2 models selected |
| 17240 | | |
| 17241 | | > select subtract #38 |
| 17242 | | |
| 17243 | | Nothing selected |
| 17244 | | |
| 17245 | | > show #!34 models |
| 17246 | | |
| 17247 | | > hide #!34 models |
| 17248 | | |
| 17249 | | > show #!32 models |
| 17250 | | |
| 17251 | | > select add #38 |
| 17252 | | |
| 17253 | | 2 models selected |
| 17254 | | |
| 17255 | | > ui mousemode right "rotate selected models" |
| 17256 | | |
| 17257 | | > view matrix models |
| 17258 | | > #38,0.96218,0.27239,0.0026401,-33.277,0.27218,-0.96095,-0.049909,243.62,-0.011058,0.04874,-0.99875,256.5 |
| 17259 | | |
| 17260 | | > fitmap #38 inMap #32 |
| 17261 | | |
| 17262 | | Fit map cryosparc_P4_J282_005_volume_map_sharp.mrc z flip in map |
| 17263 | | cryosparc_P4_J282_005_volume_map_sharp.mrc using 208382 points |
| 17264 | | correlation = 0.3998, correlation about mean = 0.04433, overlap = 423.6 |
| 17265 | | steps = 132, shift = 4.37, angle = 6.4 degrees |
| 17266 | | |
| 17267 | | Position of cryosparc_P4_J282_005_volume_map_sharp.mrc z flip (#38) relative |
| 17268 | | to cryosparc_P4_J282_005_volume_map_sharp.mrc (#32) coordinates: |
| 17269 | | Matrix rotation and translation |
| 17270 | | 0.98647244 0.16384860 0.00507628 -21.42824077 |
| 17271 | | 0.16385811 -0.98468453 -0.05955579 259.77996198 |
| 17272 | | -0.00475960 0.05958193 -0.99821207 257.16545694 |
| 17273 | | Axis 0.99660933 0.08227896 0.00007951 |
| 17274 | | Axis point 0.00000000 126.91521003 132.48208787 |
| 17275 | | Rotation angle (degrees) 176.57330142 |
| 17276 | | Shift along axis 0.03928788 |
| 17277 | | |
| 17278 | | |
| 17279 | | > select subtract #38 |
| 17280 | | |
| 17281 | | Nothing selected |
| 17282 | | |
| 17283 | | > select add #38 |
| 17284 | | |
| 17285 | | 2 models selected |
| 17286 | | |
| 17287 | | > view matrix models |
| 17288 | | > #38,-0.55814,-0.81354,-0.1632,351.46,-0.79796,0.58019,-0.16319,187.27,0.22745,0.039147,-0.973,224.57 |
| 17289 | | |
| 17290 | | > ui mousemode right "translate selected models" |
| 17291 | | |
| 17292 | | > view matrix models |
| 17293 | | > #38,-0.55814,-0.81354,-0.1632,337.55,-0.79796,0.58019,-0.16319,184.57,0.22745,0.039147,-0.973,224.6 |
| 17294 | | |
| 17295 | | > fitmap #38 inMap #32 |
| 17296 | | |
| 17297 | | Fit map cryosparc_P4_J282_005_volume_map_sharp.mrc z flip in map |
| 17298 | | cryosparc_P4_J282_005_volume_map_sharp.mrc using 208382 points |
| 17299 | | correlation = 0.4208, correlation about mean = 0.04628, overlap = 461.9 |
| 17300 | | steps = 76, shift = 2.33, angle = 4.27 degrees |
| 17301 | | |
| 17302 | | Position of cryosparc_P4_J282_005_volume_map_sharp.mrc z flip (#38) relative |
| 17303 | | to cryosparc_P4_J282_005_volume_map_sharp.mrc (#32) coordinates: |
| 17304 | | Matrix rotation and translation |
| 17305 | | -0.49732082 -0.85447621 -0.15014131 334.86076124 |
| 17306 | | -0.83743504 0.51801846 -0.17423960 201.03033487 |
| 17307 | | 0.22665956 0.03908062 -0.97318968 225.34606158 |
| 17308 | | Axis 0.49228130 -0.86954734 0.03932608 |
| 17309 | | Axis point 210.12674218 0.00000000 138.36301119 |
| 17310 | | Rotation angle (degrees) 167.48677512 |
| 17311 | | Shift along axis -1.09772430 |
| 17312 | | |
| 17313 | | |
| 17314 | | > select subtract #38 |
| 17315 | | |
| 17316 | | Nothing selected |
| 17317 | | |
| 17318 | | > select add #38 |
| 17319 | | |
| 17320 | | 2 models selected |
| 17321 | | |
| 17322 | | > view matrix models |
| 17323 | | > #38,-0.49732,-0.85448,-0.15014,335.05,-0.83744,0.51802,-0.17424,201.05,0.22666,0.039081,-0.97319,225.03 |
| 17324 | | |
| 17325 | | > ui mousemode right "rotate selected models" |
| 17326 | | |
| 17327 | | > view matrix models |
| 17328 | | > #38,-0.016367,0.99633,0.084023,-21.562,0.99914,0.013095,0.039355,-10.502,0.03811,0.084595,-0.99569,247.71 |
| 17329 | | |
| 17330 | | > ui mousemode right "translate selected models" |
| 17331 | | |
| 17332 | | > view matrix models |
| 17333 | | > #38,-0.016367,0.99633,0.084023,-11.414,0.99914,0.013095,0.039355,-8.4507,0.03811,0.084595,-0.99569,248.91 |
| 17334 | | |
| 17335 | | > fitmap #38 inMap #32 |
| 17336 | | |
| 17337 | | Fit map cryosparc_P4_J282_005_volume_map_sharp.mrc z flip in map |
