id summary reporter owner description type status priority milestone component version resolution keywords cc blockedby blocking notify_on_close platform project 3379 Volume tilted slab to plane: glClear invalid framebuffer operation goddard@… Tom Goddard "{{{ The following bug report has been submitted: Platform: Darwin-19.5.0-x86_64-i386-64bit ChimeraX Version: 1.1.dev202006082325 (2020-06-08 23:25:53 UTC) Description Switched from tilted slab to single plane using volume viewer menu on multichannel light microscopy from Allen Insititute. Log: UCSF ChimeraX version: 1.1.dev202006082325 (2020-06-08) © 2016-2020 Regents of the University of California. All rights reserved. How to cite UCSF ChimeraX > open /Users/goddard/ucsf/data/allen_cell/AICS-12/AICS-12_269.ome.tif Opened multi-channel map AICS-12_269.ome.tif, 8 channels, grid size 924,624,47, pixel 0.108,0.108,0.29, shown at step 2, values uint16 > volume #1 showOutlineBox true imageMode ""tilted slab"" tiltedSlabOffset 6.453 > tiltedSlabSpacing 0.1083 tiltedSlabPlaneCount 4 > volume #1.1 tiltedSlabAxis -0.08897,0.02955,0.9956 tiltedSlabOffset 1.524 > volume #1.1 tiltedSlabAxis 0.01323,-0.07022,0.9974 tiltedSlabOffset 4.38 > volume #1.1 tiltedSlabAxis 0.01323,-0.07022,0.9974 tiltedSlabOffset 2.839 > set bgColor white > ui mousemode right ""crop volume"" > volume #1.1 tiltedSlabOffset 2.839 tiltedSlabPlaneCount 11 > volume #1.1 tiltedSlabOffset 2.839 tiltedSlabPlaneCount 16 > set bgColor black > volume #1.1 tiltedSlabOffset 2.839 tiltedSlabPlaneCount 9 > volume #1 region 0,0,0,923,623,46 step 1 colorMode opaque8 orthoplanes xyz > positionPlanes 461,311,23 imageMode orthoplanes > set bgColor white > volume #1.1 level 1057,0 level 4929,0.8 level 1.96e+04,1 > volume #1.1 level 1057,0 level 3245,0.7706 level 1.96e+04,1 > volume #1.2 level 790.3,0 level 2311,0.8 level 8275,1 > volume #1.2 level 790.3,0 level 1949,0.8441 level 8275,1 > volume #1.3 level 403,0 level 485,0.8 level 2069,1 > volume #1.3 level 403,0 level 453.6,0.8147 level 2069,1 > volume #1.1 orthoplanes xyz positionPlanes 461,441,23 > volume #1.1 orthoplanes xyz positionPlanes 685,441,23 > volume #1.1 orthoplanes xyz positionPlanes 685,387,23 > select clear > volume #1 region 0,0,0,923,623,46 step 2 colorMode auto8 imageMode ""tilted > slab"" tiltedSlabAxis 0.5152,-0.7002,-0.4943 tiltedSlabOffset -1.377 > tiltedSlabPlaneCount 4 > set bgColor black > volume #1.1 tiltedSlabAxis 0.04408,0.009686,-0.999 tiltedSlabOffset 2.406 > volume #1.1 tiltedSlabAxis 0.04408,0.009686,-0.999 tiltedSlabOffset -1.391 > volume #1.1 tiltedSlabAxis 0.09182,0.05938,-0.994 tiltedSlabOffset 2.547 > volume #1.1 tiltedSlabAxis 0.09182,0.05938,-0.994 tiltedSlabOffset 0.5921 > volume #1.1 tiltedSlabAxis 0.09182,0.05938,-0.994 tiltedSlabOffset 0.01317 > volume #1.1 tiltedSlabAxis 0.0287,-0.04669,-0.9985 tiltedSlabOffset -5.7 > set bgColor white > volume #1 outlineBoxRgb black > volume #1 backingColor black > ui mousemode right ""crop volume"" > volume #1.1 tiltedSlabOffset -7.108 tiltedSlabPlaneCount 17 > ui mousemode right ""move planes"" > volume #1.1 tiltedSlabOffset -4.183 tiltedSlabPlaneCount 17 > volume #1.1 tiltedSlabOffset -4.616 tiltedSlabPlaneCount 17 > ui mousemode right ""rotate slab"" > volume #1.1 tiltedSlabAxis 0.03927,-0.02371,-0.9989 tiltedSlabOffset -3.324 > ui mousemode right ""move planes"" > volume #1.1 tiltedSlabOffset -4.299 tiltedSlabPlaneCount 17 > volume #1.1 tiltedSlabOffset -4.624 tiltedSlabPlaneCount 17 > ui mousemode right ""rotate slab"" > volume #1.1 tiltedSlabAxis -0.007982,0.005278,-1 tiltedSlabOffset -5.965 > volume #1 step 1 > volume #1.1 level 1057,0 level 3174,0.8441 level 1.96e+04,1 > volume #1.3 level 403,0 level 469.8,0.7941 level 2069,1 > ui mousemode right ""crop volume"" > volume #1.1 tiltedSlabOffset -3.473 