AlphaFold is an artificial intelligence method for predicting
protein structures. With the
AlphaFold tool
or command,
ChimeraX can search for and load predicted structures
from the freely available AlphaFold Database,
automatically coloring them by confidence value:
- 100
to 90
– high accuracy
- 90
to 70
– backbone accuracy
- 70
to 50
– low confidence, caution
- 50
to 0
– should not be interpreted, may be disordered
The figure shows the predicted structure of
UniProt
entry TOM40_HUMAN, a channel protein needed
to import other proteins into mitochondria.
See the command file tom40.cxc
for fetching data and other setup (background color, etc.).
Opening a sequence from
UniProt
also opens a
dialog
in which its annotations or “features”
can be clicked to highlight those regions in both the sequence and
the associated 3D structure. The low-confidence part of this structure
(orange and red) maps to compositionally biased and likely disordered
regions near the N-terminus of the sequence.
More features...