| 17338 | | cryosparc_P4_J282_005_volume_map_sharp.mrc using 208382 points |
| 17339 | | correlation = 0.4638, correlation about mean = 0.04825, overlap = 549.2 |
| 17340 | | steps = 212, shift = 3.48, angle = 14 degrees |
| 17341 | | |
| 17342 | | Position of cryosparc_P4_J282_005_volume_map_sharp.mrc z flip (#38) relative |
| 17343 | | to cryosparc_P4_J282_005_volume_map_sharp.mrc (#32) coordinates: |
| 17344 | | Matrix rotation and translation |
| 17345 | | -0.11733678 0.98529275 -0.12421864 36.78201449 |
| 17346 | | 0.98526620 0.13117004 0.10974943 -32.51895072 |
| 17347 | | 0.12442908 -0.10951079 -0.98616672 260.89985464 |
| 17348 | | Axis -0.66139292 -0.75003960 -0.00008010 |
| 17349 | | Axis point 18.21387939 0.00000000 132.49954907 |
| 17350 | | Rotation angle (degrees) 170.45882216 |
| 17351 | | Shift along axis 0.04223943 |
| 17352 | | |
| 17353 | | |
| 17354 | | > select subtract #38 |
| 17355 | | |
| 17356 | | Nothing selected |
| 17357 | | |
| 17358 | | > hide #!32 models |
| 17359 | | |
| 17360 | | > color sample #38.1 map #34 palette |
| 17361 | | > 2,#ffb17f:3,#527dea:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 17362 | | |
| 17363 | | Map values for surface "surface": minimum 3.043, mean 3.992, maximum 41.6 |
| 17364 | | |
| 17365 | | > show #!34 models |
| 17366 | | |
| 17367 | | > volume flip #34 |
| 17368 | | |
| 17369 | | Opened cryosparc_P4_J287_map_locres.mrc z flip as #39, grid size 320,320,320, |
| 17370 | | pixel 0.831, shown at step 1, values float32 |
| 17371 | | |
| 17372 | | > select add #39 |
| 17373 | | |
| 17374 | | 2 models selected |
| 17375 | | |
| 17376 | | > ui mousemode right "rotate selected models" |
| 17377 | | |
| 17378 | | > view matrix models |
| 17379 | | > #39,0.92903,0.36038,-0.083822,-29.473,0.36808,-0.92323,0.1103,201.34,-0.037639,-0.13332,-0.99036,275.09 |
| 17380 | | |
| 17381 | | > show #!34 models |
| 17382 | | |
| 17383 | | > fitmap #39 imapt #34 |
| 17384 | | |
| 17385 | | Expected a keyword |
| 17386 | | |
| 17387 | | > fitmap #39 inMap #34 |
| 17388 | | |
| 17389 | | Fit map cryosparc_P4_J287_map_locres.mrc z flip in map |
| 17390 | | cryosparc_P4_J287_map_locres.mrc using 2056381 points |
| 17391 | | correlation = 0.8371, correlation about mean = 0.04941, overlap = 1.057e+08 |
| 17392 | | steps = 612, shift = 15.2, angle = 38.8 degrees |
| 17393 | | |
| 17394 | | Position of cryosparc_P4_J287_map_locres.mrc z flip (#39) relative to |
| 17395 | | cryosparc_P4_J287_map_locres.mrc (#34) coordinates: |
| 17396 | | Matrix rotation and translation |
| 17397 | | 0.49975558 0.86379414 -0.06406286 -38.69544656 |
| 17398 | | 0.86380943 -0.49157793 0.11038297 66.86486459 |
| 17399 | | 0.06385627 -0.11050260 -0.99182234 270.06743869 |
| 17400 | | Axis -0.86536171 -0.50114779 0.00005993 |
| 17401 | | Axis point 0.00000000 54.63554700 132.55703766 |
| 17402 | | Rotation angle (degrees) 172.66756182 |
| 17403 | | Shift along axis -0.00743630 |
| 17404 | | |
| 17405 | | |
| 17406 | | > select subtract #39 |
| 17407 | | |
| 17408 | | Nothing selected |
| 17409 | | |
| 17410 | | > hide #!39 models |
| 17411 | | |
| 17412 | | > show #!39 models |
| 17413 | | |
| 17414 | | > select add #39 |
| 17415 | | |
| 17416 | | 2 models selected |
| 17417 | | |
| 17418 | | > hide #37 models |
| 17419 | | |
| 17420 | | > hide #!38 models |
| 17421 | | |
| 17422 | | > view matrix models |
| 17423 | | > #39,0.37237,-0.92808,0.0029165,218.26,-0.92659,-0.37195,-0.055474,318.12,0.052569,0.017955,-0.99846,254.69 |
| 17424 | | |
| 17425 | | > ui mousemode right "translate selected models" |
| 17426 | | |
| 17427 | | > view matrix models |
| 17428 | | > #39,0.37237,-0.92808,0.0029165,208.8,-0.92659,-0.37195,-0.055474,313.9,0.052569,0.017955,-0.99846,255.36 |
| 17429 | | |
| 17430 | | > ui mousemode right "rotate selected models" |
| 17431 | | |
| 17432 | | > view matrix models |
| 17433 | | > #39,0.66947,-0.74091,0.05349,135.56,-0.73453,-0.67101,-0.10103,334.54,0.11074,0.028346,-0.99344,245.27 |
| 17434 | | |
| 17435 | | > ui mousemode right "translate selected models" |
| 17436 | | |
| 17437 | | > view matrix models |