tiltedSlabPlaneCount 14 > ui mousemode right ""move planes"" > volume #1.1 tiltedSlabOffset -5.098 tiltedSlabPlaneCount 14 An error occurred in drawing the scene. Redrawing graphics is now stopped to avoid a continuous stream of error messages. To restart graphics use the command ""graphics restart"" after changing the settings that caused the error. Traceback (most recent call last): File ""/Users/goddard/ucsf/chimerax/ChimeraX.app/Contents/Library/Frameworks/Python.framework/Versions/3.7/lib/python3.7/site- packages/chimerax/core/updateloop.py"", line 73, in draw_new_frame view.draw(check_for_changes = False) File ""/Users/goddard/ucsf/chimerax/ChimeraX.app/Contents/Library/Frameworks/Python.framework/Versions/3.7/lib/python3.7/site- packages/chimerax/graphics/view.py"", line 166, in draw self._draw_scene(camera, drawings) File ""/Users/goddard/ucsf/chimerax/ChimeraX.app/Contents/Library/Frameworks/Python.framework/Versions/3.7/lib/python3.7/site- packages/chimerax/graphics/view.py"", line 240, in _draw_scene draw_transparent(r, transparent_drawings) File ""/Users/goddard/ucsf/chimerax/ChimeraX.app/Contents/Library/Frameworks/Python.framework/Versions/3.7/lib/python3.7/site- packages/chimerax/graphics/drawing.py"", line 1434, in draw_transparent lambda: _draw_multiple(drawings, r, Drawing.TRANSPARENT_DRAW_PASS)) File ""/Users/goddard/ucsf/chimerax/ChimeraX.app/Contents/Library/Frameworks/Python.framework/Versions/3.7/lib/python3.7/site- packages/chimerax/graphics/opengl.py"", line 1170, in draw_transparent draw() File ""/Users/goddard/ucsf/chimerax/ChimeraX.app/Contents/Library/Frameworks/Python.framework/Versions/3.7/lib/python3.7/site- packages/chimerax/graphics/drawing.py"", line 1434, in lambda: _draw_multiple(drawings, r, Drawing.TRANSPARENT_DRAW_PASS)) File ""/Users/goddard/ucsf/chimerax/ChimeraX.app/Contents/Library/Frameworks/Python.framework/Versions/3.7/lib/python3.7/site- packages/chimerax/graphics/drawing.py"", line 1440, in _draw_multiple d.draw(renderer, draw_pass) File ""/Users/goddard/ucsf/chimerax/ChimeraX.app/Contents/Library/Frameworks/Python.framework/Versions/3.7/lib/python3.7/site- packages/chimerax/map/image3d.py"", line 750, in draw bi.draw(renderer, draw_pass) File ""/Users/goddard/ucsf/chimerax/ChimeraX.app/Contents/Library/Frameworks/Python.framework/Versions/3.7/lib/python3.7/site- packages/chimerax/map/image3d.py"", line 1342, in draw Image3d.draw(self, renderer, draw_pass) File ""/Users/goddard/ucsf/chimerax/ChimeraX.app/Contents/Library/Frameworks/Python.framework/Versions/3.7/lib/python3.7/site- packages/chimerax/map/image3d.py"", line 760, in draw pd = self._update_planes(renderer) File ""/Users/goddard/ucsf/chimerax/ChimeraX.app/Contents/Library/Frameworks/Python.framework/Versions/3.7/lib/python3.7/site- packages/chimerax/map/image3d.py"", line 525, in _update_planes pd = self._update_3d_texture_planes(view_dir) File ""/Users/goddard/ucsf/chimerax/ChimeraX.app/Contents/Library/Frameworks/Python.framework/Versions/3.7/lib/python3.7/site- packages/chimerax/map/image3d.py"", line 577, in _update_3d_texture_planes pd = self._texture_3d_planes() File ""/Users/goddard/ucsf/chimerax/ChimeraX.app/Contents/Library/Frameworks/Python.framework/Versions/3.7/lib/python3.7/site- packages/chimerax/map/image3d.py"", line 587, in _texture_3d_planes pd = Texture3dPlanes(self) File ""/Users/goddard/ucsf/chimerax/ChimeraX.app/Contents/Library/Frameworks/Python.framework/Versions/3.7/lib/python3.7/site- packages/chimerax/map/image3d.py"", line 1122, in __init__ self._fill_textures() File ""/Users/goddard/ucsf/chimerax/ChimeraX.app/Contents/Library/Frameworks/Python.framework/Versions/3.7/lib/python3.7/site- packages/chimerax/map/image3d.py"", line 1215, in _fill_textures self._fill_texture_blend(t) File ""/Users/goddard/ucsf/chimerax/ChimeraX.app/Contents/Library/Frameworks/Python.framework/Versions/3.7/lib/python3.7/site- packages/chimerax/map/image3d.py"", line 1265, in _fill_texture_blend b.blend3d(vap.texture, modulation_color, self.texture) File ""/Users/goddard/ucsf/chimerax/ChimeraX.app/Contents/Library/Frameworks/Python.framework/Versions/3.7/lib/python3.7/site- packages/chimerax/graphics/opengl.py"", line 1868, in blend3d r.draw_background() File ""/Users/goddard/ucsf/chimerax/ChimeraX.app/Contents/Library/Frameworks/Python.framework/Versions/3.7/lib/python3.7/site- packages/chimerax/graphics/opengl.py"", line 1096, in draw_background GL.glClear(flags) File ""src/errorchecker.pyx"", line 58, in OpenGL_accelerate.errorchecker._ErrorChecker.glCheckError OpenGL.error.GLError: GLError( err = 1286, description = b'invalid framebuffer operation', baseOperation = glClear, cArguments = (16640,) ) Exception ignored in: Traceback (most recent call last): File ""/Users/goddard/ucsf/chimerax/ChimeraX.app/Contents/Library/Frameworks/Python.framework/Versions/3.7/lib/python3.7/site- packages/chimerax/graphics/drawing.py"", line 1113, in __del__ self.delete() File ""/Users/goddard/ucsf/chimerax/ChimeraX.app/Contents/Library/Frameworks/Python.framework/Versions/3.7/lib/python3.7/site- packages/chimerax/graphics/drawing.py"", line 1133, in delete raise RuntimeError(""Don't have opengl context needed to delete texture from drawing '%s' because drawing was never drawn"" % self.name) RuntimeError: Don't have opengl context needed to delete texture from drawing 'Image3D 3d texture planes' because drawing was never drawn Exception ignored in: Traceback (most recent call last): File ""/Users/goddard/ucsf/chimerax/ChimeraX.app/Contents/Library/Frameworks/Python.framework/Versions/3.7/lib/python3.7/site- packages/chimerax/graphics/opengl.py"", line 2880, in __del__ raise OpenGLError('OpenGL texture was not deleted before graphics.Texture destroyed') chimerax.graphics.opengl.OpenGLError: OpenGL texture was not deleted before graphics.Texture destroyed > volume #1 region 0,0,23,923,623,23 step 1 imageMode ""full region"" OpenGL version: 4.1 ATI-3.9.15 OpenGL renderer: AMD Radeon Pro Vega 20 OpenGL Engine OpenGL vendor: ATI Technologies Inc.Hardware: Hardware Overview: Model Name: MacBook Pro Model Identifier: MacBookPro15,3 Processor Name: 8-Core Intel Core i9 Processor Speed: 2.4 GHz Number of Processors: 1 Total Number of Cores: 8 L2 Cache (per Core): 256 KB L3 Cache: 16 MB Hyper-Threading Technology: Enabled Memory: 32 GB Boot ROM Version: 1037.120.87.0.0 (iBridge: 17.16.15300.0.0,0) Software: System Software Overview: System Version: macOS 10.15.5 (19F101) Kernel Version: Darwin 19.5.0 Time since boot: 2:48 Graphics/Displays: Intel UHD Graphics 630: Chipset Model: Intel UHD Graphics 630 Type: GPU Bus: Built-In VRAM (Dynamic, Max): 1536 MB Vendor: Intel Device ID: 0x3e9b Revision ID: 0x0002 Automatic Graphics Switching: Supported gMux Version: 5.0.0 Metal: Supported, feature set macOS GPUFamily2 v1 Radeon Pro Vega 20: Chipset Model: Radeon Pro Vega 20 Type: GPU Bus: PCIe PCIe Lane Width: x8 VRAM (Total): 4 GB Vendor: AMD (0x1002) Device ID: 0x69af Revision ID: 0x00c0 ROM Revision: 113-D2060I-087 VBIOS Version: 113-D20601MA0T-016 Option ROM Version: 113-D20601MA0T-016 EFI Driver Version: 01.01.087 Automatic Graphics Switching: Supported gMux Version: 5.0.0 Metal: Supported, feature set macOS GPUFamily2 v1 Displays: Color LCD: Display Type: Built-In Retina LCD Resolution: 2880 x 1800 Retina Framebuffer Depth: 24-Bit Color (ARGB8888) Main Display: Yes Mirror: Off Online: Yes Automatically Adjust Brightness: No Connection Type: Internal PyQt version: 5.12.3 Compiled Qt version: 5.12.4 Runtime Qt version: 5.12.8 }}} " defect closed normal Volume Data fixed all ChimeraX