ChimeraX-Related Software ChimeraX home icon

See also: ChimeraX Toolshed

AutoEMage automates cryo-electron (cryo-EM) microscopy file transfer, motion correction, CTF estimation, image display, outlier detection, particle picking, 2D classification, class ranking, and model generation. It uses MotionCor2, CTFFIND4, UCSF ChimeraX, RELION, and IMOD, and is described in Cheng et al., J Appl Cryst 56:1865 (2023).
ContinuousFlex is a plugin to Scipion for continuous conformational flexibility analysis of cryo-EM data. It uses ChimeraX for fitting and visualization, as described in Vuillemot et al., Int J Mol Sci 25:20 (2023).
DiffFit is a ChimeraX plugin that fits atomic structures into cryo-EM maps without requiring the user to place them manually beforehand. It uses the GPU for fast performance and can fit multiple components of a larger complex one by one, each time subtracting the density where a component has already been fit. The fast performance of DiffFit allows evaluating the fit of many hundreds of candidate structures into unknown density, for example, in electron tomography. See the video and paper by Luo et al. (preprint 2024, arXiv:2404.02465 [q-bio.QM]).
DomainFit is a program for automated domain-level protein identification from cryo-EM maps at resolutions > 4 Å. It uses ChimeraX to fit AI-predicted domain structures (such as from AlphaFold) into the maps and cluster the fits, and then it performs statistical analyses to try to identify which proteins form the density. See the example and the paper by Gao et al. (preprint 2023, DOI: 10.1101/2023.11.28.569001).
MitoTNT builds on MitoGraph segmentation and ChimeraX visualization for the analysis of mitochondrial networks in 4D live-cell fluorescence microscopy data, as described in Wang et al., PLoS Comput Biol:e101160 (2023).
MorphOT morphs density maps using shape interpolation based on optimal transport, as described in Ecoffet et al., Bioinformatics:btaa1019 (2020).
PTM-Psi is a Python package that includes a ChimeraX plugin to replace amino acid sidechains with certain post-translationally modified (PTM) forms, as described in Mejia-Rodriquez et al., Protein Sci:e4822 (2023). The initial library includes several common cysteine PTMs, and a broader range of types may be offered in the future.
pyCapsid is a Python package to extract quasi-rigid domains in protein shells and other macromolecular complexes, with output including a script for ChimeraX visualization. It is available as a Google Colab notebook and as code on Github for those who wish to install it locally. See the online tutorial and Brown et al., Bioinformatics 40:btad761 (2024).
pyLattice is a library of Python modules, Jupyter notebooks, and Matlab routines that operate together to form an image analysis pipeline for lattice light-sheet microscopy, as described in Schöneberg et al., Mol Biol Cell 29:2959 (2018). It provides customized input for 4D (3D + time) visualization in ChimeraX.
Python script to convert Relion star file into ChimeraX visualization command script.
RNArtist allows designing RNA 2D structures interactively and using UCSF ChimeraX (or UCSF Chimera) to explore 3D architectures. RNArtist is developed by Fabrice Jossinet at the Institut de Biologie Moléculaire et Cellulaire, Strasbourg.

ScanNet Server
The ScanNet server predicts protein-binding sites for structures in the PDB, the AlphaFold Database, or uploaded from a local file. The method is described in Tubiana et al., Nat Methods 19:730 (2022) and the web server specifically in Tubiana et al., J Mol Biol 434:167758 (2022). Results are emailed to the user as a zip file containing:
  • the PDB file with binding site probabilities in the B-factor field
  • csv file containing the binding site probabilities
  • ChimeraX command script (.cxc) for visualizing the results (right-click → open the file with ChimeraX)
  • Chimera python script (.py) for visualizing the results (right-click → open the file with Chimera)

SymProFold combines AlphaFold-Multimer predictions with general symmetry considerations to predict symmetric protein assemblies such as bacterial S-layers and viral capsids. It creates an mmCIF file containing the primitive unit cell and symmetry information of the predicted assembly. The related tool Domain_Separator separates an atomic structure into structural domains. Both tools use ChimeraX methods and libraries and are described in Buhlheller et al. (preprint 2023, DOI: 10.21203/

Wiggle is a ChimeraX plugin for visualizing conformational heterogeneity within cryo-EM data, as calculated by cryoDRGN or by cryoSPARC 3D variability analysis; see the user interface overview and other video tutorials. The cryoDRGN and cryoSPARC analyses depend on a CUDA-accelerated GPU, and Wiggle is currently experimental (hence not yet available on the ChimeraX Toolshed).
xiView is a web-based visualisation tool for the analysis of cross-linking mass spectrometry results, as described in Graham et al., bioRxiv (2019). 3D export options include UCSF ChimeraX pseudobond files.