| 17438 | | > #39,0.66947,-0.74091,0.05349,211.58,-0.73453,-0.67101,-0.10103,368.3,0.11074,0.028346,-0.99344,245.76 |
| 17439 | | |
| 17440 | | > view matrix models |
| 17441 | | > #39,0.66947,-0.74091,0.05349,241.5,-0.73453,-0.67101,-0.10103,381.69,0.11074,0.028346,-0.99344,243.08 |
| 17442 | | |
| 17443 | | > view matrix models |
| 17444 | | > #39,0.66947,-0.74091,0.05349,287.43,-0.73453,-0.67101,-0.10103,402.31,0.11074,0.028346,-0.99344,237.25 |
| 17445 | | |
| 17446 | | > ui mousemode right "rotate selected models" |
| 17447 | | |
| 17448 | | > view matrix models |
| 17449 | | > #39,-0.41494,0.89077,-0.18535,241.42,0.8738,0.44692,0.19168,-11.043,0.25358,-0.082424,-0.9638,229.13 |
| 17450 | | |
| 17451 | | > view matrix models |
| 17452 | | > #39,0.99948,-0.019992,0.025148,145.79,-0.021975,-0.99645,0.081215,326.06,0.023435,-0.081726,-0.99638,264.88 |
| 17453 | | |
| 17454 | | > ui mousemode right "translate selected models" |
| 17455 | | |
| 17456 | | > view matrix models |
| 17457 | | > #39,0.99948,-0.019992,0.025148,-4.0949,-0.021975,-0.99645,0.081215,259.26,0.023435,-0.081726,-0.99638,270.42 |
| 17458 | | |
| 17459 | | > select subtract #39 |
| 17460 | | |
| 17461 | | Nothing selected |
| 17462 | | |
| 17463 | | > hide #!34 models |
| 17464 | | |
| 17465 | | > color sample #38.1 map #34 palette |
| 17466 | | > 2,#ffb17f:3,#527dea:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 17467 | | |
| 17468 | | Map values for surface "surface": minimum 3.043, mean 3.992, maximum 41.6 |
| 17469 | | |
| 17470 | | > hide #!39 models |
| 17471 | | |
| 17472 | | > show #!38 models |
| 17473 | | |
| 17474 | | > open "/Users/peiyuyu/Documents/Goethe |
| 17475 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 17476 | | > F_B1/cryosparc_P4_J282_005_volume_map_sharp.mrc" |
| 17477 | | |
| 17478 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc as #40, grid size |
| 17479 | | 320,320,320, pixel 0.831, shown at level 0.0392, step 2, values float32 |
| 17480 | | |
| 17481 | | > hide #!38 models |
| 17482 | | |
| 17483 | | > open "/Users/peiyuyu/Documents/Goethe |
| 17484 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 17485 | | > F_B1/cryosparc_P4_J287_map_locres.mrc" |
| 17486 | | |
| 17487 | | Opened cryosparc_P4_J287_map_locres.mrc as #41, grid size 320,320,320, pixel |
| 17488 | | 0.831, shown at level 9.77, step 2, values float32 |
| 17489 | | |
| 17490 | | > volume flip #40 |
| 17491 | | |
| 17492 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc z flip as #42, grid size |
| 17493 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 17494 | | |
| 17495 | | > volume flip #41 |
| 17496 | | |
| 17497 | | Opened cryosparc_P4_J287_map_locres.mrc z flip as #43, grid size 320,320,320, |
| 17498 | | pixel 0.831, shown at step 1, values float32 |
| 17499 | | |
| 17500 | | > volume #43 level 4.042 |
| 17501 | | |
| 17502 | | > color sample #42.1 map #43 palette |
| 17503 | | > 2,#ffb17f:3,#527dea:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 17504 | | |
| 17505 | | Map values for surface "surface": minimum 0, mean 4.484, maximum 42.57 |
| 17506 | | |
| 17507 | | > hide #!43 models |
| 17508 | | |
| 17509 | | > hide #!42 models |
| 17510 | | |
| 17511 | | > show #!38 models |
| 17512 | | |
| 17513 | | > show #!39 models |
| 17514 | | |
| 17515 | | > show #!40 models |
| 17516 | | |
| 17517 | | > show #!41 models |
| 17518 | | |
| 17519 | | > show #!42 models |
| 17520 | | |
| 17521 | | > show #!43 models |
| 17522 | | |
| 17523 | | > close #43 |
| 17524 | | |
| 17525 | | > close #38 |
| 17526 | | |
| 17527 | | > close #42 |
| 17528 | | |
| 17529 | | > close #41 |
| 17530 | | |
| 17531 | | > close #40 |
| 17532 | | |
| 17533 | | > close #39 |
| 17534 | | |
| 17535 | | > close #37 |
| 17536 | | |
| 17537 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 17538 | | |
| 17539 | | > ui mousemode right "color key" |
| 17540 | | |
| 17541 | | > hide #36 models |
| 17542 | | |
| 17543 | | > volume flip #32 |
| 17544 | | |
| 17545 | | Opened cryosparc_P4_J282_005_volume_map_sharp.mrc z flip as #37, grid size |
| 17546 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 17547 | | |
| 17548 | | > volume flip #34 |
| 17549 | | |
| 17550 | | Opened cryosparc_P4_J287_map_locres.mrc z flip as #38, grid size 320,320,320, |
| 17551 | | pixel 0.831, shown at step 1, values float32 |
| 17552 | | |
| 17553 | | > volume flip #33 |
| 17554 | | |
| 17555 | | Opened cryosparc_P4_J285_006_volume_map_sharp.mrc z flip as #39, grid size |
| 17556 | | 320,320,320, pixel 0.831, shown at step 1, values float32 |
| 17557 | | |
| 17558 | | > volume flip #35 |
| 17559 | | |
| 17560 | | Opened cryosparc_P4_J288_map_locres.mrc z flip as #40, grid size 320,320,320, |
| 17561 | | pixel 0.831, shown at step 1, values float32 |
| 17562 | | |
| 17563 | | > hide #!39 models |
| 17564 | | |
| 17565 | | > hide #!40 models |
| 17566 | | |
| 17567 | | > hide #!38 models |
| 17568 | | |
| 17569 | | > color sample #37.1 map #38 palette |
| 17570 | | > 2,#ffb17f:3,#527dea:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 17571 | | |
| 17572 | | Map values for surface "surface": minimum 0, mean 3.731, maximum 41.43 |
| 17573 | | |
| 17574 | | > hide #!37 models |
| 17575 | | |
| 17576 | | > show #!37 models |
| 17577 | | |
| 17578 | | > hide #!37 models |
| 17579 | | |
| 17580 | | > show #!39 models |
| 17581 | | |
| 17582 | | > color sample #39.1 map #40 palette |
| 17583 | | > 2,#ffb17f:3,#527dea:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080 |
| 17584 | | |
| 17585 | | Map values for surface "surface": minimum 0, mean 3.67, maximum 40.73 |
| 17586 | | |
| 17587 | | > volume #39 level 0.05082 |
| 17588 | | |
| 17589 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 17590 | | |
| 17591 | | > show #36 models |
| 17592 | | |
| 17593 | | > select add #36 |
| 17594 | | |
| 17595 | | 1 model selected |
| 17596 | | |
| 17597 | | > key pos 0.366468,0.16715 |
| 17598 | | |
| 17599 | | > key pos 0.360149,0.168599 |
| 17600 | | |
| 17601 | | > key labelColor #bfbfbf |
| 17602 | | |
| 17603 | | > key labelColor default |
| 17604 | | |
| 17605 | | > key labelColor #bfbfbf |
| 17606 | | |
| 17607 | | > key labelColor white |
| 17608 | | |
| 17609 | | > key labelColor default |
| 17610 | | |
| 17611 | | > key ticks true |
| 17612 | | |
| 17613 | | > key tickLength 8.0 |
| 17614 | | |
| 17615 | | > key tickThickness 3.0 |
| 17616 | | |
| 17617 | | > key tickThickness 2.0 |
| 17618 | | |
| 17619 | | > key tickThickness 1.0 |
| 17620 | | |
| 17621 | | > key tickLength 10.0 |
| 17622 | | |
| 17623 | | > key tickLength 8.0 |
| 17624 | | |
| 17625 | | > key tickThickness 2.0 |
| 17626 | | |
| 17627 | | > key tickThickness 1.0 |
| 17628 | | |
| 17629 | | > key tickThickness 0.0 |
| 17630 | | |
| 17631 | | > key tickThickness 1.0 |
| 17632 | | |
| 17633 | | > key tickThickness 2.0 |
| 17634 | | |
| 17635 | | > key ticks false |
| 17636 | | |
| 17637 | | > key labelOffset -1.0 |
| 17638 | | |
| 17639 | | > key labelOffset -2.0 |
| 17640 | | |
| 17641 | | > key labelOffset -1.0 |
| 17642 | | |
| 17643 | | > key labelOffset 0.0 |
| 17644 | | |
| 17645 | | > key labelOffset 1.0 |
| 17646 | | |
| 17647 | | > key labelOffset 2.0 |
| 17648 | | |
| 17649 | | > key labelOffset 3.0 |
| 17650 | | |
| 17651 | | > key labelOffset 4.0 |
| 17652 | | |
| 17653 | | > view matrix models #36,1,0,0,-0.86321,0,1,0,-0.41592,0,0,1,0.9887 |
| 17654 | | |
| 17655 | | > select subtract #36 |
| 17656 | | |
| 17657 | | Nothing selected |
| 17658 | | |
| 17659 | | > select add #36 |
| 17660 | | |
| 17661 | | 1 model selected |
| 17662 | | |
| 17663 | | > view matrix models #36,1,0,0,-99.598,0,1,0,-41.849,0,0,1,63.569 |
| 17664 | | |
| 17665 | | > view matrix models #36,1,0,0,-99.715,0,1,0,-41.895,0,0,1,63.621 |
| 17666 | | |
| 17667 | | > view matrix models #36,1,0,0,-102.61,0,1,0,-42.915,0,0,1,63.831 |
| 17668 | | |
| 17669 | | > view matrix models #36,1,0,0,-126.49,0,1,0,-56.195,0,0,1,105.79 |
| 17670 | | |
| 17671 | | > view matrix models #36,1,0,0,-152.42,0,1,0,-56.013,0,0,1,31.21 |
| 17672 | | |
| 17673 | | > view matrix models #36,1,0,0,-131.29,0,1,0,-47.985,0,0,1,24.738 |
| 17674 | | |
| 17675 | | > view matrix models #36,1,0,0,-121.36,0,1,0,-44.649,0,0,1,25.261 |
| 17676 | | |
| 17677 | | > view matrix models #36,1,0,0,-121.37,0,1,0,-44.69,0,0,1,25.567 |
| 17678 | | |
| 17679 | | > view matrix models #36,1,0,0,-123.18,0,1,0,-45.336,0,0,1,25.776 |
| 17680 | | |
| 17681 | | > close #36 |
| 17682 | | |
| 17683 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 17684 | | |
| 17685 | | > ui mousemode right "color key" |
| 17686 | | |
| 17687 | | > key pos 0.377773,0.195303 |
| 17688 | | |
| 17689 | | > key pos 0.377554,0.185959 |
| 17690 | | |
| 17691 | | > key borderWidth 3.0 |
| 17692 | | |
| 17693 | | > key labelOffset 1.0 |
| 17694 | | |
| 17695 | | > key labelOffset 2.0 |
| 17696 | | |
| 17697 | | > key labelOffset 3.0 |
| 17698 | | |
| 17699 | | > key labelOffset 4.0 |
| 17700 | | |
| 17701 | | > key labelOffset 5.0 |
| 17702 | | |
| 17703 | | > key labelOffset 6.0 |
| 17704 | | |
| 17705 | | > key size 0.24000,0.05000 |
| 17706 | | |
| 17707 | | > key size 0.23000,0.05000 |
| 17708 | | |
| 17709 | | > key size 0.23000,0.04000 |
| 17710 | | |
| 17711 | | > key size 0.23000,0.03000 |
| 17712 | | |
| 17713 | | > key size 0.22000,0.03000 |
| 17714 | | |
| 17715 | | > key size 0.21000,0.03000 |
| 17716 | | |
| 17717 | | > key size 0.20000,0.03000 |
| 17718 | | |
| 17719 | | > key size 0.19000,0.03000 |
| 17720 | | |
| 17721 | | > key size 0.18000,0.03000 |
| 17722 | | |
| 17723 | | > key size 0.17000,0.03000 |
| 17724 | | |
| 17725 | | > key size 0.16000,0.03000 |
| 17726 | | |
| 17727 | | > key size 0.15000,0.03000 |
| 17728 | | |
| 17729 | | > key size 0.16000,0.03000 |
| 17730 | | |
| 17731 | | > key size 0.17000,0.03000 |
| 17732 | | |
| 17733 | | > key size 0.17000,0.04000 |
| 17734 | | |
| 17735 | | > key size 0.17000,0.05000 |
| 17736 | | |
| 17737 | | > key size 0.17000,0.04000 |
| 17738 | | |
| 17739 | | > key pos 0.36134,0.205524 size 0.0323267,0.199875 |
| 17740 | | |
| 17741 | | > key pos 0.375585,0.243532 size 0.000780577,0.00120384 |
| 17742 | | |
| 17743 | | > key pos 0.393081,0.282969 size 0,0 |
| 17744 | | |
| 17745 | | [Repeated 1 time(s)] |
| 17746 | | |
| 17747 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 17748 | | |
| 17749 | | > hide #!39 models |
| 17750 | | |
| 17751 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 17752 | | |
| 17753 | | > ui mousemode right "color key" |
| 17754 | | |
| 17755 | | > key pos 0.654167,0.35194 size 0.0389145,0.208023 |
| 17756 | | |
| 17757 | | > show #!39 models |
| 17758 | | |
| 17759 | | > key pos 0.434243,0.168612 size 0.0379186,0.161115 |
| 17760 | | |
| 17761 | | > hide #41 models |
| 17762 | | |
| 17763 | | > ui mousemode right "translate selected models" |
| 17764 | | |
| 17765 | | > ui mousemode right translate |
| 17766 | | |
| 17767 | | [Repeated 1 time(s)] |
| 17768 | | |
| 17769 | | > select add #41 |
| 17770 | | |
| 17771 | | 1 model selected |
| 17772 | | |
| 17773 | | > select subtract #41 |
| 17774 | | |
| 17775 | | Nothing selected |
| 17776 | | |
| 17777 | | > show #41 models |
| 17778 | | |
| 17779 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 17780 | | |
| 17781 | | > ui mousemode right select |
| 17782 | | |
| 17783 | | > select clear |
| 17784 | | |
| 17785 | | > ui mousemode right rotate |
| 17786 | | |
| 17787 | | > ui mousemode right translate |
| 17788 | | |
| 17789 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 17790 | | |
| 17791 | | [Repeated 1 time(s)] |
| 17792 | | |
| 17793 | | > ui mousemode right zoom |
| 17794 | | |
| 17795 | | > ui mousemode right "translate selected models" |
| 17796 | | |
| 17797 | | > ui mousemode right select |
| 17798 | | |
| 17799 | | > select clear |
| 17800 | | |
| 17801 | | > select add #41 |
| 17802 | | |
| 17803 | | 1 model selected |
| 17804 | | |
| 17805 | | > select clear |
| 17806 | | |
| 17807 | | Drag select of 39 cryosparc_P4_J285_006_volume_map_sharp.mrc z flip |
| 17808 | | |
| 17809 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 17810 | | |
| 17811 | | > select clear |
| 17812 | | |
| 17813 | | Drag select of 39 cryosparc_P4_J285_006_volume_map_sharp.mrc z flip |
| 17814 | | |
| 17815 | | > ui mousemode right translate |
| 17816 | | |
| 17817 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 17818 | | |
| 17819 | | > select subtract #41 |
| 17820 | | |
| 17821 | | 2 models selected |
| 17822 | | |
| 17823 | | > ui mousemode right "translate selected models" |
| 17824 | | |
| 17825 | | > ui mousemode right rotate |
| 17826 | | |
| 17827 | | > ui mousemode right translate |
| 17828 | | |
| 17829 | | > hide #!39 models |
| 17830 | | |
| 17831 | | > show #!39 models |
| 17832 | | |
| 17833 | | > ui mousemode right select |
| 17834 | | |
| 17835 | | > select #39 |
| 17836 | | |
| 17837 | | 2 models selected |
| 17838 | | |
| 17839 | | > ui mousemode right rotate |
| 17840 | | |
| 17841 | | > ui mousemode right translate |
| 17842 | | |
| 17843 | | > select subtract #39 |
| 17844 | | |
| 17845 | | Nothing selected |
| 17846 | | |
| 17847 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 17848 | | |
| 17849 | | > hide #!39 models |
| 17850 | | |
| 17851 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 17852 | | |
| 17853 | | > ui mousemode left "color key" |
| 17854 | | |
| 17855 | | > ui mousemode left rotate |
| 17856 | | |
| 17857 | | > ui mousemode right 'color key' |
| 17858 | | |
| 17859 | | > key pos 0.683937,0.290603 size 0.0366198,0.178803 |
| 17860 | | |
| 17861 | | > show #!39 models |
| 17862 | | |
| 17863 | | > key pos 0.885363,0.663755 size 0.0185522,0.00687018 |
| 17864 | | |
| 17865 | | > ui mousemode right translate |
| 17866 | | |
| 17867 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 17868 | | |
| 17869 | | > ui mousemode right "color key" |
| 17870 | | |
| 17871 | | > key pos 0.768821,0.24433 size 0.0347996,0.266198 |
| 17872 | | |
| 17873 | | > key labelOffset 7.0 |
| 17874 | | |
| 17875 | | > key labelOffset 8.0 |
| 17876 | | |
| 17877 | | > key labelOffset 9.0 |
| 17878 | | |
| 17879 | | > hide #!39 models |
| 17880 | | |
| 17881 | | > show #!37 models |
| 17882 | | |
| 17883 | | > volume #37 level 0.06504 |
| 17884 | | |
| 17885 | | > save "/Users/peiyuyu/Documents/Goethe |
| 17886 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 17887 | | > F_B1/20260503_SA-F_B1_workflow2.cxs" |
| 17888 | | |
| 17889 | | > key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true |
| 17890 | | |
| 17891 | | > ui mousemode right "color key" |
| 17892 | | |
| 17893 | | > key fontSize 25 |
| 17894 | | |
| 17895 | | > key fontSize 26 |
| 17896 | | |
| 17897 | | > key fontSize 27 |
| 17898 | | |
| 17899 | | > key fontSize 28 |
| 17900 | | |
| 17901 | | > key fontSize 29 |
| 17902 | | |
| 17903 | | > key fontSize 30 |
| 17904 | | |
| 17905 | | > key fontSize 31 |
| 17906 | | |
| 17907 | | > key fontSize 32 |
| 17908 | | |
| 17909 | | > key fontSize 33 |
| 17910 | | |
| 17911 | | > key fontSize 34 |
| 17912 | | |
| 17913 | | > key fontSize 35 |
| 17914 | | |
| 17915 | | > save CS_P4_J282_locres_sharp.png supersample 3 transparentBackground true |
| 17916 | | |
| 17917 | | > hide #!37 models |
| 17918 | | |
| 17919 | | > show #!39 models |
| 17920 | | |
| 17921 | | > volume #39 level 0.06897 |
| 17922 | | |
| 17923 | | > volume #39 level 0.06638 |
| 17924 | | |
| 17925 | | > save CS_P4_J285_locres_sharp.png supersample 3 transparentBackground true |
| 17926 | | |
| 17927 | | > save "/Users/peiyuyu/Documents/Goethe |
| 17928 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 17929 | | > F_B1/20260503_SA-F_B1_workflow2.cxs" |
| 17930 | | |
| 17931 | | > hide #!39 models |
| 17932 | | |
| 17933 | | > hide #41 models |
| 17934 | | |
| 17935 | | > open "/Users/peiyuyu/Documents/Goethe |
| 17936 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 17937 | | > F_B1/CS_P4_J282_sharp_flip.mrc" |
| 17938 | | |
| 17939 | | Opened CS_P4_J282_sharp_flip.mrc as #42, grid size 320,320,320, pixel 0.831, |
| 17940 | | shown at level 0.0392, step 2, values float32 |
| 17941 | | |
| 17942 | | > open "/Users/peiyuyu/Documents/Goethe University/19. Model Building/2. AcrF |
| 17943 | | > Project/2. Bound-form/SA-F_B1/Model/aligned_model_for_J282.pdb" |
| 17944 | | |
| 17945 | | Chain information for aligned_model_for_J282.pdb #43 |
| 17946 | | --- |
| 17947 | | Chain | Description |
| 17948 | | A B C | No description available |
| 17949 | | |
| 17950 | | |
| 17951 | | > hide #!42 models |
| 17952 | | |
| 17953 | | > select #43/BC |
| 17954 | | |
| 17955 | | Nothing selected |
| 17956 | | |
| 17957 | | > select #43/B, C |
| 17958 | | |
| 17959 | | 15672 atoms, 15966 bonds, 2068 residues, 1 model selected |
| 17960 | | |
| 17961 | | > delete sel |
| 17962 | | |
| 17963 | | > show #!42 models |
| 17964 | | |
| 17965 | | > volume zone #42 nearAtoms #43/A |
| 17966 | | |
| 17967 | | > color #43 #FFF6C0 |
| 17968 | | |
| 17969 | | > volume zone #42 nearAtoms #43/A distance 5 |
| 17970 | | |
| 17971 | | Expected a keyword |
| 17972 | | |
| 17973 | | > color zone #42 near #43/A distance 5 |
| 17974 | | |
| 17975 | | > color zone #42 near #43/A distance 5 tran 50 |
| 17976 | | |
| 17977 | | Expected a keyword |
| 17978 | | |
| 17979 | | > color zone #42 near #43/A distance 5 tran 50 |
| 17980 | | |
| 17981 | | Expected a keyword |
| 17982 | | |
| 17983 | | > color #43 #FFF6C0 |
| 17984 | | |
| 17985 | | > color zone #42 near #43/A distance 5 |
| 17986 | | |
| 17987 | | > color #43 #FFF6C0 transparency 50 |
| 17988 | | |
| 17989 | | > color zone #42 near #43/A distance 5 |
| 17990 | | |
| 17991 | | > graphics silhouettes false |
| 17992 | | |
| 17993 | | > lighting full |
| 17994 | | |
| 17995 | | > lighting flat |
| 17996 | | |
| 17997 | | [Repeated 1 time(s)] |
| 17998 | | |
| 17999 | | > lighting shadows true intensity 0.5 |
| 18000 | | |
| 18001 | | > lighting shadows false |
| 18002 | | |
| 18003 | | > lighting soft |
| 18004 | | |
| 18005 | | [Repeated 1 time(s)] |
| 18006 | | |
| 18007 | | > lighting simple |
| 18008 | | |
| 18009 | | > graphics silhouettes false |
| 18010 | | |
| 18011 | | > color #42 #99bfe580 models |
| 18012 | | |
| 18013 | | > hide #43 models |
| 18014 | | |
| 18015 | | > transparency #42 50 |
| 18016 | | |
| 18017 | | > lighting soft |
| 18018 | | |
| 18019 | | > volume #42 level 0.0232 |
| 18020 | | |
| 18021 | | > lighting soft |
| 18022 | | |
| 18023 | | > close #42 |
| 18024 | | |
| 18025 | | > close #43 |
| 18026 | | |
| 18027 | | > open "/Users/peiyuyu/Documents/Goethe |
| 18028 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 18029 | | > F_B1/cryosparc_P4_J242_class_02_00122_volume_sharp.mrc" |
| 18030 | | |
| 18031 | | Opened cryosparc_P4_J242_class_02_00122_volume_sharp.mrc as #36, grid size |
| 18032 | | 128,128,128, pixel 2.08, shown at level 0.195, step 1, values float32 |
| 18033 | | |
| 18034 | | > open "/Users/peiyuyu/Documents/Goethe |
| 18035 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 18036 | | > F_B1/cryosparc_P4_J242_class_01_00122_volume_sharp.mrc" |
| 18037 | | |
| 18038 | | Opened cryosparc_P4_J242_class_01_00122_volume_sharp.mrc as #42, grid size |
| 18039 | | 128,128,128, pixel 2.08, shown at level 0.178, step 1, values float32 |
| 18040 | | |
| 18041 | | > close #36 |
| 18042 | | |
| 18043 | | > show #!9 models |
| 18044 | | |
| 18045 | | > select add #42 |
| 18046 | | |
| 18047 | | 2 models selected |
| 18048 | | |
| 18049 | | > ui mousemode right "rotate selected models" |
| 18050 | | |
| 18051 | | > view matrix models |
| 18052 | | > #42,0.026241,-0.92593,0.37678,198.52,-0.063177,0.37462,0.92502,-34.721,-0.99766,-0.048078,-0.048667,279.22 |
| 18053 | | |
| 18054 | | > ui mousemode right "translate selected models" |
| 18055 | | |
| 18056 | | > view matrix models |
| 18057 | | > #42,0.026241,-0.92593,0.37678,198.7,-0.063177,0.37462,0.92502,-33.267,-0.99766,-0.048078,-0.048667,263 |
| 18058 | | |
| 18059 | | > fitmap #42 inMap #9 |
| 18060 | | |
| 18061 | | Fit map cryosparc_P4_J242_class_01_00122_volume_sharp.mrc in map |
| 18062 | | cryosparc_P4_J242_class_01_00122_volume_sharp.mrc using 20960 points |
| 18063 | | correlation = 1, correlation about mean = 1, overlap = 1571 |
| 18064 | | steps = 128, shift = 5.08, angle = 27.9 degrees |
| 18065 | | |
| 18066 | | Position of cryosparc_P4_J242_class_01_00122_volume_sharp.mrc (#42) relative |
| 18067 | | to cryosparc_P4_J242_class_01_00122_volume_sharp.mrc (#9) coordinates: |
| 18068 | | Matrix rotation and translation |
| 18069 | | 0.99999999 -0.00010909 0.00000609 0.02020954 |
| 18070 | | 0.00010909 0.99999995 0.00031305 -0.06272325 |
| 18071 | | -0.00000612 -0.00031305 0.99999995 0.03217050 |
| 18072 | | Axis -0.94414385 0.01842055 0.32901836 |
| 18073 | | Axis point 0.00000000 112.87704178 199.81299213 |
| 18074 | | Rotation angle (degrees) 0.01899762 |
| 18075 | | Shift along axis -0.00965143 |
| 18076 | | |
| 18077 | | |
| 18078 | | > select subtract #42 |
| 18079 | | |
| 18080 | | Nothing selected |
| 18081 | | |
| 18082 | | > select add #42 |
| 18083 | | |
| 18084 | | 2 models selected |
| 18085 | | |
| 18086 | | > fitmap #42 inMap #9 |
| 18087 | | |
| 18088 | | Fit map cryosparc_P4_J242_class_01_00122_volume_sharp.mrc in map |
| 18089 | | cryosparc_P4_J242_class_01_00122_volume_sharp.mrc using 20960 points |
| 18090 | | correlation = 1, correlation about mean = 1, overlap = 1571 |
| 18091 | | steps = 40, shift = 0.00318, angle = 0.00769 degrees |
| 18092 | | |
| 18093 | | Position of cryosparc_P4_J242_class_01_00122_volume_sharp.mrc (#42) relative |
| 18094 | | to cryosparc_P4_J242_class_01_00122_volume_sharp.mrc (#9) coordinates: |
| 18095 | | Matrix rotation and translation |
| 18096 | | 0.99999999 -0.00011772 0.00012933 0.00283694 |
| 18097 | | 0.00011769 0.99999996 0.00026066 -0.05912201 |
| 18098 | | -0.00012937 -0.00026064 0.99999996 0.04294746 |
| 18099 | | Axis -0.83040251 0.41209275 0.37498166 |
| 18100 | | Axis point 0.00000000 180.01203882 210.06369623 |
| 18101 | | Rotation angle (degrees) 0.01798432 |
| 18102 | | Shift along axis -0.01061505 |
| 18103 | | |
| 18104 | | |
| 18105 | | > select subtract #42 |
| 18106 | | |
| 18107 | | Nothing selected |
| 18108 | | |
| 18109 | | > hide #!9 models |
| 18110 | | |
| 18111 | | > volume #42 level 0.2579 |
| 18112 | | |
| 18113 | | > color #42 #ffffb2ff models |
| 18114 | | |
| 18115 | | > color #42 #ffffb280 models |
| 18116 | | |
| 18117 | | > open "/Users/peiyuyu/Documents/Goethe |
| 18118 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 18119 | | > F_B1/CS_P4_J282_sharp_flip.mrc" |
| 18120 | | |
| 18121 | | Opened CS_P4_J282_sharp_flip.mrc as #36, grid size 320,320,320, pixel 0.831, |
| 18122 | | shown at level 0.0392, step 2, values float32 |
| 18123 | | |
| 18124 | | > volume #42 level 0.1954 |
| 18125 | | |
| 18126 | | > select add #42 |
| 18127 | | |
| 18128 | | 2 models selected |
| 18129 | | |
| 18130 | | > view matrix models |
| 18131 | | > #42,0.15189,-0.98482,-0.084022,248.42,-0.056394,-0.093504,0.99402,24.018,-0.98679,-0.14625,-0.069741,273.86 |
| 18132 | | |
| 18133 | | > volume #42 level 0.2537 |
| 18134 | | |
| 18135 | | > open "/Users/peiyuyu/Documents/Goethe |
| 18136 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 18137 | | > F_B1/cryosparc_P4_J169_class_03_00182_volume_sharp.mrc" |
| 18138 | | |
| 18139 | | Opened cryosparc_P4_J169_class_03_00182_volume_sharp.mrc as #43, grid size |
| 18140 | | 128,128,128, pixel 2.08, shown at level 0.248, step 1, values float32 |
| 18141 | | |
| 18142 | | > close #43 |
| 18143 | | |
| 18144 | | > open "/Users/peiyuyu/Documents/Goethe |
| 18145 | | > University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA- |
| 18146 | | > F_B1/cryosparc_P4_J169_class_02_00182_volume_sharp.mrc" |
| 18147 | | |
| 18148 | | Opened cryosparc_P4_J169_class_02_00182_volume_sharp.mrc as #43, grid size |
| 18149 | | 128,128,128, pixel 2.08, shown at level 0.216, step 1, values float32 |
| 18150 | | |
| 18151 | | > select add #43 |
| 18152 | | |
| 18153 | | 4 models selected |
| 18154 | | |
| 18155 | | > view matrix models |
| 18156 | | > #42,0.15189,-0.98482,-0.084022,246.73,-0.056394,-0.093504,0.99402,-45.124,-0.98679,-0.14625,-0.069741,235.92,#43,1,0,0,-1.6882,0,1,0,-69.141,0,0,1,-37.947 |
| 18157 | | |
| 18158 | | > view matrix models |
| 18159 | | > #42,0.15189,-0.98482,-0.084022,237,-0.056394,-0.093504,0.99402,126.12,-0.98679,-0.14625,-0.069741,312.08,#43,1,0,0,-11.419,0,1,0,102.11,0,0,1,38.221 |
| 18160 | | |
| 18161 | | > select subtract #42 |
| 18162 | | |
| 18163 | | 2 models selected |
| 18164 | | |
| | 2716 | Deeleted thousands of lines to meet Trac size limit. Tom |
![[ChimeraX Issue Tracking System]](/trac/ChimeraX/chrome/site/ChimeraX-icon.svg){kind=icon}
